Gene Symbol: AGO1
Description: Argonaute-1
Alias: AGO-1, Ago, Ago-1, Ago1, Agp1, CG6671, Dm Ago1, DmAgo1, Dmel\CG6671, MRE20, ago1, ago1-1, anon-WO0257455.29, dAGO1, dAgo1, l(2)04845, l(2)4845, l(2)k00208, l(2)k08121, Argonaute-1, AGO1-PA, AGO1-PB, AGO1-PC, AGO1-PD, Argonaute1, CG6671-PA, CG6671-PB, CG6671-PC, CG6671-PD, argonaute, argonaute 1, argonaute protein 1, argonaute-1, mRNA-like ncRNA in embryogenesis 20
Species: fruit fly
Products:     AGO1

Top Publications

  1. Grimaud C, Bantignies F, Pal Bhadra M, Ghana P, Bhadra U, Cavalli G. RNAi components are required for nuclear clustering of Polycomb group response elements. Cell. 2006;124:957-71 pubmed
    ..Dicer-2, PIWI, and Argonaute1, three RNAi components, frequently colocalize with PcG bodies, and their mutation significantly reduces the ..
  2. Kawamata T, Seitz H, Tomari Y. Structural determinants of miRNAs for RISC loading and slicer-independent unwinding. Nat Struct Mol Biol. 2009;16:953-60 pubmed publisher
    ..regulate expression of their target mRNAs through the RNA-induced silencing complex (RISC), which contains an Argonaute (Ago) family protein as a core component...
  3. Miyoshi K, Tsukumo H, Nagami T, Siomi H, Siomi M. Slicer function of Drosophila Argonautes and its involvement in RISC formation. Genes Dev. 2005;19:2837-48 pubmed
    b>Argonaute proteins play important yet distinct roles in RNA silencing...
  4. Kataoka Y, Takeichi M, Uemura T. Developmental roles and molecular characterization of a Drosophila homologue of Arabidopsis Argonaute1, the founder of a novel gene superfamily. Genes Cells. 2001;6:313-25 pubmed
    Arabidopsis Argonaute1 (AGO1) is the founder of a novel gene superfamily that is conserved from fission yeasts to humans. AGO1, and several other members of this superfamily are necessary for stem cell renewal or RNA interference...
  5. Tomari Y, Du T, Zamore P. Sorting of Drosophila small silencing RNAs. Cell. 2007;130:299-308 pubmed
    In Drosophila, small interfering RNAs (siRNAs), which direct RNA interference through the Argonaute protein Ago2, are produced by a biogenesis pathway distinct from microRNAs (miRNAs), which regulate endogenous mRNA expression as guides ..
  6. Yang L, Chen D, Duan R, Xia L, Wang J, Qurashi A, et al. Argonaute 1 regulates the fate of germline stem cells in Drosophila. Development. 2007;134:4265-72 pubmed
    ..Here, we show that overexpression of Argonaute 1 (AGO1) protein, another subfamily (AGO) of the Argonaute proteins, leads to GSC overproliferation, whereas loss ..
  7. Huntzinger E, Kuzuoğlu Öztürk D, Braun J, Eulalio A, Wohlbold L, Izaurralde E. The interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets. Nucleic Acids Res. 2013;41:978-94 pubmed publisher
    ..Silencing requires association of miRNAs with an Argonaute protein and a GW182 family protein...
  8. Jin P, Zarnescu D, Ceman S, Nakamoto M, Mowrey J, Jongens T, et al. Biochemical and genetic interaction between the fragile X mental retardation protein and the microRNA pathway. Nat Neurosci. 2004;7:113-7 pubmed
    ..with microRNAs and the components of the microRNA pathways including Dicer and the mammalian ortholog of Argonaute 1 (AGO1)...
  9. Diederichs S, Haber D. Dual role for argonautes in microRNA processing and posttranscriptional regulation of microRNA expression. Cell. 2007;131:1097-108 pubmed
    ..The miRNA is incorporated into the RNA-Induced Silencing Complex (RISC) with Argonaute proteins, the effector molecules in RNA interference (RNAi)...

