Gene Symbol: cal1
Description: chromosome alignment defect 1
Alias: CAL1, CG5148, CLD2, Cal1, Dmel\CG5148, chromosome alignment defect 1, CG5148-PA, cal1-PA
Species: fruit fly

Top Publications

  1. Chen C, GREENE E, Bowers S, Mellone B. A role for the CAL1-partner Modulo in centromere integrity and accurate chromosome segregation in Drosophila. PLoS ONE. 2012;7:e45094 pubmed publisher
    ..Here we identify the nucleolar protein, Modulo, in complex with CAL1, a factor essential for the centromeric deposition of the centromere-specific histone H3 variant, CID, in ..
  2. Raychaudhuri N, Dubruille R, Orsi G, Bagheri H, Loppin B, Lehner C. Transgenerational propagation and quantitative maintenance of paternal centromeres depends on Cid/Cenp-A presence in Drosophila sperm. PLoS Biol. 2012;10:e1001434 pubmed publisher
    ..Hence, the amount of Cid that is loaded during each cell cycle appears to be determined primarily by the preexisting centromeric Cid, with little flexibility for compensation of accidental losses...
  3. Erhardt S, Mellone B, Betts C, Zhang W, Karpen G, Straight A. Genome-wide analysis reveals a cell cycle-dependent mechanism controlling centromere propagation. J Cell Biol. 2008;183:805-18 pubmed publisher
    ..We identified the proteins CAL1 and CENP-C as essential factors for CID assembly at the centromere...
  4. Schittenhelm R, Althoff F, Heidmann S, Lehner C. Detrimental incorporation of excess Cenp-A/Cid and Cenp-C into Drosophila centromeres is prevented by limiting amounts of the bridging factor Cal1. J Cell Sci. 2010;123:3768-79 pubmed publisher
    ..With yeast three-hybrid experiments, we demonstrate that the essential Drosophila centromere protein Cal1 can link Cenp-A/Cid and Cenp-C...
  5. Mellone B, Grive K, Shteyn V, Bowers S, Oderberg I, Karpen G. Assembly of Drosophila centromeric chromatin proteins during mitosis. PLoS Genet. 2011;7:e1002068 pubmed publisher
    ..chromatin assembly in Drosophila, which contains the H3 variant CID (CENP-A in humans), as well as CENP-C and CAL1, which are required for CID localization...
  6. Dunleavy E, Beier N, Gorgescu W, Tang J, Costes S, Karpen G. The cell cycle timing of centromeric chromatin assembly in Drosophila meiosis is distinct from mitosis yet requires CAL1 and CENP-C. PLoS Biol. 2012;10:e1001460 pubmed publisher
    ..Finally, we show that the centromere proteins CAL1 and CENP-C are both required for CID assembly in meiosis and normal progression through spermatogenesis...
  7. Chen C, Dechassa M, Bettini E, Ledoux M, Belisario C, Heun P, et al. CAL1 is the Drosophila CENP-A assembly factor. J Cell Biol. 2014;204:313-29 pubmed publisher
    ..In Drosophila, centromeric deposition of CENP-A requires the fly-specific protein CAL1. Here, we show that targeting CAL1 to noncentromeric DNA in Drosophila cells is sufficient to heritably recruit ..
  8. Phansalkar R, Lapierre P, Mellone B. Evolutionary insights into the role of the essential centromere protein CAL1 in Drosophila. Chromosome Res. 2012;20:493-504 pubmed publisher
    ..How CENP-A is targeted to centromeres in these organisms is not known. The Drosophila centromeric protein CAL1, found only in the Diptera genus, is essential for CENP-A localization, is recruited to centromeres at a similar ..
  9. Boltengagen M, Huang A, Boltengagen A, Trixl L, Lindner H, Kremser L, et al. A novel role for the histone acetyltransferase Hat1 in the CENP-A/CID assembly pathway in Drosophila melanogaster. Nucleic Acids Res. 2016;44:2145-59 pubmed publisher
    ..Two complexes contain the CENP-A chaperones CAL1, FACT and/or Caf1/Rbap48...

