asl

Summary

Gene Symbol: asl
Description: asterless
Alias: ASL, Asl, CG2919, Dmel\CG2919, mecD, sdu, asterless, CG2919-PA, asl-PA, mechanosensory D, mechanosensory mutant D, star dust
Species: fruit fly

Top Publications

  1. Brodu V, Baffet A, Le Droguen P, Casanova J, Guichet A. A developmentally regulated two-step process generates a noncentrosomal microtubule network in Drosophila tracheal cells. Dev Cell. 2010;18:790-801 pubmed publisher
    ..We further show that these changes are essential for tracheal development, thus stressing the functional relevance of MT reorganization for morphogenesis. ..
  2. Rebollo E, Sampaio P, Januschke J, Llamazares S, Varmark H, Gonzalez C. Functionally unequal centrosomes drive spindle orientation in asymmetrically dividing Drosophila neural stem cells. Dev Cell. 2007;12:467-74 pubmed
    ..Apical aster maintenance requires the function of Pins. These results reveal that spindle orientation in Drosophila larval NBs is determined very early in the cell cycle, and is mediated by asymmetric centrosome function. ..
  3. Stevens N, Dobbelaere J, Brunk K, Franz A, Raff J. Drosophila Ana2 is a conserved centriole duplication factor. J Cell Biol. 2010;188:313-23 pubmed publisher
    ..Here, we find that of eight candidate duplication factors identified in cultured fly cells, only two, Ana2 and Asterless (Asl), share this ability...
  4. Rusan N, Peifer M. A role for a novel centrosome cycle in asymmetric cell division. J Cell Biol. 2007;177:13-20 pubmed
    ..We propose a two-step mechanism to ensure faithful spindle positioning: the novel centrosome cycle produces a single interphase MTOC, coarsely aligning the spindle, and spindle-cortex interactions refine this alignment...
  5. Januschke J, Gonzalez C. The interphase microtubule aster is a determinant of asymmetric division orientation in Drosophila neuroblasts. J Cell Biol. 2010;188:693-706 pubmed publisher
    ..We have also found that the span of such memory is limited to the last mitosis. Furthermore, the orientation of the NB axis of polarity can be reset to any angle with respect to the surrounding tissue and is, therefore, cell autonomous...
  6. Nguyen M, Stone M, Rolls M. Microtubules are organized independently of the centrosome in Drosophila neurons. Neural Dev. 2011;6:38 pubmed publisher
    ..We conclude that the centrosome is not a major site of microtubule nucleation in Drosophila neurons, and is not required for maintenance of neuronal microtubule organization in these cells. ..
  7. Castellanos E, Dominguez P, Gonzalez C. Centrosome dysfunction in Drosophila neural stem cells causes tumors that are not due to genome instability. Curr Biol. 2008;18:1209-14 pubmed publisher
    ..We propose that such tumors might be caused by impaired asymmetric division of neural stem cells [4]. These results show that centrosome loss, far from being innocuous, is a potentially dangerous condition in flies...
  8. Januschke J, Llamazares S, Reina J, Gonzalez C. Drosophila neuroblasts retain the daughter centrosome. Nat Commun. 2011;2:243 pubmed publisher
    ..Our results demonstrate maturation-dependent centrosome fate in Drosophila NBs and that the stemness properties of these cells are not linked to mother centrosome inheritance. ..
  9. Enjolras C, Thomas J, Chhin B, Cortier E, Duteyrat J, Soulavie F, et al. Drosophila chibby is required for basal body formation and ciliogenesis but not for Wg signaling. J Cell Biol. 2012;197:313-25 pubmed publisher
    ..The function of CBY in WNT signaling in vertebrates has either been acquired during vertebrate evolution or lost in Drosophila. ..

More Information

Publications74

  1. Novak Z, Conduit P, Wainman A, Raff J. Asterless licenses daughter centrioles to duplicate for the first time in Drosophila embryos. Curr Biol. 2014;24:1276-82 pubmed publisher
    ..In flies and humans, the Asterless/Cep152 protein interacts with Sak/Plk4 and Sas-4/CPAP and is required for centriole duplication, although its ..