More Information


  1. Haas G, Braun J, Igreja C, Tritschler F, Nishihara T, Izaurralde E. HPat provides a link between deadenylation and decapping in metazoa. J Cell Biol. 2010;189:289-302 pubmed publisher
  2. Ghildiyal M, Xu J, Seitz H, Weng Z, Zamore P. Sorting of Drosophila small silencing RNAs partitions microRNA* strands into the RNA interference pathway. RNA. 2010;16:43-56 pubmed publisher
    In flies, small silencing RNAs are sorted between Argonaute1 (Ago1), the central protein component of the microRNA (miRNA) pathway, and Argonaute2 (Ago2), which mediates RNA interference...
  3. Rehwinkel J, Behm Ansmant I, Gatfield D, Izaurralde E. A crucial role for GW182 and the DCP1:DCP2 decapping complex in miRNA-mediated gene silencing. RNA. 2005;11:1640-7 pubmed
    ..These include SMG5, SMG7, and UPF1, which function in nonsense-mediated mRNA decay (NMD), and the Argonaute proteins that are essential for RNA interference (RNAi) and the micro-RNA (miRNA) pathway...
  4. Tomari Y, Matranga C, Haley B, Martinez N, Zamore P. A protein sensor for siRNA asymmetry. Science. 2004;306:1377-80 pubmed
    ..protein heterodimer on the siRNA duplex determines which siRNA strand associates with the core RISC protein Argonaute 2...
  5. Okamura K, Ladewig E, Zhou L, Lai E. Functional small RNAs are generated from select miRNA hairpin loops in flies and mammals. Genes Dev. 2013;27:778-92 pubmed publisher
    ..60- to 70-nucleotide pre-miRNA hairpins that are cleaved by Dicer into small RNA duplexes that load into Argonaute proteins, which retain a single mature strand in the active complex...
  6. Kavi H, Fernandez H, Xie W, Birchler J. RNA silencing in Drosophila. FEBS Lett. 2005;579:5940-9 pubmed
    ..RNA silencing processes in Drosophila are described. ..
  7. Hammond S, Boettcher S, Caudy A, Kobayashi R, Hannon G. Argonaute2, a link between genetic and biochemical analyses of RNAi. Science. 2001;293:1146-50 pubmed
    ..Protein microsequencing reveals that one constituent of this complex is a member of the Argonaute family of proteins, which are essential for gene silencing in Caenorhabditis elegans, Neurospora, and ..
  8. Taliaferro J, Aspden J, Bradley T, Marwha D, Blanchette M, Rio D. Two new and distinct roles for Drosophila Argonaute-2 in the nucleus: alternative pre-mRNA splicing and transcriptional repression. Genes Dev. 2013;27:378-89 pubmed publisher
    ..Here we show that the small RNA pathway protein Argonaute-2 (Ago-2) regulates alternative pre-mRNA splicing patterns of specific transcripts in the Drosophila nucleus ..
  9. Moshkovich N, Lei E. HP1 recruitment in the absence of argonaute proteins in Drosophila. PLoS Genet. 2010;6:e1000880 pubmed publisher
    ..In order to determine if these Argonaute genes are required for heterochromatin formation, we utilized transcriptional reporters and chromatin ..
  10. Eulalio A, Helms S, Fritzsch C, Fauser M, Izaurralde E. A C-terminal silencing domain in GW182 is essential for miRNA function. RNA. 2009;15:1067-77 pubmed publisher
    Proteins of the GW182 family are essential for miRNA-mediated gene silencing in animal cells; they interact with Argonaute proteins (AGOs) and are required for both the translational repression and mRNA degradation mediated by miRNAs...
