Gene Symbol: rad26
Description: ATR checkpoint kinase regulatory subunit Rad26
Alias: ATR checkpoint kinase regulatory subunit Rad26
Species: fission yeast

Top Publications

  1. al Khodairy F, Fotou E, Sheldrick K, Griffiths D, Lehmann A, Carr A. Identification and characterization of new elements involved in checkpoint and feedback controls in fission yeast. Mol Biol Cell. 1994;5:147-60 pubmed
    ..Two mutants, rad26.T12 and rad27.T15, were selected for molecular analysis. The null allele of the rad26 gene (rad26...
  2. Lindsay H, Griffiths D, Edwards R, Christensen P, Murray J, Osman F, et al. S-phase-specific activation of Cds1 kinase defines a subpathway of the checkpoint response in Schizosaccharomyces pombe. Genes Dev. 1998;12:382-95 pubmed
    ..S phase is dependent on all six checkpoint Rad proteins, and Cds1 interacts both genetically and physically with Rad26. Unlike its Saccharomyces cerevisiae counterpart Rad53, Cds1 is not required for the mitotic arrest checkpoints ..
  3. Taricani L, Wang T. Rad4TopBP1, a scaffold protein, plays separate roles in DNA damage and replication checkpoints and DNA replication. Mol Biol Cell. 2006;17:3456-68 pubmed
  4. Matsuura A, Naito T, Ishikawa F. Genetic control of telomere integrity in Schizosaccharomyces pombe: rad3(+) and tel1(+) are parts of two regulatory networks independent of the downstream protein kinases chk1(+) and cds1(+). Genetics. 1999;152:1501-12 pubmed
    ..Genetic analysis revealed that the other checkpoint rad mutations rad1, rad17, and rad26 belong to the same phenotypic class with rad3 with regard to control of the telomere length...
  5. Edwards R, Bentley N, Carr A. A Rad3-Rad26 complex responds to DNA damage independently of other checkpoint proteins. Nat Cell Biol. 1999;1:393-8 pubmed
    ..Here we report a stable association between Rad3 and Rad26 in soluble protein extracts. Rad26 shows Rad3-dependent phosphorylation after DNA damage...
  6. Moser B, Subramanian L, Chang Y, Noguchi C, Noguchi E, Nakamura T. Differential arrival of leading and lagging strand DNA polymerases at fission yeast telomeres. EMBO J. 2009;28:810-20 pubmed publisher
    ..Trt1, Taz1, Rap1, Pot1 and Stn1), DNA replication proteins (DNA polymerases, MCM, RPA), checkpoint protein Rad26 and DNA repair protein Nbs1 to telomeres...
  7. Hermand D, Nurse P. Cdc18 enforces long-term maintenance of the S phase checkpoint by anchoring the Rad3-Rad26 complex to chromatin. Mol Cell. 2007;26:553-63 pubmed
    ..when DNA replication stalls, Cdc18 persists in a chromatin-bound complex including the checkpoint kinases Rad3 and Rad26. Rad26 directly binds Cdc18 and is required for Rad3 recruitment to chromatin...
  8. Nakamura T, Moser B, Russell P. Telomere binding of checkpoint sensor and DNA repair proteins contributes to maintenance of functional fission yeast telomeres. Genetics. 2002;161:1437-52 pubmed
    ..basis of telomere lengths of mutant combinations of various checkpoint-related proteins (Rad1, Rad3, Rad9, Rad17, Rad26, Hus1, Crb2, Chk1, Cds1), Tel1, a telomere-binding protein (Taz1), and DNA repair proteins (Ku70, Rad32), we ..
  9. Griffiths D, Barbet N, McCready S, Lehmann A, Carr A. Fission yeast rad17: a homologue of budding yeast RAD24 that shares regions of sequence similarity with DNA polymerase accessory proteins. EMBO J. 1995;14:5812-23 pubmed
    ..Six of these (rad1, rad3, rad9, rad17, rad26 and hus1) are also required for the checkpoint which prevents mitosis from occurring before DNA replication is ..

More Information


  1. Harris S, Kemplen C, Caspari T, Chan C, Lindsay H, Poitelea M, et al. Delineating the position of rad4+/cut5+ within the DNA-structure checkpoint pathways in Schizosaccharomyces pombe. J Cell Sci. 2003;116:3519-29 pubmed
    ..Phosphorylation of Rad9, Rad26 and Hus1 in response to either DNA damage or inhibition of DNA replication are independent of Rad4/Cut5 checkpoint ..
  2. Wolkow T, Enoch T. Fission yeast Rad26 responds to DNA damage independently of Rad3. BMC Genet. 2003;4:6 pubmed
    The Rad26/Rad3 complex in fission yeast detects genotoxic insults and initiates the cell cycle arrest and recovery activities of the DNA damage checkpoint...
