AGO3

Summary

Gene Symbol: AGO3
Description: Argonaute 3
Alias: Ago-3, Ago3, BEST:LD15785, CG40300, Dmel\CG40300, ago3, dAgo3, argonaute 3, AGO3-PD, AGO3-PE, AGO3-PF, AGO3-PG, Argonaute-3, Argonaute3, BEST:LD15785, CG40300-PD, CG40300-PE, CG40300-PF, CG40300-PG
Species: fruit fly

Top Publications

  1. Zamore P. RNA silencing: genomic defence with a slice of pi. Nature. 2007;446:864-5 pubmed
  2. Zamparini A, Davis M, Malone C, Vieira E, Zavadil J, Sachidanandam R, et al. Vreteno, a gonad-specific protein, is essential for germline development and primary piRNA biogenesis in Drosophila. Development. 2011;138:4039-50 pubmed publisher
    ..In the germline, piRNAs can engage in an Aub- and Argonaute 3 (AGO3)-dependent amplification in the absence of Vret, suggesting that Vret function can distinguish between ..
  3. Lin H. piRNAs in the germ line. Science. 2007;316:397 pubmed
    ..Recently, a class of small RNAs that interact with Piwi proteins has been discovered in the mammalian germ line and Drosophila. These Piwi-interacting RNAs (piRNAs) represent a distinct small RNA pathway. ..
  4. Preall J, Czech B, Guzzardo P, Muerdter F, Hannon G. shutdown is a component of the Drosophila piRNA biogenesis machinery. RNA. 2012;18:1446-57 pubmed publisher
    ..Shu contains domains implicated in peptidyl-prolyl cis-trans isomerase activity and in the binding of HSP90-family chaperones, though the relevance of these domains to piRNA biogenesis is unknown. ..
  5. Khurana J, Wang J, Xu J, Koppetsch B, Thomson T, Nowosielska A, et al. Adaptation to P element transposon invasion in Drosophila melanogaster. Cell. 2011;147:1551-63 pubmed publisher
    ..P element invasion thus triggers heritable changes in genome structure that appear to enhance transposon silencing. ..
  6. Robine N, Lau N, Balla S, Jin Z, Okamura K, Kuramochi Miyagawa S, et al. A broadly conserved pathway generates 3'UTR-directed primary piRNAs. Curr Biol. 2009;19:2066-76 pubmed publisher
    ..These findings strongly increase the breadth of Argonaute-mediated small RNA systems in metazoans. ..
  7. Li C, Vagin V, Lee S, Xu J, Ma S, Xi H, et al. Collapse of germline piRNAs in the absence of Argonaute3 reveals somatic piRNAs in flies. Cell. 2009;137:509-21 pubmed publisher
    ..piRNA, bound to Aubergine or Piwi protein, triggers production of a sense piRNA bound to the PIWI protein Argonaute3 (Ago3). In turn, the new piRNA is envisioned to produce a second antisense piRNA...
  8. Obbard D, Gordon K, Buck A, Jiggins F. The evolution of RNAi as a defence against viruses and transposable elements. Philos Trans R Soc Lond B Biol Sci. 2009;364:99-115 pubmed publisher
    ..Over longer time scales, key RNAi genes are repeatedly duplicated or lost across the metazoan phylogeny, with important implications for RNAi as an immune defence. ..
  9. Saito K, Ishizu H, Komai M, Kotani H, Kawamura Y, Nishida K, et al. Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. Genes Dev. 2010;24:2493-8 pubmed publisher
    ..Thus, piRNA loading is required for Piwi nuclear entry. We propose that a functional Piwi-piRNA complex is formed and inspected in Yb bodies before its nuclear entry to exert transposon silencing. ..

More Information

Publications74

  1. Olivieri D, Sykora M, Sachidanandam R, Mechtler K, Brennecke J. An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila. EMBO J. 2010;29:3301-17 pubmed publisher
    ..Within germline cells, piRNAs are processed from piRNA clusters and transposons to be loaded into Piwi/Aubergine/AGO3 and a subset of piRNAs undergoes target-dependent amplification...
