Gene Symbol: Act87E
Description: Actin 87E
Alias: Act87e, Actin, Actin87E, ArpC, CG18290, Dmel\CG18290, act 87E, act87E, actin, mus87E, actin 87E, Act87E-PA, Act87E-PB, Act87E-PC, CG18290-PA, CG18290-PB, CG18290-PC, actin, actin87E
Species: fruit fly
Products:     Act87E

Top Publications

  1. Bai J, Binari R, Ni J, Vijayakanthan M, Li H, Perrimon N. RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance. Development. 2008;135:1439-49 pubmed publisher
    ..Finally, we discuss how Drosophila primary cells can be manipulated to develop cell-based assays to model human diseases for RNAi and small-molecule screens. ..
  2. Bai J, Hartwig J, Perrimon N. SALS, a WH2-domain-containing protein, promotes sarcomeric actin filament elongation from pointed ends during Drosophila muscle growth. Dev Cell. 2007;13:828-42 pubmed
    Organization of actin filaments into a well-organized sarcomere structure is critical for muscle development and function. However, it is not completely understood how sarcomeric actin/thin filaments attain their stereotyped lengths...
  3. Röper K, Mao Y, Brown N. Contribution of sequence variation in Drosophila actins to their incorporation into actin-based structures in vivo. J Cell Sci. 2005;118:3937-48 pubmed
    b>Actin is a highly conserved protein important for many cellular functions including motility, contraction in muscles and intracellular transport...
  4. Barbosa V, Gatt M, Rebollo E, Gonzalez C, Glover D. Drosophila dd4 mutants reveal that gammaTuRC is required to maintain juxtaposed half spindles in spermatocytes. J Cell Sci. 2003;116:929-41 pubmed
    ..We conclude that spermatocytes do not need astral microtubules nucleated by opposite poles to intersect in order to form a central spindle and a cleavage furrow. ..
  5. Riparbelli M, Callaini G, Glover D, Avides M. A requirement for the Abnormal Spindle protein to organise microtubules of the central spindle for cytokinesis in Drosophila. J Cell Sci. 2002;115:913-22 pubmed
    ..Upon fertilisation, Asp is also recruited to the MTOC that nucleates the sperm aster. Asp is required for growth of the microtubules of the sperm aster, which in asp mutants remains diminutive and so prevents migration of the pronuclei. ..
  6. Mounier N, Gouy M, Mouchiroud D, Prudhomme J. Insect muscle actins differ distinctly from invertebrate and vertebrate cytoplasmic actins. J Mol Evol. 1992;34:406-15 pubmed
    ..However, Bombyx and Drosophila have actin genes specifically expressed in muscles...
  7. Röper K, Brown N. Maintaining epithelial integrity: a function for gigantic spectraplakin isoforms in adherens junctions. J Cell Biol. 2003;162:1305-15 pubmed
    ..Previously characterized Shot isoforms are similar to spectrin and dystrophin, with an actin-binding domain followed by spectrin repeats...
  8. Eltsov M, Dubé N, Yu Z, Pasakarnis L, Haselmann Weiss U, Brunner D, et al. Quantitative analysis of cytoskeletal reorganization during epithelial tissue sealing by large-volume electron tomography. Nat Cell Biol. 2015;17:605-14 pubmed publisher
    ..After establishing contact through actin-driven exploratory filopodia, cells use a single lamella to generate 'roof tile'-like overlaps...
  9. Shah A, Nongthomba U, Kelly Tanaka K, Denton M, Meadows S, Bancroft N, et al. Cardiac remodeling in Drosophila arises from changes in actin gene expression and from a contribution of lymph gland-like cells to the heart musculature. Mech Dev. 2011;128:222-33 pubmed publisher
    ..In addition, expression of the Actin87E actin gene is initiated in the remodeling cardiac tube, but not in the longitudinal fibers, and we have ..

More Information


  1. Drosopoulou E, Tsiafouli M, Mavragani Tsipidou P, Scouras Z. The glutamate dehydrogenase, E74 and putative actin gene loci in the Drosophila montium subgroup. Chromosomal homologies among the montium species and D. melanogaster. Chromosoma. 1997;106:20-8 pubmed
    ..from an enzyme-coding gene (glutamate dehydrogenase, gdh), a regulatory protein-coding gene (E74) and genes of the actin family were mapped by in situ hybridization on the polytene chromosomes of six species representative of the ..
  2. Lenartowska M, Isaji M, Miller K. A pre-embedding immunogold approach reveals localization of myosin VI at the ultrastructural level in the actin cones that mediate Drosophila spermatid individualization. Protoplasma. 2012;249:337-46 pubmed publisher
    Stable actin structures play important roles in the development and specialization of differentiated cells. How these structures form, are organized, and are used to mediate physiological processes is not well understood in most cases...
