slp1

Summary

Gene Symbol: slp1
Description: WD repeat-containing protein
Alias: cdc20, WD repeat-containing protein
Species: fission yeast

Top Publications

  1. Kim S, Lin D, Matsumoto S, Kitazono A, Matsumoto T. Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint. Science. 1998;279:1045-7 pubmed
    ..Mad2 formed a complex with Slp1, a WD (tryptophan-aspartic acid)-repeat protein essential for the onset of anaphase...
  2. Matsumoto T. A fission yeast homolog of CDC20/p55CDC/Fizzy is required for recovery from DNA damage and genetically interacts with p34cdc2. Mol Cell Biol. 1997;17:742-50 pubmed
    ..of slp1+ revealed that slp1+ encodes a putative 488-amino-acid polypeptide exhibiting significant homology to WD-domain proteins, namely, CDC20 (budding yeast), p55CDC (human), and Fizzy (fly). A possible role of slp1+ is proposed.
  3. Izawa D, Goto M, Yamashita A, Yamano H, Yamamoto M. Fission yeast Mes1p ensures the onset of meiosis II by blocking degradation of cyclin Cdc13p. Nature. 2005;434:529-33 pubmed
    ..We therefore propose that Mes1p has a key function in saving a sufficient level of MPF (M-phase-promoting factor) activity required for the execution of MII. ..
  4. Millband D, Hardwick K. Fission yeast Mad3p is required for Mad2p to inhibit the anaphase-promoting complex and localizes to kinetochores in a Bub1p-, Bub3p-, and Mph1p-dependent manner. Mol Cell Biol. 2002;22:2728-42 pubmed
    ..Mad3p coimmunoprecipitates Bub3p, Mad2p, and the spindle checkpoint effector Slp1/Cdc20p...
  5. Sczaniecka M, Feoktistova A, May K, Chen J, Blyth J, Gould K, et al. The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C). J Biol Chem. 2008;283:23039-47 pubmed publisher
    ..The APC/C requires activators (Cdc20 or Cdh1) to efficiently recognize its substrates, which are specified by destruction (D box) and/or KEN box ..
  6. Ikui A, Furuya K, Yanagida M, Matsumoto T. Control of localization of a spindle checkpoint protein, Mad2, in fission yeast. J Cell Sci. 2002;115:1603-10 pubmed
    ..Mad2, a component of the checkpoint, targets fission yeast Slp1/budding yeast Cdc20/human p55CDC and prevents it from promoting proteolysis, which is a prerequisite to sister chromatid separation...
  7. Singh G, Karade S, Ranjan R, Ahamad N, Ahmed S. C-terminal region of Mad2 plays an important role during mitotic spindle checkpoint in fission yeast Schizosaccharomyces pombe. Mol Biol Rep. 2017;44:89-96 pubmed publisher
    ..deficiency 2 (Mad2) protein is an essential component of the spindle assembly checkpoint that interacts with Cdc20/Slp1 and inhibit its ability to activate anaphase promoting complex/cyclosome (APC/C)...
  8. Ito D, Saito Y, Matsumoto T. Centromere-tethered Mps1 pombe homolog (Mph1) kinase is a sufficient marker for recruitment of the spindle checkpoint protein Bub1, but not Mad1. Proc Natl Acad Sci U S A. 2012;109:209-14 pubmed publisher
    ..We propose that Mph1 is a sufficient marker for recruitment of Bub1. Mad1, in contrast, requires an additional condition/component for stable association with kinetochores. ..
  9. Horikoshi Y, Habu T, Matsumoto T. An E2 enzyme Ubc11 is required for ubiquitination of Slp1/Cdc20 and spindle checkpoint silencing in fission yeast. Cell Cycle. 2013;12:961-71 pubmed publisher
    ..that the activity of APC/C also contributes to release of mitotic checkpoint complexes (MCCs) from its target Cdc20 in the process of silencing the spindle assembly checkpoint (SAC)...