  3. Saitoh S, Chabes A, McDonald W, Thelander L, Yates J, Russell P. Cid13 is a cytoplasmic poly(A) polymerase that regulates ribonucleotide reductase mRNA. Cell. 2002;109:563-73 pubmed
    ..cid13 mutants have reduced dNTP pools and are sensitive to hydroxyurea, an RNR inhibitor. We propose that Cid13 defines a cytoplasmic form of poly(A) polymerase important for DNA replication and genome maintenance. ..
  4. Williams D, McIntosh J. mcl1+, the Schizosaccharomyces pombe homologue of CTF4, is important for chromosome replication, cohesion, and segregation. Eukaryot Cell. 2002;1:758-73 pubmed
    ..It interacts genetically with replication components and causes an S-phase delay when overexpressed. We propose that Mcl1p, like Ctf4p, has a role in regulating DNA replication complexes. ..
  5. Herring M, Davenport N, Stephan K, Campbell S, White R, Kark J, et al. Fission yeast Rad26ATRIP delays spindle-pole-body separation following interphase microtubule damage. J Cell Sci. 2010;123:1537-45 pubmed publisher
    The conserved fission yeast protein Rad26(ATRIP) preserves genomic stability by occupying central positions within DNA-structure checkpoint pathways...
  6. Yin L, Locovei A, D URSO G. Activation of the DNA damage checkpoint in mutants defective in DNA replication initiation. Mol Biol Cell. 2008;19:4374-82 pubmed publisher
  7. Khair L, Chang Y, Subramanian L, Russell P, Nakamura T. Roles of the checkpoint sensor clamp Rad9-Rad1-Hus1 (911)-complex and the clamp loaders Rad17-RFC and Ctf18-RFC in Schizosaccharomyces pombe telomere maintenance. Cell Cycle. 2010;9:2237-48 pubmed
    ..Since checkpoint sensor proteins are highly conserved, genetic redundancies uncovered here may be relevant to telomere maintenance and detection of DNA damage in other eukaryotes. ..
  8. Rapp J, Noguchi C, Das M, Wong L, Ansbach A, Holmes A, et al. Checkpoint-dependent and -independent roles of Swi3 in replication fork recovery and sister chromatid cohesion in fission yeast. PLoS ONE. 2010;5:e13379 pubmed publisher
  9. Liu V, Bhaumik D, Wang T. Mutator phenotype induced by aberrant replication. Mol Cell Biol. 1999;19:1126-35 pubmed
    ..replication mutators all exhibited decreased viability in combination with deletion of a checkpoint Rad protein, Rad26. Interestingly, deletion of Cds1, a protein kinase functioning in a checkpoint Rad-mediated reversible S-phase ..
  10. Subramanian L, Nakamura T. A kinase-independent role for the Rad3(ATR)-Rad26(ATRIP) complex in recruitment of Tel1(ATM) to telomeres in fission yeast. PLoS Genet. 2010;6:e1000839 pubmed publisher
    ..cells, which lack the C-terminal 60 amino acid Tel1(ATM) interaction domain of Nbs1, was dependent on Rad3(ATR)-Rad26(ATRIP), but the kinase domain of Rad3(ATR) was dispensable...
  11. Katayama S, Kitamura K, Lehmann A, Nikaido O, Toda T. Fission yeast F-box protein Pof3 is required for genome integrity and telomere function. Mol Biol Cell. 2002;13:211-24 pubmed
    ..The results highlight a role of the SCF(Pof3) ubiquitin ligase in genome integrity via maintaining chromatin structures. ..
  12. Yoshida S, Al Amodi H, Nakamura T, McInerny C, Shimoda C. The Schizosaccharomyces pombe cdt2(+) gene, a target of G1-S phase-specific transcription factor complex DSC1, is required for mitotic and premeiotic DNA replication. Genetics. 2003;164:881-93 pubmed
    ..These observations suggest that cdt2(+) plays an important role in DNA replication in both the mitotic and the meiotic life cycles of fission yeast. ..
  13. Win T, Goodwin A, Hickson I, Norbury C, Wang S. Requirement for Schizosaccharomyces pombe Top3 in the maintenance of chromosome integrity. J Cell Sci. 2004;117:4769-78 pubmed
    ..Our data are consistent with the notion that Top3 has a key function in homologous recombinational repair during S phase that is essential for ensuring subsequent fidelity of chromosome segregation. ..
  14. Yue M, Zeng L, Singh A, Xu Y. Rad4 mainly functions in Chk1-mediated DNA damage checkpoint pathway as a scaffold protein in the fission yeast Schizosaccharomyces pombe. PLoS ONE. 2014;9:e92936 pubmed publisher
    ..It plays a minor role or is functionally redundant with an unknown factor in Rad3 activation. ..
  15. Akai Y, Kurokawa Y, Nakazawa N, Tonami Murakami Y, Suzuki Y, Yoshimura S, et al. Opposing role of condensin hinge against replication protein A in mitosis and interphase through promoting DNA annealing. Open Biol. 2011;1:110023 pubmed publisher
    ..We show that 16 suppressor mutations of cut14-Y1 were all mapped within the hinge domain, which surrounded the original L543 mutation site. ..