  3. Adams J, Kelso R, Cooley L. The kelch repeat superfamily of proteins: propellers of cell function. Trends Cell Biol. 2000;10:17-24 pubmed
    ..In this review, we discuss current information concerning the structural organization of kelch repeat proteins, their biological roles and the molecular basis of their action. ..
  4. Fyrberg E, Davidson N, Kindle K. The actin genes of Drosophila: a dispersed multigene family. Cell. 1980;19:365-78 pubmed
    We have initiated a study of the organization and expression of the actin genes of D. melanogaster...
  5. Fyrberg E, Fyrberg C, Biggs J, Saville D, Beall C, Ketchum A. Functional nonequivalence of Drosophila actin isoforms. Biochem Genet. 1998;36:271-87 pubmed
    We show that different Drosophila actin isoforms are not interchangeable. We sequenced the six genes that encode conventional Drosophila actins and found that they specify amino acid replacements in 27 of 376 positions...
  6. Bearer E. Actin in the Drosophila embryo: is there a relationship to developmental cue localization?. Bioessays. 1991;13:199-204 pubmed
    ..The actin-based cytoskeleton is a likely candidate for the formation of a cytomatrix that would allow such distributions and ..
  7. Manseau L, Ganetzky B, Craig E. Molecular and genetic characterization of the Drosophila melanogaster 87E actin gene region. Genetics. 1988;119:407-20 pubmed
    A combined molecular and genetic analysis of the 87E actin gene (Act87E) in Drosophila melanogaster was undertaken. A clone of Act87E was isolated and characterized. The Act87E transcription unit is 1...
  8. Baehrecke E. Caspase activation finds fertile ground. Dev Cell. 2003;4:608-9 pubmed
    ..Recent work demonstrates that cytochrome c and caspases function in Drosophila sperm cell differentiation and indicates that caspase activity can be regulated in a subcellular manner in cells that live. ..
  9. He M, Haymer D. Codon bias in actin multigene families and effects on the reconstruction of phylogenetic relationships. J Mol Evol. 1995;41:141-9 pubmed
    Codon usage patterns and phylogenetic relationships in the actin multigene family have been analyzed for three dipteran species--Drosophila melanogaster, Bactrocera dorsalis, and Ceratitis capitata...
  10. Schmitz S, Clayton J, Nongthomba U, Prinz H, Veigel C, Geeves M, et al. Drosophila ACT88F indirect flight muscle-specific actin is not N-terminally acetylated: a mutation in N-terminal processing affects actin function. J Mol Biol. 2000;295:1201-10 pubmed
    ..In class II actins, which include muscle actins, the common primary sequence of Met-Cys-Asp-actin is processed to acetyl-Asp-actin. The functional significance of this in vivo is unknown...
  11. Tobin S, Cook P, Burn T. Transcripts of individual Drosophila actin genes are differentially distributed during embryogenesis. Dev Genet. 1990;11:15-26 pubmed
    The temporal and spatial patterns of accumulation of transcripts from individual actin genes during Drosophila embryogenesis have been determined by in situ hybridization...
  12. Wang Y, Duttke S, Chen K, Johnston J, Kassavetis G, Zeitlinger J, et al. TRF2, but not TBP, mediates the transcription of ribosomal protein genes. Genes Dev. 2014;28:1550-5 pubmed publisher
    ..Hence, a specialized TRF2-based RNA polymerase II system functions in the synthesis of RPs and complements the RNA polymerase I and III systems. ..
  13. Nongthomba U, Pasalodos Sanchez S, Clark S, Clayton J, Sparrow J. Expression and function of the Drosophila ACT88F actin isoform is not restricted to the indirect flight muscles. J Muscle Res Cell Motil. 2001;22:111-9 pubmed
    Most higher eukaryotic genomes contain multiple actin genes, yet the sequence differences between isoforms are few...
  14. Hongo S, Jacobs Lorena M. Translational discrimination of ribosomal protein mRNAs in the early Drosophila embryo. Dev Biol. 1991;145:338-43 pubmed
    ..No evidence for involvement of reversibly binding trans-acting factors was obtained, although there are limitations in the interpretation of the latter experiments. ..
  15. García Zaragoza E, Mas J, Vivar J, Arredondo J, Cervera M. CF2 activity and enhancer integration are required for proper muscle gene expression in Drosophila. Mech Dev. 2008;125:617-30 pubmed publisher
    ..However, it may also be a more general mechanism to control the correct levels of gene expression during development in each cell type. ..