  2. Klenov M, Lavrov S, Stolyarenko A, Ryazansky S, Aravin A, Tuschl T, et al. Repeat-associated siRNAs cause chromatin silencing of retrotransposons in the Drosophila melanogaster germline. Nucleic Acids Res. 2007;35:5430-8 pubmed
    ..Our results provide evidence that germinal Piwi-associated short RNAs induce chromatin modifications of their targets. ..
  3. Arkov A, Ramos A. Building RNA-protein granules: insight from the germline. Trends Cell Biol. 2010;20:482-90 pubmed publisher
    ..These data suggest a widespread use of several protein motifs in germline development and further our understanding of other ribonucleoprotein structures, for example, processing bodies and neuronal granules. ..
  4. Khurana J, Xu J, Weng Z, Theurkauf W. Distinct functions for the Drosophila piRNA pathway in genome maintenance and telomere protection. PLoS Genet. 2010;6:e1001246 pubmed publisher
    ..Here we show that mutations in the Drosophila piRNA pathway genes, armi, aub, ago3, and rhi, lead to extensive fragmentation of the zygotic genome during the cleavage stage of embryonic divisions...
  5. Moshkovich N, Lei E. HP1 recruitment in the absence of argonaute proteins in Drosophila. PLoS Genet. 2010;6:e1000880 pubmed publisher
    ..Taken together, these results indicate that heterochromatin forms independently of endo-siRNA and piRNA pathways. ..
  6. Saito K, Inagaki S, Mituyama T, Kawamura Y, Ono Y, Sakota E, et al. A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila. Nature. 2009;461:1296-9 pubmed publisher
    ..RNAs (piRNAs) silence retrotransposons in Drosophila germ lines by associating with the PIWI proteins Argonaute 3 (AGO3), Aubergine (Aub) and Piwi...
  7. Rouget C, Papin C, Boureux A, Meunier A, Franco B, Robine N, et al. Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo. Nature. 2010;467:1128-32 pubmed publisher
    ..Because the piRNAs involved in this regulation are produced from transposable elements, this identifies a direct developmental function for transposable elements in the regulation of gene expression. ..
  8. Anand A, Kai T. The tudor domain protein kumo is required to assemble the nuage and to generate germline piRNAs in Drosophila. EMBO J. 2012;31:870-82 pubmed publisher
    ..Our data suggest that kumo facilitates germline piRNA production by promoting piRNA cluster transcription in the nucleus and piRNA processing at the nuage. ..
  9. Perrat P, Dasgupta S, Wang J, Theurkauf W, Weng Z, Rosbash M, et al. Transposition-driven genomic heterogeneity in the Drosophila brain. Science. 2013;340:91-5 pubmed publisher
    ..The Piwi-interacting RNA (piRNA) proteins Aubergine and Argonaute 3, known to suppress transposons in the fly germline, are expressed in the brain and appear less abundant in α..
  10. Vagin V, Sigova A, Li C, Seitz H, Gvozdev V, Zamore P. A distinct small RNA pathway silences selfish genetic elements in the germline. Science. 2006;313:320-4 pubmed
    ..Our data suggest that rasiRNAs protect the fly germline through a silencing mechanism distinct from both the miRNA and RNA interference pathways. ..
  11. Malone C, Brennecke J, Dus M, Stark A, McCombie W, Sachidanandam R, et al. Specialized piRNA pathways act in germline and somatic tissues of the Drosophila ovary. Cell. 2009;137:522-35 pubmed publisher
  12. Rozhkov N, Hammell M, Hannon G. Multiple roles for Piwi in silencing Drosophila transposons. Genes Dev. 2013;27:400-12 pubmed publisher
    Silencing of transposons in the Drosophila ovary relies on three Piwi family proteins--Piwi, Aubergine (Aub), and Ago3--acting in concert with their small RNA guides, the Piwi-interacting RNAs (piRNAs)...
