Gene Symbol: Buffy
Description: buffy
Alias: BUFFY, Bcl-2-48AE, Buffy/Borg2, Buffy/DBorg-2, Buffy/dBorg-2, CG8238, Dbx, Dmel\CG8238, Drob2, buffy, dBORG-2/BUFFY, dBorg-2, dBorg2, dbuffy, buffy, 48A-E Bcl-2-like, Buffy-PA, CG8238-PA
Species: fruit fly

Top Publications

  1. Colussi P, Quinn L, Huang D, Coombe M, Read S, Richardson H, et al. Debcl, a proapoptotic Bcl-2 homologue, is a component of the Drosophila melanogaster cell death machinery. J Cell Biol. 2000;148:703-14 pubmed
    ..RNA interference studies indicate that Debcl is required for developmental apoptosis in Drosophila embryos. These results suggest that the main components of the mammalian apoptosis machinery are conserved in insects. ..
  2. Kornbluth S, White K. Apoptosis in Drosophila: neither fish nor fowl (nor man, nor worm). J Cell Sci. 2005;118:1779-87 pubmed
    ..Thus, although Drosophila, worms and humans share a host of apoptotic regulators, the way in which they function may not be identical. ..
  3. Brachmann C, Jassim O, Wachsmuth B, Cagan R. The Drosophila bcl-2 family member dBorg-1 functions in the apoptotic response to UV-irradiation. Curr Biol. 2000;10:547-50 pubmed
    ..Finally, ectopic expression of dBorg-1 in the eye led to subtle defects that were strongly potentiated by ultra violet (UV) irradiation, resulting in a dramatic loss of retinal cells. ..
  4. Zhang H, Holzgreve W, de Geyter C. Evolutionarily conserved Bok proteins in the Bcl-2 family. FEBS Lett. 2000;480:311-3 pubmed
  5. Senoo Matsuda N, Igaki T, Miura M. Bax-like protein Drob-1 protects neurons from expanded polyglutamine-induced toxicity in Drosophila. EMBO J. 2005;24:2700-13 pubmed
    ..Either the RNAi-mediated downregulation of Drob-1 or overexpression of Drob-1 antagonist Buffy strongly enhances the polyglutamine-induced accumulation of ubiquitinated proteins and subsequent ..
  6. Sevrioukov E, Burr J, Huang E, Assi H, Monserrate J, Purves D, et al. Drosophila Bcl-2 proteins participate in stress-induced apoptosis, but are not required for normal development. Genesis. 2007;45:184-93 pubmed
    ..Two bcl-2 genes are encoded in the Drosophila genome (debcl/dBorg1/Drob-1/dBok and buffy/dBorg2) and previous RNAi-based studies suggested a requirement for these in embryonic development...
  7. Tanner E, Blute T, Brachmann C, McCall K. Bcl-2 proteins and autophagy regulate mitochondrial dynamics during programmed cell death in the Drosophila ovary. Development. 2011;138:327-38 pubmed publisher
    ..Furthermore, Bcl-2 family mutants show a striking defect in cell death in the ovary. These data indicate that a mitochondrial pathway is a major mechanism for activation of cell death in Drosophila oogenesis. ..
  8. Wu J, Nguyen N, Aghazarian M, Tan Y, Sevrioukov E, Mabuchi M, et al. grim promotes programmed cell death of Drosophila microchaete glial cells. Mech Dev. 2010;127:407-17 pubmed publisher
    ..to activate a mitochondrial death pathway distinct from IAP antagonization; therefore, the Drosophila bcl-2 genes buffy and debcl were investigated for genetic interaction with grim...
  9. Doumanis J, Dorstyn L, Kumar S. Molecular determinants of the subcellular localization of the Drosophila Bcl-2 homologues DEBCL and BUFFY. Cell Death Differ. 2007;14:907-15 pubmed
    ..In Drosophila, both Bcl-2 family members, DEBCL and BUFFY, contain an MA...

More Information


  1. Igaki T, Miura M. Role of Bcl-2 family members in invertebrates. Biochim Biophys Acta. 2004;1644:73-81 pubmed
    ..On the other hand, the Drosophila genome encodes two Bcl-2 family proteins, Drob-1/Debcl/dBorg-1/dBok and Buffy/dBorg-2, both of which structurally belong to the pro-apoptotic group, despite abundant similarities in the cell ..
  2. Quinn L, Coombe M, Mills K, Daish T, Colussi P, Kumar S, et al. Buffy, a Drosophila Bcl-2 protein, has anti-apoptotic and cell cycle inhibitory functions. EMBO J. 2003;22:3568-79 pubmed
    ..cells, but only the pro-apoptotic protein Debcl has been characterized in Drosophila: Here we report that Buffy, the second Drosophila Bcl-2-like protein, is a pro-survival protein...
  3. Sakamaki K, Imai K, Tomii K, Miller D. Evolutionary analyses of caspase-8 and its paralogs: Deep origins of the apoptotic signaling pathways. Bioessays. 2015;37:767-76 pubmed publisher
    ..The apoptotic network of the eumetazoan ancestor appears to have been complex and vertebrate like, and is only now being revealed by studying simple animals. ..
