Cenp-C

Summary

Gene Symbol: Cenp-C
Description: Centromeric protein-C
Alias: CENP-C, CG11745, CG11746, CG31258, CLD1, CenpC, Dmel\CG31258, cenp-C, l(2)SH3 157, l(3)85Aa, l(3)SH157, centromeric protein-C, CG31258-PA, CG31258-PB, Cenp-C-PA, Cenp-C-PB, centromeric protein C, lethal (3) 85Aa, lethal (3) SH157
Species: fruit fly
Products:     Cenp-C

Top Publications

  1. Schittenhelm R, Althoff F, Heidmann S, Lehner C. Detrimental incorporation of excess Cenp-A/Cid and Cenp-C into Drosophila centromeres is prevented by limiting amounts of the bridging factor Cal1. J Cell Sci. 2010;123:3768-79 pubmed publisher
    ..We demonstrate that the low amount of endogenous Cal1 prevents centromere expansion and mitotic kinetochore failure when Cenp-A/Cid and Cenp-C are present in excess. ..
  2. Heun P, Erhardt S, Blower M, Weiss S, Skora A, Karpen G. Mislocalization of the Drosophila centromere-specific histone CID promotes formation of functional ectopic kinetochores. Dev Cell. 2006;10:303-15 pubmed
    ..Thus, CENP-A mislocalization is one possible mechanism for genome instability during cancer progression, as well as centromere plasticity during evolution. ..
  3. Schittenhelm R, Chaleckis R, Lehner C. Intrakinetochore localization and essential functional domains of Drosophila Spc105. EMBO J. 2009;28:2374-86 pubmed publisher
    ..Spc105 seems to provide a platform within the outer kinetochore allowing independent assembly of various kinetochore components. ..
  4. Schuh M, Lehner C, Heidmann S. Incorporation of Drosophila CID/CENP-A and CENP-C into centromeres during early embryonic anaphase. Curr Biol. 2007;17:237-43 pubmed
    ..Thus, our findings uncover a strikingly dynamic behavior of centromere components in anaphase. ..
  5. Pandey R, Heeger S, Lehner C. Rapid effects of acute anoxia on spindle kinetochore interactions activate the mitotic spindle checkpoint. J Cell Sci. 2007;120:2807-18 pubmed
    ..Although involving adenosine triphosphate (ATP)-consuming kinases, the spindle checkpoint can therefore be activated by spindle damage in response to acute anoxia and protect against aneuploidies. ..
  6. Raychaudhuri N, Dubruille R, Orsi G, Bagheri H, Loppin B, Lehner C. Transgenerational propagation and quantitative maintenance of paternal centromeres depends on Cid/Cenp-A presence in Drosophila sperm. PLoS Biol. 2012;10:e1001434 pubmed publisher
    ..Hence, the amount of Cid that is loaded during each cell cycle appears to be determined primarily by the preexisting centromeric Cid, with little flexibility for compensation of accidental losses...
  7. Dunleavy E, Beier N, Gorgescu W, Tang J, Costes S, Karpen G. The cell cycle timing of centromeric chromatin assembly in Drosophila meiosis is distinct from mitosis yet requires CAL1 and CENP-C. PLoS Biol. 2012;10:e1001460 pubmed publisher
  8. Mendiburo M, Padeken J, Fülöp S, Schepers A, Heun P. Drosophila CENH3 is sufficient for centromere formation. Science. 2011;334:686-90 pubmed publisher
    ..We conclude that CID is both necessary and sufficient to serve as an epigenetic centromere mark and nucleate heritable centromere function...
  9. Althoff F, Karess R, Lehner C. Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1. Mol Biol Cell. 2012;23:2275-91 pubmed publisher
    ..These findings further expand Mps1's significance for faithful mitotic chromosome segregation and emphasize the importance of its careful regulation...

