Genomes and Genes
Gene Symbol: capu
Alias: CG15420, CG3399, Capu, Dmel\CG3399, capp, cappuccino, CG3399-PA, CG3399-PB, CG3399-PD, CG3399-PE, CG3399-PF, CG3399-PG, CG3399-PH, CG3399-PI, CG3399-PJ, cappucino, capu-PA, capu-PB, capu-PD, capu-PE, capu-PF, capu-PG, capu-PH, capu-PI, capu-PJ
Species: fruit fly
- Theurkauf W. Premature microtubule-dependent cytoplasmic streaming in cappuccino and spire mutant oocytes. Science. 1994;265:2093-6 pubmed..The cappuccino and spire loci are required for both posterior and dorsoventral patterning...
- Neuman Silberberg F, Schupbach T. The Drosophila dorsoventral patterning gene gurken produces a dorsally localized RNA and encodes a TGF alpha-like protein. Cell. 1993;75:165-74 pubmed..We propose that the dorsal localization of grk RNA results in a spatially restricted ligand that asymmetrically activates the receptor. ..
- Mahowald A. Germ plasm revisited and illuminated. Science. 1992;255:1216-7 pubmed
- Serbus L, Cha B, Theurkauf W, Saxton W. Dynein and the actin cytoskeleton control kinesin-driven cytoplasmic streaming in Drosophila oocytes. Development. 2005;132:3743-52 pubmed..This allows a cooperative self-amplifying loop of plus-end-directed organelle motion and parallel microtubule orientation that drives vigorous streaming currents and thorough mixing of oocyte and nurse-cell cytoplasm. ..
- Emmons S, Phan H, Calley J, Chen W, James B, Manseau L. Cappuccino, a Drosophila maternal effect gene required for polarity of the egg and embryo, is related to the vertebrate limb deformity locus. Genes Dev. 1995;9:2482-94 pubmedWe report the molecular isolation of cappuccino (capu), a gene required for localization of molecular determinants within the developing Drosophila oocyte...
- Lasko P, Ashburner M. Posterior localization of vasa protein correlates with, but is not sufficient for, pole cell development. Genes Dev. 1990;4:905-21 pubmed..These results are discussed with respect to the multiple functions of the vasa gene. ..
- Chang C, Nashchekin D, Wheatley L, Irion U, Dahlgaard K, Montague T, et al. Anterior-posterior axis specification in Drosophila oocytes: identification of novel bicoid and oskar mRNA localization factors. Genetics. 2011;188:883-96 pubmed publisher..One of the single allele suppressors proved to be a mutation in the actin nucleator, Cappuccino, revealing a previously unrecognized function of Cappuccino in pole plasm anchoring and the induction of actin ..
- Manseau L, Calley J, Phan H. Profilin is required for posterior patterning of the Drosophila oocyte. Development. 1996;122:2109-16 pubmed..and microtubule-based cytoplasmic streaming within the oocyte, similar to that which occurs prematurely in cappuccino and spire mutant oocytes...
- St Johnston D, Beuchle D, Nusslein Volhard C. Staufen, a gene required to localize maternal RNAs in the Drosophila egg. Cell. 1991;66:51-63 pubmed..By the time the egg is laid, staufen protein is also concentrated at the anterior pole, in the same region as bicoid RNA. ..
- Rosales Nieves A, Johndrow J, Keller L, Magie C, Pinto Santini D, Parkhurst S. Coordination of microtubule and microfilament dynamics by Drosophila Rho1, Spire and Cappuccino. Nat Cell Biol. 2006;8:367-76 pubmedThe actin-nucleation factors Spire and Cappuccino (Capu) regulate the onset of ooplasmic streaming in Drosophila melanogaster. Although this streaming event is microtubule-based, actin assembly is required for its timing...
- Ephrussi A, Dickinson L, Lehmann R. Oskar organizes the germ plasm and directs localization of the posterior determinant nanos. Cell. 1991;66:37-50 pubmed..We propose that the pole plasm is assembled stepwise and that continued interaction among its components is required for germ cell determination. ..
