Gene Symbol: rpn10
Description: 19S proteasome regulatory subunit Rpn10
Alias: pus1, 19S proteasome regulatory subunit Rpn10
Species: fission yeast

Top Publications

  1. Wilkinson C, Ferrell K, Penney M, Wallace M, Dubiel W, Gordon C. Analysis of a gene encoding Rpn10 of the fission yeast proteasome reveals that the polyubiquitin-binding site of this subunit is essential when Rpn12/Mts3 activity is compromised. J Biol Chem. 2000;275:15182-92 pubmed
    ..Deletion of the corresponding Saccharomyces cerevisiae gene, MCB1/RPN10, rendered cells viable indicating that other proteasomal polyubiquitin receptors must exist...
  2. Wilkinson C, Seeger M, Hartmann Petersen R, Stone M, Wallace M, Semple C, et al. Proteins containing the UBA domain are able to bind to multi-ubiquitin chains. Nat Cell Biol. 2001;3:939-43 pubmed
    ..These two proteins are implicated, along with the fission-yeast Pus1(S5a/Rpn10) subunit of the 26 S proteasome, in the recognition and turnover of substrates by this proteolytic complex.
  3. Seeger M, Hartmann Petersen R, Wilkinson C, Wallace M, Samejima I, Taylor M, et al. Interaction of the anaphase-promoting complex/cyclosome and proteasome protein complexes with multiubiquitin chain-binding proteins. J Biol Chem. 2003;278:16791-6 pubmed
    Fission yeast Rhp23 and Pus1 represent two families of multiubiquitin chain-binding proteins that associate with the proteasome. We show that both proteins bind to different regions of the proteasome subunit Mts4...
  4. Jossé L, Harley M, Pires I, Hughes D. Fission yeast Dss1 associates with the proteasome and is required for efficient ubiquitin-dependent proteolysis. Biochem J. 2006;393:303-9 pubmed
    ..These findings show that DSS1 has an evolutionarily conserved role as a regulator of proteasome function and suggest that DSS1 may provide a link between BRCA2 and ubiquitin-mediated proteolysis in human cells. ..
  5. Riedinger C, Boehringer J, Trempe J, Lowe E, Brown N, Gehring K, et al. Structure of Rpn10 and its interactions with polyubiquitin chains and the proteasome subunit Rpn12. J Biol Chem. 2010;285:33992-4003 pubmed publisher
    Schizosaccharomyces pombe Rpn10 (SpRpn10) is a proteasomal ubiquitin (Ub) receptor located within the 19 S regulatory particle where it binds to subunits of both the base and lid subparticles...
  6. Hartmann Petersen R, Hendil K, Gordon C. Ubiquitin binding proteins protect ubiquitin conjugates from disassembly. FEBS Lett. 2003;535:77-81 pubmed
    ..Here we report that three ubiquitin binding proteins, Rhp23, Dph1 and Pus1, from fission yeast can protect multiubiquitin conjugates against deubiquitination...
  7. Yen H, Gordon C, Chang E. Schizosaccharomyces pombe Int6 and Ras homologs regulate cell division and mitotic fidelity via the proteasome. Cell. 2003;112:207-17 pubmed
    ..We propose that human Int6, either alone or cooperatively with Ras, influences proteasome activities via Rpn5. Inactivating Int6 can lead to accumulation of mitotic regulators affecting cell division and mitotic fidelity. ..
  8. Yen H, Espiritu C, Chang E. Rpn5 is a conserved proteasome subunit and required for proper proteasome localization and assembly. J Biol Chem. 2003;278:30669-76 pubmed
    ..Human Rpn5 rescues the phenotypes associated with rpn5Delta and is incorporated into the yeast proteasome, suggesting that Rpn5 functions are highly conserved. ..
  9. Stone M, Hartmann Petersen R, Seeger M, Bech Otschir D, Wallace M, Gordon C. Uch2/Uch37 is the major deubiquitinating enzyme associated with the 26S proteasome in fission yeast. J Mol Biol. 2004;344:697-706 pubmed

More Information


  1. Sha Z, Yen H, Scheel H, Suo J, Hofmann K, Chang E. Isolation of the Schizosaccharomyces pombe proteasome subunit Rpn7 and a structure-function study of the proteasome-COP9-initiation factor domain. J Biol Chem. 2007;282:32414-23 pubmed
    ..Disruption of such an arrangement in the PCI domain may substantially inactivate many PCI proteins and block their binding to other proteins. ..
  2. Paraskevopoulos K, Kriegenburg F, Tatham M, Rösner H, Medina B, Larsen I, et al. Dss1 is a 26S proteasome ubiquitin receptor. Mol Cell. 2014;56:453-61 pubmed publisher
    ..The proteasome subunits Rpn10 and Rpn13 are known to bind ubiquitin, but genetic and biochemical data suggest the existence of at least one ..
  3. Hartmann Petersen R, Wallace M, Hofmann K, Koch G, Johnsen A, Hendil K, et al. The Ubx2 and Ubx3 cofactors direct Cdc48 activity to proteolytic and nonproteolytic ubiquitin-dependent processes. Curr Biol. 2004;14:824-8 pubmed
    ..Our genetic analyses revealed that Ubx3/p47 functionally parallels a substrate receptor of the 26S proteasome, Pus1/Rpn10, indicating that the Cdc48-Ubx3 complex is involved in delivering substrates to the 26S proteasome.
  4. Boehringer J, Riedinger C, Paraskevopoulos K, Johnson E, Lowe E, Khoudian C, et al. Structural and functional characterization of Rpn12 identifies residues required for Rpn10 proteasome incorporation. Biochem J. 2012;448:55-65 pubmed publisher
    ..essential component of the 19S regulatory particle and plays a role in recruiting the extrinsic ubiquitin receptor Rpn10. In the present paper we report the crystal structure of Rpn12, a proteasomal PCI-domain-containing protein...