More Information

Publications43

  1. Chen C, Dechassa M, Bettini E, Ledoux M, Belisario C, Heun P, et al. CAL1 is the Drosophila CENP-A assembly factor. J Cell Biol. 2014;204:313-29 pubmed publisher
    ..Collectively, this work identifies the "missing" CENP-A chaperone in flies, revealing fundamental conservation between insect and vertebrate centromere-specification mechanisms. ..
  2. Erhardt S, Mellone B, Betts C, Zhang W, Karpen G, Straight A. Genome-wide analysis reveals a cell cycle-dependent mechanism controlling centromere propagation. J Cell Biol. 2008;183:805-18 pubmed publisher
    ..Our findings identify essential components of the epigenetic machinery that ensures proper specification and propagation of the centromere and suggest a mechanism for coordinating centromere inheritance with cell division. ..
  3. Blower M, Daigle T, Kaufman T, Karpen G. Drosophila CENP-A mutations cause a BubR1-dependent early mitotic delay without normal localization of kinetochore components. PLoS Genet. 2006;2:e110 pubmed
    ..We propose that SAC components are able to monitor spindle assembly and inhibit cell cycle progression in the absence of sustained kinetochore localization. ..
  4. Orr B, Sunkel C. Drosophila CENP-C is essential for centromere identity. Chromosoma. 2011;120:83-96 pubmed publisher
    ..We suggest that CENP-C might fulfill the structural roles of the human centromere-associated proteins not identified in Drosophila. ..
  5. Przewloka M, Venkei Z, Bolanos Garcia V, Debski J, Dadlez M, Glover D. CENP-C is a structural platform for kinetochore assembly. Curr Biol. 2011;21:399-405 pubmed publisher
    Centromeres provide a region of chromatin upon which kinetochores are assembled in mitosis. Centromeric protein C (CENP-C) is a core component of this centromeric chromatin that, when depleted, prevents the proper formation of both ..
  6. Schittenhelm R, Heeger S, Althoff F, Walter A, Heidmann S, Mechtler K, et al. Spatial organization of a ubiquitous eukaryotic kinetochore protein network in Drosophila chromosomes. Chromosoma. 2007;116:385-402 pubmed
    ..The N terminus of Cenp-C is further out, clustered with Mis12 and the Spc25 end of the rod-like Ndc80 complex, which is known to bind to microtubules at its other more distal Ndc80/Nuf2 end. ..
  7. Heeger S, Leismann O, Schittenhelm R, Schraidt O, Heidmann S, Lehner C. Genetic interactions of separase regulatory subunits reveal the diverged Drosophila Cenp-C homolog. Genes Dev. 2005;19:2041-53 pubmed
    ..Characterization of its centromere localization domain revealed the presence of a diverged CENPC motif...
  8. Olszak A, van Essen D, Pereira A, Diehl S, Manke T, Maiato H, et al. Heterochromatin boundaries are hotspots for de novo kinetochore formation. Nat Cell Biol. 2011;13:799-808 pubmed publisher
    ..These data indicate that the characteristics of regions bordering heterochromatin promote de novo kinetochore assembly and thereby contribute to centromere identity. ..
  9. Mellone B, Grive K, Shteyn V, Bowers S, Oderberg I, Karpen G. Assembly of Drosophila centromeric chromatin proteins during mitosis. PLoS Genet. 2011;7:e1002068 pubmed publisher
    ..The unusual timing of CID recruitment and unique dynamics of CAL1 identify a distinct centromere assembly pathway in Drosophila and suggest that CAL1 is a key regulator of centromere propagation. ..
  10. Kuhn G. 'Satellite DNA transcripts have diverse biological roles in Drosophila'. Heredity (Edinb). 2015;115:1-2 pubmed publisher
  11. Torras Llort M, Medina Giró S, Moreno Moreno O, Azorín F. A conserved arginine-rich motif within the hypervariable N-domain of Drosophila centromeric histone H3 (CenH3) mediates BubR1 recruitment. PLoS ONE. 2010;5:e13747 pubmed publisher
  12. Ross B, Rosin L, Thomae A, Hiatt M, Vermaak D, de la Cruz A, et al. Stepwise evolution of essential centromere function in a Drosophila neogene. Science. 2013;340:1211-4 pubmed publisher
    ..Our evolutionary cell biology approach provides temporal and mechanistic detail about how young genes gain essential function. Such innovations may constantly alter the repertoire of centromeric proteins in eukaryotes...