More Information


  1. Chen C, Bowers S, Lipinszki Z, Palladino J, Trusiak S, Bettini E, et al. Establishment of Centromeric Chromatin by the CENP-A Assembly Factor CAL1 Requires FACT-Mediated Transcription. Dev Cell. 2015;34:73-84 pubmed publisher
    ..centromere function and cell-cycle progression, we demonstrate that CENP-A assembly by its loading factor, CAL1, requires RNAPII-mediated transcription of the underlying DNA...
  2. Barth T, Schade G, Schmidt A, Vetter I, Wirth M, Heun P, et al. Identification of novel Drosophila centromere-associated proteins. Proteomics. 2014;14:2167-78 pubmed publisher
    ..All MS data have been deposited in the ProteomeXchange with identifier PXD000758 ( ..
  3. Bade D, Pauleau A, Wendler A, Erhardt S. The E3 ligase CUL3/RDX controls centromere maintenance by ubiquitylating and stabilizing CENP-A in a CAL1-dependent manner. Dev Cell. 2014;28:508-19 pubmed publisher
    ..CENP-A deposition to centromeres depends on a specialized loading factor from yeast to humans that is called CAL1 in Drosophila. Here, we show that CAL1 directly interacts with RDX, an adaptor for CUL3-mediated ubiquitylation...
  4. Unhavaithaya Y, Orr Weaver T. Centromere proteins CENP-C and CAL1 functionally interact in meiosis for centromere clustering, pairing, and chromosome segregation. Proc Natl Acad Sci U S A. 2013;110:19878-83 pubmed publisher
    ..exploited Drosophila mutants for the centromere proteins centromere protein-C (CENP-C) and chromosome alignment 1 (CAL1) to demonstrate that a functional centromere is needed for centromere clustering and pairing...
  5. Unhavaithaya Y, Park E, Royzman I, Orr Weaver T. Drosophila embryonic cell-cycle mutants. G3 (Bethesda). 2013;3:1875-80 pubmed publisher
    ..Other mutations affecting the centromere protein CAL1 and the kinetochore protein Spc105R caused mitotic defects in the nervous system.
  6. Rosin L, Mellone B. Co-evolving CENP-A and CAL1 Domains Mediate Centromeric CENP-A Deposition across Drosophila Species. Dev Cell. 2016;37:136-47 pubmed publisher
    ..We show that this result is due to the inability of the mel CENP-A chaperone, CAL1, to deposit bip CENP-A into chromatin...
  7. Kwenda L, Collins C, Dattoli A, Dunleavy E. Nucleolar activity and CENP-C regulate CENP-A and CAL1 availability for centromere assembly in meiosis. Development. 2016;143:1400-12 pubmed publisher
    ..In Drosophila males we show that the centromere assembly factors CAL1 and CENP-C are required for meiotic chromosome segregation, CENP-A assembly and maintenance on sperm, as well as ..
  8. Lidsky P, Sprenger F, Lehner C. Distinct modes of centromere protein dynamics during cell cycle progression in Drosophila S2R+ cells. J Cell Sci. 2013;126:4782-93 pubmed publisher
    ..Thus we have analyzed the dynamics of the three key players Cid/Cenp-A, Cenp-C and Cal1 in S2R+ cells using quantitative microscopy and fluorescence recovery after photobleaching, in combination with ..
  9. Venkei Z, Przewloka M, Glover D. Drosophila Mis12 complex acts as a single functional unit essential for anaphase chromosome movement and a robust spindle assembly checkpoint. Genetics. 2011;187:131-40 pubmed publisher
    ..Importantly our results indicate that core structural elements of the inner plate of the kinetochore have a greater contribution to faithful chromosome segregation in anaphase than in earlier stages of mitosis. ..
  10. Wright A, Fox A, Johnson K, Zinn K. Systematic screening of Drosophila deficiency mutations for embryonic phenotypes and orphan receptor ligands. PLoS ONE. 2010;5:e12288 pubmed publisher
    ..In the future, these deficiency kits will allow Drosophila researchers to rapidly and efficiently execute genome-wide anatomical screens that require examination of individual embryos at high magnification. ..