AGO2

Summary

Gene Symbol: AGO2
Description: Argonaute 2
Alias: AG02, AGO 2, Ago-2, Ago2, CG13452, CG7439, Dm Ago2, DmAgo2, Dmel\CG7439, ago, ago-2, ago2, dAGO2, dAgo2, eIF2C2, argonaute 2, AGO2-PB, AGO2-PC, AGO2-PE, ARGONAUTE2, CG7439-PB, CG7439-PC, CG7439-PE, argonaute, argonaute-2
Species: fruit fly
Products:     AGO2

Top Publications

  1. Matranga C, Tomari Y, Shin C, Bartel D, Zamore P. Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes. Cell. 2005;123:607-20 pubmed
    In the Drosophila and mammalian RNA interference pathways, siRNAs direct the protein Argonaute2 (Ago2) to cleave corresponding mRNA targets, silencing their expression. Ago2 is the catalytic component of the RNAi enzyme complex, RISC...
  2. Kataoka Y, Takeichi M, Uemura T. Developmental roles and molecular characterization of a Drosophila homologue of Arabidopsis Argonaute1, the founder of a novel gene superfamily. Genes Cells. 2001;6:313-25 pubmed
    ..Our results suggest that the dAGO1 protein exerts its developmental functions by binding to RNA either directly or indirectly. ..
  3. Miyoshi K, Miyoshi T, Hartig J, Siomi H, Siomi M. Molecular mechanisms that funnel RNA precursors into endogenous small-interfering RNA and microRNA biogenesis pathways in Drosophila. RNA. 2010;16:506-15 pubmed publisher
    ..The Dicer2-Loqs-PD complex processes esiRNA precursor hairpins with long stems, which results in the production of AGO2-associated small RNAs...
  4. Fukunaga R, Han B, Hung J, Xu J, Weng Z, Zamore P. Dicer partner proteins tune the length of mature miRNAs in flies and mammals. Cell. 2012;151:533-46 pubmed publisher
    ..Thus, Dicer-binding partner proteins change the choice of cleavage site by Dicer, producing miRNAs with target specificities different from those made by Dicer alone or Dicer bound to alternative protein partners. ..
  5. Horwich M, Li C, Matranga C, Vagin V, Farley G, Wang P, et al. The Drosophila RNA methyltransferase, DmHen1, modifies germline piRNAs and single-stranded siRNAs in RISC. Curr Biol. 2007;17:1265-72 pubmed
    ..2'-O-methylation of siRNAs may be the final step in assembly of the RNAi-enzyme complex, RISC, occurring after an Argonaute-bound siRNA duplex is converted to single-stranded RNA.
  6. Iwasaki S, Kobayashi M, Yoda M, Sakaguchi Y, Katsuma S, Suzuki T, et al. Hsc70/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes. Mol Cell. 2010;39:292-9 pubmed publisher
    ..Both siRNAs and miRNAs are born double stranded. Surprisingly, loading these small RNA duplexes into Argonaute proteins, the core components of RISC, requires ATP, whereas separating the two small RNA strands within ..
  7. van Mierlo J, Overheul G, Obadia B, van Cleef K, Webster C, Saleh M, et al. Novel Drosophila viruses encode host-specific suppressors of RNAi. PLoS Pathog. 2014;10:e1004256 pubmed publisher
    ..We have recently shown that viral protein 1 (VP1) of Drosophila melanogaster Nora virus (DmelNV) suppresses Argonaute-2 (AGO2)-mediated target RNA cleavage (slicer activity) to antagonize antiviral RNAi...
  8. Saleh M, Tassetto M, Van Rij R, Goic B, Gausson V, Berry B, et al. Antiviral immunity in Drosophila requires systemic RNA interference spread. Nature. 2009;458:346-50 pubmed publisher
    ..Thus, similar to protein-based immunity in vertebrates, the antiviral RNAi response in flies also relies on the systemic spread of a virus-specific immunity signal. ..
