cnn

Summary

Gene Symbol: cnn
Description: centrosomin
Alias: CG 4832, CG18370, CG4832, CNN, Cnn, Cnn1, Dmel\CG4832, cen, mat(2)syn-C, mat(2)synHK21, mfs, obv, centrosomin, CG4832-PA, CG4832-PB, CG4832-PD, CG4832-PE, CG4832-PG, CG4832-PH, CG4832-PI, CG4832-PJ, CG4832-PK, CG4832-PL, CG4832-PM, CG4832-PN, centrosmin, cnn-PA, cnn-PB, cnn-PD, cnn-PE, cnn-PG, cnn-PH, cnn-PI, cnn-PJ, cnn-PK, cnn-PL, cnn-PM, cnn-PN, maternal effect syncytial blastoderm arrest C, oblivious
Species: fruit fly

Top Publications

  1. Giet R, McLean D, Descamps S, Lee M, Raff J, Prigent C, et al. Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules. J Cell Biol. 2002;156:437-51 pubmed
  2. Conduit P, Brunk K, Dobbelaere J, Dix C, Lucas E, Raff J. Centrioles regulate centrosome size by controlling the rate of Cnn incorporation into the PCM. Curr Biol. 2010;20:2178-86 pubmed publisher
    ..we show that the conserved Drosophila PCM protein Cnn exhibits an unusual dynamic behavior, because Cnn molecules only incorporate into the PCM closest to the centrioles ..
  3. Inaba M, Yuan H, Salzmann V, Fuller M, Yamashita Y. E-cadherin is required for centrosome and spindle orientation in Drosophila male germline stem cells. PLoS ONE. 2010;5:e12473 pubmed publisher
    ..We propose that E-cadherin orchestrates multiple aspects of stem cell behavior, including polarization of stem cells toward the stem cell-niche interface and adhesion of stem cells to the niche supporting cells. ..
  4. Basto R, Brunk K, Vinadogrova T, Peel N, Franz A, Khodjakov A, et al. Centrosome amplification can initiate tumorigenesis in flies. Cell. 2008;133:1032-42 pubmed publisher
    ..Thus, centrosome amplification can initiate tumorigenesis in flies. ..
  5. Zhang J, Megraw T. Proper recruitment of gamma-tubulin and D-TACC/Msps to embryonic Drosophila centrosomes requires Centrosomin Motif 1. Mol Biol Cell. 2007;18:4037-49 pubmed
    ..Although the individual binding steps in centrosome maturation are largely unknown, Centrosomin (Cnn) is an essential mitotic centrosome component required for assembly of all other known pericentriolar ..
  6. Bettencourt Dias M, Rodrigues Martins A, Carpenter L, Riparbelli M, Lehmann L, Gatt M, et al. SAK/PLK4 is required for centriole duplication and flagella development. Curr Biol. 2005;15:2199-207 pubmed
    ..Drosophila cells tolerate the lack of centrioles and undertake mitosis but cannot form basal bodies and hence flagella. Human cells depleted of SAK show error-prone mitosis, likely to underlie its tumor-suppressor role. ..
  7. Basto R, Lau J, Vinogradova T, Gardiol A, Woods C, Khodjakov A, et al. Flies without centrioles. Cell. 2006;125:1375-86 pubmed
    ..Thus, centrioles are essential for the formation of centrosomes, cilia, and flagella, but, remarkably, they are not essential for most aspects of Drosophila development. ..
  8. Gopalakrishnan J, Mennella V, Blachon S, Zhai B, Smith A, Megraw T, et al. Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome. Nat Commun. 2011;2:359 pubmed publisher
    ..In this study, we show that Sas-4 provides a scaffold for cytoplasmic complexes (named S-CAP), which include CNN, Asl and D-PLP, proteins that are all found in the centrosomes at the vicinity of the centriole...