  11. Ameres S, Horwich M, Hung J, Xu J, Ghildiyal M, Weng Z, et al. Target RNA-directed trimming and tailing of small silencing RNAs. Science. 2010;328:1534-9 pubmed publisher
    In Drosophila, microRNAs (miRNAs) typically guide Argonaute1 to repress messenger RNA (mRNA), whereas small interfering RNAs (siRNAs) guide Argonaute2 to destroy viral and transposon RNA...
  12. Li C, Vagin V, Lee S, Xu J, Ma S, Xi H, et al. Collapse of germline piRNAs in the absence of Argonaute3 reveals somatic piRNAs in flies. Cell. 2009;137:509-21 pubmed publisher
    ..Transposons targeted by this second pathway often reside in the flamenco locus, which is expressed in somatic ovarian follicle cells, suggesting a role for piRNAs beyond the germline. ..
  13. Nayak A, Berry B, Tassetto M, Kunitomi M, Acevedo A, Deng C, et al. Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila. Nat Struct Mol Biol. 2010;17:547-54 pubmed publisher
    ..In contrast, the CrPV suppressor (CrPV-1A) interacts with the endonuclease Argonaute 2 (Ago2) and inhibits its activity without affecting the microRNA (miRNA)-Ago1-mediated silencing...
  14. Zekri L, Huntzinger E, Heimstädt S, Izaurralde E. The silencing domain of GW182 interacts with PABPC1 to promote translational repression and degradation of microRNA targets and is required for target release. Mol Cell Biol. 2009;29:6220-31 pubmed publisher
    ..Previous studies showed that while the GW182 N-terminal domain interacts with Argonaute proteins, translational repression and degradation of miRNA targets are promoted by a bipartite silencing domain ..
  15. Ni J, Zhou R, Czech B, Liu L, Holderbaum L, Yang Zhou D, et al. A genome-scale shRNA resource for transgenic RNAi in Drosophila. Nat Methods. 2011;8:405-7 pubmed publisher
    ..We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource. ..
  16. Meyer W, Schreiber S, Guo Y, Volkmann T, Welte M, Müller H. Overlapping functions of argonaute proteins in patterning and morphogenesis of Drosophila embryos. PLoS Genet. 2006;2:e134 pubmed
    b>Argonaute proteins are essential components of the molecular machinery that drives RNA silencing. In Drosophila, different members of the Argonaute family of proteins have been assigned to distinct RNA silencing pathways...
  17. Moretti F, Kaiser C, Zdanowicz Specht A, Hentze M. PABP and the poly(A) tail augment microRNA repression by facilitated miRISC binding. Nat Struct Mol Biol. 2012;19:603-8 pubmed publisher
    ..We integrated these data into a revised model for the function of PABP and the poly(A) tail in miRNA-mediated translational repression. ..
  18. Abe M, Naqvi A, Hendriks G, Feltzin V, Zhu Y, Grigoriev A, et al. Impact of age-associated increase in 2'-O-methylation of miRNAs on aging and neurodegeneration in Drosophila. Genes Dev. 2014;28:44-57 pubmed publisher
    ..Small RNA deep sequencing revealed a global increase of miRNAs loaded into Ago2, but not into Ago1, with age...
  19. Gunawardane L, Saito K, Nishida K, Miyoshi K, Kawamura Y, Nagami T, et al. A slicer-mediated mechanism for repeat-associated siRNA 5' end formation in Drosophila. Science. 2007;315:1587-90 pubmed
    ..These data support a model in which formation of a 5' terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with the Slicer activity of PIWI. ..
  20. Harris A, Macdonald P. Aubergine encodes a Drosophila polar granule component required for pole cell formation and related to eIF2C. Development. 2001;128:2823-32 pubmed
    ..Unlike two other known polar granule components, Vasa and Oskar, Aubergine remains cytoplasmic after pole cell formation, suggesting that the roles of these proteins diverge during embryogenesis. ..