  9. Tomari Y, Du T, Zamore P. Sorting of Drosophila small silencing RNAs. Cell. 2007;130:299-308 pubmed
    In Drosophila, small interfering RNAs (siRNAs), which direct RNA interference through the Argonaute protein Ago2, are produced by a biogenesis pathway distinct from microRNAs (miRNAs), which regulate endogenous mRNA expression as guides ..

More Information

Publications91

  1. Nayak A, Berry B, Tassetto M, Kunitomi M, Acevedo A, Deng C, et al. Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila. Nat Struct Mol Biol. 2010;17:547-54 pubmed publisher
    ..In contrast, the CrPV suppressor (CrPV-1A) interacts with the endonuclease Argonaute 2 (Ago2) and inhibits its activity without affecting the microRNA (miRNA)-Ago1-mediated silencing...
  2. Ni J, Zhou R, Czech B, Liu L, Holderbaum L, Yang Zhou D, et al. A genome-scale shRNA resource for transgenic RNAi in Drosophila. Nat Methods. 2011;8:405-7 pubmed publisher
    ..We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource. ..
  3. Ghildiyal M, Xu J, Seitz H, Weng Z, Zamore P. Sorting of Drosophila small silencing RNAs partitions microRNA* strands into the RNA interference pathway. RNA. 2010;16:43-56 pubmed publisher
    ..RNAs are sorted between Argonaute1 (Ago1), the central protein component of the microRNA (miRNA) pathway, and Argonaute2 (Ago2), which mediates RNA interference...
  4. Ulvila J, Parikka M, Kleino A, Sormunen R, Ezekowitz R, Kocks C, et al. Double-stranded RNA is internalized by scavenger receptor-mediated endocytosis in Drosophila S2 cells. J Biol Chem. 2006;281:14370-5 pubmed
    ..We identified Argonaute 2, a core component of the RNAi machinery, and three gene products previously unknown to be involved in RNAi in ..
  5. Martin R, Smibert P, Yalcin A, Tyler D, Schäfer U, Tuschl T, et al. A Drosophila pasha mutant distinguishes the canonical microRNA and mirtron pathways. Mol Cell Biol. 2009;29:861-70 pubmed publisher
    ..These data suggest limitations to somatic clonal analysis of miRNA pathway components. ..
  6. Sabin L, Zhou R, Gruber J, Lukinova N, Bambina S, Berman A, et al. Ars2 regulates both miRNA- and siRNA- dependent silencing and suppresses RNA virus infection in Drosophila. Cell. 2009;138:340-51 pubmed publisher
    ..The identification of Ars2 as a player in these small RNA pathways provides new insight into the biogenesis of small RNAs that may be extended to other systems. ..
  7. Okamura K, Liu N, Lai E. Distinct mechanisms for microRNA strand selection by Drosophila Argonautes. Mol Cell. 2009;36:431-44 pubmed publisher
    ..strand preferences for miRNA duplexes, and that in particular, many miRNA* species accumulate in the RNAi effector AGO2. AGO2-loaded miRNA* species require canonical RNAi factors for their accumulation, are efficiently 3' modified, and ..
  8. Jing Q, Huang S, Guth S, Zarubin T, Motoyama A, Chen J, et al. Involvement of microRNA in AU-rich element-mediated mRNA instability. Cell. 2005;120:623-34 pubmed
    ..An RNAi-based screen performed in Drosophila S2 cells has revealed that Dicer1, Argonaute1 (Ago1) and Ago2, components involved in microRNA (miRNA) processing and function, are required for the rapid decay of mRNA ..
  9. Taliaferro J, Aspden J, Bradley T, Marwha D, Blanchette M, Rio D. Two new and distinct roles for Drosophila Argonaute-2 in the nucleus: alternative pre-mRNA splicing and transcriptional repression. Genes Dev. 2013;27:378-89 pubmed publisher
    ..Here we show that the small RNA pathway protein Argonaute-2 (Ago-2) regulates alternative pre-mRNA splicing patterns of specific transcripts in the Drosophila nucleus ..
