Gene Symbol: prp4
Description: serine/threonine protein kinase Prp4
Alias: serine/threonine protein kinase Prp4
Species: fission yeast

Top Publications

  1. Potashkin J, Kim D, Fons M, Humphrey T, Frendewey D. Cell-division-cycle defects associated with fission yeast pre-mRNA splicing mutants. Curr Genet. 1998;34:153-63 pubmed
    ..Two are allelic with the previously described prp3 and prp4 mutations; the remaining four define the new alleles prp5-1, prp6-1, prp7-1, and prp9-1...
  2. Rosenberg G, Alahari S, Kaufer N. prp4 from Schizosaccharomyces pombe, a mutant deficient in pre-mRNA splicing isolated using genes containing artificial introns. Mol Gen Genet. 1991;226:305-9 pubmed
    ..With this approach three mutants were isolated which have a general defect in the splicing process. Two of these mutants fall into the prp1 complementation group and one defines a new complementation group, prp4.
  3. Gross T, Lützelberger M, Weigmann H, Klingenhoff A, Shenoy S, Kaufer N. Functional analysis of the fission yeast Prp4 protein kinase involved in pre-mRNA splicing and isolation of a putative mammalian homologue. Nucleic Acids Res. 1997;25:1028-35 pubmed
    The prp4 gene of Schizosaccharomyces pombe encodes a protein kinase. A physiological substrate is not yet known...
  4. Urushiyama S, Tani T, Ohshima Y. The prp1+ gene required for pre-mRNA splicing in Schizosaccharomyces pombe encodes a protein that contains TPR motifs and is similar to Prp6p of budding yeast. Genetics. 1997;147:101-15 pubmed
    ..These results suggest that Prp1p/Zer1p is either directly or indirectly involved in cell cycle progression and/or poly(A)+ RNA nuclear export, in addition to pre-mRNA splicing. ..
  5. Schwelnus W, Richert K, Opitz F, Gross T, Habara Y, Tani T, et al. Fission yeast Prp4p kinase regulates pre-mRNA splicing by phosphorylating a non-SR-splicing factor. EMBO Rep. 2001;2:35-41 pubmed
    ..In vivo labelling studies using a strain with a mutant allele of the prp4 gene in the genetic background indicate a change in phosphorylation of the Prp1p protein...
  6. Pozgajova M, Cipak L, Trakovická A. Prp4 kinase is required for proper segregation of chromosomes during meiosis in Schizosaccharomyces pombe. Acta Biochim Pol. 2013;60:871-3 pubmed
    ..Reversible phosphorylation of proteins plays a crucial role in this process. The Schizosaccharomyces pombe Prp4 is an essential serine/threonine protein kinase, which belongs to the Clk/Sty family...
  7. Gross T, Richert K, Mierke C, Lützelberger M, Kaufer N. Identification and characterization of srp1, a gene of fission yeast encoding a RNA binding domain and a RS domain typical of SR splicing factors. Nucleic Acids Res. 1998;26:505-11 pubmed
    ..of the mammalian SR splicing factor ASF/SF2 suppress the pre-mRNA splicing defect of the temperature-sensitive prp4-73 allele. prp4 encodes a protein kinase involved in pre-mRNA splicing...
  8. Schmidt H, Richert K, Drakas R, Kaufer N. spp42, identified as a classical suppressor of prp4-73, which encodes a kinase involved in pre-mRNA splicing in fission yeast, is a homologue of the splicing factor Prp8p. Genetics. 1999;153:1183-91 pubmed
    We have identified two classical extragenic suppressors, spp41 and spp42, of the temperature sensitive (ts) allele prp4-73. The prp4(+) gene of Schizosaccharomyces pombe encodes a protein kinase...
  9. Richert K, Schmidt H, Gross T, Kaufer F. The deubiquitinating enzyme Ubp21p of fission yeast stabilizes a mutant form of protein kinase Prp4p. Mol Genet Genomics. 2002;267:88-95 pubmed
    ..The results presented in this report are consistent with the notion that the deubiquitinating activity of Ubp21p may be involved in regulating the steady-state levels of proteins including Prp4p. ..

More Information


  1. Eckert D, Andrée N, Razanau A, Zock Emmenthal S, Lützelberger M, Plath S, et al. Prp4 Kinase Grants the License to Splice: Control of Weak Splice Sites during Spliceosome Activation. PLoS Genet. 2016;12:e1005768 pubmed publisher
    ..In this study, we further characterized another of these essential kinases, Prp4, and showed that the splicing of many introns is dependent on Prp4 kinase activity...
  2. Maddock J, Weidenhammer E, Adams C, Lunz R, Woolford J. Extragenic suppressors of Saccharomyces cerevisiae prp4 mutations identify a negative regulator of PRP genes. Genetics. 1994;136:833-47 pubmed
    The PRP4 gene encodes a protein that is a component of the U4/U6 small nuclear ribonucleoprotein particle and is necessary for both spliceosome assembly and pre-mRNA splicing...
  3. Alahari S, Schmidt H, Kaufer N. The fission yeast prp4+ gene involved in pre-mRNA splicing codes for a predicted serine/threonine kinase and is essential for growth. Nucleic Acids Res. 1993;21:4079-83 pubmed
    ..We exploited yeast genetics and identified and isolated the prp4 gene...
  4. Sajiki K, Hatanaka M, Nakamura T, Takeda K, Shimanuki M, Yoshida T, et al. Genetic control of cellular quiescence in S. pombe. J Cell Sci. 2009;122:1418-29 pubmed publisher
    ..We propose that the transcriptional role of Fcp1 is central in differentiating quiescence from proliferation. ..
  5. Lützelberger M, Bottner C, Schwelnus W, Zock Emmenthal S, Razanau A, Käufer N. The N-terminus of Prp1 (Prp6/U5-102 K) is essential for spliceosome activation in vivo. Nucleic Acids Res. 2010;38:1610-22 pubmed publisher
    ..the N-terminus, which prevent splicing to occur, include in vitro and in vivo identified phosphorylation sites of Prp4 kinase. These sites are highly conserved in the human ortholog U5-102 K...