Gene Symbol: Chd1
Description: Chromodomain-helicase-DNA-binding protein 1
Alias: CG3733, CHD-1, CHD1, DmCHD1, Dmel\CG3733, chd1, dCHD1, l(2)23Cd, Chromodomain-helicase-DNA-binding protein 1, CG3733-PA, CG3733-PB, CG3733-PC, Chd1-PA, Chd1-PB, Chd1-PC, Chromodomain-helicase-DNA-binding protein, chromo-ATPase/helicase-DNA-binding domain, chromo-ATPase/helicase-DNA-binding protein 1, lethal (2) 23Cd
Species: fruit fly

Top Publications

  1. Kelley D, Stokes D, Perry R. CHD1 interacts with SSRP1 and depends on both its chromodomain and its ATPase/helicase-like domain for proper association with chromatin. Chromosoma. 1999;108:10-25 pubmed
    b>CHD1, an Mr approximately 200,000 protein that contains a chromodomain (C), an ATPase/helicase-like domain (H) and a DNA-binding domain (D), was previously shown to be associated with decompacted interphase chromatin in mammalian cells ..
  2. Radman Livaja M, Quan T, Valenzuela L, Armstrong J, van Welsem T, Kim T, et al. A key role for Chd1 in histone H3 dynamics at the 3' ends of long genes in yeast. PLoS Genet. 2012;8:e1002811 pubmed publisher
    ..Previous studies of the Chd1 subclass of these proteins have implicated them in diverse roles in gene expression including functions during ..
  3. Polo S, Almouzni G. Chromatin assembly: a basic recipe with various flavours. Curr Opin Genet Dev. 2006;16:104-11 pubmed
    ..New data highlight the importance of histone dimers as deposition entities for de novo nucleosome assembly and identify dedicated machineries involved in histone variant deposition. ..
  4. Konev A, Tribus M, Park S, Podhraski V, Lim C, Emelyanov A, et al. CHD1 motor protein is required for deposition of histone variant H3.3 into chromatin in vivo. Science. 2007;317:1087-90 pubmed
    ..Elimination of chromatin remodeling factor CHD1 in Drosophila embryos abolishes incorporation of H3...
  5. Schneiderman J, Sakai A, Goldstein S, Ahmad K. The XNP remodeler targets dynamic chromatin in Drosophila. Proc Natl Acad Sci U S A. 2009;106:14472-7 pubmed publisher
    ..Thus, the XNP focus appears to be a functional genetic element that can contribute to gene silencing throughout the nucleus. ..
  6. Lusser A, Urwin D, Kadonaga J. Distinct activities of CHD1 and ACF in ATP-dependent chromatin assembly. Nat Struct Mol Biol. 2005;12:160-6 pubmed
    b>CHD1 is a chromodomain-containing protein in the SNF2-like family of ATPases. Here we show that CHD1 exists predominantly as a monomer and functions as an ATP-utilizing chromatin assembly factor...
  7. Srinivasan S, Armstrong J, Deuring R, Dahlsveen I, McNeill H, Tamkun J. The Drosophila trithorax group protein Kismet facilitates an early step in transcriptional elongation by RNA Polymerase II. Development. 2005;132:1623-35 pubmed
    ..The levels of elongating Pol II and the elongation factors SPT6 and CHD1 are dramatically reduced on polytene chromosomes from kis mutant larvae...
  8. Petesch S, Lis J. Rapid, transcription-independent loss of nucleosomes over a large chromatin domain at Hsp70 loci. Cell. 2008;134:74-84 pubmed publisher
    ..An RNAi screen of 28 transcription and chromatin-related factors reveals that depletion of heat shock factor, GAGA Factor, or Poly(ADP)-Ribose Polymerase or its activity abolishes the loss of nucleosomes upon Hsp70 activation. ..
  9. Woodage T, Basrai M, Baxevanis A, Hieter P, Collins F. Characterization of the CHD family of proteins. Proc Natl Acad Sci U S A. 1997;94:11472-7 pubmed
    The murine gene CHD1 (MmCHD1) was previously isolated in a search for proteins that bound a DNA promoter element...