  2. Giansanti M, Gatti M, Bonaccorsi S. The role of centrosomes and astral microtubules during asymmetric division of Drosophila neuroblasts. Development. 2001;128:1137-45 pubmed
    ..We examined neuroblast division in asterless (asl) mutants, which, although devoid of functional centrosomes and astral microtubules, form well-focused ..
  3. Conduit P, Brunk K, Dobbelaere J, Dix C, Lucas E, Raff J. Centrioles regulate centrosome size by controlling the rate of Cnn incorporation into the PCM. Curr Biol. 2010;20:2178-86 pubmed publisher
    ..Cnn incorporation into the PCM is driven by an interaction with the conserved centriolar proteins Asl (Cep152 in humans) and DSpd-2 (Cep192 in humans)...
  4. Cunha Ferreira I, Bento I, Pimenta Marques A, Jana S, Lince Faria M, Duarte P, et al. Regulation of autophosphorylation controls PLK4 self-destruction and centriole number. Curr Biol. 2013;23:2245-2254 pubmed publisher
    ..As ?TrCP, PLK4, and centriole number are deregulated in several cancers, our work provides novel links between centriole number control and tumorigenesis. ..
  5. Varmark H, Llamazares S, Rebollo E, Lange B, Reina J, Schwarz H, et al. Asterless is a centriolar protein required for centrosome function and embryo development in Drosophila. Curr Biol. 2007;17:1735-45 pubmed
    ..We have cloned asterless (asl) and found that it encodes a 120 kD highly coiled-coil protein that is a constitutive pancentriolar and ..
  6. Giansanti M, Bucciarelli E, Bonaccorsi S, Gatti M. Drosophila SPD-2 is an essential centriole component required for PCM recruitment and astral-microtubule nucleation. Curr Biol. 2008;18:303-9 pubmed publisher
    ..We found that DSpd-2 localization at the centrosome requires the wild-type activity of Asl but is independent of the function of D-PLP, Cnn, gamma-tubulin, DGrip91, and D-TACC...
  7. Sabino D, Brown N, Basto R. Drosophila Ajuba is not an Aurora-A activator but is required to maintain Aurora-A at the centrosome. J Cell Sci. 2011;124:1156-66 pubmed publisher
    ..On the basis of our studies in Drosophila neuroblasts, we propose that a key function of Ajuba, in these cells, is to maintain active Aur-A at the centrosome during mitosis. ..
  8. Stevens N, Roque H, Raff J. DSas-6 and Ana2 coassemble into tubules to promote centriole duplication and engagement. Dev Cell. 2010;19:913-9 pubmed publisher
    ..We conclude that DSas-6 and Ana2 normally cooperate to drive the formation of the centriole inner cartwheel and that they promote both centriole duplication and centriole cohesion in a Sak/Plk4-dependent manner...
  9. Fu J, Glover D. Structured illumination of the interface between centriole and peri-centriolar material. Open Biol. 2012;2:120104 pubmed publisher
    ..microtubules, Sas-4, Spd-2 and Polo kinase; and an outer layer comprising Pericentrin-like protein (Dplp), Asterless (Asl) and Plk4 kinase...
  10. Januschke J, Reina J, Llamazares S, Bertran T, Rossi F, Roig J, et al. Centrobin controls mother-daughter centriole asymmetry in Drosophila neuroblasts. Nat Cell Biol. 2013;15:241-8 pubmed publisher
    ..CNB co-precipitates with a set of centrosomal proteins that include γ-TUB, ANA2, CNN, SAS-4, ASL, DGRIP71, POLO and SAS-6...
  11. Blachon S, Cai X, Roberts K, Yang K, Polyanovsky A, Church A, et al. A proximal centriole-like structure is present in Drosophila spermatids and can serve as a model to study centriole duplication. Genetics. 2009;182:133-44 pubmed publisher
    ..We propose that, during the evolution of insects, the proximal centriole was simplified by eliminating the later steps in centriole assembly. The PCL may provide a unique model to study early steps of centriole formation. ..