  21. Nishida K, Saito K, Mori T, Kawamura Y, Nagami Okada T, Inagaki S, et al. Gene silencing mechanisms mediated by Aubergine piRNA complexes in Drosophila male gonad. RNA. 2007;13:1911-22 pubmed
    ..These results provide the first biochemical insights into gene silencing mechanisms mediated by Aub and piRNAs in fly testes. ..
  22. Brennecke J, Aravin A, Stark A, Dus M, Kellis M, Sachidanandam R, et al. Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila. Cell. 2007;128:1089-103 pubmed
    ..Thus, sense piRNAs, formed following cleavage of transposon mRNAs may enhance production of antisense piRNAs, complementary to active elements, by directing cleavage of transcripts from master control loci. ..
  23. Iwasaki S, Kawamata T, Tomari Y. Drosophila argonaute1 and argonaute2 employ distinct mechanisms for translational repression. Mol Cell. 2009;34:58-67 pubmed publisher
    ..have established an in vitro system that recapitulates translational repression mediated by the two Drosophila Argonaute (Ago) subfamily proteins, Ago1 and Ago2...
  24. Aliyari R, Wu Q, Li H, Wang X, Li F, Green L, et al. Mechanism of induction and suppression of antiviral immunity directed by virus-derived small RNAs in Drosophila. Cell Host Microbe. 2008;4:387-97 pubmed publisher
    ..the production by Dicer nuclease of virus-derived siRNAs (viRNAs), which guide specific antiviral silencing by Argonaute protein in an RNA-induced silencing complex (RISC)...
  25. Findley S, Tamanaha M, Clegg N, Ruohola Baker H. Maelstrom, a Drosophila spindle-class gene, encodes a protein that colocalizes with Vasa and RDE1/AGO1 homolog, Aubergine, in nuage. Development. 2003;130:859-71 pubmed
    ..Furthermore, maelstrom mutant ovaries show mislocalization of two proteins involved in the microRNA and/or RNAi pathways, Dicer and Argonaute2, suggesting a potential connection between nuage and the microRNA-pathway. ..
  26. Horwich M, Li C, Matranga C, Vagin V, Farley G, Wang P, et al. The Drosophila RNA methyltransferase, DmHen1, modifies germline piRNAs and single-stranded siRNAs in RISC. Curr Biol. 2007;17:1265-72 pubmed
    ..2'-O-methylation of siRNAs may be the final step in assembly of the RNAi-enzyme complex, RISC, occurring after an Argonaute-bound siRNA duplex is converted to single-stranded RNA.
  27. Backes S, Shapiro J, Sabin L, Pham A, Reyes I, Moss B, et al. Degradation of host microRNAs by poxvirus poly(A) polymerase reveals terminal RNA methylation as a protective antiviral mechanism. Cell Host Microbe. 2012;12:200-10 pubmed publisher
    ..These findings suggest that poxviruses may degrade host miRNAs to promote replication and that virus-mediated small RNA degradation likely contributed to 2'OMe evolution. ..
  28. Djuranovic S, Nahvi A, Green R. miRNA-mediated gene silencing by translational repression followed by mRNA deadenylation and decay. Science. 2012;336:237-40 pubmed publisher
    ..We next used a natural translational elongation stall to show that miRNA-mediated silencing inhibits translation at an early step, potentially translation initiation. ..
  29. Zekri L, Kuzuoğlu Öztürk D, Izaurralde E. GW182 proteins cause PABP dissociation from silenced miRNA targets in the absence of deadenylation. EMBO J. 2013;32:1052-65 pubmed publisher
    GW182 family proteins interact with Argonaute proteins and are required for the translational repression, deadenylation and decay of miRNA targets...
  30. Saito K, Sakaguchi Y, Suzuki T, Suzuki T, Siomi H, Siomi M. Pimet, the Drosophila homolog of HEN1, mediates 2'-O-methylation of Piwi- interacting RNAs at their 3' ends. Genes Dev. 2007;21:1603-8 pubmed
    ..Recombinant Pimet shows single-stranded small RNA methylation activity in vitro and interacts with the PIWI proteins within Pimet mutant ovary. These results show that Pimet mediates piRNA 2'-O-methylation in Drosophila. ..