  9. Conduit P, Raff J. Cnn dynamics drive centrosome size asymmetry to ensure daughter centriole retention in Drosophila neuroblasts. Curr Biol. 2010;20:2187-92 pubmed publisher
    ..evidence that centrosome size asymmetry in Drosophila neuroblasts is generated by the differential regulation of Cnn incorporation into the PCM at mother and daughter centrioles...

More Information

Publications79

  1. Castellanos E, Dominguez P, Gonzalez C. Centrosome dysfunction in Drosophila neural stem cells causes tumors that are not due to genome instability. Curr Biol. 2008;18:1209-14 pubmed publisher
    ..We propose that such tumors might be caused by impaired asymmetric division of neural stem cells [4]. These results show that centrosome loss, far from being innocuous, is a potentially dangerous condition in flies...
  2. Giansanti M, Belloni G, Gatti M. Rab11 is required for membrane trafficking and actomyosin ring constriction in meiotic cytokinesis of Drosophila males. Mol Biol Cell. 2007;18:5034-47 pubmed
    ..We propose that Gio and Fwd mediate Rab11 recruitment at the cleavage furrow and that Rab11 facilitates targeted membrane delivery to the advancing furrow. ..
  3. Pérez Mongiovi D, Malmanche N, Bousbaa H, Sunkel C. Maternal expression of the checkpoint protein BubR1 is required for synchrony of syncytial nuclear divisions and polar body arrest in Drosophila melanogaster. Development. 2005;132:4509-20 pubmed
    ..Our results suggest that BubR1 is required for normal synchrony and progression of syncytial nuclei through mitosis and to maintain the mitotic arrest of the polar body chromosomes after completion of meiosis. ..
  4. Mottier Pavie V, Megraw T. Drosophila bld10 is a centriolar protein that regulates centriole, basal body, and motile cilium assembly. Mol Biol Cell. 2009;20:2605-14 pubmed publisher
    ..These results show that Drosophila Bld10 is required for centriole and axoneme assembly to confer cilium motility. ..
  5. Bonaccorsi S, Giansanti M, Gatti M. Spindle assembly in Drosophila neuroblasts and ganglion mother cells. Nat Cell Biol. 2000;2:54-6 pubmed
  6. Goshima G, Mayer M, Zhang N, Stuurman N, Vale R. Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle. J Cell Biol. 2008;181:421-9 pubmed publisher
    ..Our results suggest that an important mitotic function for gamma-tubulin may lie within the spindle, where augmin and gamma-tubulin function cooperatively to amplify the number of microtubules. ..
  7. Conduit P, Feng Z, Richens J, Baumbach J, Wainman A, Bakshi S, et al. The centrosome-specific phosphorylation of Cnn by Polo/Plk1 drives Cnn scaffold assembly and centrosome maturation. Dev Cell. 2014;28:659-69 pubmed publisher
    ..How centrosomes mature remains mysterious. Here, we identify a domain in Drosophila Cnn that appears to be phosphorylated by Polo/Plk1 specifically at centrosomes during mitosis...
  8. Rebollo E, Sampaio P, Januschke J, Llamazares S, Varmark H, Gonzalez C. Functionally unequal centrosomes drive spindle orientation in asymmetrically dividing Drosophila neural stem cells. Dev Cell. 2007;12:467-74 pubmed
    ..Apical aster maintenance requires the function of Pins. These results reveal that spindle orientation in Drosophila larval NBs is determined very early in the cell cycle, and is mediated by asymmetric centrosome function. ..
  9. Rusan N, Peifer M. A role for a novel centrosome cycle in asymmetric cell division. J Cell Biol. 2007;177:13-20 pubmed
    ..We propose a two-step mechanism to ensure faithful spindle positioning: the novel centrosome cycle produces a single interphase MTOC, coarsely aligning the spindle, and spindle-cortex interactions refine this alignment...
  10. Li K, Kaufman T. The homeotic target gene centrosomin encodes an essential centrosomal component. Cell. 1996;85:585-96 pubmed
    The centrosomin (cnn) gene encodes a protein associated with mitotic centrosomes in Drosophila melanogaster and is a target of homeotic gene regulation. Here we report that CNN is an essential component of the centrosome...