  12. Blachon S, Gopalakrishnan J, Omori Y, Polyanovsky A, Church A, Nicastro D, et al. Drosophila asterless and vertebrate Cep152 Are orthologs essential for centriole duplication. Genetics. 2008;180:2081-94 pubmed publisher
    ..Asl was previously reported to be a centrosomal protein essential for centrosome function. Here we identify mecD, a severe loss-of-function allele of the asl gene, and demonstrate that it is required for centriole and cilia ..
  13. Gopalakrishnan J, Mennella V, Blachon S, Zhai B, Smith A, Megraw T, et al. Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome. Nat Commun. 2011;2:359 pubmed publisher
    ..In this study, we show that Sas-4 provides a scaffold for cytoplasmic complexes (named S-CAP), which include CNN, Asl and D-PLP, proteins that are all found in the centrosomes at the vicinity of the centriole...
  14. Dix C, Raff J. Drosophila Spd-2 recruits PCM to the sperm centriole, but is dispensable for centriole duplication. Curr Biol. 2007;17:1759-64 pubmed
    ..We speculate that the SPD-2 family of proteins might only be absolutely essential for the recruitment of centriole duplication factors and PCM to the centriole(s) that enter the egg with the fertilizing sperm...
  15. Dzhindzhev N, Yu Q, Weiskopf K, Tzolovsky G, Cunha Ferreira I, Riparbelli M, et al. Asterless is a scaffold for the onset of centriole assembly. Nature. 2010;467:714-8 pubmed publisher
    ..Here we show that the centriolar protein Asterless (Asl; human orthologue CEP152) provides a conserved molecular platform, the amino terminus of which interacts ..
  16. Bonaccorsi S, Giansanti M, Gatti M. Spindle assembly in Drosophila neuroblasts and ganglion mother cells. Nat Cell Biol. 2000;2:54-6 pubmed
  17. Roque H, Wainman A, Richens J, Kozyrska K, Franz A, Raff J. Drosophila Cep135/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation. J Cell Sci. 2012;125:5881-6 pubmed publisher
    ..illumination microscopy we show that Cep135/Bld10 is localized to a region between inner (SAS-6, Ana2) and outer (Asl, DSpd-2 and D-PLP) centriolar components, and the localization of all these component is subtly perturbed in the ..
  18. Bonaccorsi S, Giansanti M, Gatti M. Spindle self-organization and cytokinesis during male meiosis in asterless mutants of Drosophila melanogaster. J Cell Biol. 1998;142:751-61 pubmed
    ..We have identified a gene we call asterless (asl) that is required for aster formation during male meiosis...
  19. Riparbelli M, Gottardo M, Glover D, Callaini G. Inhibition of Polo kinase by BI2536 affects centriole separation during Drosophila male meiosis. Cell Cycle. 2014;13:2064-72 pubmed publisher
    ..Despite these defects spermatid differentiation proceeds, leading to axoneme formation. ..
  20. Stevens N, Dobbelaere J, Wainman A, Gergely F, Raff J. Ana3 is a conserved protein required for the structural integrity of centrioles and basal bodies. J Cell Biol. 2009;187:355-63 pubmed publisher
    ..Thus, Ana3 defines a conserved family of centriolar proteins and plays an important part in ensuring the structural integrity of centrioles and basal bodies. ..
  21. Rebollo E, Roldán M, Gonzalez C. Spindle alignment is achieved without rotation after the first cell cycle in Drosophila embryonic neuroblasts. Development. 2009;136:3393-7 pubmed publisher
    ..Therefore, predetermined spindle orientation is not restricted to larval NBs. On the contrary, it actually applies to all but the first cell cycle of embryonic NBs. ..
  22. Mottier Pavie V, Megraw T. Drosophila bld10 is a centriolar protein that regulates centriole, basal body, and motile cilium assembly. Mol Biol Cell. 2009;20:2605-14 pubmed publisher
    ..These results show that Drosophila Bld10 is required for centriole and axoneme assembly to confer cilium motility. ..
  23. Bonaccorsi S, Mottier V, Giansanti M, Bolkan B, Williams B, Goldberg M, et al. The Drosophila Lkb1 kinase is required for spindle formation and asymmetric neuroblast division. Development. 2007;134:2183-93 pubmed
    ..The finding that Dlkb1 mediates both the geometry of stem cell division and chromosome segregation provides novel insight into the mechanisms underlying tumor formation in Peutz-Jeghers patients. ..
