rpb7

Summary

Gene Symbol: rpb7
Description: DNA-directed RNA polymerase complex II subunit Rpb7
Alias: SPAPYUK71.02, DNA-directed RNA polymerase complex II subunit Rpb7
Species: fission yeast

Top Publications

  1. Djupedal I, Portoso M, Spahr H, Bonilla C, Gustafsson C, Allshire R, et al. RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing. Genes Dev. 2005;19:2301-6 pubmed
    ..Here we show that another Pol II subunit, Rpb7 has a specific role in pre-siRNA transcription...
  2. Armache K, Mitterweger S, Meinhart A, Cramer P. Structures of complete RNA polymerase II and its subcomplex, Rpb4/7. J Biol Chem. 2005;280:7131-4 pubmed
    ..formation of an alpha-helix in the linker region of the largest Pol II subunit and with folding of the conserved Rpb7 tip loop...
  3. Montes M, Moreira Ramos S, Rojas D, Urbina F, Käufer N, Maldonado E. RNA polymerase II components and Rrn7 form a preinitiation complex on the HomolD box to promote ribosomal protein gene expression in Schizosaccharomyces pombe. FEBS J. 2017;284:615-633 pubmed publisher
    ..Together, these data show that components of the RNAPII machinery and Rrn7 participate in the PIC assembly on the HomolD box, thereby directing RPG transcription. ..
  4. Suzuki S, Kato H, Suzuki Y, Chikashige Y, Hiraoka Y, Kimura H, et al. Histone H3K36 trimethylation is essential for multiple silencing mechanisms in fission yeast. Nucleic Acids Res. 2016;44:4147-62 pubmed publisher
  5. Suzuki S, Nagao K, Obuse C, Murakami Y, Takahata S. A novel method for purification of the endogenously expressed fission yeast Set2 complex. Protein Expr Purif. 2014;97:44-9 pubmed publisher
    ..In conclusion, the FIM method is valid for the efficient purification of protein complexes that separate into the insoluble chromatin pellet fraction during centrifugation. ..
  6. Sakurai H, Ishihama A. Gene organization and protein sequence of the small subunits of Schizosaccharomyces pombe RNA polymerase II. Gene. 1997;196:165-74 pubmed
    ..Here, we cloned both cDNA and the genes encoding four small subunits, Rpb7, Rpb8, Rpb10 and Rpb11...
  7. Kimura M, Suzuki H, Ishihama A. Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II. Mol Cell Biol. 2002;22:1577-88 pubmed
    ..This result indicates the importance of Fcp1-Rpb4 interaction for formation of the Fcp1/TFIIF/pol II complex in vivo. ..
  8. Kimura M, Ishihama A. Involvement of multiple subunit-subunit contacts in the assembly of RNA polymerase II. Nucleic Acids Res. 2000;28:952-9 pubmed
    ..on glutathione and Ni(2+)columns, complexes consisting of the seven subunits, Rpb1H, Rpb2, GST-Rpb3, Rpb5, Rpb7, Rpb8 and Rpb11, were identified...
  9. Mitsuzawa H, Kimura M, Kanda E, Ishihama A. Glyceraldehyde-3-phosphate dehydrogenase and actin associate with RNA polymerase II and interact with its Rpb7 subunit. FEBS Lett. 2005;579:48-52 pubmed
    ..Here, we provide evidence that the Rpb7 subunit of pol II interacts with GAPDH. Two-hybrid screen identified GAPDH as an Rpb7-binding protein...