- Kim Ha J, Smith J, Macdonald P. oskar mRNA is localized to the posterior pole of the Drosophila oocyte. Cell. 1991;66:23-35 pubmed..In addition, we find that nonsense oskar mutations disrupt osk mRNA localization, while missense oskar mutations do not. ..
- Quinlan M, Heuser J, Kerkhoff E, Mullins R. Drosophila Spire is an actin nucleation factor. Nature. 2005;433:382-8 pubmed..Spire itself is conserved among metazoans and, together with the formin Cappuccino, is required for axis specification in oocytes and embryos, suggesting that multiple actin nucleation factors ..
- Shulman J, Benton R, St Johnston D. The Drosophila homolog of C. elegans PAR-1 organizes the oocyte cytoskeleton and directs oskar mRNA localization to the posterior pole. Cell. 2000;101:377-88 pubmed..These results identify a molecular parallel between anterior-posterior polarization in Drosophila and C. elegans. ..
- Clark I, Giniger E, Ruohola Baker H, Jan L, Jan Y. Transient posterior localization of a kinesin fusion protein reflects anteroposterior polarity of the Drosophila oocyte. Curr Biol. 1994;4:289-300 pubmed..The genetic requirements for this localization and its sensitivity to colchicine, both of which are shared with the posterior transport of oskar mRNA and Staufen protein, suggest that similar mechanism may function in both processes. ..
- Neuman Silberberg F. Drosophila female sterile mutation spoonbill interferes with multiple pathways in oogenesis. Genesis. 2007;45:369-81 pubmed..Based on the previous data and the results presented here, it is anticipated that spoonbill may encode a multifunctional protein that perhaps coordinately regulated the activity of multiple signaling pathways during oogenesis. ..
- McGrail M, Gepner J, Silvanovich A, Ludmann S, Serr M, Hays T. Regulation of cytoplasmic dynein function in vivo by the Drosophila Glued complex. J Cell Biol. 1995;131:411-25 pubmed..Together with the observed dependency of Glued localization on dynein function, these genetic interactions demonstrate a functional association between the Drosophila dynein motor and Glued complexes. ..
- González Reyes A, Elliott H, St Johnston D. Polarization of both major body axes in Drosophila by gurken-torpedo signalling. Nature. 1995;375:654-8 pubmed..As the gurken-torpedo/DER pathway also establishes dorsoventral polarity later in oogenesis, Drosophila uses the same germline to soma signalling pathway to determine both embryonic axes. ..
- Chen F, Barkett M, Ram K, Quintanilla A, Hariharan I. Biological characterization of Drosophila Rapgap1, a GTPase activating protein for Rap1. Proc Natl Acad Sci U S A. 1997;94:12485-90 pubmed..Thus, Rapgap1 can function as a negative regulator of Rap1-mediated signaling in vivo. ..
- St Johnston D. The intracellular localization of messenger RNAs. Cell. 1995;81:161-70 pubmed..Indeed, there are already several examples where the direct linkage between translational control and localization has been demonstrated, and these are discussed in the accompanying review by Curtis et al. (1995). ..
- Yoo H, Roth Johnson E, Bor B, Quinlan M. Drosophila Cappuccino alleles provide insight into formin mechanism and role in oogenesis. Mol Biol Cell. 2015;26:1875-86 pubmed publisherDuring Drosophila development, the formin actin nucleator Cappuccino (Capu) helps build a cytoplasmic actin mesh throughout the oocyte...
- Zeller R, Haramis A, Zuniga A, McGuigan C, Dono R, Davidson G, et al. Formin defines a large family of morphoregulatory genes and functions in establishment of the polarising region. Cell Tissue Res. 1999;296:85-93 pubmed
- Gavis E. Expeditions to the pole: RNA localization in Xenopus and Drosophila. Trends Cell Biol. 1997;7:485-92 pubmed..While specific aspects of localization differ among RNAs, similarities between pathways used by Xenopus and Drosophila suggest that common themes have been conserved among localization mechanisms. ..