More Information

Publications18

  1. Chikashige Y, Yamane M, Okamasa K, Osakada H, Tsutsumi C, Nagahama Y, et al. Fission yeast APC/C activators Slp1 and Fzr1 sequentially trigger two consecutive nuclear divisions during meiosis. FEBS Lett. 2017;591:1029-1040 pubmed publisher
    ..In this mutant, we found that the initiation codon of the slp1+ gene is converted to ATA, producing a reduced amount of Slp1...
  2. Sewart K, Hauf S. Different Functionality of Cdc20 Binding Sites within the Mitotic Checkpoint Complex. Curr Biol. 2017;27:1213-1220 pubmed publisher
    ..Checkpoint signaling produces the "mitotic checkpoint complex" (MCC), which prevents anaphase by targeting Cdc20, the activator of the anaphase-promoting complex/cyclosome (APC/C)...
  3. Matsumura T, Yuasa T, Hayashi T, Obara T, Kimata Y, Yanagida M. A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids. Genes Cells. 2003;8:341-55 pubmed
    ..In addition, the manner of the inhibition by high copy securin and separase plasmids were quite different each other and did not result in the mitotic block. ..
  4. Sugimoto I, Murakami H, Tonami Y, Moriyama A, Nakanishi M. DNA replication checkpoint control mediated by the spindle checkpoint protein Mad2p in fission yeast. J Biol Chem. 2004;279:47372-8 pubmed
    ..These results suggested that Mad2p and Slp1p act downstream of Rad3p in the DNA replication checkpoint and that Mad2p is required for the DNA replication checkpoint when Cds1p is compromised. ..
  5. Tatebe H, Yanagida M. Cut8, essential for anaphase, controls localization of 26S proteasome, facilitating destruction of cyclin and Cut2. Curr Biol. 2000;10:1329-38 pubmed
    ..Cut8 is a novel evolutionarily conserved heat-inducible regulator. It facilitates anaphase-promoting proteolysis by recruiting 26S proteasome to a functionally efficient nuclear location. ..
  6. Yamada H, Matsumoto S, Matsumoto T. High dosage expression of a zinc finger protein, Grt1, suppresses a mutant of fission yeast slp1(+), a homolog of CDC20/p55CDC/Fizzy. J Cell Sci. 2000;113 ( Pt 22):3989-99 pubmed
    ..It requires ubiquitination of substrates by Anaphase Promoting Complex(APC)/Cyclosome. Slp1, a WD-repeat protein, is a putative activator for APC in fission yeast...
  7. Kimata Y, Kitamura K, Fenner N, Yamano H. Mes1 controls the meiosis I to meiosis II transition by distinctly regulating the anaphase-promoting complex/cyclosome coactivators Fzr1/Mfr1 and Slp1 in fission yeast. Mol Biol Cell. 2011;22:1486-94 pubmed publisher
    ..We study all the coactivators present in the yeast genome and find that only Slp1/Cdc20 is essential for meiosis I progression...
  8. Elmore Z, Beckley J, Chen J, Gould K. Histone H2B ubiquitination promotes the function of the anaphase-promoting complex/cyclosome in Schizosaccharomyces pombe. G3 (Bethesda). 2014;4:1529-38 pubmed publisher
    ..associated DUB, suppressed temperature-sensitive phenotypes of APC/C mutants cut9-665, lid1-6, cut4-533, and slp1-362...
  9. Zich J, May K, Paraskevopoulos K, Sen O, Syred H, van der Sar S, et al. Mps1Mph1 Kinase Phosphorylates Mad3 to Inhibit Cdc20Slp1-APC/C and Maintain Spindle Checkpoint Arrests. PLoS Genet. 2016;12:e1005834 pubmed publisher
    ..phospho-mimics are potent APC/C inhibitors in vitro, demonstrating that Mad3p modification can directly influence Cdc20(Slp1)-APC/C activity...