  13. Kibanov M, Egorova K, Ryazansky S, Sokolova O, Kotov A, Olenkina O, et al. A novel organelle, the piNG-body, in the nuage of Drosophila male germ cells is associated with piRNA-mediated gene silencing. Mol Biol Cell. 2011;22:3410-9 pubmed publisher
    Proteins of the PIWI subfamily Aub and AGO3 associated with the germline-specific perinuclear granules (nuage) are involved in the silencing of retrotransposons and other selfish repetitive elements in the Drosophila genome...
  14. Lim A, Tao L, Kai T. piRNAs mediate posttranscriptional retroelement silencing and localization to pi-bodies in the Drosophila germline. J Cell Biol. 2009;186:333-42 pubmed publisher
    ..HeT-A mRNA is derepressed in mRNA degradation mutants twin, dcp1, and ski3, indicating that these enzymes also aid in removing full-length transcripts and/or decay intermediates. ..
  15. Brower Toland B, Findley S, Jiang L, Liu L, Yin H, Dus M, et al. Drosophila PIWI associates with chromatin and interacts directly with HP1a. Genes Dev. 2007;21:2300-11 pubmed
    ..These findings implicate a direct interaction between the PIWI-mediated small RNA mechanism and heterochromatin-forming pathways in determining the epigenetic state of the fly genome. ..
  16. Nagao A, Mituyama T, Huang H, Chen D, Siomi M, Siomi H. Biogenesis pathways of piRNAs loaded onto AGO3 in the Drosophila testis. RNA. 2010;16:2503-15 pubmed publisher
    ..b>AGO3, one of three PIWI proteins in flies, is involved in the ping-pong cycle in ovaries...
  17. Klattenhoff C, Xi H, Li C, Lee S, Xu J, Khurana J, et al. The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clusters. Cell. 2009;138:1137-49 pubmed publisher
    ..Rhino thus appears to promote transcription of dual-strand clusters, leading to production of piRNAs that drive the ping-pong amplification cycle. ..
  18. Lau N, Robine N, Martin R, Chung W, Niki Y, Berezikov E, et al. Abundant primary piRNAs, endo-siRNAs, and microRNAs in a Drosophila ovary cell line. Genome Res. 2009;19:1776-85 pubmed publisher
    ..These data indicate that the restriction of certain TEs in somatic gonadal cells is largely mediated by a primary piRNA pathway. ..
  19. Qi H, Watanabe T, Ku H, Liu N, Zhong M, Lin H. The Yb body, a major site for Piwi-associated RNA biogenesis and a gateway for Piwi expression and transport to the nucleus in somatic cells. J Biol Chem. 2011;286:3789-97 pubmed publisher
    ..Finally, we discovered putative endo-siRNAs in the flamenco locus and the Yb dependence of their expression. These observations further implicate a role for Yb in transposon silencing via both the piRNA and endo-siRNA pathways. ..
  20. Liu L, Qi H, Wang J, Lin H. PAPI, a novel TUDOR-domain protein, complexes with AGO3, ME31B and TRAL in the nuage to silence transposition. Development. 2011;138:1863-73 pubmed publisher
    ..Recently, the nuage in Drosophila was shown to contain two of the three PIWI proteins - Aubergine and Argonaute 3 (AGO3) - that are essential for germline development...
  21. Brennecke J, Aravin A, Stark A, Dus M, Kellis M, Sachidanandam R, et al. Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila. Cell. 2007;128:1089-103 pubmed
    ..Piwi protein and find that Piwi and Aubergine bind RNAs that are predominantly antisense to transposons, whereas Ago3 complexes contain predominantly sense piRNAs...
  22. Williams R, Rubin G. ARGONAUTE1 is required for efficient RNA interference in Drosophila embryos. Proc Natl Acad Sci U S A. 2002;99:6889-94 pubmed
    ..Furthermore, AGO1 is not required for siRNA production in vitro nor can the introduction of siRNA bypass AGO1 mutants in vivo. These data suggest that AGO1 functions downstream of siRNA production...