  10. Barbee S, Estes P, Cziko A, Hillebrand J, Luedeman R, Coller J, et al. Staufen- and FMRP-containing neuronal RNPs are structurally and functionally related to somatic P bodies. Neuron. 2006;52:997-1009 pubmed
    ..bodies," including the RNA-degradative enzymes Dcp1p and Xrn1p/Pacman and crucial components of miRNA (argonaute), NMD (Upf1p), and general translational repression (Dhh1p/Me31B) pathways...
  11. Kim K, Lee Y, Carthew R. Conversion of pre-RISC to holo-RISC by Ago2 during assembly of RNAi complexes. RNA. 2007;13:22-9 pubmed
    In the Drosophila RNA interference (RNAi) pathway, small interfering RNAs (siRNAs) direct Argonaute2 (Ago2), an endonuclease, within the RNA-induced silencing complex (RISC) to cleave complementary mRNA targets...
  12. Leuschner P, Obernosterer G, Martinez J. MicroRNAs: Loquacious speaks out. Curr Biol. 2005;15:R603-5 pubmed
    ..New data show that Dicer-1 also requires a double-stranded RNA binding protein called Loquacious for efficient microRNA-mediated gene silencing. ..
  13. Fagegaltier D, Bouge A, Berry B, Poisot E, Sismeiro O, Coppee J, et al. The endogenous siRNA pathway is involved in heterochromatin formation in Drosophila. Proc Natl Acad Sci U S A. 2009;106:21258-63 pubmed publisher
    ..Similar effects were observed in dcr2, r2d2, and ago2 mutants...
  14. Van Rij R, Saleh M, Berry B, Foo C, Houk A, Antoniewski C, et al. The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster. Genes Dev. 2006;20:2985-95 pubmed
    ..Here we show that the central catalytic component of the RNA-induced silencing complex (RISC), the nuclease Argonaute 2 (Ago-2), is essential for antiviral defense in adult Drosophila melanogaster...
  15. Iwasaki S, Kawamata T, Tomari Y. Drosophila argonaute1 and argonaute2 employ distinct mechanisms for translational repression. Mol Cell. 2009;34:58-67 pubmed publisher
    ..have established an in vitro system that recapitulates translational repression mediated by the two Drosophila Argonaute (Ago) subfamily proteins, Ago1 and Ago2...
  16. González J, Lenkov K, Lipatov M, Macpherson J, Petrov D. High rate of recent transposable element-induced adaptation in Drosophila melanogaster. PLoS Biol. 2008;6:e251 pubmed publisher
    ..Such a high rate of TE-induced adaptation is inconsistent with the number of fixed TEs in the D. melanogaster genome, and we discuss possible explanations for this discrepancy. ..
  17. Wang X, Aliyari R, Li W, Li H, Kim K, Carthew R, et al. RNA interference directs innate immunity against viruses in adult Drosophila. Science. 2006;312:452-4 pubmed
    ..viral double-stranded RNA produced during infection acts as the pathogen trigger whereas Drosophila Dicer-2 and Argonaute-2 act as host sensor and effector, respectively...
  18. Vagin V, Sigova A, Li C, Seitz H, Gvozdev V, Zamore P. A distinct small RNA pathway silences selfish genetic elements in the germline. Science. 2006;313:320-4 pubmed
    ..Unlike siRNAs and miRNAs, rasiRNAs function through the Piwi, rather than the Ago, Argonaute protein subfamily...
  19. Pham J, Pellino J, Lee Y, Carthew R, Sontheimer E. A Dicer-2-dependent 80s complex cleaves targeted mRNAs during RNAi in Drosophila. Cell. 2004;117:83-94 pubmed
    ..Dcr-2 does not simply transfer siRNAs to a distinct effector complex, but rather assembles into RISC along with the siRNAs, indicating that its role extends beyond the initiation phase of RNAi. ..