  13. Martins T, Maia A, Steffensen S, Sunkel C. Sgt1, a co-chaperone of Hsp90 stabilizes Polo and is required for centrosome organization. EMBO J. 2009;28:234-47 pubmed publisher
    ..Taken together, these findings suggest that Sgt1 is involved in the stabilization of Polo allowing normal centrosome maturation, entry and progression though mitosis. ..
  14. Radford S, Hoang T, GÅ‚uszek A, Ohkura H, McKim K. Lateral and End-On Kinetochore Attachments Are Coordinated to Achieve Bi-orientation in Drosophila Oocytes. PLoS Genet. 2015;11:e1005605 pubmed publisher
    ..Together, these results demonstrate that, in oocytes, kinetochore-dependent and -independent chromosome-microtubule attachments work together to promote the accurate segregation of chromosomes. ..
  15. Derive N, Landmann C, Montembault E, Claverie M, Pierre Elies P, Goutte Gattat D, et al. Bub3-BubR1-dependent sequestration of Cdc20Fizzy at DNA breaks facilitates the correct segregation of broken chromosomes. J Cell Biol. 2015;211:517-32 pubmed publisher
    ..We therefore propose that the Bub3-BubR1 complex on broken DNA inhibits the APC/C locally via the sequestration of Cdc20, thus promoting proper transmission of broken chromosomes. ..
  16. Unhavaithaya Y, Orr Weaver T. Centromere proteins CENP-C and CAL1 functionally interact in meiosis for centromere clustering, pairing, and chromosome segregation. Proc Natl Acad Sci U S A. 2013;110:19878-83 pubmed publisher
    ..These results show that integral centromere proteins are required for nuclear position and intercentromere associations in meiosis. ..
  17. Lipinszki Z, Lefevre S, Savoian M, Singleton M, Glover D, Przewloka M. Centromeric binding and activity of Protein Phosphatase 4. Nat Commun. 2015;6:5894 pubmed publisher
    ..the EVH1 domain of the regulatory subunit 3 of Drosophila PP4, Falafel (Flfl), directly interacts with the centromeric protein C (CENP-C)...
  18. Rošić S, Köhler F, Erhardt S. Repetitive centromeric satellite RNA is essential for kinetochore formation and cell division. J Cell Biol. 2014;207:335-49 pubmed publisher
    ..In conclusion, our data reveal that SAT III RNA is an integral part of centromere identity, adding RNA to the complex epigenetic mark at centromeres in flies. ..
  19. Bender M, Turner F, Kaufman T. A development genetic analysis of the gene regulator of postbithorax in Drosophila melanogaster. Dev Biol. 1987;119:418-32 pubmed
    ..A possible formal relationship between Rg-pbx, ftz, and the postbithorax (pbx) locus is proposed. ..
  20. Liu Y, Petrovic A, Rombaut P, Mosalaganti S, Keller J, Raunser S, et al. Insights from the reconstitution of the divergent outer kinetochore of Drosophila melanogaster. Open Biol. 2016;6:150236 pubmed publisher
    ..All CCAN subunits, with the exception of centromeric protein C (CENP-C), and two KMN subunits, Dsn1 and Zwint, cannot be identified in this organism...
  21. Venkei Z, Przewloka M, Ladak Y, Albadri S, Sossick A, Juhasz G, et al. Spatiotemporal dynamics of Spc105 regulates the assembly of the Drosophila kinetochore. Open Biol. 2012;2:110032 pubmed publisher
    ..Our study thus indicates that physical accessibility of kinetochore components plays a crucial role in the regulation of Drosophila kinetochore assembly and leads us to a model in which Spc105 is a licensing factor for its onset. ..
  22. Bucciarelli E, Pellacani C, Naim V, Palena A, Gatti M, Somma M. Drosophila Dgt6 interacts with Ndc80, Msps/XMAP215, and gamma-tubulin to promote kinetochore-driven MT formation. Curr Biol. 2009;19:1839-45 pubmed publisher
    ..Dgt6 may also promote elongation of kinetochore-driven k-fibers through its interaction with gamma-tubulin and Msps. ..
  23. Lidsky P, Sprenger F, Lehner C. Distinct modes of centromere protein dynamics during cell cycle progression in Drosophila S2R+ cells. J Cell Sci. 2013;126:4782-93 pubmed publisher
