Gene Symbol: ran1
Description: serine/threonine protein kinase Ran1
Alias: pat1, serine/threonine protein kinase Ran1
Species: fission yeast

Top Publications

  1. Sipiczki M. The role of sterility genes (ste and aff) in the initiation of sexual development in Schizosaccharomyces pombe. Mol Gen Genet. 1988;213:529-34 pubmed
    ..I found that the ste1, ste3 and ste8 genes act very early in the sexual development, presumably before the pat1-controlled conjugation-specific event...
  2. McLeod M, Beach D. A specific inhibitor of the ran1+ protein kinase regulates entry into meiosis in Schizosaccharomyces pombe. Nature. 1988;332:509-14 pubmed
    ..acts as a critical meiotic inducer by binding non-covalently to a newly identified protein kinase encoded by the ran1+ gene and inhibiting its enzymatic activity...
  3. Watanabe Y, Shinozaki Yabana S, Chikashige Y, Hiraoka Y, Yamamoto M. Phosphorylation of RNA-binding protein controls cell cycle switch from mitotic to meiotic in fission yeast. Nature. 1997;386:187-90 pubmed
    ..In the fission yeast Schizosaccharomyces pombe, inactivation of the protein kinase Pat1(Ran1) upon nutrient deprivation triggers entry into the meiotic cell cycle...
  4. Shiozaki K, Russell P. Conjugation, meiosis, and the osmotic stress response are regulated by Spc1 kinase through Atf1 transcription factor in fission yeast. Genes Dev. 1996;10:2276-88 pubmed
    ..However, unlike spc1- mutants, atf1- cells have no mitotic cell-cycle defect, indicating that the stress response pathway bifurcates at Spc1 to regulate independently meiosis and mitosis. ..
  5. Li P, McLeod M. Molecular mimicry in development: identification of ste11+ as a substrate and mei3+ as a pseudosubstrate inhibitor of ran1+ kinase. Cell. 1996;87:869-80 pubmed
    b>ran1+ (pat1+) kinase inhibits exit from the mitotic cell cycle and entry into meiosis. Inactivation of ran1+ by mei3+ is sufficient to precipitate the entire meiotic developmental program...
  6. Murakami H, Nurse P. Meiotic DNA replication checkpoint control in fission yeast. Genes Dev. 1999;13:2581-93 pubmed
    ..This second checkpoint is meiosis specific and may reflect processes occurring only during meiosis such as increased recombination rates, an extended duration of nuclear division, or homolog chromosome pairing. ..
  7. Beach D. Sexual differentiation is controlled by a protein kinase encoded by the ran1+ gene in fission yeast. Cold Spring Harb Symp Quant Biol. 1985;50:635-41 pubmed
  8. Wang W, Li P, Schettino A, Peng Z, McLeod M. Characterization of functional regions in the Schizosaccharomyces pombe mei3 developmental activator. Genetics. 1998;150:1007-18 pubmed
    ..Ectopic expression of mei3(+) in haploid cells causes meiotic catastrophe. Mei3 is an inhibitor of Ran1/Pat1 kinase and contains a nine-amino-acid motif, Mei3-RKDIII, that resembles two regions in the Ste11 substrate ..
  9. Nakashima A, Hasegawa T, Mori S, Ueno M, Tanaka S, Ushimaru T, et al. A starvation-specific serine protease gene, isp6+, is involved in both autophagy and sexual development in Schizosaccharomyces pombe. Curr Genet. 2006;49:403-13 pubmed
    ..b>Pat1 protein kinase negatively controls meiosis, and a temperature-sensitive mutant of pat1, pat1-114, initiates meiosis ..

More Information


  1. Obara Ishihara T, Okayama H. A B-type cyclin negatively regulates conjugation via interacting with cell cycle 'start' genes in fission yeast. EMBO J. 1994;13:1863-72 pubmed
    ..The Pat1 kinase plays a pivotal role in switching between vegetative growth and sexual development, and its inactivation in ..
  2. Stettler S, Warbrick E, Prochnik S, Mackie S, Fantes P. The wis1 signal transduction pathway is required for expression of cAMP-repressed genes in fission yeast. J Cell Sci. 1996;109 ( Pt 7):1927-35 pubmed
    ..loss of wis1 function leads to defective conjugation, and to suppression of the hyperconjugation phenotype of the pat1-114 mutation...
  3. Yamamoto A, Hiraoka Y. Monopolar spindle attachment of sister chromatids is ensured by two distinct mechanisms at the first meiotic division in fission yeast. EMBO J. 2003;22:2284-96 pubmed
    ..This monopolar attachment requires both mating pheromone signaling and inactivation of Pat1 kinase (a key negative regulator of meiosis)...