  31. Haley B, Hendrix D, Trang V, Levine M. A simplified miRNA-based gene silencing method for Drosophila melanogaster. Dev Biol. 2008;321:482-90 pubmed publisher
    ..As proof of concept, this method was used for the efficient silencing of dpp gene activity in the adult wing, and the analysis of the general RNA Polymerase II (Pol II) elongation factor, Nelf-E. ..
  32. Pradhan S, Nesler K, Rosen S, Kato Y, Nakamura A, Ramaswami M, et al. The conserved P body component HPat/Pat1 negatively regulates synaptic terminal growth at the larval Drosophila neuromuscular junction. J Cell Sci. 2012;125:6105-16 pubmed publisher
    ..interacts genetically with the catalytic subunit of the deadenylase complex (twin/CCR4) and the miRNA pathway (Argonaute 1) to control bouton formation...
  33. Till S, Lejeune E, Thermann R, Bortfeld M, Hothorn M, Enderle D, et al. A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. Nat Struct Mol Biol. 2007;14:897-903 pubmed
    b>Argonaute (Ago) proteins mediate silencing of nucleic acid targets by small RNAs. In fission yeast, Ago1, Tas3 and Chp1 assemble into a RITS complex, which silences transcription near centromeres...
  34. Li Y, Maines J, Tastan O, McKearin D, Buszczak M. Mei-P26 regulates the maintenance of ovarian germline stem cells by promoting BMP signaling. Development. 2012;139:1547-56 pubmed publisher
    ..These data suggest that Mei-P26 has distinct functions in the ovary and participates in regulating the fates of both GSCs and their differentiating daughters. ..
  35. Chung W, Okamura K, Martin R, Lai E. Endogenous RNA interference provides a somatic defense against Drosophila transposons. Curr Biol. 2008;18:795-802 pubmed publisher
    ..small interfering RNAs (siRNAs), which are 21 nucleotide, 3'-end-modified RNAs that are dependent on Dicer-2 and Argonaute-2...
  36. Czech B, Zhou R, Erlich Y, Brennecke J, Binari R, Villalta C, et al. Hierarchical rules for Argonaute loading in Drosophila. Mol Cell. 2009;36:445-56 pubmed publisher
    Drosophila Argonaute-1 and Argonaute-2 differ in function and small RNA content. AGO2 binds to siRNAs, whereas AGO1 is almost exclusively occupied by microRNAs...
  37. Miyoshi K, Okada T, Siomi H, Siomi M. Characterization of the miRNA-RISC loading complex and miRNA-RISC formed in the Drosophila miRNA pathway. RNA. 2009;15:1282-91 pubmed publisher
    In Drosophila, miRNA is processed by Dicer-1 (DCR-1) from its precursor and loaded onto Argonaute1 (AGO1). AGO1 recognizes target mRNAs based on the miRNA sequence and suppresses the expression at post-transcriptional levels...
  38. Ameres S, Hung J, Xu J, Weng Z, Zamore P. Target RNA-directed tailing and trimming purifies the sorting of endo-siRNAs between the two Drosophila Argonaute proteins. RNA. 2011;17:54-63 pubmed publisher
    ..flies, 22-23-nucleotide (nt) microRNA duplexes typically contain mismatches and begin with uridine, so they bind Argonaute1 (Ago1), whereas 21-nt siRNA duplexes are perfectly paired and begin with cytidine, promoting their loading into ..
  39. Eulalio A, Rehwinkel J, Stricker M, Huntzinger E, Yang S, Doerks T, et al. Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing. Genes Dev. 2007;21:2558-70 pubmed
    ..In addition to proteins known to be required for miRNA biogenesis and function (i.e., Drosha, Pasha, Dicer-1, AGO1, and GW182), the screen identified the decapping activator Ge-1 as being required for silencing by miRNAs...