  11. Cai Y, Yu F, Lin S, Chia W, Yang X. Apical complex genes control mitotic spindle geometry and relative size of daughter cells in Drosophila neuroblast and pI asymmetric divisions. Cell. 2003;112:51-62 pubmed
    ..In sensory organ precursors, Bazooka/DaPKC and Pins/G alpha i localize to opposite sides of the cortex and function in opposition to generate a symmetric spindle. ..
  12. Megraw T, Li K, Kao L, Kaufman T. The centrosomin protein is required for centrosome assembly and function during cleavage in Drosophila. Development. 1999;126:2829-39 pubmed
    b>Centrosomin is a 150 kDa centrosomal protein of Drosophila melanogaster. To study the function of Centrosomin in the centrosome, we have recovered mutations that are viable but male and female sterile (cnnmfs)...
  13. Gonzalez C, Tavosanis G, Mollinari C. Centrosomes and microtubule organisation during Drosophila development. J Cell Sci. 1998;111 ( Pt 18):2697-706 pubmed
    ..In this review we present some of the many examples which illustrate how centrosomes and microtubule organisation changes through development in Drosophila and discuss some of its implications. ..
  14. Bucciarelli E, Pellacani C, Naim V, Palena A, Gatti M, Somma M. Drosophila Dgt6 interacts with Ndc80, Msps/XMAP215, and gamma-tubulin to promote kinetochore-driven MT formation. Curr Biol. 2009;19:1839-45 pubmed publisher
    ..Dgt6 may also promote elongation of kinetochore-driven k-fibers through its interaction with gamma-tubulin and Msps. ..
  15. Brust Mascher I, Sommi P, Cheerambathur D, Scholey J. Kinesin-5-dependent poleward flux and spindle length control in Drosophila embryo mitosis. Mol Biol Cell. 2009;20:1749-62 pubmed publisher
    ..Finally, KLP61F activity contributes to normal chromosome congression, kinetochore spacing, and anaphase A rates. Thus, a KLP61F-driven sliding filament mechanism contributes to multiple aspects of mitosis in this system. ..
  16. Rogers G, Rusan N, Peifer M, Rogers S. A multicomponent assembly pathway contributes to the formation of acentrosomal microtubule arrays in interphase Drosophila cells. Mol Biol Cell. 2008;19:3163-78 pubmed publisher
    ..Taken together, these results modify our view of the cycle of centrosome function and reveal a multi-component acentrosomal microtubule assembly pathway to establish interphase microtubule arrays in Drosophila. ..
  17. Yamashita Y, Jones D, Fuller M. Orientation of asymmetric stem cell division by the APC tumor suppressor and centrosome. Science. 2003;301:1547-50 pubmed
  18. Meireles A, Fisher K, Colombié N, Wakefield J, Ohkura H. Wac: a new Augmin subunit required for chromosome alignment but not for acentrosomal microtubule assembly in female meiosis. J Cell Biol. 2009;184:777-84 pubmed publisher
    ..For the first time, this study establishes the role of an Augmin subunit in developing organisms and provides an insight into the architecture of the complex. ..
  19. Giansanti M, Gatti M, Bonaccorsi S. The role of centrosomes and astral microtubules during asymmetric division of Drosophila neuroblasts. Development. 2001;128:1137-45 pubmed
    ..This suggests that astral microtubules mediate proper spindle rotation during neuroblast division. ..
  20. Peel N, Stevens N, Basto R, Raff J. Overexpressing centriole-replication proteins in vivo induces centriole overduplication and de novo formation. Curr Biol. 2007;17:834-43 pubmed
    ..Here, we investigate the effects of overexpressing the three proteins known to be required for centriole replication in Drosophila-DSas-6, DSas-4, and Sak...