- Grunert S, St Johnston D. RNA localization and the development of asymmetry during Drosophila oogenesis. Curr Opin Genet Dev. 1996;6:395-402 pubmed..Each of these symmetry-breaking steps involves the asymmetric localization of a unique structure, leading to polarization of the cytoskeleton and the localization of specific mRNAs. ..
- Nasmyth K, Jansen R. The cytoskeleton in mRNA localization and cell differentiation. Curr Opin Cell Biol. 1997;9:396-400 pubmed..Recent data imply that in Drosophila, Caenorhabditis elegans and budding yeast, proteins of the actin cytoskeleton, including unconventional myosins, play active roles in the segregation of differentiation factors and mRNAs. ..
- Lasko P. RNA sorting in Drosophila oocytes and embryos. FASEB J. 1999;13:421-33 pubmed..Prospects for filling gaps in our knowledge about the mechanisms of localizing RNAs and the importance of RNA sorting in regulating gene expression are also explored. ..
- Liu R, Abreu Blanco M, Barry K, Linardopoulou E, Osborn G, Parkhurst S. Wash functions downstream of Rho and links linear and branched actin nucleation factors. Development. 2009;136:2849-60 pubmed publisher..Wash interacts genetically with Arp2/3, and also functions downstream of Rho1 with Spire and the formin Cappuccino to control actin and microtubule dynamics during Drosophila oogenesis...
- Markussen F, Michon A, Breitwieser W, Ephrussi A. Translational control of oskar generates short OSK, the isoform that induces pole plasma assembly. Development. 1995;121:3723-32 pubmed..Finally, we show that when oskar RNA is localized, accumulation of Oskar protein requires the functions of vasa and tudor, as well as oskar itself, suggesting a positive feedback mechanism in the induction of pole plasm by oskar. ..
- Kim Ha J, Webster P, Smith J, Macdonald P. Multiple RNA regulatory elements mediate distinct steps in localization of oskar mRNA. Development. 1993;119:169-78 pubmed
- Clifford R, Schupbach T. Coordinately and differentially mutable activities of torpedo, the Drosophila melanogaster homolog of the vertebrate EGF receptor gene. Genetics. 1989;123:771-87 pubmed..Correlations observed between the various developmental defects produced by top lesions suggest that the gene possesses several differentially, though not independently, mutable activities. ..
- Smith J, Wilson J, Macdonald P. Overexpression of oskar directs ectopic activation of nanos and presumptive pole cell formation in Drosophila embryos. Cell. 1992;70:849-59 pubmed..Strikingly, formation of these ectopic pole cells is enhanced in nanos mutants. This observation may reflect competition between nanos and the germ cell determinant for a shared and limiting precursor. ..
- Lu Q, Adler P. The diaphanous gene of Drosophila interacts antagonistically with multiple wing hairs and plays a key role in wing hair morphogenesis. PLoS ONE. 2015;10:e0115623 pubmed publisher..Further we established that purified fragments of Dia and Mwh could be co-immunoprecipitated suggesting the genetic interaction could reflect a direct physical interaction. ..
- Baum B, Kunda P. Actin nucleation: spire - actin nucleator in a class of its own. Curr Biol. 2005;15:R305-8 pubmed..A recent study has now identified in Spire a third class of actin nucleator. The four short WH2 repeats within Spire bind four consecutive actin monomers to form a novel single strand nucleus for 'barbed end' actin filament elongation. ..
- Kim Ha J, Kerr K, Macdonald P. Translational regulation of oskar mRNA by bruno, an ovarian RNA-binding protein, is essential. Cell. 1995;81:403-12 pubmed..Addition of BREs to a heterologous mRNA renders it sensitive to translational repression in the ovary. ..
- Tanaka H, Takasu E, Aigaki T, Kato K, Hayashi S, Nose A. Formin3 is required for assembly of the F-actin structure that mediates tracheal fusion in Drosophila. Dev Biol. 2004;274:413-25 pubmed..These results suggested that Form3 plays a role in the F-actin assembly, which is essential for cellular rearrangement during tracheal fusion. ..
- Kirilly D, Wang S, Xie T. Self-maintained escort cells form a germline stem cell differentiation niche. Development. 2011;138:5087-97 pubmed publisher..Here, we propose that ECs form a niche that controls GSC lineage differentiation and is maintained by a non-stem cell mechanism. ..