  23. Olivieri D, Senti K, Subramanian S, Sachidanandam R, Brennecke J. The cochaperone shutdown defines a group of biogenesis factors essential for all piRNA populations in Drosophila. Mol Cell. 2012;47:954-69 pubmed publisher
    ..Together with data on how PIWI proteins are wired into primary and secondary processing, we propose a unified model for piRNA biogenesis. ..
  24. Kawamura Y, Saito K, Kin T, Ono Y, Asai K, Sunohara T, et al. Drosophila endogenous small RNAs bind to Argonaute 2 in somatic cells. Nature. 2008;453:793-7 pubmed publisher
    ..Mutations in Dicer-2 caused an increase in retrotransposon transcripts. Together, our findings indicate that different types of small RNAs and Argonautes are used to repress retrotransposons in germline and somatic cells in Drosophila. ..
  25. Saito K, Sakaguchi Y, Suzuki T, Suzuki T, Siomi H, Siomi M. Pimet, the Drosophila homolog of HEN1, mediates 2'-O-methylation of Piwi- interacting RNAs at their 3' ends. Genes Dev. 2007;21:1603-8 pubmed
    ..Recombinant Pimet shows single-stranded small RNA methylation activity in vitro and interacts with the PIWI proteins within Pimet mutant ovary. These results show that Pimet mediates piRNA 2'-O-methylation in Drosophila. ..
  26. Grewal S, Elgin S. Transcription and RNA interference in the formation of heterochromatin. Nature. 2007;447:399-406 pubmed
    ..Silencing of chromatin might involve trans-acting sources of the crucial small RNAs that carry out RNA interference, but in some cases, transcription of the region to be silenced seems to be required--an apparent contradiction. ..
  27. Kavi H, Fernandez H, Xie W, Birchler J. RNA silencing in Drosophila. FEBS Lett. 2005;579:5940-9 pubmed
    ..RNA silencing processes in Drosophila are described. ..
  28. Zhang Z, Xu J, Koppetsch B, Wang J, Tipping C, Ma S, et al. Heterotypic piRNA Ping-Pong requires qin, a protein with both E3 ligase and Tudor domains. Mol Cell. 2011;44:572-84 pubmed publisher
    ..Reciprocal cycles of piRNA-directed RNA cleavage--catalyzed by the PIWI proteins Aubergine (Aub) and Argonaute3 (Ago3) in Drosophila melanogaster--expand the population of antisense piRNAs in response to transposon ..
  29. Nishida K, Okada T, Kawamura T, Mituyama T, Kawamura Y, Inagaki S, et al. Functional involvement of Tudor and dPRMT5 in the piRNA processing pathway in Drosophila germlines. EMBO J. 2009;28:3820-31 pubmed publisher
    In Drosophila, the PIWI proteins, Aubergine (Aub), AGO3, and Piwi are expressed in germlines and function in silencing transposons by associating with PIWI-interacting RNAs (piRNAs)...
  30. Gunawardane L, Saito K, Nishida K, Miyoshi K, Kawamura Y, Nagami T, et al. A slicer-mediated mechanism for repeat-associated siRNA 5' end formation in Drosophila. Science. 2007;315:1587-90 pubmed
    ..We sequenced small RNAs associated with the PIWI subfamily member AGO3. Although other members of PIWI, Aubergine (Aub) and Piwi, associated with rasiRNAs derived mainly from the ..
  31. Kirino Y, Kim N, De Planell Saguer M, Khandros E, Chiorean S, Klein P, et al. Arginine methylation of Piwi proteins catalysed by dPRMT5 is required for Ago3 and Aub stability. Nat Cell Biol. 2009;11:652-8 pubmed publisher
    ..which is also the product of a grandchildless gene, is required for arginine methylation of Drosophila Piwi, Ago3 and Aub proteins in vivo...
  32. Patil V, Kai T. Repression of retroelements in Drosophila germline via piRNA pathway by the Tudor domain protein Tejas. Curr Biol. 2010;20:724-30 pubmed publisher
    ..15] and spindle-E (spn-E) [1, 16, 17] while it regulates the localization of Spn-E, Aubergine (Aub) [3, 4, 14], Argonaute3 (Ago3) [5], Krimper (Krimp) [13], and Maelstrom (Mael) [18] to nuage...
