flhA

Summary

Gene Symbol: flhA
Description: predicted flagellar export pore protein
Alias: ECK1880, JW1868, flaH
Species:

Top Publications

  1. ncbi Interactions of FliJ with the Salmonella type III flagellar export apparatus
    Gillian M Fraser
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Bacteriol 185:5546-54. 2003
  2. ncbi Domain movements of HAP2 in the cap-filament complex formation and growth process of the bacterial flagellum
    Saori Maki-Yonekura
    Protonic NanoMachine Project, Exploratory Research for Advanced Technology, Japan Science and Technology Agency, 3 4 Hikaridai, Seika, Kyoto 619 0237, Japan
    Proc Natl Acad Sci U S A 100:15528-33. 2003
  3. ncbi Docking of cytosolic chaperone-substrate complexes at the membrane ATPase during flagellar type III protein export
    Joanne Thomas
    Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
    Proc Natl Acad Sci U S A 101:3945-50. 2004
  4. ncbi Domain organization and function of Salmonella FliK, a flagellar hook-length control protein
    Tohru Minamino
    Dynamic NanoMachine Project, ICORP, JST, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Mol Biol 341:491-502. 2004
  5. ncbi Analysis of the cytoplasmic domains of Salmonella FlhA and interactions with components of the flagellar export machinery
    Jonathan L McMurry
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    J Bacteriol 186:7586-92. 2004
  6. ncbi N-terminal signal region of FliK is dispensable for length control of the flagellar hook
    Takanori Hirano
    CREST, Japan Science and Technology Agency JST, 1064 18 Takahori, Hirata, Takanezawa, Shioya gun, Tochigi 329 1206, Japan
    Mol Microbiol 56:346-60. 2005
  7. ncbi FlhB regulates ordered export of flagellar components via autocleavage mechanism
    Hedda U Ferris
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Biol Chem 280:41236-42. 2005
  8. ncbi Crystallization and preliminary X-ray analysis of the C-terminal cytoplasmic domain of FlhA, a membrane-protein subunit of the bacterial flagellar type III protein-export apparatus
    Yumiko Saijo-Hamano
    Dynamic NanoMachine Project, ICORP, JST, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:599-602. 2005
  9. ncbi The type III flagellar export specificity switch is dependent on FliK ruler and a molecular clock
    Nao Moriya
    Graduate School of Frontier Biosciences, Osaka University, Osaka 565 0871, Japan
    J Mol Biol 359:466-77. 2006
  10. ncbi Interactions between C ring proteins and export apparatus components: a possible mechanism for facilitating type III protein export
    Bertha González-Pedrajo
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    Mol Microbiol 60:984-98. 2006

Research Grants

  1. Protein Interactions in the Flagellar Export Machinery
    JONATHAN MCMURRY; Fiscal Year: 2005
  2. Purification of a Modified Flagellar Export Apparatus
    JONATHAN MCMURRY; Fiscal Year: 2007

Scientific Experts

  • Tohru Minamino
  • R M Macnab
  • Hedda U Ferris
  • Jonathan L McMurry
  • Bertha González-Pedrajo
  • Keiichi Namba
  • Gillian M Fraser
  • Nao Moriya
  • May Kihara
  • Yumiko Saijo-Hamano
  • Takanori Hirano
  • Joanne Thomas
  • Colin Hughes
  • Saori Maki-Yonekura
  • Laurent Claret
  • Keng Zhu
  • M Kihara
  • T Kubori
  • Kelly T Hughes
  • S Maki
  • K Namba
  • K Hasegawa
  • Tomomi Tani
  • Satoshi Shibata
  • Kouhei Ohnishi
  • Katsumi Imada
  • Shin Ichi Aizawa
  • Shin-Ichi Aizawa
  • Graham P Stafford
  • Koji Yonekura
  • Susannah R Calder
  • Matthew Higgins
  • Jeremy R H Tame
  • F Fan
  • S Yamaguchi
  • J E Galan
  • A Sukhan
  • S I Aizawa
  • Y Furukawa
  • K Kutsukake
  • I Yamashita
  • K Imada
  • F Vonderviszt
  • T Yokoseki