  24. Conduit P, Wainman A, Novak Z, Weil T, Raff J. Re-examining the role of Drosophila Sas-4 in centrosome assembly using two-colour-3D-SIM FRAP. elife. 2015;4: pubmed publisher
    ..that supports mitotic PCM assembly; the recruitment of Cnn and Spd-2 is dependent on another S-CAP protein, Asl. We show here, however, that Cnn, Spd-2 and Asl are not recruited to the mother centriole as part of a complex with ..
  25. Klebba J, Galletta B, Nye J, Plevock K, Buster D, Hollingsworth N, et al. Two Polo-like kinase 4 binding domains in Asterless perform distinct roles in regulating kinase stability. J Cell Biol. 2015;208:401-14 pubmed publisher
    Plk4 (Polo-like kinase 4) and its binding partner Asterless (Asl) are essential, conserved centriole assembly factors that induce centriole amplification when overexpressed...
  26. Kaltschmidt J, Brand A. Asymmetric cell division: microtubule dynamics and spindle asymmetry. J Cell Sci. 2002;115:2257-64 pubmed
    ..In this commentary we discuss recent findings on how the mitotic spindle is positioned and on cleavage site induction and place them in the context of cell size asymmetry in different model organisms. ..
  27. Couturier L, Mazouni K, Schweisguth F. Numb localizes at endosomes and controls the endosomal sorting of notch after asymmetric division in Drosophila. Curr Biol. 2013;23:588-93 pubmed publisher
  28. Venkei Z, Yamashita Y. The centrosome orientation checkpoint is germline stem cell specific and operates prior to the spindle assembly checkpoint in Drosophila testis. Development. 2015;142:62-9 pubmed publisher
    ..This study may provide a framework for identifying and understanding similar mechanisms that might be in place in other asymmetrically dividing cell types. ..
  29. Wang H, Oliferenko S, Balasubramanian M. Cytokinesis: relative alignment of the cell division apparatus and the mitotic spindle. Curr Opin Cell Biol. 2003;15:82-7 pubmed
    ..The interaction(s) of astral microtubules with the cell cortex appears to play an important role in establishing perpendicularity between chromosome segregation and cell division machinery. ..
  30. Gallaud E, Caous R, Pascal A, Bazile F, Gagné J, Huet S, et al. Ensconsin/Map7 promotes microtubule growth and centrosome separation in Drosophila neural stem cells. J Cell Biol. 2014;204:1111-21 pubmed publisher
    ..In addition, Ensconsin promotes microtubule polymerization during mitosis to control spindle length independent of Kinesin-1. ..
  31. Carvajal Gonzalez J, Roman A, Mlodzik M. Positioning of centrioles is a conserved readout of Frizzled planar cell polarity signalling. Nat Commun. 2016;7:11135 pubmed publisher
    ..Together with vertebrate data, these results suggest a unifying model of centriole/cilia positioning as a common downstream effect of PCP signalling from flies to mammals. ..
  32. Chia W, Yang X. Asymmetric division of Drosophila neural progenitors. Curr Opin Genet Dev. 2002;12:459-64 pubmed
    ..Recent findings also suggest possible mechanisms by which the processes of cell-cycle progression, neuronal lineage development and asymmetric divisions may be integrated. ..
  33. Loppin B, Dubruille R, Horard B. The intimate genetics of Drosophila fertilization. Open Biol. 2015;5: pubmed publisher
  34. Pollarolo G, Schulz J, Munck S, Dotti C. Cytokinesis remnants define first neuronal asymmetry in vivo. Nat Neurosci. 2011;14:1525-33 pubmed publisher
    ..This is, to our knowledge, the first molecular study of initial neuronal polarization in vivo. The mechanisms of polarization seem to be defined at the precursor stage. ..
  35. GONCZY P. Mechanisms of spindle positioning: focus on flies and worms. Trends Cell Biol. 2002;12:332-9 pubmed
    ..This article discusses recent findings that indicate how this mechanism might be used for spindle positioning during Drosophila and Caenorhabditis elegans development. ..