  40. Eulalio A, Huntzinger E, Izaurralde E. GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay. Nat Struct Mol Biol. 2008;15:346-53 pubmed publisher
    ..In contrast, silencing is abolished by mutating Argonaute 1 (AGO1) at two conserved phenylalanine residues predicted to mediate binding to the cap structure...
  41. Jäger E, Dorner S. The decapping activator HPat a novel factor co-purifying with GW182 from Drosophila cells. RNA Biol. 2010;7:381-5 pubmed
    ..Our findings implicate a potential interaction of the miRNA effector component GW182 with the decapping machinery. ..
  42. Kibanov M, Egorova K, Ryazansky S, Sokolova O, Kotov A, Olenkina O, et al. A novel organelle, the piNG-body, in the nuage of Drosophila male germ cells is associated with piRNA-mediated gene silencing. Mol Biol Cell. 2011;22:3410-9 pubmed publisher
    ..body contains known ovarian nuage proteins, including Vasa, Aub, AGO3, Tud, Spn-E, Bel, Squ, and Cuff, as well as AGO1, the key component of the microRNA pathway...
  43. Miyoshi K, Miyoshi T, Hartig J, Siomi H, Siomi M. Molecular mechanisms that funnel RNA precursors into endogenous small-interfering RNA and microRNA biogenesis pathways in Drosophila. RNA. 2010;16:506-15 pubmed publisher
    ..Interestingly, however, small RNAs derived from terminal hairpins of esiRNA precursors are loaded onto AGO1; thus, they are classified as a new subset of miRNAs...
  44. Preston C, Sved J, Engels W. Flanking duplications and deletions associated with P-induced male recombination in Drosophila. Genetics. 1996;144:1623-38 pubmed
    ..Furthermore, the retention of a mobile P element allows one to extend the deletion or generate additional variability at the site by subsequent rounds of recombination. ..
  45. Berdnik D, Fan A, Potter C, Luo L. MicroRNA processing pathway regulates olfactory neuron morphogenesis. Curr Biol. 2008;18:1754-9 pubmed publisher
    ..However, Argonaute-1 and Argonaute-2 are dispensable for PN morphogenesis...
  46. Grewal S, Elgin S. Transcription and RNA interference in the formation of heterochromatin. Nature. 2007;447:399-406 pubmed
    ..Silencing of chromatin might involve trans-acting sources of the crucial small RNAs that carry out RNA interference, but in some cases, transcription of the region to be silenced seems to be required--an apparent contradiction. ..
  47. Okamura K, Hagen J, Duan H, Tyler D, Lai E. The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila. Cell. 2007;130:89-100 pubmed
    ..and seed-matched targets, and we provide evidence that they function, at least in part, via the RNA-induced silencing complex effector Ago1. These findings reveal that mirtrons are an alternate source of miRNA-type regulatory RNAs.
  48. Kadener S, Menet J, Sugino K, Horwich M, Weissbein U, Nawathean P, et al. A role for microRNAs in the Drosophila circadian clock. Genes Dev. 2009;23:2179-91 pubmed publisher
    ..To identify miRNA-mRNA pairs important for this regulation, immunoprecipitation of AGO1 followed by microarray analysis identified mRNAs under miRNA-mediated control...
  49. Vagin V, Sigova A, Li C, Seitz H, Gvozdev V, Zamore P. A distinct small RNA pathway silences selfish genetic elements in the germline. Science. 2006;313:320-4 pubmed
    ..Unlike siRNAs and miRNAs, rasiRNAs function through the Piwi, rather than the Ago, Argonaute protein subfamily...
  50. Tomari Y, Zamore P. Perspective: machines for RNAi. Genes Dev. 2005;19:517-29 pubmed
    ..Here, we review our current understanding of how small RNAs are produced, how they are loaded into protein complexes, and how they repress gene expression. ..