Detail Information

Publications29

  1. ncbi Interactions of FliJ with the Salmonella type III flagellar export apparatus
    Gillian M Fraser
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Bacteriol 185:5546-54. 2003
    ..that FliJ interacts with the soluble cytoplasmic domain of the largest membrane component of the export apparatus, FlhA; although small deletions in FliJ did not interfere with the association, both truncated versions failed to ..
  2. ncbi Domain movements of HAP2 in the cap-filament complex formation and growth process of the bacterial flagellum
    Saori Maki-Yonekura
    Protonic NanoMachine Project, Exploratory Research for Advanced Technology, Japan Science and Technology Agency, 3 4 Hikaridai, Seika, Kyoto 619 0237, Japan
    Proc Natl Acad Sci U S A 100:15528-33. 2003
    ....
  3. ncbi Docking of cytosolic chaperone-substrate complexes at the membrane ATPase during flagellar type III protein export
    Joanne Thomas
    Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
    Proc Natl Acad Sci U S A 101:3945-50. 2004
    ..Membrane localization persisted in fliOPQR, flhB, flhA, fliJ, and fliH null mutants lacking specific flagellar export components but depended on the presence of the ..
  4. ncbi Domain organization and function of Salmonella FliK, a flagellar hook-length control protein
    Tohru Minamino
    Dynamic NanoMachine Project, ICORP, JST, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Mol Biol 341:491-502. 2004
    ..The conformational flexibility of FliK(C) may play a role in interfering with switching at an inappropriate point of flagellar assembly...
  5. ncbi Analysis of the cytoplasmic domains of Salmonella FlhA and interactions with components of the flagellar export machinery
    Jonathan L McMurry
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    J Bacteriol 186:7586-92. 2004
    ..flagellar proteins are exported via a type III export apparatus which, in part, consists of the membrane proteins FlhA, FlhB, FliO, FliP, FliQ, and FliR and is housed within the membrane-supramembrane ring formed by FliF subunits...
  6. ncbi N-terminal signal region of FliK is dispensable for length control of the flagellar hook
    Takanori Hirano
    CREST, Japan Science and Technology Agency JST, 1064 18 Takahori, Hirata, Takanezawa, Shioya gun, Tochigi 329 1206, Japan
    Mol Microbiol 56:346-60. 2005
    ....
  7. ncbi FlhB regulates ordered export of flagellar components via autocleavage mechanism
    Hedda U Ferris
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Biol Chem 280:41236-42. 2005
    ..Finally, we provide evidence via peptide analysis and FlhB cleavage variants that the tertiary structure of FlhB plays a significant role in cleavage. Based on these results, we propose that FlhB cleavage is an autocatalytic process...
  8. ncbi Crystallization and preliminary X-ray analysis of the C-terminal cytoplasmic domain of FlhA, a membrane-protein subunit of the bacterial flagellar type III protein-export apparatus
    Yumiko Saijo-Hamano
    Dynamic NanoMachine Project, ICORP, JST, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:599-602. 2005
    ..b>FlhA is an essential component of the type III export apparatus of Salmonella and consists of two major portions: an N-..
  9. ncbi The type III flagellar export specificity switch is dependent on FliK ruler and a molecular clock
    Nao Moriya
    Graduate School of Frontier Biosciences, Osaka University, Osaka 565 0871, Japan
    J Mol Biol 359:466-77. 2006
    ..We propose that FliK(N) acts as a flexible tape measure, but that hook length is also dependent on the hook elongation rate and a switch timing mechanism...
  10. ncbi Interactions between C ring proteins and export apparatus components: a possible mechanism for facilitating type III protein export
    Bertha González-Pedrajo
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    Mol Microbiol 60:984-98. 2006
    ..The importance of the C ring as a possible docking site for export substrates, chaperones and FliI through FliH for their efficient delivery to membrane components of the export apparatus is discussed...
  11. ncbi Oligomerization of the bacterial flagellar ATPase FliI is controlled by its extreme N-terminal region
    Tohru Minamino
    Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Mol Biol 360:510-9. 2006
    ....
  12. ncbi Two parts of the T3S4 domain of the hook-length control protein FliK are essential for the substrate specificity switching of the flagellar type III export apparatus
    Tohru Minamino
    Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Mol Biol 362:1148-58. 2006
    ....
  13. ncbi The ATPase FliI can interact with the type III flagellar protein export apparatus in the absence of its regulator, FliH
    Tohru Minamino
    Protonic NanoMachine Project, ERATO and Dynamic NanoMachine Project, ICORP, JST, Seika, Kyoto 619 0237, Japan
    J Bacteriol 185:3983-8. 2003
    ..Mutations in the cytoplasmic domains of FlhA and FlhB, which are integral membrane components of the type III flagellar export apparatus, also resulted in ..
  14. ncbi Oligomerization and activation of the FliI ATPase central to bacterial flagellum assembly
    Laurent Claret
    Cambridge University, Department of Pathology, Tennis Court Road, UK
    Mol Microbiol 48:1349-55. 2003
    ..The data reveal central facets of the structure and action of the flagellar assembly ATPase and, by extension, the homologous ATPases of virulence-related type III export systems...
  15. ncbi Molecular dissection of Salmonella FliH, a regulator of the ATPase FliI and the type III flagellar protein export pathway