  4. McLeod M, Shor B, Caporaso A, Wang W, Chen H, Hu L. Cpc2, a fission yeast homologue of mammalian RACK1 protein, interacts with Ran1 (Pat1) kinase To regulate cell cycle progression and meiotic development. Mol Cell Biol. 2000;20:4016-27 pubmed
    The Schizosaccharomyces pombe ran1/pat1 gene regulates the transition between mitosis and meiosis. Inactivation of Ran1 (Pat1) kinase is necessary and sufficient for cells to exit the cell cycle and undergo meiosis...
  5. Mach K, Cheng Q, Albright C. ras1 and pat1 alleles interact to quantitatively and qualitatively alter conjugation in fission yeast. Curr Genet. 1998;34:172-82 pubmed
    ..A novel allele of pat1, pat1-e1, was isolated that increases the mating of ras1-D43E mutants to near wild-type levels but does not ..
  6. Amorim M, Cotobal C, Duncan C, Mata J. Global coordination of transcriptional control and mRNA decay during cellular differentiation. Mol Syst Biol. 2010;6:380 pubmed publisher
    ..Our data provide insight into the topology of regulatory networks integrating transcriptional and posttranscriptional controls. ..
  7. Ayte J, Schweitzer C, Zarzov P, Nurse P, DeCaprio J. Feedback regulation of the MBF transcription factor by cyclin Cig2. Nat Cell Biol. 2001;3:1043-50 pubmed
    ..Cig2p can bind to Res2p, promote the phosphorylation of Res1p and inhibit MBF-dependent gene transcription. Cig2p thus forms an autoregulating feedback-inhibition loop with MBF which is important for normal regulation of the cell cycle. ..
  8. Wurm J, Overbeck J, Sprangers R. The S. pombe mRNA decapping complex recruits cofactors and an Edc1-like activator through a single dynamic surface. RNA. 2016;22:1360-72 pubmed publisher
    ..pombe Dcp1 can interact with proline-rich sequences in the exonuclease Xrn1, the scaffolding protein Pat1, the helicase Dhh1, and the C-terminal disordered region of Dcp2...
  9. Alvarez B, Moreno S. Fission yeast Tor2 promotes cell growth and represses cell differentiation. J Cell Sci. 2006;119:4475-85 pubmed
    ..Thus, our data reveal a unique regulatory function of the Tor pathway - ensuring that growth and cell differentiation become mutually exclusive and that the choice between them depends on environmental conditions. ..
  10. Oowatari Y, Jeong H, Tanae K, Nakagawa T, Kawamukai M. Regulation and role of an RNA-binding protein Msa2 in controlling the sexual differentiation of fission yeast. Curr Genet. 2011;57:191-200 pubmed publisher
    ..mutants for their ability to inhibit sexual differentiation and their suppressive effect on a temperature sensitive pat1 mutant...
  11. Forsburg S, Nurse P. Analysis of the Schizosaccharomyces pombe cyclin puc1: evidence for a role in cell cycle exit. J Cell Sci. 1994;107 ( Pt 3):601-13 pubmed
    ..We conclude that puc1 contributes to negative regulation of the timing of sexual development in fission yeast, and functions at the transition between cycling and non-cycling cells. ..
  12. Davey J, Nielsen O. Mutations in cyr1 and pat1 reveal pheromone-induced G1 arrest in the fission yeast Schizosaccharomyces pombe. Curr Genet. 1994;26:105-12 pubmed
    ..The second mutant contains the temperature-sensitive pat1-114 allele...
  13. Matsuyama A, Yabana N, Watanabe Y, Yamamoto M. Schizosaccharomyces pombe Ste7p is required for both promotion and withholding of the entry to meiosis. Genetics. 2000;155:539-49 pubmed
    ..The temperature-sensitive pat1-114 mutant underwent ectopic conjugation at the semirestrictive temperature when its genetic background was ste7(+),..
  14. Sato M, Watanabe Y, Akiyoshi Y, Yamamoto M. 14-3-3 protein interferes with the binding of RNA to the phosphorylated form of fission yeast meiotic regulator Mei2p. Curr Biol. 2002;12:141-5 pubmed
    The switch from mitosis to meiosis is controlled by the Pat1(Ran1) kinase-Mei2p system in Schizosaccharomyces pombe...
  15. Zheng L, Schwartz C, Wee L, Oliferenko S. The fission yeast transforming acidic coiled coil-related protein Mia1p/Alp7p is required for formation and maintenance of persistent microtubule-organizing centers at the nuclear envelope. Mol Biol Cell. 2006;17:2212-22 pubmed
    ..When microtubule polymerization was allowed, MTOCs were efficiently assembled de novo. We propose a model where MTOC emergence is a self-organizing process requiring the continuous association of microtubules with nucleation sites. ..