  21. Kramer J, Hawley R. The spindle-associated transmembrane protein Axs identifies a membranous structure ensheathing the meiotic spindle. Nat Cell Biol. 2003;5:261-3 pubmed
    ..In both meiotic and mitotic cells, Axs is recruited to the microtubules of assembling spindles. We propose that Axs and the sheath represent novel mediators of meiotic spindle assembly and chromosome segregation. ..
  22. Sheng X, Matunis E. Live imaging of the Drosophila spermatogonial stem cell niche reveals novel mechanisms regulating germline stem cell output. Development. 2011;138:3367-76 pubmed publisher
    ..Thus, stereotypically oriented stem cell divisions are not always correlated with an asymmetric outcome in cell fate, and changes in stem cell output are governed by altered signals in response to tissue requirements. ..
  23. Moutinho Pereira S, Debec A, Maiato H. Microtubule cytoskeleton remodeling by acentriolar microtubule-organizing centers at the entry and exit from mitosis in Drosophila somatic cells. Mol Biol Cell. 2009;20:2796-808 pubmed publisher
    ..MTs originate from acentriolar microtubule-organizing centers (aMTOCs) that contain gamma-tubulin and require Centrosomin (Cnn) for normal architecture and function...
  24. Megraw T, Kao L, Kaufman T. Zygotic development without functional mitotic centrosomes. Curr Biol. 2001;11:116-20 pubmed
    ..Here we show that in Drosophila a centrosomin (cnn) null mutant, which fails to assemble fully functional mitotic centrosomes and has few or no detectable ..
  25. Emery G, Hutterer A, Berdnik D, Mayer B, Wirtz Peitz F, Gaitan M, et al. Asymmetric Rab 11 endosomes regulate delta recycling and specify cell fate in the Drosophila nervous system. Cell. 2005;122:763-73 pubmed
    ..Our results suggest that cells can regulate signaling pathways and influence their developmental fate by inhibiting the formation of individual endocytic compartments...
  26. Januschke J, Reina J, Llamazares S, Bertran T, Rossi F, Roig J, et al. Centrobin controls mother-daughter centriole asymmetry in Drosophila neuroblasts. Nat Cell Biol. 2013;15:241-8 pubmed publisher
    ..CNB co-precipitates with a set of centrosomal proteins that include γ-TUB, ANA2, CNN, SAS-4, ASL, DGRIP71, POLO and SAS-6...
  27. Roth T, Chiang C, Inaba M, Yuan H, Salzmann V, Roth C, et al. Centrosome misorientation mediates slowing of the cell cycle under limited nutrient conditions in Drosophila male germline stem cells. Mol Biol Cell. 2012;23:1524-32 pubmed publisher
    ..We propose that centrosome misorientation serves as a mediator that transduces nutrient information into stem cell proliferation, providing a previously unappreciated mechanism of stem cell regulation in response to nutrient conditions...
  28. Raynaud Messina B, Mazzolini L, Moisand A, Cirinesi A, Wright M. Elongation of centriolar microtubule triplets contributes to the formation of the mitotic spindle in gamma-tubulin-depleted cells. J Cell Sci. 2004;117:5497-507 pubmed
    ..Despite the marked depletion of gamma-tubulin, the pericentriolar proteins CP190 and centrosomin were recruited to the spindle pole(s), where they formed three or four dots, suggesting the presence of several ..
  29. Lucas E, Raff J. Maintaining the proper connection between the centrioles and the pericentriolar matrix requires Drosophila centrosomin. J Cell Biol. 2007;178:725-32 pubmed
    ..We show that the centrioles in Drosophila embryos that lack the centrosomal protein Centrosomin (Cnn) can recruit PCM components but cannot maintain a proper attachment to the PCM...
  30. Heuer J, Li K, Kaufman T. The Drosophila homeotic target gene centrosomin (cnn) encodes a novel centrosomal protein with leucine zippers and maps to a genomic region required for midgut morphogenesis. Development. 1995;121:3861-76 pubmed
    ..We present here the characterization of centrosomin (cnn), one of the target genes isolated using this approach...