  16. Tilney L, Derosier D. How to make a curved Drosophila bristle using straight actin bundles. Proc Natl Acad Sci U S A. 2005;102:18785-92 pubmed
    This, our Inaugural Article as Academy Members, is ironically our swan song from the field of the actin cytoskeleton...
  17. Fyrberg E, Bond B, Hershey N, Mixter K, Davidson N. The actin genes of Drosophila: protein coding regions are highly conserved but intron positions are not. Cell. 1981;24:107-16 pubmed
    The entire set of six closely related Drosophila actin genes was isolated using recombinant DNA methodology, and the structures of the respective coding regions were characterized by gene mapping techniques and by nucleotide sequencing ..
  18. Belozerov V, Ratkovic S, McNeill H, Hilliker A, McDermott J. In vivo interaction proteomics reveal a novel p38 mitogen-activated protein kinase/Rack1 pathway regulating proteostasis in Drosophila muscle. Mol Cell Biol. 2014;34:474-84 pubmed publisher
    ..Biochemical analyses of Rack1 in aging and stressed muscle suggest a model whereby p38 MAPK signaling causes a redistribution of Rack1 between a ribosome-bound pool and a putative translational repressor complex. ..
  19. Kusch T, Florens L, Macdonald W, Swanson S, Glaser R, Yates J, et al. Acetylation by Tip60 is required for selective histone variant exchange at DNA lesions. Science. 2004;306:2084-7 pubmed
    ..Thus, these data reveal a previously unknown mechanism for selective histone exchange that uses the concerted action of two distinct chromatin-remodeling enzymes within the same multiprotein complex. ..
  20. White M, Crother B. Diagnostic amino acids in actin genes: an idea whose time has gone. Mol Biol Evol. 1999;16:876-9 pubmed
  21. Baum B. Winging it--actin on the fly. Dev Cell. 2002;2:125-6 pubmed
    How actin dynamics might be regulated to generate specific cellular structures during development remains something of a mystery...
  22. Miller K. A role for moesin in polarity. Trends Cell Biol. 2003;13:165-8 pubmed
    ..Previously, studies in cultured mammalian cells suggested that ERM proteins are important for actin-membrane associations...
  23. Strickland L, Burgess D. Pathways for membrane trafficking during cytokinesis. Trends Cell Biol. 2004;14:115-8 pubmed
    ..Furthermore, new work reveals that vesicles derived from the Rab11 recycling endosome (RE) promote actin remodeling at the furrow.
  24. Aoki T, Sarkeshik A, Yates J, Schedl P. Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complex. elife. 2012;1:e00171 pubmed publisher
    ..We show that Elba is an unusual hetero-tripartite protein complex that requires all three proteins for DNA binding and insulator activity.DOI: ..
  25. Jaiswal M, Agrawal N, Sinha P. Fat and Wingless signaling oppositely regulate epithelial cell-cell adhesion and distal wing development in Drosophila. Development. 2006;133:925-35 pubmed
    ..Finally, we show that Ft intersects Wnt/Wg signaling, downstream of the Wg ligand. Fat and Wg signaling thus exert opposing regulation to coordinate cell-cell adhesion and patterning along the PD axis of Drosophila wing. ..
  26. Razzaq A, Schmitz S, Veigel C, Molloy J, Geeves M, Sparrow J. Actin residue glu(93) is identified as an amino acid affecting myosin binding. J Biol Chem. 1999;274:28321-8 pubmed
    Many mutants have been described that affect the function of the actin encoded by the Drosophila melanogaster indirect flight muscle-specific actin gene, Act88F...
  27. Fernandes I, Schöck F. The nebulin repeat protein Lasp regulates I-band architecture and filament spacing in myofibrils. J Cell Biol. 2014;206:559-72 pubmed publisher
    Mutations in nebulin, a giant muscle protein with 185 actin-binding nebulin repeats, are the major cause of nemaline myopathy in humans...
  28. Jaiswal R, Stepanik V, Rankova A, Molinar O, Goode B, McCartney B. Drosophila homologues of adenomatous polyposis coli (APC) and the formin diaphanous collaborate by a conserved mechanism to stimulate actin filament assembly. J Biol Chem. 2013;288:13897-905 pubmed publisher
    Vertebrate APC collaborates with Dia through its Basic domain to assemble actin filaments...
  29. Biggin M. To bind or not to bind. Nat Genet. 2001;28:303-4 pubmed
    ..Genome-wide surveys of DNA binding by transcription factors in vivo are beginning to provide some answers. ..