  36. Smyth J, Schoborg T, Bergman Z, Riggs B, Rusan N. Proper symmetric and asymmetric endoplasmic reticulum partitioning requires astral microtubules. Open Biol. 2015;5: pubmed publisher
    ..Importantly, we show that the ER also associated with astral MTs in cultured human cells, suggesting that this centrosome/astral MT-based partitioning mechanism is conserved across animal species. ..
  37. Jauffred B, Llense F, Sommer B, Wang Z, Martin C, Bellaiche Y. Regulation of centrosome movements by numb and the collapsin response mediator protein during Drosophila sensory progenitor asymmetric division. Development. 2013;140:2657-68 pubmed publisher
  38. Zheng X, Gooi L, Wason A, Gabriel E, Mehrjardi N, Yang Q, et al. Conserved TCP domain of Sas-4/CPAP is essential for pericentriolar material tethering during centrosome biogenesis. Proc Natl Acad Sci U S A. 2014;111:E354-63 pubmed publisher
    ..Hence, we provide possible insights into how centrosomal protein defects result in human MCPH and how Sas-4 proteins act as a vehicle to tether PCM complexes to centrioles independent of its well-known role in centriole duplication. ..
  39. Kitazawa D, Yamaguchi M, Mori H, Inoue Y. COPI-mediated membrane trafficking is required for cytokinesis in Drosophila male meiotic divisions. J Cell Sci. 2012;125:3649-60 pubmed
    ..Thus, we propose that COPI plays an important role in Drosophila male meiosis, not only through vesicle transport to the cleavage furrow region, but also through the formation of ER-based structures. ..
  40. Sampaio P, Rebollo E, Varmark H, Sunkel C, Gonzalez C. Organized microtubule arrays in gamma-tubulin-depleted Drosophila spermatocytes. Curr Biol. 2001;11:1788-93 pubmed
    ..Finally, our results show that at least part of the central spindle and constriction-ring assembly machinery can operate on microtubule bundles that are not organized as bipolar spindles. ..
  41. Nashchekin D, Fernandes A, St Johnston D. Patronin/Shot Cortical Foci Assemble the Noncentrosomal Microtubule Array that Specifies the Drosophila Anterior-Posterior Axis. Dev Cell. 2016;38:61-72 pubmed publisher
    ..Thus, Shot/Patronin ncMTOCs may provide a general mechanism for organizing noncentrosomal microtubules in differentiated cells. ..
  42. Hayashi R, Wainwright S, Liddell S, Pinchin S, Horswell S, Ish Horowicz D. A genetic screen based on in vivo RNA imaging reveals centrosome-independent mechanisms for localizing gurken transcripts in Drosophila. G3 (Bethesda). 2014;4:749-60 pubmed publisher
    ..We also show that initiation of the dorsoanterior localization of grk mRNA precedes centrosome localization, suggesting that microtubule self-organization contributes to breaking axial symmetry to generate a unique dorsoventral axis. ..
  43. Miyauchi C, Kitazawa D, Ando I, Hayashi D, Inoue Y. Orbit/CLASP is required for germline cyst formation through its developmental control of fusomes and ring canals in Drosophila males. PLoS ONE. 2013;8:e58220 pubmed publisher
    ..Moreover, Orbit is involved in the proper orientation of spindles towards fusomes during synchronous mitosis of spermatogonial cysts. ..
  44. Kracklauer M, Wiora H, Deery W, Chen X, Bolival B, Romanowicz D, et al. The Drosophila SUN protein Spag4 cooperates with the coiled-coil protein Yuri Gagarin to maintain association of the basal body and spermatid nucleus. J Cell Sci. 2010;123:2763-72 pubmed publisher
    ..The later defects in spermatogenesis seen for yuri and spag4 mutants are similar, suggesting they could be secondary to initial disruption of events at the nuclear surface. ..
  45. Galletta B, Fagerstrom C, Schoborg T, McLamarrah T, Ryniawec J, Buster D, et al. A centrosome interactome provides insight into organelle assembly and reveals a non-duplication role for Plk4. Nat Commun. 2016;7:12476 pubmed publisher
    ..We show that Plk4 phosphorylates Cep135 to properly position the essential centriole component Asterless. This interaction landscape affords a critical framework for research of normal and aberrant centrosomes.
