rng2

Summary

Gene Symbol: rng2
Description: IQ motif containing GTPase activating protein
Alias: IQ motif containing GTPase activating protein
Species: fission yeast

Top Publications

  1. Wu J, Bahler J, Pringle J. Roles of a fimbrin and an alpha-actinin-like protein in fission yeast cell polarization and cytokinesis. Mol Biol Cell. 2001;12:1061-77 pubmed
    ..In addition, protein-localization and mutant-phenotype data suggest that Fim1p, but not Ain1p, plays important roles in mating and in spore formation. ..
  2. Laporte D, Coffman V, Lee I, Wu J. Assembly and architecture of precursor nodes during fission yeast cytokinesis. J Cell Biol. 2011;192:1005-21 pubmed publisher
    ..Mid1 first recruits Cdc4 and IQGAP Rng2 to form module I. Rng2 subsequently recruits the myosin-II subunits Myo2 and Rlc1...
  3. Chang F, Woollard A, Nurse P. Isolation and characterization of fission yeast mutants defective in the assembly and placement of the contractile actin ring. J Cell Sci. 1996;109 ( Pt 1):131-42 pubmed
    ..cdc4 and rng2 mutants display disorganized actin cables during mitosis, but normal interphase actin patterns...
  4. Mishra M, Karagiannis J, Trautmann S, Wang H, McCollum D, Balasubramanian M. The Clp1p/Flp1p phosphatase ensures completion of cytokinesis in response to minor perturbation of the cell division machinery in Schizosaccharomyces pombe. J Cell Sci. 2004;117:3897-910 pubmed
    ..We conclude that the Clp1p/Flp1p-dependent cytokinesis checkpoint provides a previously unrecognized cell survival advantage when the cell division apparatus is mildly perturbed. ..
  5. Wang C, Balasubramanian M, Dokland T. Structure, crystal packing and molecular dynamics of the calponin-homology domain of Schizosaccharomyces pombe Rng2. Acta Crystallogr D Biol Crystallogr. 2004;60:1396-403 pubmed
    Schizosaccharomyces pombe Rng2 is an IQGAP protein that is essential for the assembly of an actomyosin ring during cytokinesis. Rng2 contains an amino-terminal calponin-homology (CH) domain, 11 IQ repeats and a RasGAP-homology domain...
  6. Padmanabhan A, Bakka K, Sevugan M, Naqvi N, D souza V, Tang X, et al. IQGAP-related Rng2p organizes cortical nodes and ensures position of cell division in fission yeast. Curr Biol. 2011;21:467-72 pubmed publisher
    ..We conclude that Mid1p recruits Rng2p to cortical nodes at the division site and that Rng2p, in turn, recruits other components of the actomyosin ring to cortical nodes, thereby ensuring correct placement of the division site. ..
  7. Takaine M, Numata O, Nakano K. Fission yeast IQGAP arranges actin filaments into the cytokinetic contractile ring. EMBO J. 2009;28:3117-31 pubmed publisher
    ..We show that the fission yeast Schizosaccharomyces pombe IQGAP Rng2 is involved in the generation of CR F-actin and required for its arrangement into a ring...
  8. Eng K, Naqvi N, Wong K, Balasubramanian M. Rng2p, a protein required for cytokinesis in fission yeast, is a component of the actomyosin ring and the spindle pole body. Curr Biol. 1998;8:611-21 pubmed
    ..Here, we have characterized one such mutant, rng2. Temperature-sensitive rng2 mutants accumulated F-actin cables in the medial region of the cell but failed to ..
  9. Lee I, Wu J. Characterization of Mid1 domains for targeting and scaffolding in fission yeast cytokinesis. J Cell Sci. 2012;125:2973-85 pubmed publisher
    ..Collectively, our study recognizes domains regulating Mid1 cortical localization and reveals domains sufficient for contractile-ring assembly. ..

