Gene Symbol: rpn12
Description: 19S proteasome regulatory subunit Rpn12
Alias: SPBC342.07, mts3, 19S proteasome regulatory subunit Rpn12
Species: fission yeast

Top Publications

  1. Penney M, Wilkinson C, Wallace M, Javerzat J, Ferrell K, Seeger M, et al. The Pad1+ gene encodes a subunit of the 26 S proteasome in fission yeast. J Biol Chem. 1998;273:23938-45 pubmed
    ..This screen has previously isolated mutations in the 26 S proteasome subunits Mts2, Mts3, and Mts4. We show that the mutation in the mts5-1 strain occurs in the pad1(+) gene...
  2. Wilkinson C, Ferrell K, Penney M, Wallace M, Dubiel W, Gordon C. Analysis of a gene encoding Rpn10 of the fission yeast proteasome reveals that the polyubiquitin-binding site of this subunit is essential when Rpn12/Mts3 activity is compromised. J Biol Chem. 2000;275:15182-92 pubmed
    ..is synthetically lethal with mutations in other proteasomal component-encoding genes, namely mts3, pad1, and mts4 (RPN12, RPN11, and RPN1)...
  3. Gordon C, McGurk G, Wallace M, Hastie N. A conditional lethal mutant in the fission yeast 26 S protease subunit mts3+ is defective in metaphase to anaphase transition. J Biol Chem. 1996;271:5704-11 pubmed
    We have isolated a conditional lethal mutant mts3 in the fission yeast Schizosaccharomyces pombe which at the permissive temperature is resistant to the mitotic poison MBC and at the restrictive temperature is defective in metaphase to ..
  4. Riedinger C, Boehringer J, Trempe J, Lowe E, Brown N, Gehring K, et al. Structure of Rpn10 and its interactions with polyubiquitin chains and the proteasome subunit Rpn12. J Biol Chem. 2010;285:33992-4003 pubmed publisher
    ..This is the first observation of a UIM binding other than a Ub fold and suggests that SpRpn12 could modulate the activity of SpRpn10 as a proteasomal Ub receptor. ..
  5. Ikai N, Yanagida M. Cdc48 is required for the stability of Cut1/separase in mitotic anaphase. J Struct Biol. 2006;156:50-61 pubmed
    ..Cells lose viability during the traverse of anaphase in cdc48 mutant cells. Cdc48 may protect Cut1/separase and Cut2/securin against the instability during polyubiquitination and degradation in the metaphase-anaphase transition. ..
  6. Kitagawa T, Ishii K, Takeda K, Matsumoto T. The 19S proteasome subunit Rpt3 regulates distribution of CENP-A by associating with centromeric chromatin. Nat Commun. 2014;5:3597 pubmed publisher
    ..Thus, we propose that the proteasome (or its subcomplex) associates with centromeric chromatin and regulates distribution of CENP-A...
  7. Jossé L, Harley M, Pires I, Hughes D. Fission yeast Dss1 associates with the proteasome and is required for efficient ubiquitin-dependent proteolysis. Biochem J. 2006;393:303-9 pubmed
    ..First, overexpression of two components of the 19 S RP, namely Pad1/Rpn11 and Mts3/Rpn12, rescued the temperature-sensitive growth defect of the dss1 mutant...
  8. Takeda K, Yanagida M. Regulation of nuclear proteasome by Rhp6/Ubc2 through ubiquitination and destruction of the sensor and anchor Cut8. Cell. 2005;122:393-405 pubmed
    ..The short-lived nature of Cut8 is crucial for feedback enrichment of the proteasome within the nucleus. This is likely to be a conserved mechanism as we describe a Cut8 homolog in flies. ..
  9. Harrison C, Katayama S, Dhut S, Chen D, Jones N, Bahler J, et al. SCF(Pof1)-ubiquitin and its target Zip1 transcription factor mediate cadmium response in fission yeast. EMBO J. 2005;24:599-610 pubmed
    ..Our results indicate that Zip1 mediates growth arrest in cadmium response, which is essential to maintain viability. Normally growing cells prevent this response through constitutive ubiquitylation and degradation of Zip1 via SCF(Pof1). ..

More Information


  1. Yen H, Gordon C, Chang E. Schizosaccharomyces pombe Int6 and Ras homologs regulate cell division and mitotic fidelity via the proteasome. Cell. 2003;112:207-17 pubmed
    ..We propose that human Int6, either alone or cooperatively with Ras, influences proteasome activities via Rpn5. Inactivating Int6 can lead to accumulation of mitotic regulators affecting cell division and mitotic fidelity. ..
  2. Tatebe H, Yanagida M. Cut8, essential for anaphase, controls localization of 26S proteasome, facilitating destruction of cyclin and Cut2. Curr Biol. 2000;10:1329-38 pubmed
    ..Cut8 is a novel evolutionarily conserved heat-inducible regulator. It facilitates anaphase-promoting proteolysis by recruiting 26S proteasome to a functionally efficient nuclear location. ..
  3. Yen H, Espiritu C, Chang E. Rpn5 is a conserved proteasome subunit and required for proper proteasome localization and assembly. J Biol Chem. 2003;278:30669-76 pubmed
    ..Human Rpn5 rescues the phenotypes associated with rpn5Delta and is incorporated into the yeast proteasome, suggesting that Rpn5 functions are highly conserved. ..
  4. Boehringer J, Riedinger C, Paraskevopoulos K, Johnson E, Lowe E, Khoudian C, et al. Structural and functional characterization of Rpn12 identifies residues required for Rpn10 proteasome incorporation. Biochem J. 2012;448:55-65 pubmed publisher
    The ubiquitin-proteasome system targets selected proteins for degradation by the 26S proteasome. Rpn12 is an essential component of the 19S regulatory particle and plays a role in recruiting the extrinsic ubiquitin receptor Rpn10...
  5. Penney M, Samejima I, Wilkinson C, McInerny C, Mathiassen S, Wallace M, et al. Fission yeast 26S proteasome mutants are multi-drug resistant due to stabilization of the Pap1 transcription factor. PLoS ONE. 2012;7:e50796 pubmed publisher
    ..Accordingly, mutants lacking Rhp6 or Ubr1 display drug-resistant phenotypes. ..
  6. Stone M, Hartmann Petersen R, Seeger M, Bech Otschir D, Wallace M, Gordon C. Uch2/Uch37 is the major deubiquitinating enzyme associated with the 26S proteasome in fission yeast. J Mol Biol. 2004;344:697-706 pubmed
    ..to play a more regulatory and/or structural role involving the proteasome subunits Mts1/Rpn9, Mts2/Rpt2 and Mts3/Rpn12, as Ubp6 becomes essential when activity of these subunits is compromised by conditional mutations...
  7. Sha Z, Yen H, Scheel H, Suo J, Hofmann K, Chang E. Isolation of the Schizosaccharomyces pombe proteasome subunit Rpn7 and a structure-function study of the proteasome-COP9-initiation factor domain. J Biol Chem. 2007;282:32414-23 pubmed
    ..Disruption of such an arrangement in the PCI domain may substantially inactivate many PCI proteins and block their binding to other proteins. ..
  8. Takeda K, Yoshida T, Kikuchi S, Nagao K, Kokubu A, Pluskal T, et al. Synergistic roles of the proteasome and autophagy for mitochondrial maintenance and chronological lifespan in fission yeast. Proc Natl Acad Sci U S A. 2010;107:3540-5 pubmed publisher
    ..Proteasome and autophagy are thus collaborative to support the lifespan of S. pombe G0 phase. ..