rpt2

Summary

Gene Symbol: rpt2
Description: 19S proteasome regulatory subunit Rpt2
Alias: mts2, 19S proteasome regulatory subunit Rpt2
Species: fission yeast

Top Publications

  1. Gordon C, McGurk G, Dillon P, Rosen C, Hastie N. Defective mitosis due to a mutation in the gene for a fission yeast 26S protease subunit. Nature. 1993;366:355-7 pubmed
    We have isolated a mutant, mts2, in the fission yeast Schizosaccharomyces pombe which is defective in chromosome segregation...
  2. Yen H, Gordon C, Chang E. Schizosaccharomyces pombe Int6 and Ras homologs regulate cell division and mitotic fidelity via the proteasome. Cell. 2003;112:207-17 pubmed
    ..We propose that human Int6, either alone or cooperatively with Ras, influences proteasome activities via Rpn5. Inactivating Int6 can lead to accumulation of mitotic regulators affecting cell division and mitotic fidelity. ..
  3. Matsuo Y, Kishimoto H, Tanae K, Kitamura K, Katayama S, Kawamukai M. Nuclear protein quality is regulated by the ubiquitin-proteasome system through the activity of Ubc4 and San1 in fission yeast. J Biol Chem. 2011;286:13775-90 pubmed publisher
    ..The Asf1-30 mutant protein was highly ubiquitinated at higher temperatures, and it remained stable in an mts2-1 mutant, which lacks proteasome activity...
  4. Penney M, Samejima I, Wilkinson C, McInerny C, Mathiassen S, Wallace M, et al. Fission yeast 26S proteasome mutants are multi-drug resistant due to stabilization of the Pap1 transcription factor. PLoS ONE. 2012;7:e50796 pubmed publisher
    ..Accordingly, mutants lacking Rhp6 or Ubr1 display drug-resistant phenotypes. ..
  5. Wilkinson C, Wallace M, Seeger M, Dubiel W, Gordon C. Mts4, a non-ATPase subunit of the 26 S protease in fission yeast is essential for mitosis and interacts directly with the ATPase subunit Mts2. J Biol Chem. 1997;272:25768-77 pubmed
    ..We provide genetic and physical evidence to suggest that the Mts4 protein interacts with the product of the mts2(+) gene, an ATPase which has previously been shown to be subunit 4 of the 26 S protease.
  6. Yen H, Espiritu C, Chang E. Rpn5 is a conserved proteasome subunit and required for proper proteasome localization and assembly. J Biol Chem. 2003;278:30669-76 pubmed
    ..Human Rpn5 rescues the phenotypes associated with rpn5Delta and is incorporated into the yeast proteasome, suggesting that Rpn5 functions are highly conserved. ..
  7. Dunand Sauthier I, Walker C, Wilkinson C, Gordon C, Crane R, Norbury C, et al. Sum1, a component of the fission yeast eIF3 translation initiation complex, is rapidly relocalized during environmental stress and interacts with components of the 26S proteasome. Mol Biol Cell. 2002;13:1626-40 pubmed
    ..Furthermore, Sum1 interacts with the Mts2 and Mts4 components of the 26S proteasome...
  8. Stone M, Hartmann Petersen R, Seeger M, Bech Otschir D, Wallace M, Gordon C. Uch2/Uch37 is the major deubiquitinating enzyme associated with the 26S proteasome in fission yeast. J Mol Biol. 2004;344:697-706 pubmed
    ..of Ubp6 appears to play a more regulatory and/or structural role involving the proteasome subunits Mts1/Rpn9, Mts2/Rpt2 and Mts3/Rpn12, as Ubp6 becomes essential when activity of these subunits is compromised by conditional mutations...
  9. Selvanathan S, Thakurta A, Dhakshnamoorthy J, Zhou M, Veenstra T, Dhar R. Schizosaccharomyces pombe Dss1p is a DNA damage checkpoint protein that recruits Rad24p, Cdc25p, and Rae1p to DNA double-strand breaks. J Biol Chem. 2010;285:14122-33 pubmed publisher
    ..We suggest that the sequestration of Cdc25p to DNA damage sites could provide a mechanism for S. pombe cells to arrest at G(2)/M boundary in response to DNA damage. ..

More Information

Publications15

  1. Kriegenburg F, Jakopec V, Poulsen E, Nielsen S, Roguev A, Krogan N, et al. A chaperone-assisted degradation pathway targets kinetochore proteins to ensure genome stability. PLoS Genet. 2014;10:e1004140 pubmed publisher
  2. Seeger M, Hartmann Petersen R, Wilkinson C, Wallace M, Samejima I, Taylor M, et al. Interaction of the anaphase-promoting complex/cyclosome and proteasome protein complexes with multiubiquitin chain-binding proteins. J Biol Chem. 2003;278:16791-6 pubmed
    ..The putative role of Pus1 as a factor involved in allocation of ubiquitinylated substrates for the proteasome is discussed. ..
  3. Jossé L, Harley M, Pires I, Hughes D. Fission yeast Dss1 associates with the proteasome and is required for efficient ubiquitin-dependent proteolysis. Biochem J. 2006;393:303-9 pubmed
    ..These findings show that DSS1 has an evolutionarily conserved role as a regulator of proteasome function and suggest that DSS1 may provide a link between BRCA2 and ubiquitin-mediated proteolysis in human cells. ..
  4. Cabrera R, Sha Z, Vadakkan T, Otero J, Kriegenburg F, Hartmann Petersen R, et al. Proteasome nuclear import mediated by Arc3 can influence efficient DNA damage repair and mitosis in Schizosaccharomyces pombe. Mol Biol Cell. 2010;21:3125-36 pubmed publisher
    ..These data suggest that proteasomes display Arc3-dependent mobility in the cell, and mobile proteasomes can efficiently access substrates throughout the cell, allowing them to effectively regulate cell-compartment-specific activities. ..
  5. Kitagawa T, Ishii K, Takeda K, Matsumoto T. The 19S proteasome subunit Rpt3 regulates distribution of CENP-A by associating with centromeric chromatin. Nat Commun. 2014;5:3597 pubmed publisher
    ..Thus, we propose that the proteasome (or its subcomplex) associates with centromeric chromatin and regulates distribution of CENP-A...
  6. Kampmeyer C, Karakostova A, Schenstrøm S, Abildgaard A, Lauridsen A, Jourdain I, et al. The exocyst subunit Sec3 is regulated by a protein quality control pathway. J Biol Chem. 2017;292:15240-15253 pubmed publisher
    ..One of the suppressing DNAs encoded a truncated dominant-negative variant of the 26S proteasome subunit, Rpt2, indicating that exocyst degradation is controlled by the ubiquitin-proteasome system...