Gene Symbol: pcn1
Description: DNA polymerase delta processivity factor
Alias: pcn, DNA polymerase delta processivity factor
Species: fission yeast

Top Publications

  1. Warbrick E, Lane D, Glover D, Cox L. A small peptide inhibitor of DNA replication defines the site of interaction between the cyclin-dependent kinase inhibitor p21WAF1 and proliferating cell nuclear antigen. Curr Biol. 1995;5:275-82 pubmed
    ..abstract truncated at 250 words) ..
  2. Zuo S, Gibbs E, Kelman Z, Wang T, O Donnell M, MacNeill S, et al. DNA polymerase delta isolated from Schizosaccharomyces pombe contains five subunits. Proc Natl Acad Sci U S A. 1997;94:11244-9 pubmed
    ..pombe chromosome 1. ..
  3. Tanaka H, Tanaka K, Murakami H, Okayama H. Fission yeast cdc24 is a replication factor C- and proliferating cell nuclear antigen-interacting factor essential for S-phase completion. Mol Cell Biol. 1999;19:1038-48 pubmed
    ..The temperature-sensitive cdc24 mutant is effectively rescued by pcn1(+), rfc1(+) (a fission yeast homologue of RFC1), and hhp1(+), which encode the proliferating cell nuclear antigen (..
  4. Reynolds N, Warbrick E, Fantes P, MacNeill S. Essential interaction between the fission yeast DNA polymerase delta subunit Cdc27 and Pcn1 (PCNA) mediated through a C-terminal p21(Cip1)-like PCNA binding motif. EMBO J. 2000;19:1108-18 pubmed
    ..We show that the 54 kDa subunit of DNA polymerase delta from Schizosaccharomyces pombe interacts directly with Pcn1 (PCNA) both in vivo and in vitro...
  5. Ramasubramanyan S, Coulon S, Fuchs R, Lehmann A, Green C. Ubiquitin-PCNA fusion as a mimic for mono-ubiquitinated PCNA in Schizosaccharomyces pombe. DNA Repair (Amst). 2010;9:777-84 pubmed publisher
    ..can reduce the UV sensitivity and increase translesion synthesis in mutants in which PCNA cannot be ubiquitinated (pcn1-K164R and rhp18), but not of the rad8 mutant in which PCNA can be mono-ubiquitinated but not poly-ubiquitinated...
  6. Dolan W, Le A, Schmidt H, Yuan J, Green M, Forsburg S. Fission yeast Hsk1 (Cdc7) kinase is required after replication initiation for induced mutagenesis and proper response to DNA alkylation damage. Genetics. 2010;185:39-53 pubmed publisher
    ..We conclude that Hsk1-Dfp1 kinase functions post-initiation to maintain replication fork stability, an activity potentially mediated by the C terminus of Dfp1. ..
  7. Dohke K, Miyazaki S, Tanaka K, Urano T, Grewal S, Murakami Y. Fission yeast chromatin assembly factor 1 assists in the replication-coupled maintenance of heterochromatin. Genes Cells. 2008;13:1027-43 pubmed publisher
    ..Taken together, we propose that spCAF1 functions in heterochromatin maintenance by recruiting dislocated Swi6 during replication to replicated heterochromatin at the replication fork. ..
  8. Frampton J, Irmisch A, Green C, Neiss A, Trickey M, Ulrich H, et al. Postreplication repair and PCNA modification in Schizosaccharomyces pombe. Mol Biol Cell. 2006;17:2976-85 pubmed
    ..We show that PCNA modification and cell cycle checkpoints represent two independent signals in response to DNA damage. Finally, we unexpectedly find that PCNA is ubiquitinated in response to DNA damage when cells are arrested in G2. ..
  9. Bermudez V, MacNeill S, Tappin I, Hurwitz J. The influence of the Cdc27 subunit on the properties of the Schizosaccharomyces pombe DNA polymerase delta. J Biol Chem. 2002;277:36853-62 pubmed
    ..In vivo, the introduction of these fused Cdc27 derivatives into cdc27Delta cells conferred viability. These data support the model in which Cdc27 plays an essential role in DNA replication by recruiting PCNA to the pol delta holoenzyme. ..