  33. Rashpa R, Vazquez Pianzola P, Colombo M, Hernández G, Beuchle D, Berger F, et al. Cbp80 is needed for the expression of piRNA components and piRNAs. PLoS ONE. 2017;12:e0181743 pubmed publisher
    ..that Cbp80 knockdown not only led to the upregulation of transposons, but also to delocalization of Piwi, Aub and Ago3, key factors in the piRNA biosynthesis pathway...
  34. Han B, Wang W, Li C, Weng Z, Zamore P. Noncoding RNA. piRNA-guided transposon cleavage initiates Zucchini-dependent, phased piRNA production. Science. 2015;348:817-21 pubmed publisher
    ..We report that secondary piRNAs, bound to the PIWI protein Ago3, can initiate primary piRNA production from cleaved transposon RNAs...
  35. Barrón M, Fiston Lavier A, Petrov D, González J. Population genomics of transposable elements in Drosophila. Annu Rev Genet. 2014;48:561-81 pubmed publisher
    ..melanogaster. Although most of the TE insertions are deleterious or neutral, some TE insertions increase the fitness of the individual that carries them and play a role in genome adaptation. ..
  36. Huang H, Li Y, Szulwach K, Zhang G, Jin P, Chen D. AGO3 Slicer activity regulates mitochondria-nuage localization of Armitage and piRNA amplification. J Cell Biol. 2014;206:217-30 pubmed publisher
    ..catalyzed by the endonuclease (or "Slicer") activities of the PIWI proteins Aubergine (Aub) and Argonaute3 (AGO3) has been proposed to expand the secondary piRNA population...
  37. Richardson S, Morell S, Faulkner G. L1 retrotransposons and somatic mosaicism in the brain. Annu Rev Genet. 2014;48:1-27 pubmed publisher
    ..Finally, we discuss the unresolved impact of L1-mediated somatic mosaicism upon normal neurobiology, as well as its potential to drive neurological disease. ..
  38. Gell S, Reenan R. Mutations to the piRNA pathway component aubergine enhance meiotic drive of segregation distorter in Drosophila melanogaster. Genetics. 2013;193:771-84 pubmed publisher
    ..Rsp sequences were found as small RNAs in association with Argonaute family proteins Aubergine (Aub) and Argonaute3 (AGO3)...
  39. Miyoshi K, Okada T, Siomi H, Siomi M. Characterization of the miRNA-RISC loading complex and miRNA-RISC formed in the Drosophila miRNA pathway. RNA. 2009;15:1282-91 pubmed publisher
    ..The AGO1-PIWI mutant showed no Slicer activity but associates with mature miRNA. These results indicate that these domains are required differently for miRLC and miRISC formation in the miRNA pathway. ..
  40. Kibanov M, Gvozdev V, Olenina L. Germ granules in spermatogenesis of Drosophila: Evidences of contribution to the piRNA silencing. Commun Integr Biol. 2012;5:130-3 pubmed publisher
    ..Proteins of the PIWI clade, Aub and AGO3, concentrated in the germline-specific perinuclear granules called nuage, are involved in silencing of ..
  41. Donertas D, Sienski G, Brennecke J. Drosophila Gtsf1 is an essential component of the Piwi-mediated transcriptional silencing complex. Genes Dev. 2013;27:1693-705 pubmed publisher
    ..We propose that only a small fraction of nuclear Piwi is actively engaged in target silencing and that Gtsf1 is an essential component of the underlying Piwi-centered silencing complex. ..
  42. Fablet M, Akkouche A, Braman V, Vieira C. Variable expression levels detected in the Drosophila effectors of piRNA biogenesis. Gene. 2014;537:149-53 pubmed publisher
    ..We showed that these genes exhibited wide variation in transcript levels, and we discuss some evolutionary considerations regarding the observed variability in TE copy numbers...