- Stebbings H, Lane J, Talbot N. mRNA translocation and microtubules: insect ovary models. Trends Cell Biol. 1995;5:361-5 pubmed..This article explores the evidence supportive of a role for microtubules and motor proteins in these processes. ..
- Yasunaga T, Hoff S, Schell C, HelmstÃ¤dter M, Kretz O, Kuechlin S, et al. The polarity protein Inturned links NPHP4 to Daam1 to control the subapical actin network in multiciliated cells. J Cell Biol. 2015;211:963-73 pubmed publisher..Thus, Inturned appears to function as an adaptor protein that couples cilia-associated molecules to actin-modifying proteins to rearrange the local actin cytoskeleton. ..
- Shulman J, St Johnston D. Pattern formation in single cells. Trends Cell Biol. 1999;9:M60-4 pubmed..Unlike yeast, however, more complex eukaryotic cells can manifest multiple axes of polarity, suggesting that additional mechanisms have evolved to generate more elaborate patterns. ..
- Roth Johnson E, Vizcarra C, Bois J, Quinlan M. Interaction between microtubules and the Drosophila formin Cappuccino and its effect on actin assembly. J Biol Chem. 2014;289:4395-404 pubmed publisher..interactions and formin-mediated actin-microtubule cross-talk, we studied microtubule binding by Cappuccino (Capu), a formin involved in regulating actin and microtubule organization during Drosophila oogenesis...
- Ding D, Parkhurst S, Lipshitz H. Different genetic requirements for anterior RNA localization revealed by the distribution of Adducin-like transcripts during Drosophila oogenesis. Proc Natl Acad Sci U S A. 1993;90:2512-6 pubmed
- Wang C, Lehmann R. Nanos is the localized posterior determinant in Drosophila. Cell. 1991;66:637-47 pubmed..Our results demonstrate that a localized source of nos RNA is sufficient to specify abdominal segmentation and imply that other posterior group genes are required for localization, stabilization, or distribution of the nos gene product...
- Jongens T, Ackerman L, Swedlow J, Jan L, Jan Y. Germ cell-less encodes a cell type-specific nuclear pore-associated protein and functions early in the germ-cell specification pathway of Drosophila. Genes Dev. 1994;8:2123-36 pubmed..We also present evidence indicating that the gcl protein associates specifically with the nuclear pores of the pole cell nuclei. This localization suggests a novel mechanism in the specification of cell fate for the germ line. ..
- Gottlieb E. Messenger RNA transport and localization. Curr Opin Cell Biol. 1990;2:1080-6 pubmed
- Clegg N, Findley S, Mahowald A, Ruohola Baker H. Maelstrom is required to position the MTOC in stage 2-6 Drosophila oocytes. Dev Genes Evol. 2001;211:44-8 pubmed..Here we present data that are consistent with the hypothesis that maelstrom is required for posterior positioning of the MTOC. ..
- Bashirullah A, Cooperstock R, Lipshitz H. Spatial and temporal control of RNA stability. Proc Natl Acad Sci U S A. 2001;98:7025-8 pubmed..Similar mechanisms are likely to act later in development to restrict certain classes of transcripts to particular cell types within somatic cell lineages. Functions of transcript degradation and protection are discussed. ..
- Bardsley A, McDonald K, Boswell R. Distribution of tudor protein in the Drosophila embryo suggests separation of functions based on site of localization. Development. 1993;119:207-19 pubmed..Our results suggest that tudor protein localized in the germ plasm is instrumental in germ cell determination, whereas nuclear-associated tudor protein is involved in determination of segmental pattern in the abdomen. ..
- Lee S, Bagley J, Lee H, Jan L, Jan Y. Pathogenic polyglutamine proteins cause dendrite defects associated with specific actin cytoskeletal alterations in Drosophila. Proc Natl Acad Sci U S A. 2011;108:16795-800 pubmed publisher..Together, these findings suggest that specific actin cytoskeletal alterations that alter dendrite morphology and function may contribute to the pathogenesis of at least a subset of polyQ disorders, including SCA3 and SCA1. ..
