Gene Symbol: rpn11
Description: 19S proteasome regulatory subunit Rpn11
Alias: bfr2, mts5, pad1, sks1, 19S proteasome regulatory subunit Rpn11
Species: fission yeast

Top Publications

  1. Andersen K, Jensen C, Kriegenburg F, Lauridsen A, Gordon C, Hartmann Petersen R. Txl1 and Txc1 are co-factors of the 26S proteasome in fission yeast. Antioxid Redox Signal. 2011;14:1601-8 pubmed publisher
    ..Accordingly, Txl1 was able to reduce and mediate the degradation of an oxidized model proteasome substrate in vitro. Thus, Txl1 and Txc1 are proteasome co-factors connected with oxidative stress. ..
  2. Stone M, Hartmann Petersen R, Seeger M, Bech Otschir D, Wallace M, Gordon C. Uch2/Uch37 is the major deubiquitinating enzyme associated with the 26S proteasome in fission yeast. J Mol Biol. 2004;344:697-706 pubmed
  3. Penney M, Wilkinson C, Wallace M, Javerzat J, Ferrell K, Seeger M, et al. The Pad1+ gene encodes a subunit of the 26 S proteasome in fission yeast. J Biol Chem. 1998;273:23938-45 pubmed
    We have isolated a fission yeast mutant, mts5-1, in a screen for mutations that confer both methyl 2-benzimidazolecarbamate resistance (MBCR) and temperature sensitivity (ts) on Schizosaccharomyces pombe...
  4. Wilkinson C, Wallace M, Morphew M, Perry P, Allshire R, Javerzat J, et al. Localization of the 26S proteasome during mitosis and meiosis in fission yeast. EMBO J. 1998;17:6465-76 pubmed
    ..Furthermore they raise interesting questions as to the spatial organization of protein degradation during meiosis. ..
  5. Arioka M, Kouhashi M, Yoda K, Takatsuki A, Yamasaki M, Kitamoto K. Multidrug resistance phenotype conferred by overexpressing bfr2+/pad1+/sks1+ or pap1+ genes and mediated by bfr1+ gene product, a structural and functional homologue of P-glycoprotein in Schizosaccharomyces pombe. Biosci Biotechnol Biochem. 1998;62:390-2 pubmed
    We investigated the mechanism of multidrug resistance conferred by overexpression of bfr2+/pad1+/sks1+ or pap1+ genes of Schizosaccharomyces pombe. Overexpression of bfr2+ did not confer multidrug resistance on a pap1-disrupted strain...
  6. Penney M, Samejima I, Wilkinson C, McInerny C, Mathiassen S, Wallace M, et al. Fission yeast 26S proteasome mutants are multi-drug resistant due to stabilization of the Pap1 transcription factor. PLoS ONE. 2012;7:e50796 pubmed publisher
    ..Accordingly, mutants lacking Rhp6 or Ubr1 display drug-resistant phenotypes. ..
  7. Elmore Z, Beckley J, Chen J, Gould K. Histone H2B ubiquitination promotes the function of the anaphase-promoting complex/cyclosome in Schizosaccharomyces pombe. G3 (Bethesda). 2014;4:1529-38 pubmed publisher
    ..On the basis of these data, we conclude that Ubp8 antagonizes APC/C function indirectly by modulating H2B ubiquitination status. ..
  8. Takeda K, Yoshida T, Kikuchi S, Nagao K, Kokubu A, Pluskal T, et al. Synergistic roles of the proteasome and autophagy for mitochondrial maintenance and chronological lifespan in fission yeast. Proc Natl Acad Sci U S A. 2010;107:3540-5 pubmed publisher
    ..Proteasome and autophagy are thus collaborative to support the lifespan of S. pombe G0 phase. ..
  9. Kriegenburg F, Jakopec V, Poulsen E, Nielsen S, Roguev A, Krogan N, et al. A chaperone-assisted degradation pathway targets kinetochore proteins to ensure genome stability. PLoS Genet. 2014;10:e1004140 pubmed publisher