  20. Ameres S, Horwich M, Hung J, Xu J, Ghildiyal M, Weng Z, et al. Target RNA-directed trimming and tailing of small silencing RNAs. Science. 2010;328:1534-9 pubmed publisher
    ..typically guide Argonaute1 to repress messenger RNA (mRNA), whereas small interfering RNAs (siRNAs) guide Argonaute2 to destroy viral and transposon RNA...
  21. Graze R, McIntyre L, Main B, Wayne M, Nuzhdin S. Regulatory divergence in Drosophila melanogaster and D. simulans, a genomewide analysis of allele-specific expression. Genetics. 2009;183:547-61, 1SI-21SI pubmed publisher
    ..In addition, two genes, with critical roles in sex determination and micro RNA processing, Sxl and loqs, were identified as misexpressed in hybrid female heads, potentially contributing to hybrid incompatibility. ..
  22. Nishida K, Miyoshi K, Ogino A, Miyoshi T, Siomi H, Siomi M. Roles of R2D2, a cytoplasmic D2 body component, in the endogenous siRNA pathway in Drosophila. Mol Cell. 2013;49:680-91 pubmed publisher
    Endogenous small interfering RNAs (endo-siRNAs) in Drosophila are processed by Dicer-2 (Dcr-2) and loaded onto Ago2 by the Dcr-2/R2D2 heterodimer...
  23. Rand T, Petersen S, Du F, Wang X. Argonaute2 cleaves the anti-guide strand of siRNA during RISC activation. Cell. 2005;123:621-9 pubmed
    The mRNA-cleavage step of RNA interference is mediated by an endonuclease, Argonaute2 (Ago2), within the RNA-induced silencing complex (RISC)...
  24. Findley S, Tamanaha M, Clegg N, Ruohola Baker H. Maelstrom, a Drosophila spindle-class gene, encodes a protein that colocalizes with Vasa and RDE1/AGO1 homolog, Aubergine, in nuage. Development. 2003;130:859-71 pubmed
    ..Furthermore, maelstrom mutant ovaries show mislocalization of two proteins involved in the microRNA and/or RNAi pathways, Dicer and Argonaute2, suggesting a potential connection between nuage and the microRNA-pathway.
  25. Liu Q, Rand T, Kalidas S, Du F, Kim H, Smith D, et al. R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway. Science. 2003;301:1921-5 pubmed
    ..These results indicate that R2D2 bridges the initiation and effector steps of the Drosophila RNAi pathway by facilitating siRNA passage from Dicer to RISC. ..
  26. Kolaczkowski B, Hupalo D, Kern A. Recurrent adaptation in RNA interference genes across the Drosophila phylogeny. Mol Biol Evol. 2011;28:1033-42 pubmed publisher
    ..Our observations suggest a predictive model of how selective pressures generated by evolutionary arms race scenarios may affect multiple genes across protein interaction networks and other biochemical pathways. ..
  27. Bronkhorst A, Van Rij R. The long and short of antiviral defense: small RNA-based immunity in insects. Curr Opin Virol. 2014;7:19-28 pubmed publisher
    ..Here, we review recent insights into the mechanism of antiviral RNAi, viral small RNA profiles, and viral counter-defense mechanisms in insects. ..
  28. Abe M, Naqvi A, Hendriks G, Feltzin V, Zhu Y, Grigoriev A, et al. Impact of age-associated increase in 2'-O-methylation of miRNAs on aging and neurodegeneration in Drosophila. Genes Dev. 2014;28:44-57 pubmed publisher
    ..Small RNA deep sequencing revealed a global increase of miRNAs loaded into Ago2, but not into Ago1, with age...
  29. Marques J, Wang J, Wang X, de Oliveira K, Gao C, Aguiar E, et al. Functional specialization of the small interfering RNA pathway in response to virus infection. PLoS Pathog. 2013;9:e1003579 pubmed publisher
    ..siRNAs are then loaded onto Argonaute-2 (Ago2) by the action of Dcr-2 with another dsRBP cofactor called R2D2...
