Gene Symbol: ubi4
Description: ubiquitin
Alias: ubiquitin
Species: fission yeast

Top Publications

  1. Nakase M, Nakase Y, Chardwiriyapreecha S, Kakinuma Y, Matsumoto T, Takegawa K. Intracellular trafficking and ubiquitination of the Schizosaccharomyces pombe amino acid permease Aat1p. Microbiology. 2012;158:659-73 pubmed publisher
    ..S. pombe has three genes (pub1(+), pub2(+) and pub3(+)) that are homologous to the ubiquitin ligase RSP5...
  2. Okazaki K, Okayama H, Niwa O. The polyubiquitin gene is essential for meiosis in fission yeast. Exp Cell Res. 2000;254:143-52 pubmed
    ..The ubi4(+) mRNA was strongly induced for meiosis. However, ubiquitin increases only slightly, suggesting that the role of the polyubiquitin gene is to supply ubiquitin that is ..
  3. Fang Y, Jaiseng W, Ma Y, Hu L, Yamazaki S, Zhang X, et al. E3 ubiquitin ligase Pub1 is implicated in endocytosis of a GPI-anchored protein Ecm33 in fission yeast. PLoS ONE. 2014;9:e85238 pubmed publisher
    ..Here, we further identified two multicopy suppressor genes, ubi1 (+) and ubc4 (+), encoding ubiquitin-ribosomal fusion protein and ubiquitin conjugating enzyme E2, respectively...
  4. Penney M, Samejima I, Wilkinson C, McInerny C, Mathiassen S, Wallace M, et al. Fission yeast 26S proteasome mutants are multi-drug resistant due to stabilization of the Pap1 transcription factor. PLoS ONE. 2012;7:e50796 pubmed publisher
    ..We show that the ubiquitylation and ultimately the degradation of Pap1 depend on the Rhp6/Ubc2 E2 ubiquitin conjugating enzyme and the Ubr1 E3 ubiquitin-protein ligase...
  5. Medina B, Paraskevopoulos K, Boehringer J, Sznajder A, Robertson M, Endicott J, et al. The ubiquitin-associated (UBA) 1 domain of Schizosaccharomyces pombe Rhp23 is essential for the recognition of ubiquitin-proteasome system substrates both in vitro and in vivo. J Biol Chem. 2012;287:42344-51 pubmed publisher
    The ubiquitin-proteasome system is essential for maintaining a functional cell. Not only does it remove incorrectly folded proteins, it also regulates protein levels to ensure their appropriate spatial and temporal distribution...
  6. Nakashima A, Kamada S, Tamanoi F, Kikkawa U. Fission yeast arrestin-related trafficking adaptor, Arn1/Any1, is ubiquitinated by Pub1 E3 ligase and regulates endocytosis of Cat1 amino acid transporter. Biol Open. 2014;3:542-52 pubmed publisher
    ..Arn1 interacts with and is ubiquitinated by the Pub1 ubiquitin ligase, which is necessary to regulate Cat1 endocytosis...
  7. Andersen K, Semple C, Hartmann Petersen R. Characterisation of the nascent polypeptide-associated complex in fission yeast. Mol Biol Rep. 2007;34:275-81 pubmed
    ..The NAC alpha subunit contains a ubiquitin-associated (UBA) domain, which is found in several proteins involved in the ubiquitin-proteasome pathway for ..
  8. Harrison C, Katayama S, Dhut S, Chen D, Jones N, Bahler J, et al. SCF(Pof1)-ubiquitin and its target Zip1 transcription factor mediate cadmium response in fission yeast. EMBO J. 2005;24:599-610 pubmed
    b>Ubiquitin-dependent proteolysis regulates gene expression in many eukaryotic systems. Pof1 is an essential fission yeast F-box protein that is homologous to budding yeast Met30...
  9. Baber Furnari B, Rhind N, Boddy M, Shanahan P, Lopez Girona A, Russell P. Regulation of mitotic inhibitor Mik1 helps to enforce the DNA damage checkpoint. Mol Biol Cell. 2000;11:1-11 pubmed
    ..Coordinated negative regulation of Cdc25 and positive regulation of Mik1 ensure the effective operation of the DNA damage checkpoint. ..