    Bertha González-Pedrajo
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    Mol Microbiol 45:967-82. 2002
    ..Based on these data, a model is presented for the domain construction and function of FliH in complex with FliI and FliJ...
  16. ncbi Isolation and characterization of FliK-independent flagellation mutants from Salmonella typhimurium
    K Kutsukake
    Faculty of Applied Biological Science, Hiroshima University, Japan
    J Bacteriol 176:7625-9. 1994
    ..On the basis of these results, we discuss the mechanism of suppression of the fliK defects by the flhB mutations and propose a hypothesis on the export switching machinery of the flagellar proteins...
  17. ncbi Enzymatic characterization of FliI. An ATPase involved in flagellar assembly in Salmonella typhimurium
    F Fan
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Biol Chem 271:31981-8. 1996
    ..We propose that FliI has a C-terminal ATPase domain and an N-terminal domain that interacts with other components in the flagellum-specific export apparatus...
  18. ncbi Quasi- and nonequivalence in the structure of bacterial flagellar filament
    K Hasegawa
    International Institute for Advanced Research, Matsushita Electric Industrial Company, Seika, Japan
    Biophys J 74:569-75. 1998
    ....
  19. ncbi Plugging interactions of HAP2 pentamer into the distal end of flagellar filament revealed by electron microscopy
    S Maki
    International Institute for Advanced Research, Matsushita Electric Industrial Co, Ltd, 3 4 Hikaridai, Seika, 619 02, Japan
    J Mol Biol 277:771-7. 1998
    ..This also allows us to model the axial domain arrangement of flagellin subunit in the filament...
  20. ncbi Components of the Salmonella flagellar export apparatus and classification of export substrates
    T Minamino
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Bacteriol 181:1388-94. 1999
    ..Hook-capping protein (FlgD) and hook protein (FlgE) required FlhA, FlhB, FliH, FliI, FliO, FliP, FliQ, and FliR for their export to the periplasm...
  21. ncbi Interactions among components of the Salmonella flagellar export apparatus and its substrates
    T Minamino
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Mol Microbiol 35:1052-64. 2000
    ..We propose a model for the flagellar export apparatus in which FlhA and FlhB and the other four integral membrane proteins of the apparatus form a complex at the base of the flagellar ..
  22. ncbi Role of FliJ in flagellar protein export in Salmonella
    T Minamino
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Bacteriol 182:4207-15. 2000
    ..We conclude that FliJ is a general component of the flagellar export apparatus and has a chaperone-like activity for both rod/hook-type and filament-type substrates...
  23. ncbi Domain structure of Salmonella FlhB, a flagellar export component responsible for substrate specificity switching
    T Minamino
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Bacteriol 182:4906-14. 2000
    ..FliK itself is an export substrate; its binding properties for FlhB(C) resemble those of FlgD and do not provide any evidence for a physical interaction beyond that of the export process...
  24. ncbi Molecular characterization and assembly of the needle complex of the Salmonella typhimurium type III protein secretion system
    T Kubori
    Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT 06536, USA
    Proc Natl Acad Sci U S A 97:10225-30. 2000
    ..We hypothesize that the needle component may establish the specificity of type III secretion systems in delivering proteins into either plant or animal cells...
  25. ncbi FliH, a soluble component of the type III flagellar export apparatus of Salmonella, forms a complex with FliI and inhibits its ATPase activity
    T Minamino
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Mol Microbiol 37:1494-503. 2000
    ....
  26. ncbi Intergenic suppression between the flagellar MS ring protein FliF of Salmonella and FlhA, a membrane component of its export apparatus
    M Kihara
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Bacteriol 183:1655-62. 2001
    ..The 19 intergenic suppressors identified all lay in flhA, and they consisted of 10 independent examples at the nucleotide level or 9 at the amino acid level...
  27. ncbi Interactions among membrane and soluble components of the flagellar export apparatus of Salmonella
    Keng Zhu
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    Biochemistry 41:9516-24. 2002
    ..The components examined were two integral membrane proteins, FlhA and FlhB, and two soluble components, FliH and the ATPase FliI...
  28. ncbi The FliN-FliH interaction mediates localization of flagellar export ATPase FliI to the C ring complex
    Jonathan L McMurry
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    Biochemistry 45:11790-8. 2006
    ..A model incorporating the present findings is presented...

Research Grants2

  1. Protein Interactions in the Flagellar Export Machinery
    JONATHAN MCMURRY; Fiscal Year: 2005
    ..biophysical analysis of interactions with analytical ultracentrifugation and fluorescence resonance energy transfer studies; and reconstitution of complexes of export apparatus proteins and analysis of their concerted function. ..
  2. Purification of a Modified Flagellar Export Apparatus
    JONATHAN MCMURRY; Fiscal Year: 2007
    ..Construction and purification of a modified export apparatus and studies of the dynamics of apparatus and substrate interactions will lead to an enhanced understanding of this primary mechanism of bacterial pathogenesis. ..