  30. Haley B, Hendrix D, Trang V, Levine M. A simplified miRNA-based gene silencing method for Drosophila melanogaster. Dev Biol. 2008;321:482-90 pubmed publisher
    ..As proof of concept, this method was used for the efficient silencing of dpp gene activity in the adult wing, and the analysis of the general RNA Polymerase II (Pol II) elongation factor, Nelf-E. ..
  31. Ghildiyal M, Seitz H, Horwich M, Li C, Du T, Lee S, et al. Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells. Science. 2008;320:1077-81 pubmed publisher
    ..of somatic endo-siRNAs requires the siRNA-generating ribonuclease Dicer-2 and the RNAi effector protein Argonaute2 (Ago2)...
  32. Mueller S, Gausson V, Vodovar N, Deddouche S, Troxler L, Perot J, et al. RNAi-mediated immunity provides strong protection against the negative-strand RNA vesicular stomatitis virus in Drosophila. Proc Natl Acad Sci U S A. 2010;107:19390-5 pubmed publisher
    ..This result is of particular interest in view of the frequent transmission of medically relevant negative-strand RNA viruses to humans by insect vectors. ..
  33. Song J, Liu J, Tolia N, Schneiderman J, Smith S, Martienssen R, et al. The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes. Nat Struct Biol. 2003;10:1026-32 pubmed
    ..We have solved the crystal structure of the PAZ domain of Drosophila Argonaute2. The PAZ domain contains a variant of the OB fold, a module that often binds single-stranded nucleic acids...
  34. Moshkovich N, Lei E. HP1 recruitment in the absence of argonaute proteins in Drosophila. PLoS Genet. 2010;6:e1000880 pubmed publisher
    ..In order to determine if these Argonaute genes are required for heterochromatin formation, we utilized transcriptional reporters and chromatin ..
  35. Okamura K, Ishizuka A, Siomi H, Siomi M. Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways. Genes Dev. 2004;18:1655-66 pubmed
    ..In Drosophila, Argonaute2 (AGO2) was identified as part of the RISC complex...
  36. Robine N, Lau N, Balla S, Jin Z, Okamura K, Kuramochi Miyagawa S, et al. A broadly conserved pathway generates 3'UTR-directed primary piRNAs. Curr Biol. 2009;19:2066-76 pubmed publisher
    ..These findings strongly increase the breadth of Argonaute-mediated small RNA systems in metazoans.
  37. Ishizuka A, Siomi M, Siomi H. A Drosophila fragile X protein interacts with components of RNAi and ribosomal proteins. Genes Dev. 2002;16:2497-508 pubmed
    ..The dFMR1 complex also contains Argonaute2 (AGO2) and a Drosophila homolog of p68 RNA helicase (Dmp68)...
  38. Han Y, Luo Y, Wu Q, Jovel J, Wang X, Aliyari R, et al. RNA-based immunity terminates viral infection in adult Drosophila in the absence of viral suppression of RNA interference: characterization of viral small interfering RNA populations in wild-type and mutant flies. J Virol. 2011;85:13153-63 pubmed publisher
    ..FHV?B2 replicated to high levels and induced high mortality rates in dicer-2 and argonaute-2 mutant flies that are RNAi defective, demonstrating that successful infection of adult Drosophila requires a ..
  39. Pepper A, Beerman R, Bhogal B, Jongens T. Argonaute2 suppresses Drosophila fragile X expression preventing neurogenesis and oogenesis defects. PLoS ONE. 2009;4:e7618 pubmed publisher
    ..Over-expression of dFMR1 also causes specific neuronal and behavioral abnormalities. We have found that Argonaute2 (Ago2), the core component of the small interfering RNA (siRNA) pathway, regulates dfmr1 expression...
  40. Chotkowski H, Ciota A, Jia Y, Puig Basagoiti F, Kramer L, Shi P, et al. West Nile virus infection of Drosophila melanogaster induces a protective RNAi response. Virology. 2008;377:197-206 pubmed publisher
    ..RNAi knockdown of Argonaute 2 in S2 cells resulted in slightly increased susceptibility to WNV infection, suggesting that some WNV-homologous ..