  4. Lanave C, Santamaria M, Saccone C. Comparative genomics: the evolutionary history of the Bcl-2 family. Gene. 2004;333:71-9 pubmed
    ..Once the functions of all the components are known, it will be possible to study the process in a holistic way. ..
  5. M Angale P, Staveley B. Loss of porin function in dopaminergic neurons of Drosophila is suppressed by Buffy. J Biomed Sci. 2016;23:84 pubmed
    ..In addition, the porin-induced phenotypes are greatly suppressed when the pro-survival Bcl-2 homologue Buffy is overexpressed in these neurons and in the developing eye adding to the cellular advantages of altered ..
  6. Wu Y, Zhuang Y, Han M, Xu T, Deng K. Ras promotes cell survival by antagonizing both JNK and Hid signals in the Drosophila eye. BMC Dev Biol. 2009;9:53 pubmed publisher
    ..Thus, in addition to its requirement for cell viability, Ras appears to mediate different biological roles depending on the developmental context and on the level of its expression. ..
  7. Lawen A. Apoptosis-an introduction. Bioessays. 2003;25:888-96 pubmed
  8. M Angale P, Staveley B. Overexpression of Buffy enhances the loss of parkin and suppresses the loss of Pink1 phenotypes in Drosophila. Genome. 2017;60:241-247 pubmed publisher
    ..To further evaluate the effects of the overexpression of the Bcl-2 homologue Buffy, we analysed lifespan and climbing ability in both parkin-RNAi- and Pink1-RNAi-expressing flies...
  9. Mast J, Tomalty K, Vogel H, Clandinin T. Reactive oxygen species act remotely to cause synapse loss in a Drosophila model of developmental mitochondrial encephalopathy. Development. 2008;135:2669-79 pubmed publisher
    ..Thus, alterations in electron transport chain function explain many of the neurodegenerative changes seen in both early- and late-onset disorders. ..
  10. Kwon D, Mucci D, Langlais K, Americo J, DeVido S, Cheng Y, et al. Enhancer-promoter communication at the Drosophila engrailed locus. Development. 2009;136:3067-75 pubmed publisher
    ..These data show that multiple mechanisms ensure proper enhancer-promoter communication at the Drosophila engrailed locus. ..
  11. Park J, Lee S, Lee S, Kim Y, Song S, Kim S, et al. Mitochondrial dysfunction in Drosophila PINK1 mutants is complemented by parkin. Nature. 2006;441:1157-61 pubmed
    ..Taken together, our genetic evidence clearly establishes that Parkin and PINK1 act in a common pathway in maintaining mitochondrial integrity and function in both muscles and dopaminergic neurons. ..
  12. Nakazawa M, Matsubara H, Matsushita Y, Watanabe M, Vo N, Yoshida H, et al. The Human Bcl-2 Family Member Bcl-rambo Localizes to Mitochondria and Induces Apoptosis and Morphological Aberrations in Drosophila. PLoS ONE. 2016;11:e0157823 pubmed publisher
    ..The results of the present study demonstrated that human Bcl-rambo localized to mitochondria and at least regulated an apoptosis signaling pathway in Drosophila. ..
  13. Igaki T, Yamamoto Goto Y, Tokushige N, Kanda H, Miura M. Down-regulation of DIAP1 triggers a novel Drosophila cell death pathway mediated by Dark and DRONC. J Biol Chem. 2002;277:23103-6 pubmed
    ..These results suggest the existence of a novel mechanism for cell death signaling in Drosophila that is mediated by DRONC and Dark. ..
  14. Mensah L, Davison C, Fan S, Morris J, Goberdhan D, Wilson C. Fine-Tuning of PI3K/AKT Signalling by the Tumour Suppressor PTEN Is Required for Maintenance of Flight Muscle Function and Mitochondrial Integrity in Ageing Adult Drosophila melanogaster. PLoS ONE. 2015;10:e0143818 pubmed publisher
    ..We show that the phenotype is partially rescued by muscle-specific expression of the Bcl-2 homologue Buffy, which in flies, maintains mitochondrial integrity, modulates energy homeostasis and suppresses cell death...
  15. Clavier A, Baillet A, Rincheval Arnold A, Coléno Costes A, Lasbleiz C, Mignotte B, et al. The pro-apoptotic activity of Drosophila Rbf1 involves dE2F2-dependent downregulation of diap1 and buffy mRNA. Cell Death Dis. 2014;5:e1405 pubmed publisher
    ..Furthermore, we highlight that Rbf1 and dE2F2 downregulate two major anti-apoptotic genes in Drosophila: buffy, an anti-apoptotic member of Bcl-2 family and diap1, a gene encoding a caspase inhibitor...
  16. Cao C, Liu Y, Lehmann M. Fork head controls the timing and tissue selectivity of steroid-induced developmental cell death. J Cell Biol. 2007;176:843-52 pubmed
    ..More generally, they suggest that cell identity factors like Fkh play a pivotal role in the normal control of developmental cell death. ..