  43. Nagao A, Sato K, Nishida K, Siomi H, Siomi M. Gender-Specific Hierarchy in Nuage Localization of PIWI-Interacting RNA Factors in Drosophila. Front Genet. 2011;2:55 pubmed publisher
    ..This suggests that the functional requirement of Krimp in piRNA biogenesis may be different in male and female gonads. ..
  44. Dueck A, Meister G. Assembly and function of small RNA - argonaute protein complexes. Biol Chem. 2014;395:611-29 pubmed publisher
    ..This review will focus on the mechanisms of Argonaute loading in different organisms. Furthermore, we highlight the versatile functions of small RNA-Argonaute protein complexes in organisms from all three kingdoms of life. ..
  45. Gao M, Arkov A. Next generation organelles: structure and role of germ granules in the germline. Mol Reprod Dev. 2013;80:610-23 pubmed publisher
  46. Zhang C, Robinson B, Xu W, Yang L, Yao B, Zhao H, et al. The ecdysone receptor coactivator Taiman links Yorkie to transcriptional control of germline stem cell factors in somatic tissue. Dev Cell. 2015;34:168-80 pubmed publisher
    ..These data reveal Hippo/Ec pathway crosstalk in the form a Yki-Tai complex that collaboratively induces germline genes as part of a transcriptional program that is normally repressed in developing somatic epithelia. ..
  47. Goriaux C, Théron E, Brasset E, Vaury C. History of the discovery of a master locus producing piRNAs: the flamenco/COM locus in Drosophila melanogaster. Front Genet. 2014;5:257 pubmed publisher
    ..Here we review the first discovery of this locus and retrace decades of studies that led to our current understanding of the relationship between genomes and their TE targets. ..
  48. Wang W, Han B, Tipping C, Ge D, Zhang Z, Weng Z, et al. Slicing and Binding by Ago3 or Aub Trigger Piwi-Bound piRNA Production by Distinct Mechanisms. Mol Cell. 2015;59:819-30 pubmed publisher
    In Drosophila ovarian germ cells, PIWI-interacting RNAs (piRNAs) direct Aubergine and Argonaute3 to cleave transposon transcripts and instruct Piwi to repress transposon transcription, thereby safeguarding the germline genome...
  49. Bozzetti M, Fanti L, Di Tommaso S, Piacentini L, Berloco M, Tritto P, et al. The "Special" crystal-Stellate System in Drosophila melanogaster Reveals Mechanisms Underlying piRNA Pathway-Mediated Canalization. Genet Res Int. 2012;2012:324293 pubmed publisher
    ..In particular, we illustrate the finding that HSP90 participates in the molecular pathways of piRNA production. This observation has relevance for the mechanisms underlying the evolutionary canalization process. ..
  50. Zhang Z, Koppetsch B, Wang J, Tipping C, Weng Z, Theurkauf W, et al. Antisense piRNA amplification, but not piRNA production or nuage assembly, requires the Tudor-domain protein Qin. EMBO J. 2014;33:536-9 pubmed publisher
  51. Zhang F, Wang J, Xu J, Zhang Z, Koppetsch B, Schultz N, et al. UAP56 couples piRNA clusters to the perinuclear transposon silencing machinery. Cell. 2012;151:871-884 pubmed publisher
    ..Vasa has an established role in piRNA production and localizes to nuage with the piRNA binding PIWI proteins Ago3 and Aub...
  52. Larracuente A. The organization and evolution of the Responder satellite in species of the Drosophila melanogaster group: dynamic evolution of a target of meiotic drive. BMC Evol Biol. 2014;14:233 pubmed publisher
    ..melanogaster suggests that SD evolved after its target satellite and that the evolution of the Rsp satellite family is highly dynamic over a short evolutionary time scale (<240,000 years). ..
  53. Wood J, Helfand S. Chromatin structure and transposable elements in organismal aging. Front Genet. 2013;4:274 pubmed publisher
    ..This review summarizes the recent evidence linking chromatin structure and function to aging, with a particular focus on the relationship of heterochromatin structure to organismal aging. ..