  31. Wei H, Rollins J, Fabian L, Hayes M, Polevoy G, Bazinet C, et al. Depletion of plasma membrane PtdIns(4,5)P2 reveals essential roles for phosphoinositides in flagellar biogenesis. J Cell Sci. 2008;121:1076-84 pubmed publisher
    ..They further suggest that phosphoinositides play evolutionarily conserved roles in flagella and cilia, across phyla and in structurally diverse cell types. ..
  32. Bonaccorsi S, Giansanti M, Gatti M. Spindle self-organization and cytokinesis during male meiosis in asterless mutants of Drosophila melanogaster. J Cell Biol. 1998;142:751-61 pubmed
    ..However, immunostaining with antibodies directed either to gamma tubulin or centrosomin revealed that these proteins do not accumulate in the centrosomes, as occurs in wild-type...
  33. Blachon S, Cai X, Roberts K, Yang K, Polyanovsky A, Church A, et al. A proximal centriole-like structure is present in Drosophila spermatids and can serve as a model to study centriole duplication. Genetics. 2009;182:133-44 pubmed publisher
    ..We propose that, during the evolution of insects, the proximal centriole was simplified by eliminating the later steps in centriole assembly. The PCL may provide a unique model to study early steps of centriole formation. ..
  34. Blachon S, Gopalakrishnan J, Omori Y, Polyanovsky A, Church A, Nicastro D, et al. Drosophila asterless and vertebrate Cep152 Are orthologs essential for centriole duplication. Genetics. 2008;180:2081-94 pubmed publisher
    ..The clear absence of several centriolar markers in mecD mutants suggests that Asl is critical early in centriole duplication...
  35. de Anda F, Pollarolo G, Da Silva J, Camoletto P, Feiguin F, Dotti C. Centrosome localization determines neuronal polarity. Nature. 2005;436:704-8 pubmed
    ..We conclude that asymmetric centrosome-mediated dynamics in the early post-mitotic stage instruct neuronal polarity, implying that pre-mitotic mechanisms with a role in division orientation may in turn participate in this event. ..
  36. Deshpande G, Calhoun G, Schedl P. Drosophila argonaute-2 is required early in embryogenesis for the assembly of centric/centromeric heterochromatin, nuclear division, nuclear migration, and germ-cell formation. Genes Dev. 2005;19:1680-5 pubmed
    ..The aberrations in the nuclear division cycle are correlated with defects in the formation of centric/centromeric heterochromatin and point to a failure in the assembly of functional centromeres. ..
  37. Fu J, Glover D. Structured illumination of the interface between centriole and peri-centriolar material. Open Biol. 2012;2:120104 pubmed publisher
    ..b>Centrosomin (Cnn) and ?-tubulin associate with this outer tube in G2 cells and, upon mitotic entry, Polo activity is ..
  38. Li K, Xu E, Cecil J, Turner F, Megraw T, Kaufman T. Drosophila centrosomin protein is required for male meiosis and assembly of the flagellar axoneme. J Cell Biol. 1998;141:455-67 pubmed
    ..b>Centrosomin is a protein localized to the mitotic centrosomes in Drosophila melanogaster...
  39. Siller K, Serr M, Steward R, Hays T, Doe C. Live imaging of Drosophila brain neuroblasts reveals a role for Lis1/dynactin in spindle assembly and mitotic checkpoint control. Mol Biol Cell. 2005;16:5127-40 pubmed
    ..We conclude that Lis1/dynactin act together to regulate multiple, independent functions in mitotic cells, including spindle formation and cell cycle checkpoint release. ..
  40. Wainman A, Buster D, Duncan T, Metz J, Ma A, Sharp D, et al. A new Augmin subunit, Msd1, demonstrates the importance of mitotic spindle-templated microtubule nucleation in the absence of functioning centrosomes. Genes Dev. 2009;23:1876-81 pubmed publisher
    ..Importantly, however, in the absence of centrosomes, microtubule nucleation from within the spindle becomes essential. Thus, the Augmin complex has a crucial role in the development of the fly. ..