  17. Terhzaz S, Finlayson A, Stirrat L, Yang J, Tricoire H, Woods D, et al. Cell-specific inositol 1,4,5 trisphosphate 3-kinase mediates epithelial cell apoptosis in response to oxidative stress in Drosophila. Cell Signal. 2010;22:737-48 pubmed publisher
    ..The Drosophila orthologues of human apoptotic bcl-2 genes include debcl and buffy. Oxidative stress challenge does not modulate gene expression of either debcl or buffy in tubules; and altered ..
  18. M Angale P, Staveley B. The Bcl-2 homologue Buffy rescues ?-synuclein-induced Parkinson disease-like phenotypes in Drosophila. BMC Neurosci. 2016;17:24 pubmed publisher
    ..genes have been found in Drosophila melanogaster including the pro-cell survival, human Bok-related orthologue, Buffy. The directed expression of ?-synuclein, the first gene identified to contribute to inherited forms of Parkinson ..
  19. Clavier A, Ruby V, Rincheval Arnold A, Mignotte B, Guénal I. The Drosophila retinoblastoma protein, Rbf1, induces a Debcl- and Drp1-dependent mitochondrial apoptosis. J Cell Sci. 2015;128:3239-49 pubmed publisher
    ..activity depends on its ability to decrease the level of anti-apoptotic proteins such as the Bcl-2 family protein Buffy. Buffy often acts in an opposite manner to Debcl, the other Drosophila Bcl-2-family protein...
  20. Denton D, Kumar S. Studying Apoptosis in Drosophila. Cold Spring Harb Protoc. 2015;2015:609-13 pubmed publisher
    ..The focus of this discussion is to introduce methodologies for monitoring apoptosis during Drosophila development and also in Drosophila cell lines. ..
  21. Arya R, White K. Cell death in development: Signaling pathways and core mechanisms. Semin Cell Dev Biol. 2015;39:12-9 pubmed publisher
    ..Understanding how the cell death is regulated during development may provide insight into how this process can be manipulated in the treatment of disease. ..
  22. M Angale P, Staveley B. A loss of Pdxk model of Parkinson disease in Drosophila can be suppressed by Buffy. BMC Res Notes. 2017;10:205 pubmed publisher
    ..We relied on the pro-survival functions of Buffy, an anti-apoptotic Bcl-2 homologue, to rescue the Pdxk-induced phenotypes...
  23. Rallis A, Lu B, Ng J. Molecular chaperones protect against JNK- and Nmnat-regulated axon degeneration in Drosophila. J Cell Sci. 2013;126:838-49 pubmed publisher
    ..These results suggest that molecular chaperones are key in JNK- and Nmnat-regulated axonal protective functions. ..
  24. Monserrate J, Chen M, Brachmann C. Drosophila larvae lacking the bcl-2 gene, buffy, are sensitive to nutrient stress, maintain increased basal target of rapamycin (Tor) signaling and exhibit characteristics of altered basal energy metabolism. BMC Biol. 2012;10:63 pubmed publisher
    ..We report that starvation of Drosophila larvae lacking the bcl-2 gene, buffy, decreases survival rate by more than twofold relative to wild-type larvae...
  25. ZIRIN J, Perrimon N. Drosophila as a model system to study autophagy. Semin Immunopathol. 2010;32:363-72 pubmed publisher
    ..Furthermore, the complex biology and physiology of Drosophila presents an opportunity to model human diseases in a tissue specific and analogous context. ..
  26. Danial N, Korsmeyer S. Cell death: critical control points. Cell. 2004;116:205-19 pubmed
    ..The identification of critical control points in the cell death pathway has yielded fundamental insights for basic biology, as well as provided rational targets for new therapeutics. ..
  27. Pirooznia S, Sarthi J, Johnson A, Toth M, Chiu K, Koduri S, et al. Tip60 HAT activity mediates APP induced lethality and apoptotic cell death in the CNS of a Drosophila Alzheimer's disease model. PLoS ONE. 2012;7:e41776 pubmed publisher
  28. Lam D, Shah S, de Castro I, Loh S, Martins L. Drosophila happyhour modulates JNK-dependent apoptosis. Cell Death Dis. 2010;1:e66 pubmed publisher
    ..Together, our results show that hppy has a JNK-dependent proapoptotic function in Drosophila, which reinforces the hypothesis that MAP4K3 might act as tumour suppressor by regulating apoptosis in higher eukaryotes. ..
  29. Tsubouchi A, Tsuyama T, Fujioka M, Kohda H, Okamoto Furuta K, Aigaki T, et al. Mitochondrial protein Preli-like is required for development of dendritic arbors and prevents their regression in the Drosophila sensory nervous system. Development. 2009;136:3757-66 pubmed publisher
    ..Moreover, expression of the Drob-1 antagonist Buffy in prel mutant neurons substantially restored the dendritic phenotype...
  30. Meier P, Finch A, Evan G. Apoptosis in development. Nature. 2000;407:796-801 pubmed
    ..Dysfunctions in the regulation or execution of cell suicide are implicated in a wide range of developmental abnormalities and diseases. ..