- Raff J, Whitfield W, Glover D. Two distinct mechanisms localise cyclin B transcripts in syncytial Drosophila embryos. Development. 1990;110:1249-61 pubmed..The distribution pattern of the transcript at the posterior pole throughout embryogenesis and in a variety of mutant embryos suggests that this component is associated with polar granules. ..
- Lehmann R, Nusslein Volhard C. The maternal gene nanos has a central role in posterior pattern formation of the Drosophila embryo. Development. 1991;112:679-91 pubmed..We suggest that the products of these genes provide the physical structure necessary for the localization of nanos-dependent activity and of germ line determinants. ..
- Wang C, Dickinson L, Lehmann R. Genetics of nanos localization in Drosophila. Dev Dyn. 1994;199:103-15 pubmed..Localization of nanos is not affected by mutations in bicoid or torso, confirming that the three maternal systems of anterior-posterior determination initially act independently. ..
- Jackson S, Berg C. Soma-to-germline interactions during Drosophila oogenesis are influenced by dose-sensitive interactions between cut and the genes cappuccino, ovarian tumor and agnostic. Genetics. 1999;153:289-303 pubmed..Egg chambers produced by cut, cappuccino, and chickadee mutants contained binucleate cells in which ring canal remnants stained with antibodies against ..
- Neuman Silberberg F, Schupbach T. The Drosophila TGF-alpha-like protein Gurken: expression and cellular localization during Drosophila oogenesis. Mech Dev. 1996;59:105-13 pubmed..By immunoblotting we detect one major form of the Gurken protein, which likely corresponds to the unprocessed protein. ..
- Bor B, Bois J, Quinlan M. Regulation of the formin Cappuccino is critical for polarity of Drosophila oocytes. Cytoskeleton (Hoboken). 2015;72:1-15 pubmed publisherThe Drosophila formin Cappuccino (Capu) creates an actin mesh-like structure that traverses the oocyte during midoogenesis...
- Rongo C, Gavis E, Lehmann R. Localization of oskar RNA regulates oskar translation and requires Oskar protein. Development. 1995;121:2737-46 pubmed..We propose that initially localization of oskar RNA permits translation into Oskar protein and that subsequently Oskar protein regulates its own RNA localization through a positive feedback mechanism. ..
- Ephrussi A, Lehmann R. Induction of germ cell formation by oskar. Nature. 1992;358:387-92 pubmed..Of the eight genes necessary for germ cell formation at the posterior, only three, oskar, vasa and tudor, are essential at an ectopic site. ..
- Ding D, Whittaker K, Lipshitz H. Mitochondrially encoded 16S large ribosomal RNA is concentrated in the posterior polar plasm of early Drosophila embryos but is not required for pole cell formation. Dev Biol. 1994;163:503-15 pubmed..These data argue against previous hypotheses that the 16S RNA serves an obligatory function in pole cell formation. ..
- Newmark P, Mohr S, Gong L, Boswell R. mago nashi mediates the posterior follicle cell-to-oocyte signal to organize axis formation in Drosophila. Development. 1997;124:3197-207 pubmed..In the absence of mago+ function during oogenesis, the anteroposterior and dorsoventral coordinates of the oocyte are not specified and the germ plasm fails to assemble. ..
- Wilsch Bräuninger M, Schwarz H, Nusslein Volhard C. A sponge-like structure involved in the association and transport of maternal products during Drosophila oogenesis. J Cell Biol. 1997;139:817-29 pubmed..We propose that the sponge bodies are structures that, by assembly and transport of included molecules or associated structures, are involved in localization of mRNAs in Drosophila oocytes. ..
- Li M, McGrail M, Serr M, Hays T. Drosophila cytoplasmic dynein, a microtubule motor that is asymmetrically localized in the oocyte. J Cell Biol. 1994;126:1475-94 pubmed..This distribution and its disruption by specific maternal effect mutations lends support to recent models suggesting that microtubule motors participate in the transport of these morphogens from the nurse cell cytoplasm to the oocyte. ..