More Information

Publications21

  1. Spahr H, Beve J, Larsson T, Bergstrom J, Karlsson K, Gustafsson C. Purification and characterization of RNA polymerase II holoenzyme from Schizosaccharomyces pombe. J Biol Chem. 2000;275:1351-6 pubmed
    ..cerevisiae. We suggest that the overall structure and mechanism of the Mediator is evolutionary conserved. The subunit composition, however, has evolved to respond properly to physiological signals. ..
  2. Mitsuzawa H, Kanda E, Ishihama A. Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts. Nucleic Acids Res. 2003;31:4696-701 pubmed
    Rpb4-Rpb7, a dissociable subcomplex of RNA polymerase II (pol II), is required for transcription initiation...
  3. Hayashi K, Watanabe T, Tanaka A, Furumoto T, Sato Tsuchiya C, Kimura M, et al. Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functional changes in RNA polymerase II at transcription initiation. Genes Cells. 2005;10:207-24 pubmed
  4. Bah A, Wischnewski H, Shchepachev V, Azzalin C. The telomeric transcriptome of Schizosaccharomyces pombe. Nucleic Acids Res. 2012;40:2995-3005 pubmed publisher
    ..We also show that the RNAPII subunit Rpb7 and the non-canonical poly(A) polymerases Cid12 and Cid14 are involved in the regulation of TERRA, ARIA, ARRET and ?..
  5. Ishiguro A, Kimura M, Yasui K, Iwata A, Ueda S, Ishihama A. Two large subunits of the fission yeast RNA polymerase II provide platforms for the assembly of small subunits. J Mol Biol. 1998;279:703-12 pubmed
    ..of 19 combinations, including five combinations between small subunits, Rpb3-Rpb10, Rpb3-Rpb11, Rpb5-Rpb6, Rpb6-Rpb7 and Rpb6-Rpb8...
  6. Tucker J, Ohle C, Schermann G, Bendrin K, Zhang W, Fischer T, et al. A Novel Epigenetic Silencing Pathway Involving the Highly Conserved 5'-3' Exoribonuclease Dhp1/Rat1/Xrn2 in Schizosaccharomyces pombe. PLoS Genet. 2016;12:e1005873 pubmed publisher
    ..The results describe the unexpected role of Dhp1/Rat1/Xrn2 in chromatin-based silencing and elucidate how various RNA-processing pathways, acting together or independently, contribute to epigenetic regulation of the eukaryotic genome. ..
  7. Kimura M, Ishiguro A, Ishihama A. RNA polymerase II subunits 2, 3, and 11 form a core subassembly with DNA binding activity. J Biol Chem. 1997;272:25851-5 pubmed
    ..Both the Rpb2-Rpb3-Rpb11 complex and the free Rpb1 protein showed DNA binding activity, although the affinity was weaker compared with the intact RNA polymerase. ..
  8. Kimura M, Sakurai H, Ishihama A. Intracellular contents and assembly states of all 12 subunits of the RNA polymerase II in the fission yeast Schizosaccharomyces pombe. Eur J Biochem. 2001;268:612-9 pubmed
    ..In agreement with the biochemical analysis, the GFP-Rpb1 and GFP-Rpb3 fusions were present in the nuclei but the GFP-Rpb4 was detected in the cytoplasm as well as the nuclei. ..
  9. Tamayo E, Bernal G, Teno U, Maldonado E. Mediator is required for activated transcription in a Schizosaccharomyces pombe in vitro system. Eur J Biochem. 2004;271:2561-72 pubmed
    ..pombe system. These results indicate that Sc. pombe uses the same set of general transcription factors as other eukaryotes and that the Mediator is involved in activated transcription. ..
  10. Spahr H, Calero G, Bushnell D, Kornberg R. Schizosacharomyces pombe RNA polymerase II at 3.6-A resolution. Proc Natl Acad Sci U S A. 2009;106:9185-90 pubmed publisher
    ..pombe and humans. Amino acid changes apparently responsible for the structural differences are also conserved between S. pombe and humans, suggesting that the S. pombe structure may be a good surrogate for that of the human enzyme. ..
  11. Sakurai H, Mitsuzawa H, Kimura M, Ishihama A. The Rpb4 subunit of fission yeast Schizosaccharomyces pombe RNA polymerase II is essential for cell viability and similar in structure to the corresponding subunits of higher eukaryotes. Mol Cell Biol. 1999;19:7511-8 pubmed
    ..pombe Rpb4 formed stable hybrid heterodimers with S. cerevisiae RPB7, suggesting that S...
  12. Kumar D, Sharma N. Modulating the level of the Rpb7 subunit of RNA polymerase II affects cell separation in Schizosaccharomyces pombe. Res Microbiol. 2015;166:20-7 pubmed publisher
    The rpb7(+) gene encodes the seventh largest subunit of RNA polymerase II and is essential for survival of yeast cells...