  41. Rand T, Ginalski K, Grishin N, Wang X. Biochemical identification of Argonaute 2 as the sole protein required for RNA-induced silencing complex activity. Proc Natl Acad Sci U S A. 2004;101:14385-9 pubmed
    ..b>Argonaute 2 (Ago-2) is the sole protein component present in the purified, functional RISC...
  42. Li H, Li W, Ding S. Induction and suppression of RNA silencing by an animal virus. Science. 2002;296:1319-21 pubmed
    ..These findings establish RNA silencing as an adaptive antiviral defense in animal cells. B2 also inhibits RNA silencing in transgenic plants, providing evidence for a conserved RNA silencing pathway in the plant and animal kingdoms...
  43. Blumenstiel J, Fu R, Theurkauf W, Hawley R. Components of the RNAi machinery that mediate long-distance chromosomal associations are dispensable for meiotic and early somatic homolog pairing in Drosophila melanogaster. Genetics. 2008;180:1355-65 pubmed publisher
    ..Thus, there appears to be at least two mechanisms that bring homologous sequences together within the nucleus: those that act between dispersed homologous sequences and those that act to align and pair homologous chromosomes. ..
  44. Peng J, Karpen G. H3K9 methylation and RNA interference regulate nucleolar organization and repeated DNA stability. Nat Cell Biol. 2007;9:25-35 pubmed
    ..These results suggest a mechanism for how local chromatin structure can regulate genome stability, and the organization of chromosomal elements and nuclear organelles. ..
  45. Seitz H, Ghildiyal M, Zamore P. Argonaute loading improves the 5' precision of both MicroRNAs and their miRNA* strands in flies. Curr Biol. 2008;18:147-51 pubmed publisher
    ..Surprisingly, the precision of the 5' ends of both miRNA and miRNA* sequences increases after Argonaute2 (Ago2) loading...
  46. Okamura K, Ladewig E, Zhou L, Lai E. Functional small RNAs are generated from select miRNA hairpin loops in flies and mammals. Genes Dev. 2013;27:778-92 pubmed publisher
    ..60- to 70-nucleotide pre-miRNA hairpins that are cleaved by Dicer into small RNA duplexes that load into Argonaute proteins, which retain a single mature strand in the active complex...
  47. Moshkovich N, Nisha P, Boyle P, Thompson B, Dale R, Lei E. RNAi-independent role for Argonaute2 in CTCF/CP190 chromatin insulator function. Genes Dev. 2011;25:1686-701 pubmed publisher
    ..To address this question in Drosophila, we performed genome-wide localization of Argonaute2 (AGO2) by chromatin immunoprecipitation (ChIP)-seq in two different embryonic cell lines and found that AGO2 ..
  48. Czech B, Zhou R, Erlich Y, Brennecke J, Binari R, Villalta C, et al. Hierarchical rules for Argonaute loading in Drosophila. Mol Cell. 2009;36:445-56 pubmed publisher
    Drosophila Argonaute-1 and Argonaute-2 differ in function and small RNA content. AGO2 binds to siRNAs, whereas AGO1 is almost exclusively occupied by microRNAs...
  49. Hartig J, Esslinger S, Böttcher R, Saito K, Forstemann K. Endo-siRNAs depend on a new isoform of loquacious and target artificially introduced, high-copy sequences. EMBO J. 2009;28:2932-44 pubmed publisher
    ..One strand of the dsRNA precursor can come from spliced mRNA, whereas the opposite strand derives from independent transcripts in antisense orientation. ..
  50. Okamura K, Chung W, Ruby J, Guo H, Bartel D, Lai E. The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs. Nature. 2008;453:803-6 pubmed publisher
    ..on the fact that mutants lacking the core siRNA-generating enzyme Dicer-2 or the predominant siRNA effector Argonaute 2 are viable, fertile and of relatively normal morphology...