  51. Iwasaki S, Kobayashi M, Yoda M, Sakaguchi Y, Katsuma S, Suzuki T, et al. Hsc70/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes. Mol Cell. 2010;39:292-9 pubmed publisher
    ..Both siRNAs and miRNAs are born double stranded. Surprisingly, loading these small RNA duplexes into Argonaute proteins, the core components of RISC, requires ATP, whereas separating the two small RNA strands within ..
  52. Forstemann K, Horwich M, Wee L, Tomari Y, Zamore P. Drosophila microRNAs are sorted into functionally distinct argonaute complexes after production by dicer-1. Cell. 2007;130:287-97 pubmed
    ..Thus, the two members of the Drosophila Ago subclade of Argonaute proteins are functionally specialized, but specific small RNA classes are not restricted to associate with Ago1 ..
  53. Okamura K, Phillips M, Tyler D, Duan H, Chou Y, Lai E. The regulatory activity of microRNA* species has substantial influence on microRNA and 3' UTR evolution. Nat Struct Mol Biol. 2008;15:354-63 pubmed publisher
    ..abundant than their partners, they are often present at physiologically relevant levels and can associate with Argonaute proteins...
  54. Caudy A, Myers M, Hannon G, Hammond S. Fragile X-related protein and VIG associate with the RNA interference machinery. Genes Dev. 2002;16:2491-6 pubmed
    ..The possibility that dFXR, and potentially FMRP, use, at least in part, an RNAi-related mechanism for target recognition suggests a potentially important link between RNAi and human disease. ..
  55. Fukaya T, Tomari Y. MicroRNAs mediate gene silencing via multiple different pathways in drosophila. Mol Cell. 2012;48:825-36 pubmed publisher
    MicroRNAs (miRNAs) guide RNA-induced silencing complex (RISC) that contains an Argonaute family protein to complementary target messenger RNAs (mRNAs)...
  56. Han B, Hung J, Weng Z, Zamore P, Ameres S. The 3'-to-5' exoribonuclease Nibbler shapes the 3' ends of microRNAs bound to Drosophila Argonaute1. Curr Biol. 2011;21:1878-87 pubmed publisher
    ..Here, we show that after loading into Argonaute1 (Ago1), more than a quarter of all Drosophila miRNAs undergo 3' end trimming by the 3'-to-5' exoribonuclease ..
  57. Okamura K, Robine N, Liu Y, Liu Q, Lai E. R2D2 organizes small regulatory RNA pathways in Drosophila. Mol Cell Biol. 2011;31:884-96 pubmed publisher
    ..siRNAs) are generally produced by different Dicer enzymes (Dcr-1 and Dcr-2) and sorted to functionally distinct Argonaute effectors (AGO1 and AGO2)...
  58. Hartig J, Esslinger S, Böttcher R, Saito K, Forstemann K. Endo-siRNAs depend on a new isoform of loquacious and target artificially introduced, high-copy sequences. EMBO J. 2009;28:2932-44 pubmed publisher
    ..One strand of the dsRNA precursor can come from spliced mRNA, whereas the opposite strand derives from independent transcripts in antisense orientation. ..
  59. Chekulaeva M, Mathys H, Zipprich J, Attig J, Colic M, Parker R, et al. miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs. Nat Struct Mol Biol. 2011;18:1218-26 pubmed publisher
    ..GW182 proteins are recruited to the miRNA repression complex through direct interaction with Argonaute proteins, and they function downstream to repress target mRNA...
  60. Leuschner P, Obernosterer G, Martinez J. MicroRNAs: Loquacious speaks out. Curr Biol. 2005;15:R603-5 pubmed
    ..New data show that Dicer-1 also requires a double-stranded RNA binding protein called Loquacious for efficient microRNA-mediated gene silencing. ..
  61. Okamura K, Liu N, Lai E. Distinct mechanisms for microRNA strand selection by Drosophila Argonautes. Mol Cell. 2009;36:431-44 pubmed publisher
    In Drosophila, miRNA strands are predominantly sorted into AGO1 to regulate seed-matched target transcripts, while their partner miRNA* strands are thought to be mostly degraded...