  54. Petit M, Mongelli V, Frangeul L, Blanc H, Jiggins F, Saleh M. piRNA pathway is not required for antiviral defense in Drosophila melanogaster. Proc Natl Acad Sci U S A. 2016;113:E4218-27 pubmed publisher
  55. Pane A, Jiang P, Zhao D, Singh M, Schupbach T. The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline. EMBO J. 2011;30:4601-15 pubmed publisher
    ..Our results unveil a link between Cuff activity, heterochromatin assembly and piRNA cluster expression, which is critical for stem-cell and germ-cell development in Drosophila. ..
  56. Patil V, Anand A, Chakrabarti A, Kai T. The Tudor domain protein Tapas, a homolog of the vertebrate Tdrd7, functions in the piRNA pathway to regulate retrotransposons in germline of Drosophila melanogaster. BMC Biol. 2014;12:61 pubmed publisher
    ..show that Tap physically interacts with piRNA pathway components, such as Piwi family proteins Aubergine and Argonaute3 and the RNA helicases Spindle-E and Vasa...
  57. Morgunova V, Akulenko N, Radion E, Olovnikov I, Abramov Y, Olenina L, et al. Telomeric repeat silencing in germ cells is essential for early development in Drosophila. Nucleic Acids Res. 2015;43:8762-73 pubmed publisher
    ..Our data demonstrate that Ccr4-Not, Woc, Trf2 and Ars2, components of different regulatory pathways, are required for telomere protection in the germline in order to guarantee normal development. ..
  58. Lewis S, Salmela H, Obbard D. Duplication and Diversification of Dipteran Argonaute Genes, and the Evolutionary Divergence of Piwi and Aubergine. Genome Biol Evol. 2016;8:507-18 pubmed publisher
    ..Ago2) and germline suppression of transposable elements is mediated by a trio of Piwi-subfamily Argonaute proteins (Ago3, Aub, and Piwi)...
  59. Stefanov Y, Salenko V, Glukhov I. Drosophila errantiviruses. Mob Genet Elements. 2012;2:36-45 pubmed
  60. Samji T. PIWI, piRNAs, and germline stem cells: what's the link?. Yale J Biol Med. 2009;82:121-4 pubmed
  61. Soares Z, Gonçalves A, de Oliveira K, Marques J. Viral RNA recognition by the Drosophila small interfering RNA pathway. Microbes Infect. 2014;16:1013-21 pubmed publisher
    ..This antiviral response in fruit flies can help understand general principles of nucleic acid recognition. ..
  62. Mugat B, Akkouche A, Serrano V, Armenise C, Li B, Brun C, et al. MicroRNA-Dependent Transcriptional Silencing of Transposable Elements in Drosophila Follicle Cells. PLoS Genet. 2015;11:e1005194 pubmed publisher
    ..This work is one of the examples of detectable phenotypes caused by loss of individual miRNAs in Drosophila and the first genetic evidence that miRNAs have a role in the maintenance of genome stability via piRNA-mediated TE repression. ..
  63. Kibanov M, Kotov A, Olenina L. Multicolor fluorescence imaging of whole-mount Drosophila testes for studying spermatogenesis. Anal Biochem. 2013;436:55-64 pubmed publisher
    ..It allows one to combine the simultaneous detection of fluorescently tagged and immunostained proteins as well as TUNEL analysis for apoptosis detection. ..
  64. Franssen S, Nolte V, Tobler R, Schlotterer C. Patterns of linkage disequilibrium and long range hitchhiking in evolving experimental Drosophila melanogaster populations. Mol Biol Evol. 2015;32:495-509 pubmed publisher
  65. Simkin A, Wong A, Poh Y, Theurkauf W, Jensen J. Recurrent and recent selective sweeps in the piRNA pathway. Evolution. 2013;67:1081-90 pubmed publisher
    ..We speculate that selection on these proteins reflects crucial roles in silencing unfamiliar elements during vertical and horizontal transmission of TEs into naïve populations and species, respectively. ..