  30. Inoue Y, Savoian M, Suzuki T, Mathe E, Yamamoto M, Glover D. Mutations in orbit/mast reveal that the central spindle is comprised of two microtubule populations, those that initiate cleavage and those that propagate furrow ingression. J Cell Biol. 2004;166:49-60 pubmed
    ..orbit mutants have disorganized or incomplete anillin and actin rings, and although cleavage furrows initiate, they ultimately regress...
  31. Raisner R, Madhani H. Patterning chromatin: form and function for H2A.Z variant nucleosomes. Curr Opin Genet Dev. 2006;16:119-24 pubmed
    ..This chromatin pattern is generated through the action of a DNA deposition signal and a specific pattern of histone tail acetylation. ..
  32. Bucciarelli E, Giansanti M, Bonaccorsi S, Gatti M. Spindle assembly and cytokinesis in the absence of chromosomes during Drosophila male meiosis. J Cell Biol. 2003;160:993-9 pubmed
    ..This suggests that the association of Aurora B with chromosomes is not a prerequisite for its accumulation at the central spindle, or for its function during cytokinesis. ..
  33. D Avino P, Takeda T, Capalbo L, Zhang W, Lilley K, Laue E, et al. Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site. J Cell Sci. 2008;121:1151-8 pubmed publisher
    Anillin, one of the first factors recruited to the cleavage site during cytokinesis, interacts with actin, myosin II and septins, and is essential for proper organization of the actomyosin contractile ring...
  34. Ma L, Ma J, Xu K. Effect of spaceflight on the circadian rhythm, lifespan and gene expression of Drosophila melanogaster. PLoS ONE. 2015;10:e0121600 pubmed publisher
  35. Padash Barmchi M, Rogers S, Hacker U. DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo. J Cell Biol. 2005;168:575-85 pubmed
    Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization of the actin cytoskeleton that is mediated by small GTPases of the Rho family...
  36. Gelbart M, Larschan E, Peng S, Park P, Kuroda M. Drosophila MSL complex globally acetylates H4K16 on the male X chromosome for dosage compensation. Nat Struct Mol Biol. 2009;16:825-32 pubmed publisher
  37. Tobin S, Zulauf E, Sanchez F, Craig E, McCarthy B. Multiple actin-related sequences in the Drosophila melanogaster genome. Cell. 1980;19:121-31 pubmed
    ..melanogaster chromosomal DNA fragment (K1) which contains nucleotide sequences complementary to D. melanogaster actin mRNA. Plasmid K1 was identified using a Dictyostelium actin cDNA plasmid, B1, as a probe. D...
  38. Rui Y, Bai J, Perrimon N. Sarcomere formation occurs by the assembly of multiple latent protein complexes. PLoS Genet. 2010;6:e1001208 pubmed publisher
    ..Thus, sarcomere formation occurs by the coordinated assembly of multiple latent protein complexes, as opposed to sequential assembly. ..
  39. Zeitler J, Hsu C, Dionne H, Bilder D. Domains controlling cell polarity and proliferation in the Drosophila tumor suppressor Scribble. J Cell Biol. 2004;167:1137-46 pubmed
    ..We suggest a model in which Scrib, via the activity of the LRR, governs proliferation primarily by regulating apicobasal polarity. ..
  40. Fyrberg E, Mahaffey J, Bond B, Davidson N. Transcripts of the six Drosophila actin genes accumulate in a stage- and tissue-specific manner. Cell. 1983;33:115-23 pubmed
    ..Act57A and act87E are expressed predominantly in larval, pupal, and adult intersegmental muscles; act88F in muscles of the adult ..
  41. Lo S. Focal adhesions: what's new inside. Dev Biol. 2006;294:280-91 pubmed
    ..adhesions is comprised of large molecular complexes that link transmembrane receptors, such as integrins, to the actin cytoskeleton and mediate signals modulating cell attachment, migration, proliferation, differentiation, and gene ..
  42. Labuhn M, Brack C. Age-related changes in the mRNA expression of actin isoforms in Drosophila melanogaster. Gerontology. 1997;43:261-7 pubmed
    ..Here we have examined age-related changes in the mRNA levels of the gene family encoding the six Drosophila actin genes...
  43. Davis J, Luchici A, Mosis F, Thackery J, Salazar J, Mao Y, et al. Inter-cellular forces orchestrate contact inhibition of locomotion. Cell. 2015;161:361-73 pubmed publisher
    ..Tracking actin retrograde flow within hemocytes in vivo reveals synchronous reorganization of colliding actin networks through ..
  44. Mounier N, Sparrow J. Muscle actin genes in insects. Comp Biochem Physiol B. 1993;105:231-8 pubmed