Gene Symbol: rhp6
Description: ubiquitin-conjugating enzyme E2 2
Alias: sng1, ubc2, ubiquitin-conjugating enzyme E2 2
Species: fission yeast

Top Publications

  1. Verkade H, Teli T, Laursen L, Murray J, O Connell M. A homologue of the Rad18 postreplication repair gene is required for DNA damage responses throughout the fission yeast cell cycle. Mol Genet Genomics. 2001;265:993-1003 pubmed
  2. Naresh A, Saini S, Singh J. Identification of Uhp1, a ubiquitinated histone-like protein, as a target/mediator of Rhp6 in mating-type silencing in fission yeast. J Biol Chem. 2003;278:9185-94 pubmed
    ..In addition, DNA repair gene rhp6, which plays a role in post-replication DNA repair and ubiquitination of proteins including histones, is also ..
  3. Takeda K, Yanagida M. Regulation of nuclear proteasome by Rhp6/Ubc2 through ubiquitination and destruction of the sensor and anchor Cut8. Cell. 2005;122:393-405 pubmed
    ..Here we show that the fission yeast ubiquitin-conjugating Rhp6/Ubc2/Rad6 and ligating enzymes Ubr1 are responsible for nuclear enrichment of proteasome through the function of Cut8, a ..
  4. Zofall M, Grewal S. HULC, a histone H2B ubiquitinating complex, modulates heterochromatin independent of histone methylation in fission yeast. J Biol Chem. 2007;282:14065-72 pubmed
    ..In this study, we report the biochemical and genetic characterization of a ubiquitin-conjugating enzyme Rhp6 (a homolog of budding yeast Rad6), which has been shown to negatively affect stability of heterochromatic ..
  5. Dul B, Walworth N. The plant homeodomain fingers of fission yeast Msc1 exhibit E3 ubiquitin ligase activity. J Biol Chem. 2007;282:18397-406 pubmed
    ..Msc1 co-precipitates Rhp6, the S. pombe homologue of the human ubiquitin-conjugating enzyme Ubc2...
  6. Nielsen I, Nielsen O, Murray J, Thon G. The fission yeast ubiquitin-conjugating enzymes UbcP3, Ubc15, and Rhp6 affect transcriptional silencing of the mating-type region. Eukaryot Cell. 2002;1:613-25 pubmed
    ..of cDNAs encoding the putative E2 ubiquitin-conjugating enzymes UbcP3, Ubc15 (ubiquitin-conjugating enzyme), or Rhp6 (Rad homolog pombe) from the strong nmt1 promoter derepressed the silent mating-type loci mat2 and mat3 and ..
  7. Lv Z, Rickman K, Yuan L, Williams K, Selvam S, Woosley A, et al. S. pombe Uba1-Ubc15 Structure Reveals a Novel Regulatory Mechanism of Ubiquitin E2 Activity. Mol Cell. 2017;65:699-714.e6 pubmed publisher
  8. Ramalingam A, Prendergast G. Bin1 homolog hob1 supports a Rad6-Set1 pathway of transcriptional repression in fission yeast. Cell Cycle. 2007;6:1655-62 pubmed
    ..Taken together, these findings suggest that hob1+ may support a mechanism of transcriptional repression possibly relevant to the role of Bin1 in cancer suppression. ..
  9. Choi E, Kim H, Jang Y, Hong S, Park S. Two ubiquitin-conjugating enzymes, Rhp6 and UbcX, regulate heterochromatin silencing in Schizosaccharomyces pombe. Mol Cell Biol. 2002;22:8366-74 pubmed
    ..Interestingly, we identified two genes, rhp6(+) and ubcX(+) (ubiquitin-conjugating enzyme participating in silencing), both of which encode ubiquitin-..

More Information


  1. Doe C, Whitby M. The involvement of Srs2 in post-replication repair and homologous recombination in fission yeast. Nucleic Acids Res. 2004;32:1480-91 pubmed
    ..In addition to acting as an antirecombinase, we also show that Srs2 can aid the recombinational repair of camptothecin-induced collapsed replication forks, independently of PRR. ..
  2. Olsen S, Lima C. Structure of a ubiquitin E1-E2 complex: insights to E1-E2 thioester transfer. Mol Cell. 2013;49:884-96 pubmed publisher
    ..Comparison to a Ub E1/Ub/ATP·Mg structure reveals conformational changes in the E1 that bring the E1 and E2 active sites together...
  3. Penney M, Samejima I, Wilkinson C, McInerny C, Mathiassen S, Wallace M, et al. Fission yeast 26S proteasome mutants are multi-drug resistant due to stabilization of the Pap1 transcription factor. PLoS ONE. 2012;7:e50796 pubmed publisher
    ..We show that the ubiquitylation and ultimately the degradation of Pap1 depend on the Rhp6/Ubc2 E2 ubiquitin conjugating enzyme and the Ubr1 E3 ubiquitin-protein ligase...
  4. Reynolds P, Koken M, Hoeijmakers J, Prakash S, Prakash L. The rhp6+ gene of Schizosaccharomyces pombe: a structural and functional homolog of the RAD6 gene from the distantly related yeast Saccharomyces cerevisiae. EMBO J. 1990;9:1423-30 pubmed
    ..Here, we show that RAD6 and the rhp6+ gene from the distantly related yeast Schizosaccharomyces pombe share a high degree of structural and functional ..
  5. Kitamura K, Katayama S, Dhut S, Sato M, Watanabe Y, Yamamoto M, et al. Phosphorylation of Mei2 and Ste11 by Pat1 kinase inhibits sexual differentiation via ubiquitin proteolysis and 14-3-3 protein in fission yeast. Dev Cell. 2001;1:389-99 pubmed
    ..Mei2 is degraded via a ubiquitin-proteasome pathway in a phosphorylation-dependent fashion. The E2 Ubc2 and the E3 Ubr1 are required for this proteolysis...
  6. Singh J, Goel V, Klar A. A novel function of the DNA repair gene rhp6 in mating-type silencing by chromatin remodeling in fission yeast. Mol Cell Biol. 1998;18:5511-22 pubmed
    ..pombe as well as of S. cerevisiae. This unique mutant phenotype defined a locus which we named sng1 (for silencing not governed)...
  7. Osaka F, Seino H, Seno T, Yamao F. A ubiquitin-conjugating enzyme in fission yeast that is essential for the onset of anaphase in mitosis. Mol Cell Biol. 1997;17:3388-97 pubmed
  8. Tanny J, Erdjument Bromage H, Tempst P, Allis C. Ubiquitylation of histone H2B controls RNA polymerase II transcription elongation independently of histone H3 methylation. Genes Dev. 2007;21:835-47 pubmed
    ..We propose that ubiquitylation of H2B impacts transcription elongation and nuclear architecture through its effects on chromatin dynamics. ..
  9. Lee C, Yeh T, Hughes B, Espenshade P. Regulation of the Sre1 hypoxic transcription factor by oxygen-dependent control of DNA binding. Mol Cell. 2011;44:225-34 pubmed publisher
    ..In contrast to the HIF system, where proline hydroxylation is essential for regulation, Ofd1 inhibition of Sre1N does not require hydroxylation and, thus, defines a new mechanism for hypoxic gene regulation. ..
  10. Rowley R, Zhang J. Caffeine-mediated override of checkpoint controls. A requirement for rhp6 (Schizosaccharomyces pombe). Genetics. 1999;152:61-71 pubmed
    ..Mutants 788 and 1176 are point mutants of rhp6, the fission yeast homolog of the budding yeast RAD6 gene...