- Doronkin S, Djagaeva I, Beckendorf S. CSN5/Jab1 mutations affect axis formation in the Drosophila oocyte by activating a meiotic checkpoint. Development. 2002;129:5053-64 pubmed..They also reveal a link between DNA repair, axis formation and the COP9 signalosome, a protein complex that acts in multiple signaling pathways by regulating protein stability. ..
- Abreu Blanco M, Verboon J, Parkhurst S. Coordination of Rho family GTPase activities to orchestrate cytoskeleton responses during cell wound repair. Curr Biol. 2014;24:144-55 pubmed publisher..The cell wound repair response is an example of how specific pathways can be activated locally in response to the cell's needs. ..
- Bor B, Vizcarra C, Phillips M, Quinlan M. Autoinhibition of the formin Cappuccino in the absence of canonical autoinhibitory domains. Mol Biol Cell. 2012;23:3801-13 pubmed publisher..Most formins are regulated by an intramolecular interaction. The Drosophila formin, Cappuccino (Capu), was believed to be an exception...
- Kobayashi S, Amikura R, Okada M. Localization of mitochondrial large rRNA in germinal granules and the consequent segregation of germ line. Int J Dev Biol. 1994;38:193-9 pubmed
- Zhao M, Szafranski P, Hall C, Goode S. Basolateral junctions utilize warts signaling to control epithelial-mesenchymal transition and proliferation crucial for migration and invasion of Drosophila ovarian epithelial cells. Genetics. 2008;178:1947-71 pubmed publisher..Thus, the BLJ appears to regulate epithelial polarity and dynamics not only as a localized scaffold, but also by communicating signals to the nucleus. Wts may be regulated by distinct junction inputs depending on developmental context. ..
- Wang Y, Riechmann V. Microtubule anchoring by cortical actin bundles prevents streaming of the oocyte cytoplasm. Mech Dev. 2008;125:142-52 pubmed..Mutations in the actin binding proteins Profilin, Cappuccino (Capu) and Spire result in premature streaming of the cytoplasm and a reorganisation of the oocyte MT network...
- Ding D, Parkhurst S, Halsell S, Lipshitz H. Dynamic Hsp83 RNA localization during Drosophila oogenesis and embryogenesis. Mol Cell Biol. 1993;13:3773-81 pubmed..produced by females carrying maternal mutations that disrupt the posterior polar plasm and the polar granules--cappuccino, oskar, spire, staufen, tudor, valois, and vasa...
- Wilson J, Connell J, Schlenker J, Macdonald P. Novel genetic screen for genes involved in posterior body patterning in Drosophila. Dev Genet. 1996;19:199-209 pubmed..These defects are distinct from previously described perturbations in oskar activity and provide new insights into the regulation of oskar. ..
- Manseau L, Schupbach T. The egg came first, of course! Anterior-posterior pattern formation in Drosophila embryogenesis and oogenesis. Trends Genet. 1989;5:400-5 pubmed..The initial spatial localizations of the maternal organizing activities are established during oogenesis. After fertilization these activities regulate zygotic gene activity along the anterior-posterior axis of the egg. ..
- Robinson D, Cooley L. Genetic analysis of the actin cytoskeleton in the Drosophila ovary. Annu Rev Cell Dev Biol. 1997;13:147-70 pubmed..We discuss many of the recent genetic and cell biological studies that have led to insights into how the actin cytoskeleton is assembled and regulated during the morphogenesis of the Drosophila egg. ..
- Serano T, Cohen R. Gratuitous mRNA localization in the Drosophila oocyte. Development. 1995;121:3013-21 pubmed..We show that mutations in cappuccino and spire, which permit K10 mRNA transport, but prevent subsequent anterior localization, do not disrupt the ..
- Jongens T, Hay B, Jan L, Jan Y. The germ cell-less gene product: a posteriorly localized component necessary for germ cell development in Drosophila. Cell. 1992;70:569-84 pubmed..Consistent with this phenotype, gcl protein specifically associates with those nuclei that later become the nuclei of the germ cell precursors. These observations suggest that gcl functions in the germ cell specification pathway. ..