More Information


  1. Takeda K, Yanagida M. Regulation of nuclear proteasome by Rhp6/Ubc2 through ubiquitination and destruction of the sensor and anchor Cut8. Cell. 2005;122:393-405 pubmed
    ..The short-lived nature of Cut8 is crucial for feedback enrichment of the proteasome within the nucleus. This is likely to be a conserved mechanism as we describe a Cut8 homolog in flies. ..
  2. Usui T, Yoshida M, Honda A, Beppu T, Horinouchi S. A K-252a-resistance gene, sks1+, encodes a protein similar to the Caenorhabditis elegans F37 A4.5 gene product and confers multidrug resistance in Schizosaccharomyces pombe. Gene. 1995;161:93-6 pubmed
    A gene named sks1+ was cloned as a suppressor of the K-252a-sensitivity phenotype of Schizosaccharomyces pombe (Sp) from a gene library of the parental Sp chromosomal DNA constructed with a multicopy vector pDB248'...
  3. Kumada K, Yanagida M, Toda T. Caffeine-resistance in fission yeast is caused by mutations in a single essential gene, crm1+. Mol Gen Genet. 1996;250:59-68 pubmed
    ..These results show that the crm1+ gene is a major locus for caffeine resistance, which arises from Pap1-dependent transcriptional activation. ..
  4. Jossé L, Harley M, Pires I, Hughes D. Fission yeast Dss1 associates with the proteasome and is required for efficient ubiquitin-dependent proteolysis. Biochem J. 2006;393:303-9 pubmed
    ..First, overexpression of two components of the 19 S RP, namely Pad1/Rpn11 and Mts3/Rpn12, rescued the temperature-sensitive growth defect of the dss1 mutant...
  5. Yen H, Espiritu C, Chang E. Rpn5 is a conserved proteasome subunit and required for proper proteasome localization and assembly. J Biol Chem. 2003;278:30669-76 pubmed
    ..Human Rpn5 rescues the phenotypes associated with rpn5Delta and is incorporated into the yeast proteasome, suggesting that Rpn5 functions are highly conserved. ..
  6. Saito Y, Takeda J, Okada M, Kobayashi J, Kato A, Hirota K, et al. The proteasome factor Bag101 binds to Rad22 and suppresses homologous recombination. Sci Rep. 2013;3:2022 pubmed publisher
    ..Rad22 physically interacted with the BAG domain of Bag101, and a lack of this domain enhanced HR frequency. Similarly, radiation exposure triggered the dissociation of these proteins so that Rad22 was stabilized and able to enhance HR...
  7. Wilkinson C, Ferrell K, Penney M, Wallace M, Dubiel W, Gordon C. Analysis of a gene encoding Rpn10 of the fission yeast proteasome reveals that the polyubiquitin-binding site of this subunit is essential when Rpn12/Mts3 activity is compromised. J Biol Chem. 2000;275:15182-92 pubmed
    ..lethal with mutations in other proteasomal component-encoding genes, namely mts3, pad1, and mts4 (RPN12, RPN11, and RPN1)...
  8. Mannen T, Andoh T, Tani T. Dss1 associating with the proteasome functions in selective nuclear mRNA export in yeast. Biochem Biophys Res Commun. 2008;365:664-71 pubmed
    ..We identified the pad1(+) gene, which encodes a component of the 26S proteasome, as a suppressor for the ts(-) phenotype of Deltadss1...
  9. Ambroggio X, Rees D, Deshaies R. JAMM: a metalloprotease-like zinc site in the proteasome and signalosome. PLoS Biol. 2004;2:E2 pubmed publisher
    The JAMM (JAB1/MPN/Mov34 metalloenzyme) motif in Rpn11 and Csn5 underlies isopeptidase activities intrinsic to the proteasome and signalosome, respectively...