  51. Williams R, Rubin G. ARGONAUTE1 is required for efficient RNA interference in Drosophila embryos. Proc Natl Acad Sci U S A. 2002;99:6889-94 pubmed
    ..Notably, members of the Argonaute-like gene family from Arabidopsis, Caenorhabditis elegans, Drosophila, and Neurospora have been genetically and/..
  52. Bronkhorst A, van Cleef K, Vodovar N, Ince I, Blanc H, Vlak J, et al. The DNA virus Invertebrate iridescent virus 6 is a target of the Drosophila RNAi machinery. Proc Natl Acad Sci U S A. 2012;109:E3604-13 pubmed publisher
    ..We show that Dcr-2 and Argonaute-2 mutant flies are more sensitive to virus infection, suggesting that vsiRNAs contribute to the control of DNA ..
  53. Miyoshi K, Okada T, Siomi H, Siomi M. Characterization of the miRNA-RISC loading complex and miRNA-RISC formed in the Drosophila miRNA pathway. RNA. 2009;15:1282-91 pubmed publisher
    ..The AGO1-PIWI mutant showed no Slicer activity but associates with mature miRNA. These results indicate that these domains are required differently for miRLC and miRISC formation in the miRNA pathway. ..
  54. Harris A, Macdonald P. Aubergine encodes a Drosophila polar granule component required for pole cell formation and related to eIF2C. Development. 2001;128:2823-32 pubmed
    ..Unlike two other known polar granule components, Vasa and Oskar, Aubergine remains cytoplasmic after pole cell formation, suggesting that the roles of these proteins diverge during embryogenesis. ..
  55. Hammond S, Boettcher S, Caudy A, Kobayashi R, Hannon G. Argonaute2, a link between genetic and biochemical analyses of RNAi. Science. 2001;293:1146-50 pubmed
    ..Protein microsequencing reveals that one constituent of this complex is a member of the Argonaute family of proteins, which are essential for gene silencing in Caenorhabditis elegans, Neurospora, and ..
  56. Jiang F, Ye X, Liu X, Fincher L, McKearin D, Liu Q. Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila. Genes Dev. 2005;19:1674-9 pubmed
    ..Therefore, R3D1-L functions in concert with Dicer-1 in miRNA biogenesis and is required for reproductive development in Drosophila. ..
  57. Lingel A, Simon B, Izaurralde E, Sattler M. Structure and nucleic-acid binding of the Drosophila Argonaute 2 PAZ domain. Nature. 2003;426:465-9 pubmed
    ..The Argonaute 2 protein (Ago2) is a critical component of RISC...
  58. Li W, Li H, Lu R, Li F, Dus M, Atkinson P, et al. Interferon antagonist proteins of influenza and vaccinia viruses are suppressors of RNA silencing. Proc Natl Acad Sci U S A. 2004;101:1350-5 pubmed
    ..These findings provide evidence that mammalian virus proteins can inhibit RNA silencing, implicating this mechanism as a nucleic acid-based antiviral immunity in mammalian cells. ..
  59. Grewal S, Elgin S. Transcription and RNA interference in the formation of heterochromatin. Nature. 2007;447:399-406 pubmed
    ..Silencing of chromatin might involve trans-acting sources of the crucial small RNAs that carry out RNA interference, but in some cases, transcription of the region to be silenced seems to be required--an apparent contradiction. ..
  60. Meyer W, Schreiber S, Guo Y, Volkmann T, Welte M, Müller H. Overlapping functions of argonaute proteins in patterning and morphogenesis of Drosophila embryos. PLoS Genet. 2006;2:e134 pubmed
    b>Argonaute proteins are essential components of the molecular machinery that drives RNA silencing. In Drosophila, different members of the Argonaute family of proteins have been assigned to distinct RNA silencing pathways...
  61. Aliyari R, Wu Q, Li H, Wang X, Li F, Green L, et al. Mechanism of induction and suppression of antiviral immunity directed by virus-derived small RNAs in Drosophila. Cell Host Microbe. 2008;4:387-97 pubmed publisher
    ..the production by Dicer nuclease of virus-derived siRNAs (viRNAs), which guide specific antiviral silencing by Argonaute protein in an RNA-induced silencing complex (RISC)...