  41. Wang H, Somers G, Bashirullah A, Heberlein U, Yu F, Chia W. Aurora-A acts as a tumor suppressor and regulates self-renewal of Drosophila neuroblasts. Genes Dev. 2006;20:3453-63 pubmed
    ..We have identified a novel mechanism for controlling the balance between self-renewal and neuronal differentiation during the asymmetric division of Drosophila larval NBs. ..
  42. Giansanti M, Bucciarelli E, Bonaccorsi S, Gatti M. Drosophila SPD-2 is an essential centriole component required for PCM recruitment and astral-microtubule nucleation. Curr Biol. 2008;18:303-9 pubmed publisher
    ..localization at the centrosome requires the wild-type activity of Asl but is independent of the function of D-PLP, Cnn, gamma-tubulin, DGrip91, and D-TACC...
  43. Baker J, Adhikarakunnathu S, Kernan M. Mechanosensory-defective, male-sterile unc mutants identify a novel basal body protein required for ciliogenesis in Drosophila. Development. 2004;131:3411-22 pubmed
    ..UNC is thus specifically required for normal ciliogenesis. Its localization is an early marker for the centriole-basal body transition, a central but enigmatic event in eukaryotic cell differentiation. ..
  44. Riparbelli M, Callaini G, Glover D, Avides M. A requirement for the Abnormal Spindle protein to organise microtubules of the central spindle for cytokinesis in Drosophila. J Cell Sci. 2002;115:913-22 pubmed
    ..Upon fertilisation, Asp is also recruited to the MTOC that nucleates the sperm aster. Asp is required for growth of the microtubules of the sperm aster, which in asp mutants remains diminutive and so prevents migration of the pronuclei. ..
  45. Rodrigues Martins A, Riparbelli M, Callaini G, Glover D, Bettencourt Dias M. Revisiting the role of the mother centriole in centriole biogenesis. Science. 2007;316:1046-50 pubmed
    ..The mother centriole is not a bona fide template but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly...
  46. Bolivar J, Huynh J, Lopez Schier H, Gonzalez C, St Johnston D, González Reyes A. Centrosome migration into the Drosophila oocyte is independent of BicD and egl, and of the organisation of the microtubule cytoskeleton. Development. 2001;128:1889-97 pubmed
    ..Thus, centrosome migration is independent of the organisation of the microtubule cytoskeleton, and seems to depend instead on the polarity of the fusome. ..
  47. Berdnik D, Knoblich J. Drosophila Aurora-A is required for centrosome maturation and actin-dependent asymmetric protein localization during mitosis. Curr Biol. 2002;12:640-7 pubmed
    ..aurA(37) mutant cells also fail to recruit Centrosomin (Cnn) and gamma-Tubulin to centrosomes during mitosis, leading to spindle morphology defects...
  48. Martinez Campos M, Basto R, Baker J, Kernan M, Raff J. The Drosophila pericentrin-like protein is essential for cilia/flagella function, but appears to be dispensable for mitosis. J Cell Biol. 2004;165:673-83 pubmed
    ..Moreover, the flagella in mutant sperm are nonmotile. Thus, D-PLP is essential for the formation of functional cilia and flagella in flies. ..
  49. Pimenta Marques A, Tostões R, Marty T, Barbosa V, Lehmann R, Martinho R. Differential requirements of a mitotic acetyltransferase in somatic and germ line cells. Dev Biol. 2008;323:197-206 pubmed publisher
    ..Surprisingly, during oogenesis we cannot detect mitotic defects in germ line cells mutant for san. We hypothesize the female germ line stem cells have differential requirements for mitotic sister chromatid cohesion. ..
  50. Mottier Pavie V, Cenci G, Vernì F, Gatti M, Bonaccorsi S. Phenotypic analysis of misato function reveals roles of noncentrosomal microtubules in Drosophila spindle formation. J Cell Sci. 2011;124:706-17 pubmed publisher
    ..Collectively, these results indicate that kinetochore-driven MT formation is an essential process for proper spindle assembly in Drosophila somatic cells. ..