More Information


  1. Stokes D, Tartof K, Perry R. CHD1 is concentrated in interbands and puffed regions of Drosophila polytene chromosomes. Proc Natl Acad Sci U S A. 1996;93:7137-42 pubmed
    ..We now report on the characterization of the Drosophila melanogaster CHD1 homologue (dCHD1) and its localization on polytene chromosomes...
  2. Eissenberg J, Shilatifard A, Dorokhov N, Michener D. Cdk9 is an essential kinase in Drosophila that is required for heat shock gene expression, histone methylation and elongation factor recruitment. Mol Genet Genomics. 2007;277:101-14 pubmed
    ..Levels of the chromo helicase protein CHD1 were reduced in Cdk9 knockdown chromosomes, suggesting that CHD1 is targeted to chromosomes through P-TEFb-..
  3. Marfella C, Imbalzano A. The Chd family of chromatin remodelers. Mutat Res. 2007;618:30-40 pubmed
    ..Genetic, biochemical, and structural studies demonstrate that Chd proteins are important regulators of transcription and play critical roles during developmental processes. Numerous Chd proteins are also implicated in human disease. ..
  4. Morettini S, Tribus M, Zeilner A, Sebald J, Campo Fernandez B, Scheran G, et al. The chromodomains of CHD1 are critical for enzymatic activity but less important for chromatin localization. Nucleic Acids Res. 2011;39:3103-15 pubmed publisher
    The molecular motor protein CHD1 has been implicated in the regulation of transcription and in the transcription-independent genome-wide incorporation of H3.3 into paternal chromatin in Drosophila melanogaster...
  5. McDaniel I, Lee J, Berger M, Hanagami C, Armstrong J. Investigations of CHD1 function in transcription and development of Drosophila melanogaster. Genetics. 2008;178:583-7 pubmed publisher
    In this report we describe chd1 mutant alleles and show that the CHD1 chromatin-remodeling factor is important for wing development and fertility...
  6. Podhraski V, Campo Fernandez B, Wörle H, Piatti P, Niederegger H, Böck G, et al. CenH3/CID incorporation is not dependent on the chromatin assembly factor CHD1 in Drosophila. PLoS ONE. 2010;5:e10120 pubmed publisher
    b>CHD1 is a SNF2-related ATPase that is required for the genome-wide incorporation of variant histone H3.3 in the paternal pronucleus as well as in transcriptionally active nuclei in Drosophila embryos. The S...
  7. Mueller Planitz F, Klinker H, Ludwigsen J, Becker P. The ATPase domain of ISWI is an autonomous nucleosome remodeling machine. Nat Struct Mol Biol. 2013;20:82-9 pubmed publisher
    ..Nucleosome-remodeling enzymes may thus have evolved directly from ancestral helicase-type motors, and peripheral domains have furnished regulatory capabilities that bias the remodeling reaction toward different structural outcomes. ..
  8. Loppin B, Dubruille R, Horard B. The intimate genetics of Drosophila fertilization. Open Biol. 2015;5: pubmed publisher
  9. Khattak S, Lee B, Cho S, Ahnn J, Spoerel N. Genetic characterization of Drosophila Mi-2 ATPase. Gene. 2002;293:107-14 pubmed
    ..The expression pattern of dMi-2 and the embryonic lethal phenotypes of mutants indicate that dMi-2 is essential for embryonic development in Drosophila melanagaster. ..
  10. Papantonis A, Swevers L, Iatrou K. Chorion genes: a landscape of their evolution, structure, and regulation. Annu Rev Entomol. 2015;60:177-94 pubmed publisher
  11. Emelyanov A, Rabbani J, Mehta M, Vershilova E, Keogh M, Fyodorov D. Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization. Genes Dev. 2014;28:2027-40 pubmed publisher
  12. Kim S, Bugga L, Hong E, Zabinsky R, Edwards R, Deodhar P, et al. An RNAi-Based Candidate Screen for Modifiers of the CHD1 Chromatin Remodeler and Assembly Factor in Drosophila melanogaster. G3 (Bethesda). 2015;6:245-54 pubmed publisher
    The conserved chromatin remodeling and assembly factor CHD1 (chromodomains, helicase, DNA-binding domain) is present at active genes where it participates in histone turnover and recycling during transcription...
  13. Mills A. Throwing the cancer switch: reciprocal roles of polycomb and trithorax proteins. Nat Rev Cancer. 2010;10:669-82 pubmed publisher
    ..Recent work highlights the dynamic interplay between these opposing classes of proteins, providing new avenues for understanding how these epigenetic regulators function in tumorigenesis. ..
  14. Levine M, Begun D. Evidence of spatially varying selection acting on four chromatin-remodeling loci in Drosophila melanogaster. Genetics. 2008;179:475-85 pubmed publisher
    ..Here we describe sequence variation at seven such chromatin-remodeling loci, four of which (chd1, ssrp, chm, and glu) exhibit strong differentiation between tropical and temperate populations...
  15. Eissenberg J, Lee M, Schneider J, Ilvarsonn A, Shiekhattar R, Shilatifard A. The trithorax-group gene in Drosophila little imaginal discs encodes a trimethylated histone H3 Lys4 demethylase. Nat Struct Mol Biol. 2007;14:344-6 pubmed that Lid knockdown using RNA interference results in a specific genome-wide increase in H3K4me3 levels without affecting other patterns of H3 methylation, and results in an altered distribution of the chromo-helicase protein Chd1.
  16. Orsi G, Algazeery A, Meyer R, Capri M, Sapey Triomphe L, Horard B, et al. Drosophila Yemanuclein and HIRA cooperate for de novo assembly of H3.3-containing nucleosomes in the male pronucleus. PLoS Genet. 2013;9:e1003285 pubmed publisher
    ..3 deposition pathway is not involved in paternal chromatin assembly, thus underlining the specific implication of the HIRA/YEM complex for this essential step of zygote formation. ..
  17. Bugga L, McDaniel I, Engie L, Armstrong J. The Drosophila melanogaster CHD1 chromatin remodeling factor modulates global chromosome structure and counteracts HP1a and H3K9me2. PLoS ONE. 2013;8:e59496 pubmed publisher
    b>CHD1 is a conserved chromatin remodeling factor that localizes to active genes and functions in nucleosome assembly and positioning as well as histone turnover...
  18. Brehm A, Tufteland K, Aasland R, Becker P. The many colours of chromodomains. Bioessays. 2004;26:133-40 pubmed