  62. Shah C, Forstemann K. Monitoring miRNA-mediated silencing in Drosophila melanogaster S2-cells. Biochim Biophys Acta. 2008;1779:766-72 pubmed publisher
    ..constructs and have examined the dependence of silencing by a particular Drosophila miRNA, bantam, on specific argonaute proteins...
  63. Caudy A, Myers M, Hannon G, Hammond S. Fragile X-related protein and VIG associate with the RNA interference machinery. Genes Dev. 2002;16:2491-6 pubmed
    ..The possibility that dFXR, and potentially FMRP, use, at least in part, an RNAi-related mechanism for target recognition suggests a potentially important link between RNAi and human disease. ..
  64. Obbard D, Jiggins F, Bradshaw N, Little T. Recent and recurrent selective sweeps of the antiviral RNAi gene Argonaute-2 in three species of Drosophila. Mol Biol Evol. 2011;28:1043-56 pubmed publisher
    ..The RNA interference pathway gene Argonaute-2 (AGO2) is a key component of antiviral defense in Drosophila, and we have previously shown that genes in this ..
  65. Han B, Hung J, Weng Z, Zamore P, Ameres S. The 3'-to-5' exoribonuclease Nibbler shapes the 3' ends of microRNAs bound to Drosophila Argonaute1. Curr Biol. 2011;21:1878-87 pubmed publisher
    ..miRNA duplexes then assemble into Argonaute proteins to form the RNA-induced silencing complex (RISC)...
  66. Miyoshi K, Tsukumo H, Nagami T, Siomi H, Siomi M. Slicer function of Drosophila Argonautes and its involvement in RISC formation. Genes Dev. 2005;19:2837-48 pubmed
    Argonaute proteins play important yet distinct roles in RNA silencing. Human Argonaute2 (hAgo2) was shown to be responsible for target RNA cleavage ("Slicer") activity in RNA interference (RNAi), whereas other Argonaute ..
  67. Monzo K, Papoulas O, Cantin G, Wang Y, Yates J, Sisson J. Fragile X mental retardation protein controls trailer hitch expression and cleavage furrow formation in Drosophila embryos. Proc Natl Acad Sci U S A. 2006;103:18160-5 pubmed
    ..Together, these data suggest that in cleavage-stage Drosophila embryos, dFMRP affects protein expression by controlling the availability and/or competency of specific transcripts to be translated. ..
  68. Xu K, Bogert B, Li W, Su K, Lee A, Gao F. The fragile X-related gene affects the crawling behavior of Drosophila larvae by regulating the mRNA level of the DEG/ENaC protein pickpocket1. Curr Biol. 2004;14:1025-34 pubmed
    ..DFMR1 downregulates the level of ppk1 mRNA in vivo, and this regulatory process also involves Argonaute2 (Ago2), a key component in the RNA interference pathway...
  69. Berezikov E, Robine N, Samsonova A, Westholm J, Naqvi A, Hung J, et al. Deep annotation of Drosophila melanogaster microRNAs yields insights into their processing, modification, and emergence. Genome Res. 2011;21:203-15 pubmed publisher
    ..libraries comprising diverse developmental stages, specific tissue- and cell-types, mutant conditions, and/or Argonaute immunoprecipitations...
  70. Ameres S, Hung J, Xu J, Weng Z, Zamore P. Target RNA-directed tailing and trimming purifies the sorting of endo-siRNAs between the two Drosophila Argonaute proteins. RNA. 2011;17:54-63 pubmed publisher
    ..not only their initial sorting between Ago1 and Ago2 according to their duplex structure, length, and first nucleotide, but also the targeted destruction of the single-stranded small RNAs after their loading into an Argonaute protein.
  71. Okamura K, Robine N, Liu Y, Liu Q, Lai E. R2D2 organizes small regulatory RNA pathways in Drosophila. Mol Cell Biol. 2011;31:884-96 pubmed publisher
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