  46. Fu J, Lipinszki Z, Rangone H, Min M, Mykura C, Chao Chu J, et al. Conserved molecular interactions in centriole-to-centrosome conversion. Nat Cell Biol. 2016;18:87-99 pubmed publisher
    ..Here we show that centriole-to-centrosome conversion requires sequential loading of Cep135, Ana1 (Cep295) and Asterless (Cep152) onto daughter centrioles during mitotic progression in both Drosophila melanogaster and human...
  47. Reschen R, Colombié N, Wheatley L, Dobbelaere J, St Johnston D, Ohkura H, et al. Dgp71WD is required for the assembly of the acentrosomal Meiosis I spindle, and is not a general targeting factor for the γ-TuRC. Biol Open. 2012;1:422-9 pubmed publisher
    ..Thus, Dgp71WD is not simply a general cofactor required for γ-TuRC and/or Augmin targeting, and it appears to have a crucial role independent of these complexes in the acentrosomal Meiosis I spindle. ..
  48. Franz A, Roque H, Saurya S, Dobbelaere J, Raff J. CP110 exhibits novel regulatory activities during centriole assembly in Drosophila. J Cell Biol. 2013;203:785-99 pubmed publisher
    ..Finally, and unexpectedly, CP110 suppresses centriole overduplication induced by the overexpression of centriole duplication proteins. These studies identify novel and surprising functions for CP110 in vivo in flies. ..
  49. Mendes Maia T, Gogendeau D, Pennetier C, Janke C, Basto R. Bug22 influences cilium morphology and the post-translational modification of ciliary microtubules. Biol Open. 2014;3:138-51 pubmed publisher
    ..Our work identifies Bug22 as a protein that plays a conserved role in the regulation of PTMs of the ciliary axoneme. ..
  50. Wakefield J, Bonaccorsi S, Gatti M. The drosophila protein asp is involved in microtubule organization during spindle formation and cytokinesis. J Cell Biol. 2001;153:637-48 pubmed
    ..By examining cells that carry mutations both in asp and in asterless, a gene required for centrosome function, we have determined the role of Asp in the absence of centrosomes...
  51. Gottardo M, Pollarolo G, Llamazares S, Reina J, Riparbelli M, Callaini G, et al. Loss of Centrobin Enables Daughter Centrioles to Form Sensory Cilia in Drosophila. Curr Biol. 2015;25:2319-24 pubmed publisher
    ..Conversely, basal body capability is inhibited in mother centrioles modified to carry CNB. These results reveal the crucial role of CNB in regulating basal body function in Drosophila ciliated sensory organs. ..
  52. Conduit P, Richens J, Wainman A, Holder J, Vicente C, Pratt M, et al. A molecular mechanism of mitotic centrosome assembly in Drosophila. elife. 2014;3:e03399 pubmed publisher
    ..In this study, we show that the centriole protein Asl initiates the recruitment of DSpd-2 and Cnn to mother centrioles; both proteins then assemble into co-dependent ..
  53. Novak Z, Wainman A, Gartenmann L, Raff J. Cdk1 Phosphorylates Drosophila Sas-4 to Recruit Polo to Daughter Centrioles and Convert Them to Centrosomes. Dev Cell. 2016;37:545-57 pubmed publisher
    ..site that helps recruit Polo to daughter centrioles and is required for the subsequent recruitment of Asterless (Asl), a protein essential for centriole duplication and mitotic centrosome assembly...
  54. Christophorou N, Rubin T, Bonnet I, Piolot T, Arnaud M, Huynh J. Microtubule-driven nuclear rotations promote meiotic chromosome dynamics. Nat Cell Biol. 2015;17:1388-400 pubmed publisher
    ..Our findings reveal a mechanism for chromosome pairing in Drosophila, and indicate that microtubules, centrosomes and associated proteins play a crucial role in the dynamic organization of chromosomes inside the nucleus. ..
  55. Shimanovskaya E, Viscardi V, Lesigang J, Lettman M, Qiao R, Svergun D, et al. Structure of the C. elegans ZYG-1 cryptic polo box suggests a conserved mechanism for centriolar docking of Plk4 kinases. Structure. 2014;22:1090-1104 pubmed publisher
    ..between their cryptic polo box (CPB) and acidic regions in the centriolar receptors SPD-2/Cep192 and/or Asterless/Cep152. Here, we report a crystal structure for the CPB of C...