    ..Our results reveal the complexities of Drosophila centromere protein dynamics and its intricate coordination with cell cycle progression. ..
  24. Costello J, Dalkilic M, Beason S, Gehlhausen J, Patwardhan R, Middha S, et al. Gene networks in Drosophila melanogaster: integrating experimental data to predict gene function. Genome Biol. 2009;10:R97 pubmed publisher
    ..melanogaster. The network enables the exploration, mining, and reanalysis of experimental data, as well as the interpretation of new data. The inferred annotations provide testable hypotheses of previously uncharacterized genes. ..
  25. Jones W, Rawls J. Genetic and molecular mapping of chromosome region 85A in Drosophila melanogaster. Genetics. 1988;120:733-42 pubmed
    ..The breakpoint sites of a number of 85A chromosome rearrangements were localized on the molecular map, thereby delimiting regions of the DNA that contain the various genetic complementation groups. ..
  26. Kwenda L, Collins C, Dattoli A, Dunleavy E. Nucleolar activity and CENP-C regulate CENP-A and CAL1 availability for centromere assembly in meiosis. Development. 2016;143:1400-12 pubmed publisher
    ..Finally, we show that RNA polymerase I transcription is required for efficient CENP-A assembly in meiosis, as well as centromere tethering to nucleoli. ..
  27. Liu Y, Ge Q, Chan B, Liu H, Singh S, Manley J, et al. Whole-animal genome-wide RNAi screen identifies networks regulating male germline stem cells in Drosophila. Nat Commun. 2016;7:12149 pubmed publisher
    ..We also find that mitosis/cytokinesis proteins are especially important for male GSC maintenance. Our findings provide valuable insights and resources for studying stem cell regulation at the organismal level. ..
  28. Feijão T, Afonso O, Maia A, Sunkel C. Stability of kinetochore-microtubule attachment and the role of different KMN network components in Drosophila. Cytoskeleton (Hoboken). 2013;70:661-75 pubmed publisher
  29. Fouix S, Martin Lannerée S, Sanial M, Morla L, Lamour Isnard C, Plessis A. Over-expression of a novel nuclear interactor of Suppressor of fused, the Drosophila myelodysplasia/myeloid leukaemia factor, induces abnormal morphogenesis associated with increased apoptosis and DNA synthesis. Genes Cells. 2003;8:897-911 pubmed
    ..We propose that dSu(fu) and dMLF act together at the transcriptional level to coordinate patterning and proliferation during development. ..
  30. Barth T, Schade G, Schmidt A, Vetter I, Wirth M, Heun P, et al. Identification of novel Drosophila centromere-associated proteins. Proteomics. 2014;14:2167-78 pubmed publisher
    ..All MS data have been deposited in the ProteomeXchange with identifier PXD000758 (http://proteomecentral.proteomexchange.org/dataset/PXD000758). ..
  31. Williams B, Leung G, Maiato H, Wong A, Li Z, Williams E, et al. Mitch a rapidly evolving component of the Ndc80 kinetochore complex required for correct chromosome segregation in Drosophila. J Cell Sci. 2007;120:3522-33 pubmed
    ..Despite the crucial role of Mitch in cell division, the mitch gene has evolved very rapidly among species in the genus Drosophila. ..
  32. Richter M, Poznanski J, Zdziarska A, Czarnocki Cieciura M, Lipinszki Z, Dadlez M, et al. Network of protein interactions within the Drosophila inner kinetochore. Open Biol. 2016;6:150238 pubmed publisher
    ..Thus, we conclude the organization of the Mis12-Nnf1 dimer confers upon the Mis12 complex a bipolar, elongated structure that is critical for kinetochore function. ..
  33. Unhavaithaya Y, Park E, Royzman I, Orr Weaver T. Drosophila embryonic cell-cycle mutants. G3 (Bethesda). 2013;3:1875-80 pubmed publisher
    ..Other mutations affecting the centromere protein CAL1 and the kinetochore protein Spc105R caused mitotic defects in the nervous system. ..
  34. Podhraski V, Campo Fernandez B, Wörle H, Piatti P, Niederegger H, Böck G, et al. CenH3/CID incorporation is not dependent on the chromatin assembly factor CHD1 in Drosophila. PLoS ONE. 2010;5:e10120 pubmed publisher
    ..Therefore, centromeric chromatin assembly may involve different mechanisms in different organisms. ..