More Information

Publications22

  1. Salimova E, Sohrmann M, Fournier N, Simanis V. The S. pombe orthologue of the S. cerevisiae mob1 gene is essential and functions in signalling the onset of septum formation. J Cell Sci. 2000;113 ( Pt 10):1695-704 pubmed
    ..We also demonstrate that mob1p can be precipitated from cells in a complex with the septation regulating kinase sid2p. ..
  2. Wu J, Kuhn J, Kovar D, Pollard T. Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis. Dev Cell. 2003;5:723-34 pubmed
    ..Ring contraction and disassembly begin 37 min after SPB separation. This spatial and temporal hierarchy provides the framework for analysis of molecular mechanisms. ..
  3. Almonacid M, Celton Morizur S, Jakubowski J, Dingli F, Loew D, Mayeux A, et al. Temporal control of contractile ring assembly by Plo1 regulation of myosin II recruitment by Mid1/anillin. Curr Biol. 2011;21:473-9 pubmed publisher
    ..phosphorylates several residues within the first 100 amino acids of Mid1, which directly interact with the IQGAP Rng2, and influences the timing of myosin II recruitment...
  4. Pinar M, Coll P, Rincon S, Perez P. Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis. Mol Biol Cell. 2008;19:1727-38 pubmed publisher
    ..Together, these data suggest that Pxl1 modulates Rho1 GTPase signaling and plays a role in the formation and contraction of the actomyosin ring during cytokinesis. ..
  5. Kovar D, Wu J, Pollard T. Profilin-mediated competition between capping protein and formin Cdc12p during cytokinesis in fission yeast. Mol Biol Cell. 2005;16:2313-24 pubmed
    ..Given that capping protein arrives at the division site 20 min later than Cdc12p, capping protein may slowly replace Cdc12p on filament barbed ends in preparation for filament disassembly during ring constriction. ..
  6. Arasada R, Pollard T. A role for F-BAR protein Rga7p during cytokinesis in S. pombe. J Cell Sci. 2015;128:2259-68 pubmed publisher
    ..The late arrival and lower than normal numbers of Bgs4p result in septal defects late in cytokinesis, and in the lysis of separating cells, similar to that in cells with mutations in the cwg1(+) gene (which encodes Bgs4p). ..
  7. Mata J. Genome-wide mapping of myosin protein-RNA networks suggests the existence of specialized protein production sites. FASEB J. 2010;24:479-84 pubmed publisher
    ..I propose that these complexes represent specialized sites for the production of myosin proteins and the assembly of cytoskeletal components, respectively. ..
  8. Nakano K, Mabuchi I. Actin-capping protein is involved in controlling organization of actin cytoskeleton together with ADF/cofilin, profilin and F-actin crosslinking proteins in fission yeast. Genes Cells. 2006;11:893-905 pubmed
    ..In addition, CP is likely involved in assembling the F-actin contractile ring and F-actin patch with F-actin-crosslinking proteins. ..
  9. Tebbs I, Pollard T. Separate roles of IQGAP Rng2p in forming and constricting the Schizosaccharomyces pombe cytokinetic contractile ring. Mol Biol Cell. 2013;24:1904-17 pubmed publisher
    ..The Rng2p C-terminal domain is the only domain essential for viability. Our studies identified several distinct functions of Rng2 at multiple stages of cytokinesis.
  10. Goss J, Kim S, Bledsoe H, Pollard T. Characterization of the roles of Blt1p in fission yeast cytokinesis. Mol Biol Cell. 2014;25:1946-57 pubmed publisher
    ..We find three defects that likely contribute to this delay. Without Blt1p, contractile rings recruited and retained less Sid2p/Mob1p and Clp1p phosphatase, and ?-glucan synthase Bgs1p accumulated slowly at the cleavage site. ..
  11. Nakano K, Satoh K, Morimatsu A, Ohnuma M, Mabuchi I. Interactions among a fimbrin, a capping protein, and an actin-depolymerizing factor in organization of the fission yeast actin cytoskeleton. Mol Biol Cell. 2001;12:3515-26 pubmed
    ..Finally, genetic studies suggested that Fim1 may function in cytokinesis in cooperation with Cdc15 (PSTPIP) and Rng2 (IQGAP), respectively.
  12. Balasubramanian M, McCollum D, Chang L, Wong K, Naqvi N, He X, et al. Isolation and characterization of new fission yeast cytokinesis mutants. Genetics. 1998;149:1265-75 pubmed
    ..In addition, we have characterized a phenotype that has not been described previously in cytokinesis mutants, namely the failure to reorganize actin patches to the medial region of the cell in preparation for septum formation. ..
  13. Coffman V, Sees J, Kovar D, Wu J. The formins Cdc12 and For3 cooperate during contractile ring assembly in cytokinesis. J Cell Biol. 2013;203:101-14 pubmed publisher
    ..Together, these data indicate that different formins cooperate in cytokinesis and that de novo actin assembly at the division site is predominant for contractile ring formation. ..