  56. Gopalakrishnan J, Chim Y, Ha A, Basiri M, Lerit D, Rusan N, et al. Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment. Nat Cell Biol. 2012;14:865-73 pubmed publisher
    ..On the basis of its guanine-bound state, tubulin can act as a molecular switch in PCM recruitment. ..
  57. Saurya S, Roque H, Novak Z, Wainman A, Aydogan M, Volanakis A, et al. Drosophila Ana1 is required for centrosome assembly and centriole elongation. J Cell Sci. 2016;129:2514-25 pubmed publisher
    ..that Cep135 (also known as Bld10) initially recruits Ana1 to newly formed centrioles, and that Ana1 then recruits Asl (known as Cep152 in mammals) to promote the conversion of these centrioles into centrosomes...
  58. Sousa Guimarães S, Sunkel C, Malmanche N. polo Is Identified as a Suppressor of bubR1 Nondisjunction in a Deficiency Screen of the Third Chromosome in Drosophila melanogaster. G3 (Bethesda). 2011;1:161-9 pubmed publisher
    ..Overall, our genetic screening strategy proved to be highly sensitive for the identification of modifiers of BubR1 kinase activity in both meiosis and mitosis. ..
  59. Klebba J, Buster D, McLamarrah T, Rusan N, Rogers G. Autoinhibition and relief mechanism for Polo-like kinase 4. Proc Natl Acad Sci U S A. 2015;112:E657-66 pubmed publisher
    ..Therefore, autoinhibition delays the multiple consequences of activation until Plk4 dimerizes. These findings reveal a complex mechanism of Plk4 regulation and activation which govern the process of centriole duplication. ..
  60. Baumbach J, Novak Z, Raff J, Wainman A. Dissecting the function and assembly of acentriolar microtubule organizing centers in Drosophila cells in vivo. PLoS Genet. 2015;11:e1005261 pubmed publisher
    ..show that aMTOCs can form in acentriolar Drosophila somatic cells in vivo via an assembly pathway that depends on Asl, Cnn and, to a lesser extent, Spd-2--the same proteins that appear to drive mitotic centrosome assembly in flies...
  61. Klebba J, Buster D, Nguyen A, Swatkoski S, Gucek M, Rusan N, et al. Polo-like kinase 4 autodestructs by generating its Slimb-binding phosphodegron. Curr Biol. 2013;23:2255-2261 pubmed publisher
    ..Taken together, our findings suggest that Plk4 directly generates its own phosphodegron and can do so without the assistance of an additional kinase(s). ..
  62. Galletta B, Jacobs K, Fagerstrom C, Rusan N. Asterless is required for centriole length control and sperm development. J Cell Biol. 2016;213:435-50 pubmed publisher
    ..Loss of the centriole protein Asterless (Asl), the Drosophila melanogaster orthologue of Cep152, prevents centriole duplication, which has limited the ..
  63. Dzhindzhev N, Tzolovsky G, Lipinszki Z, Schneider S, Lattao R, Fu J, et al. Plk4 phosphorylates Ana2 to trigger Sas6 recruitment and procentriole formation. Curr Biol. 2014;24:2526-32 pubmed publisher
  64. Lopes C, Jana S, Cunha Ferreira I, Zitouni S, Bento I, Duarte P, et al. PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space. Dev Cell. 2015;35:222-35 pubmed publisher
    ..Similar mechanisms enforcing the local concentration and/or activity of other centriole components are likely to contribute to the spatial control of centriole biogenesis under physiological conditions. ..
  65. Chen J, Kao L, Jana S, Sivan Loukianova E, Mendonça S, Cabrera O, et al. Rootletin organizes the ciliary rootlet to achieve neuron sensory function in Drosophila. J Cell Biol. 2015;211:435-53 pubmed publisher
    ..Ectopically expressed Root resides at the base of mother centrioles in spermatocytes and localizes asymmetrically to mother centrosomes in neuroblasts, both requiring Bld10, a basal body protein with varied functions. ..