  16. Du L, Moser B, Russell P. Homo-oligomerization is the essential function of the tandem BRCT domains in the checkpoint protein Crb2. J Biol Chem. 2004;279:38409-14 pubmed
    ..These results demonstrate that the crucial function of the tandem BRCT domains is to oligomerize Crb2. ..
  17. Kai M, Wang T. Checkpoint activation regulates mutagenic translesion synthesis. Genes Dev. 2003;17:64-76 pubmed
    ..Moreover, association of DinB with chromatin is dependent on functional Rad17, and DinB physically interacts with the checkpoint-clamp components Hus1 and Rad1. Thus, translesion synthesis is a part of the checkpoint response. ..
  18. Baschal E, Chen K, Elliott L, Herring M, Verde S, Wolkow T. The fission yeast DNA structure checkpoint protein Rad26ATRIP/LCD1/UVSD accumulates in the cytoplasm following microtubule destabilization. BMC Cell Biol. 2006;7:32 pubmed
    ..In fission yeast, the Rad3 PIKK and its regulatory subunit Rad26 coordinate the detection of checkpoint signals with pathway outputs...
  19. Arroyo M, Wang T. Mutant PCNA alleles are associated with cdc phenotypes and sensitivity to DNA damage in fission yeast. Mol Gen Genet. 1998;257:505-18 pubmed
    ..A model for how pcn1-3 defects may affect DNA repair and replication is presented. ..
  20. Uchiyama M, Galli I, Griffiths D, Wang T. A novel mutant allele of Schizosaccharomyces pombe rad26 defective in monitoring S-phase progression to prevent premature mitosis. Mol Cell Biol. 1997;17:3103-15 pubmed
    ..However, this mutant is sensitive to neither UV nor gamma irradiation. Two genomic clones, rad26+ and cds1+, were found to suppress the hydroxyurea sensitivity of the aya14 mutant...
  21. Tanaka K, Russell P. Cds1 phosphorylation by Rad3-Rad26 kinase is mediated by forkhead-associated domain interaction with Mrc1. J Biol Chem. 2004;279:32079-86 pubmed
    ..Mrc1 (mediator of the replication checkpoint-1) and Rad3-Rad26 kinase are required for Cds1 activation, but exactly how Mrc1 mediates Cds1 activation is unknown...
  22. Synnes M, Nilssen E, Boye E, Grallert B. A novel chk1-dependent G1/M checkpoint in fission yeast. J Cell Sci. 2002;115:3609-18 pubmed
    ..We also show that commitment to cutting occurs already in early G1 phase. ..
  23. Tan S, Wang T. Analysis of fission yeast primase defines the checkpoint responses to aberrant S phase initiation. Mol Cell Biol. 2000;20:7853-66 pubmed
    ..Thus, the Cds1-mediated intra-S phase checkpoint response induced by hydroxyurea can also be distinguished from the S-M phase checkpoint response that requires the initiation DNA synthesis by Polalpha. ..
  24. Takahashi S, Kontani K, Araki Y, Katada T. Caf1 regulates translocation of ribonucleotide reductase by releasing nucleoplasmic Spd1-Suc22 assembly. Nucleic Acids Res. 2007;35:1187-97 pubmed
    ..Taken together, these results suggest a novel function of Caf1 as a key regulator in the stress-induced RNR activation. ..
  25. Smeets M, Francesconi S, Baldacci G. High dosage Rhp51 suppression of the MMS sensitivity of DNA structure checkpoint mutants reveals a relationship between Crb2 and Rhp51. Genes Cells. 2003;8:573-86 pubmed
    ..We conclude that fission yeast Crb2 and Rhp51 function in two parallel, tightly connected and coordinately regulated pathways. ..
  26. Kanoh J, Francesconi S, Collura A, Schramke V, Ishikawa F, Baldacci G, et al. The fission yeast spSet1p is a histone H3-K4 methyltransferase that functions in telomere maintenance and DNA repair in an ATM kinase Rad3-dependent pathway. J Mol Biol. 2003;326:1081-94 pubmed
    ..DNA damage checkpoint rad mutant cells, but not in cells lacking the ATM kinase Rad3 or its associated protein Rad26. Interestingly, spset1 deletion specifically causes a reduction in sensitivity to ultraviolet radiation of the ..
  27. Marchetti M, Kumar S, Hartsuiker E, Maftahi M, Carr A, Freyer G, et al. A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway. Proc Natl Acad Sci U S A. 2002;99:7472-7 pubmed
    ..These results suggest that, in fission yeast, the signal activating the intra-S-phase checkpoint is generated only when replication forks encounter DNA damage. ..
  28. Blaikley E, Tinline Purvis H, Kasparek T, Marguerat S, Sarkar S, Hulme L, et al. The DNA damage checkpoint pathway promotes extensive resection and nucleotide synthesis to facilitate homologous recombination repair and genome stability in fission yeast. Nucleic Acids Res. 2014;42:5644-56 pubmed publisher
    ..We propose that the DNA damage checkpoint pathway coordinates resection and nucleotide synthesis, thereby promoting efficient HR repair and genome stability. ..