  51. Donaldson M, Tavares A, Ohkura H, Deak P, Glover D. Metaphase arrest with centromere separation in polo mutants of Drosophila. J Cell Biol. 2001;153:663-76 pubmed
    ..The mutant mitotic spindles are anastral and have assembled upon centrosomes that are associated with Centrosomin and the abnormal spindle protein (Asp), but neither with gamma-tubulin nor CP190...
  52. Schnorrer F, Luschnig S, Koch I, NUSSLEIN VOLHARD C. Gamma-tubulin37C and gamma-tubulin ring complex protein 75 are essential for bicoid RNA localization during drosophila oogenesis. Dev Cell. 2002;3:685-96 pubmed
    ..At stage 10b, we find gammaTub37C and Dgrip75 anteriorly concentrated and propose the formation of a microtubule-organizing center at the anterior pole of the oocyte...
  53. Moritz M, Zheng Y, Alberts B, Oegema K. Recruitment of the gamma-tubulin ring complex to Drosophila salt-stripped centrosome scaffolds. J Cell Biol. 1998;142:775-86 pubmed
    ..centrosomes with 2 M KI generates salt-resistant scaffolds that lack the centrosomal proteins CP190, CP60, centrosomin, and gamma-tubulin...
  54. Barbosa V, Yamamoto R, Henderson D, Glover D. Mutation of a Drosophila gamma tubulin ring complex subunit encoded by discs degenerate-4 differentially disrupts centrosomal protein localization. Genes Dev. 2000;14:3126-39 pubmed
    ..b>Centrosomin (CNN) remains in broad discrete bodies but only at the focused poles of such spindles, whereas Asp (abnormal ..
  55. Zhang G, Breuer M, Förster A, Egger Adam D, Wodarz A. Mars, a Drosophila protein related to vertebrate HURP, is required for the attachment of centrosomes to the mitotic spindle during syncytial nuclear divisions. J Cell Sci. 2009;122:535-45 pubmed publisher
    ..We propose that Mars is an important linker between the spindle and the centrosomes that is required for proper spindle organization during the rapid mitotic cycles in early embryogenesis. ..
  56. Buffin E, Emre D, Karess R. Flies without a spindle checkpoint. Nat Cell Biol. 2007;9:565-72 pubmed
  57. Nystul T, Spradling A. Breaking out of the mold: diversity within adult stem cells and their niches. Curr Opin Genet Dev. 2006;16:463-8 pubmed
    ..Understanding how stem cells and their progeny are controlled will illuminate fundamental biological mechanisms that govern the construction and maintenance of tissues within metazoan animals. ..
  58. Megraw T, Kilaru S, Turner F, Kaufman T. The centrosome is a dynamic structure that ejects PCM flares. J Cell Sci. 2002;115:4707-18 pubmed
    The Drosophila Centrosomin (Cnn) protein is an essential core component of centrosomes in the early embryo...
  59. Sibon O, Kelkar A, Lemstra W, Theurkauf W. DNA-replication/DNA-damage-dependent centrosome inactivation in Drosophila embryos. Nat Cell Biol. 2000;2:90-5 pubmed
    ..We propose that centrosome inactivation is part of a damage-control system that blocks chromosome segregation when replication/damage checkpoint control fails. ..
  60. Deak P, Donaldson M, Glover D. Mutations in mákos, a Drosophila gene encoding the Cdc27 subunit of the anaphase promoting complex, enhance centrosomal defects in polo and are suppressed by mutations in twins/aar, which encodes a regulatory subunit of PP2A. J Cell Sci. 2003;116:4147-58 pubmed
    ..Thus the APC/C might normally be required to inactivate wild-type twins/aar gene product. ..