  62. Neumüller R, Betschinger J, Fischer A, Bushati N, Poernbacher I, Mechtler K, et al. Mei-P26 regulates microRNAs and cell growth in the Drosophila ovarian stem cell lineage. Nature. 2008;454:241-5 pubmed publisher
    ..We identify Argonaute-1, a component of the RISC complex, as a common binding partner of Brat and Mei-P26, and show that Mei-P26 acts ..
  63. Eulalio A, Tritschler F, Büttner R, Weichenrieder O, Izaurralde E, Truffault V. The RRM domain in GW182 proteins contributes to miRNA-mediated gene silencing. Nucleic Acids Res. 2009;37:2974-83 pubmed publisher
    Proteins of the GW182 family interact with Argonaute proteins and are required for miRNA-mediated gene silencing...
  64. Eulalio A, Huntzinger E, Nishihara T, Rehwinkel J, Fauser M, Izaurralde E. Deadenylation is a widespread effect of miRNA regulation. RNA. 2009;15:21-32 pubmed publisher
    ..melanogaster cells depleted of AGO1, CAF1, or NOT1...
  65. Pressman S, Reinke C, Wang X, Carthew R. A Systematic Genetic Screen to Dissect the MicroRNA Pathway in Drosophila. G3 (Bethesda). 2012;2:437-48 pubmed publisher
    ..4 previously known genes that encode core components of the miRNA pathway, including Drosha, Pasha, Dicer-1, and Ago1. The rest are new genes that function through chromatin remodeling, signaling, and mRNA decapping...
  66. Xu X, Li Y, Wang F, Gao F. The steady-state level of the nervous-system-specific microRNA-124a is regulated by dFMR1 in Drosophila. J Neurosci. 2008;28:11883-9 pubmed publisher
    ..This effect could be explained in part by the reduced abundance of the Dicer-1-Ago1 complex in the absence of dFMR1...
  67. Herranz H, Hong X, Perez L, Ferreira A, Olivieri D, Cohen S, et al. The miRNA machinery targets Mei-P26 and regulates Myc protein levels in the Drosophila wing. EMBO J. 2010;29:1688-98 pubmed publisher
    ..Thus, regulation of tissue growth by the miRNA pathway involves a double repression mechanism to control dMyc protein levels in a highly proliferative and growing epithelium. ..
  68. Smibert P, Yang J, Azzam G, Liu J, Lai E. Homeostatic control of Argonaute stability by microRNA availability. Nat Struct Mol Biol. 2013;20:789-95 pubmed publisher
    ..mammalian systems to demonstrate a conserved homeostatic system in which the status of miRNA biogenesis controls Argonaute protein stability...
  69. Nishida K, Miyoshi K, Ogino A, Miyoshi T, Siomi H, Siomi M. Roles of R2D2, a cytoplasmic D2 body component, in the endogenous siRNA pathway in Drosophila. Mol Cell. 2013;49:680-91 pubmed publisher
    ..In r2d2 mutants, the level of endo-siRNAs is unchanged, but endo-siRNAs are misloaded onto Ago1. However, the mechanism underlying the control of endo-siRNA sorting by R2D2 remains unknown...
  70. Shah C, Forstemann K. Monitoring miRNA-mediated silencing in Drosophila melanogaster S2-cells. Biochim Biophys Acta. 2008;1779:766-72 pubmed publisher
    ..constructs and have examined the dependence of silencing by a particular Drosophila miRNA, bantam, on specific argonaute proteins...
  71. Obbard D, Jiggins F, Halligan D, Little T. Natural selection drives extremely rapid evolution in antiviral RNAi genes. Curr Biol. 2006;16:580-5 pubmed
    ..This is a signature of host-pathogen arms races and implies that the ancient battle between RNA viruses and host antiviral RNAi genes is active and significant in shaping RNAi function. ..
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