  16. Krapp A, Collin P, Cokoja A, Dischinger S, Cano E, Simanis V. The Schizosaccharomyces pombe septation initiation network (SIN) is required for spore formation in meiosis. J Cell Sci. 2006;119:2882-91 pubmed
    ..The protein kinases Sid1p and Cdc7p do not associate with the spindle pole body until meiosis II, when forespore membrane deposition begins. These data indicate a role for the SIN in regulating spore formation during meiosis. ..
  17. DeVoti J, Seydoux G, Beach D, McLeod M. Interaction between ran1+ protein kinase and cAMP dependent protein kinase as negative regulators of fission yeast meiosis. EMBO J. 1991;10:3759-68 pubmed
    ..The mei3+ gene product acts as a meiotic inducer by binding to and inhibiting the ran1+ protein kinase. Inactivation of ran1+ kinase is both necessary and sufficient to allow meiotic differentiation...
  18. Win T, Draper S, Read R, Pearce J, Norbury C, Wang S. Requirement of fission yeast Cid14 in polyadenylation of rRNAs. Mol Cell Biol. 2006;26:1710-21 pubmed
    ..Polyadenylation-assisted nuclear RNA turnover is therefore likely to be a common eukaryotic mechanism affecting diverse biological processes. ..
  19. Otsubo Y, Yamashita A, Ohno H, Yamamoto M. S. pombe TORC1 activates the ubiquitin-proteasomal degradation of the meiotic regulator Mei2 in cooperation with Pat1 kinase. J Cell Sci. 2014;127:2639-46 pubmed publisher
    ..Based on these data, we propose that TORC1 contributes to the suppression of sexual development by phosphorylating Mei2, in addition to controlling the cellular metabolic status. ..
  20. Nielsen O, Egel R. The pat1 protein kinase controls transcription of the mating-type genes in fission yeast. EMBO J. 1990;9:1401-6 pubmed
    ..Conjugation can also be induced in pat1-ts mutants by a shift to a semi-permissive temperature...
  21. Sukegawa Y, Yamashita A, Yamamoto M. The fission yeast stress-responsive MAPK pathway promotes meiosis via the phosphorylation of Pol II CTD in response to environmental and feedback cues. PLoS Genet. 2011;7:e1002387 pubmed publisher
    ..This study also demonstrates clearly that the stress-responsive MAP kinase pathway can modulates gene expression through phosphorylation of Pol II CTD. ..
  22. Zhang M, Wu P, Kelly F, Nurse P, Hang H. Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast. PLoS Biol. 2013;11:e1001597 pubmed publisher
    ..We propose that control of palmitoyltransferase activity levels provides a fundamental mechanism for modulating palmitoylomes and cellular functions. ..
  23. Lino Y, Yamamoto M. Mutants of Schizosaccharomyces pombe which sporulate in the haploid state. Mol Gen Genet. 1985;198:416-21 pubmed
    ..The mutants are all recessive and define a single gene pat1. The pat1 gene maps very close to the centromere of chromosome II...
  24. Shinozaki Yabana S, Watanabe Y, Yamamoto M. Novel WD-repeat protein Mip1p facilitates function of the meiotic regulator Mei2p in fission yeast. Mol Cell Biol. 2000;20:1234-42 pubmed
    ..These results suggest that Mip1p assists functional expression of a number of proteins required for proliferation and sexual development in fission yeast. ..
  25. Tanabe K, Ito N, Wakuri T, Ozoe F, Umeda M, Katayama S, et al. Sla1, a Schizosaccharomyces pombe homolog of the human La protein, induces ectopic meiosis when its C terminus is truncated. Eukaryot Cell. 2003;2:1274-87 pubmed
    ..Strikingly, Sla1DeltaC induced haploid meiosis in a heterothallic strain, similar to the pat1-114 or mei2-SATA mutation...
  26. Willer M, Hoffmann L, Styrkarsdottir U, Egel R, Davey J, Nielsen O. Two-step activation of meiosis by the mat1 locus in Schizosaccharomyces pombe. Mol Cell Biol. 1995;15:4964-70 pubmed
    ..The mat1-Pm and mat1-Mm products complete the initiation of meiosis by activating transcription of the mei3 gene. ..
  27. Rotondo G, Gillespie M, Frendewey D. Rescue of the fission yeast snRNA synthesis mutant snm1 by overexpression of the double-strand-specific Pac1 ribonuclease. Mol Gen Genet. 1995;247:698-708 pubmed
    ..The pac1+ gene was previously identified as a suppressor of the ran1 mutant and by its ability to cause sterility when overexpressed...
  28. Iino Y, Sugimoto A, Yamamoto M. S. pombe pac1+, whose overexpression inhibits sexual development, encodes a ribonuclease III-like RNase. EMBO J. 1991;10:221-6 pubmed
    The Schizosaccharomyces pombe pac1 gene is a multicopy suppressor of the pat1 temperature-sensitive mutation, which directs uncontrolled meiosis at the restrictive temperature...