  61. Yu F, Wang H, Qian H, Kaushik R, Bownes M, Yang X, et al. Locomotion defects, together with Pins, regulates heterotrimeric G-protein signaling during Drosophila neuroblast asymmetric divisions. Genes Dev. 2005;19:1341-53 pubmed
    ..Thus, Loco can potentially regulate heterotrimeric G-protein signaling via two distinct modes of action during Drosophila neuroblast asymmetric divisions. ..
  62. Wakefield J, Bonaccorsi S, Gatti M. The drosophila protein asp is involved in microtubule organization during spindle formation and cytokinesis. J Cell Biol. 2001;153:637-48 pubmed
    ..Our data also suggest that Asp has a role in the formation of the central spindle. The inability of asp mutants to correctly organize the central spindle leads to disruption of the contractile ring machinery and failure in cytokinesis. ..
  63. Colombié N, Verollet C, Sampaio P, Moisand A, Sunkel C, Bourbon H, et al. The Drosophila gamma-tubulin small complex subunit Dgrip84 is required for structural and functional integrity of the spindle apparatus. Mol Biol Cell. 2006;17:272-82 pubmed
    ..The nature of a minimal gamma-tubulin complex necessary for proper microtubule organization in the metazoans is discussed. ..
  64. Terada Y, Uetake Y, Kuriyama R. Interaction of Aurora-A and centrosomin at the microtubule-nucleating site in Drosophila and mammalian cells. J Cell Biol. 2003;162:757-63 pubmed
    ..Here, we show that Aurora-A specifically binds to the COOH-terminal domain of a Drosophila centrosomal protein, centrosomin (CNN), which has been shown to be important for assembly of mitotic spindles and spindle poles...
  65. Dix C, Raff J. Drosophila Spd-2 recruits PCM to the sperm centriole, but is dispensable for centriole duplication. Curr Biol. 2007;17:1759-64 pubmed
    ..We speculate that the SPD-2 family of proteins might only be absolutely essential for the recruitment of centriole duplication factors and PCM to the centriole(s) that enter the egg with the fertilizing sperm...
  66. Lee C, Andersen R, Cabernard C, Manning L, Tran K, Lanskey M, et al. Drosophila Aurora-A kinase inhibits neuroblast self-renewal by regulating aPKC/Numb cortical polarity and spindle orientation. Genes Dev. 2006;20:3464-74 pubmed
    ..We conclude that Aurora-A and Numb are novel inhibitors of neuroblast self-renewal and that spindle orientation regulates neuroblast self-renewal. ..
  67. Verollet C, Colombié N, Daubon T, Bourbon H, Wright M, Raynaud Messina B. Drosophila melanogaster gamma-TuRC is dispensable for targeting gamma-tubulin to the centrosome and microtubule nucleation. J Cell Biol. 2006;172:517-28 pubmed
    ..In conclusion, gamma-TuRCs are required for assembly of fully functional spindles, but we suggest that gamma-TuSC could be targeted to the centrosomes, which is where basic microtubule assembly activities are maintained. ..
  68. Riparbelli M, Callaini G. Detachment of the basal body from the sperm tail is not required to organize functional centrosomes during Drosophila embryogenesis. Cytoskeleton (Hoboken). 2010;67:251-8 pubmed publisher
    ..If the sperm centriole is a true basal body, then the widespread idea that cells with a primary cilium must resorb the axoneme and transform the basal body into a centriole to enable proper mitosis will have to be re-examined...
  69. Blagden S, Glover D. Polar expeditions--provisioning the centrosome for mitosis. Nat Cell Biol. 2003;5:505-11 pubmed
    ..Centrosomes enlarge dramatically after mitotic entry, when both Aurora A and Polo-like kinases cooperate to recruit additional gamma-tubulin ring complexes and microtubule-associated proteins to assist spindle formation. ..
  70. Varmark H, Llamazares S, Rebollo E, Lange B, Reina J, Schwarz H, et al. Asterless is a centriolar protein required for centrosome function and embryo development in Drosophila. Curr Biol. 2007;17:1735-45 pubmed
    ..In addition, the phenotype of asl-deficient flies reveals that a functional centrosome is required for Drosophila embryo development. ..