More Information


  1. Ramasubramanyan S, Coulon S, Fuchs R, Lehmann A, Green C. Ubiquitin-PCNA fusion as a mimic for mono-ubiquitinated PCNA in Schizosaccharomyces pombe. DNA Repair (Amst). 2010;9:777-84 pubmed publisher
    ..Mono-ubiquitinated PCNA is a key regulator of this process. We have investigated whether a ubiquitin-PCNA fusion can mimic ubiquitinated PCNA, by transforming plasmids expressing this fusion protein into different ..
  2. Weikart N, Sommer S, Mootz H. Click synthesis of ubiquitin dimer analogs to interrogate linkage-specific UBA domain binding. Chem Commun (Camb). 2012;48:296-8 pubmed publisher
    A new route to the synthesis of triazole-linked ubiquitin dimers (diUbs) as structural analogs of the seven diUbs is reported...
  3. Kominami K, Toda T. Fission yeast WD-repeat protein pop1 regulates genome ploidy through ubiquitin-proteasome-mediated degradation of the CDK inhibitor Rum1 and the S-phase initiator Cdc18. Genes Dev. 1997;11:1548-60 pubmed
    ..that it belongs to a WD-repeat family with highest homology to budding yeast Cdc4, which participates in the ubiquitin-dependent pathway...
  4. Horikoshi Y, Habu T, Matsumoto T. An E2 enzyme Ubc11 is required for ubiquitination of Slp1/Cdc20 and spindle checkpoint silencing in fission yeast. Cell Cycle. 2013;12:961-71 pubmed publisher
    For ordered mitotic progression, various proteins have to be regulated by an ubiquitin ligase, the anaphase-promoting complex or cyclosome (APC/C) with appropriate timing...
  5. LLOYD S, Raychaudhuri S, Espenshade P. Subunit architecture of the Golgi Dsc E3 ligase required for sterol regulatory element-binding protein (SREBP) cleavage in fission yeast. J Biol Chem. 2013;288:21043-54 pubmed publisher
    ..Further characterization of Dsc2 revealed that its C-terminal UBA domain can bind to ubiquitin chains but that the Dsc2 UBA domain is not essential for yeast SREBP cleavage...
  6. Otsubo Y, Yamashita A, Ohno H, Yamamoto M. S. pombe TORC1 activates the ubiquitin-proteasomal degradation of the meiotic regulator Mei2 in cooperation with Pat1 kinase. J Cell Sci. 2014;127:2639-46 pubmed publisher
    ..Based on these data, we propose that TORC1 contributes to the suppression of sexual development by phosphorylating Mei2, in addition to controlling the cellular metabolic status. ..
  7. Frampton J, Irmisch A, Green C, Neiss A, Trickey M, Ulrich H, et al. Postreplication repair and PCNA modification in Schizosaccharomyces pombe. Mol Biol Cell. 2006;17:2976-85 pubmed
    ..We show that PCNA modification and cell cycle checkpoints represent two independent signals in response to DNA damage. Finally, we unexpectedly find that PCNA is ubiquitinated in response to DNA damage when cells are arrested in G2. ..
  8. Olsen S, Lima C. Structure of a ubiquitin E1-E2 complex: insights to E1-E2 thioester transfer. Mol Cell. 2013;49:884-96 pubmed publisher
    b>Ubiquitin (Ub) conjugation is initiated by an E1 enzyme that catalyzes carboxy-terminal Ub adenylation, thioester bond formation to a catalytic cysteine in the E1 Cys domain, and thioester transfer to a catalytic cysteine in E2 ..
  9. Kitamura K, Katayama S, Dhut S, Sato M, Watanabe Y, Yamamoto M, et al. Phosphorylation of Mei2 and Ste11 by Pat1 kinase inhibits sexual differentiation via ubiquitin proteolysis and 14-3-3 protein in fission yeast. Dev Cell. 2001;1:389-99 pubmed
    ..Here, we show how Pat1 downregulates these proteins. Mei2 is degraded via a ubiquitin-proteasome pathway in a phosphorylation-dependent fashion...
  10. Gonzalez M, He H, Dong Q, Sun S, Li F. Ectopic centromere nucleation by CENP--a in fission yeast. Genetics. 2014;198:1433-46 pubmed publisher
    ..sites and provide evidence that the N-terminal domain of CENP-A prevents CENP-A assembly at ectopic loci via the ubiquitin-dependent proteolysis...
  11. Paraskevopoulos K, Kriegenburg F, Tatham M, Rösner H, Medina B, Larsen I, et al. Dss1 is a 26S proteasome ubiquitin receptor. Mol Cell. 2014;56:453-61 pubmed publisher
    The ubiquitin-proteasome system is the major pathway for protein degradation in eukaryotic cells. Proteins to be degraded are conjugated to ubiquitin chains that act as recognition signals for the 26S proteasome...