More Information


  1. Alleva J, Zuo S, Hurwitz J, Doetsch P. In vitro reconstitution of the Schizosaccharomyces pombe alternative excision repair pathway. Biochemistry. 2000;39:2659-66 pubmed
  2. Pietrobon V, Fréon K, Hardy J, Costes A, Iraqui I, Ochsenbein F, et al. The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1. PLoS Biol. 2014;12:e1001968 pubmed publisher
    ..We propose that CAF-1 plays a regulatory role during template switch by assembling chromatin on the D-loop and thereby impacting the resolution of the D-loop. ..
  3. Tanaka K, Yonekawa T, Kawasaki Y, Kai M, Furuya K, Iwasaki M, et al. Fission yeast Eso1p is required for establishing sister chromatid cohesion during S phase. Mol Cell Biol. 2000;20:3459-69 pubmed
    ..The C-terminal region acts to establish sister chromatid cohesion, and the N-terminal region presumably catalyzes translesion DNA synthesis when template DNA contains lesions that block regular DNA replication. ..
  4. Martin I, MacNeill S. Functional analysis of subcellular localization and protein-protein interaction sequences in the essential DNA ligase I protein of fission yeast. Nucleic Acids Res. 2004;32:632-42 pubmed
    ..Deletion of the PIP motif from the N-terminus of the nuclear form of Cdc17 fails to abolish Cdc17 function, indicating that PCNA binding by Cdc17 is not an absolute requirement for completion of S-phase. ..
  5. Zamir L, Zaretsky M, Fridman Y, Ner Gaon H, Rubin E, Aharoni A. Tight coevolution of proliferating cell nuclear antigen (PCNA)-partner interaction networks in fungi leads to interspecies network incompatibility. Proc Natl Acad Sci U S A. 2012;109:E406-14 pubmed publisher
    ..Our results indicate that the coevolution of PPI networks can form functional barriers between fungal species, and thus can promote and fix speciation...
  6. Guarino E, Shepherd M, Salguero I, Hua H, Deegan R, Kearsey S. Cdt1 proteolysis is promoted by dual PIP degrons and is modulated by PCNA ubiquitylation. Nucleic Acids Res. 2011;39:5978-90 pubmed publisher
    ..This provides a mechanism for fine-tuning the activity of the CRL4(Cdt2) pathway towards Cdt1, allowing Cdt1 proteolysis to be more efficient in S phase than after DNA damage...
  7. Álvarez V, Viñas L, Gallego Sánchez A, Andrés S, Sacristán M, Bueno A. Orderly progression through S-phase requires dynamic ubiquitylation and deubiquitylation of PCNA. Sci Rep. 2016;6:25513 pubmed publisher
    ..Our data suggest that the dynamic ubiquitylation and deubiquitylation of PCNA occurs during S-phase to ensure processive DNA replication. ..
  8. Ding L, Forsburg S. Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response. G3 (Bethesda). 2014;4:1373-84 pubmed publisher
  9. Groocock L, Prudden J, Perry J, Boddy M. The RecQ4 orthologue Hrq1 is critical for DNA interstrand cross-link repair and genome stability in fission yeast. Mol Cell Biol. 2012;32:276-87 pubmed publisher
    ..Thus, in hrq1? cells, lesions are channeled through the PRR pathway, yielding hyper-recombinant and mutator phenotypes; analogous defects may underlie the genetic instability and diseases associated with RecQ4 dysfunction...
  10. Salguero I, Guarino E, Shepherd M, Deegan T, Havens C, MacNeill S, et al. Ribonucleotide reductase activity is coupled to DNA synthesis via proliferating cell nuclear antigen. Curr Biol. 2012;22:720-6 pubmed publisher
    ..This mechanism thus provides a direct link between DNA synthesis and RNR regulation...
  11. Piard K, Baldacci G, Tratner I. Single point mutations located outside the inter-monomer domains abolish trimerization of Schizosaccharomyces pombe PCNA. Nucleic Acids Res. 1998;26:2598-605 pubmed
    ..for lethal mutations by testing for lack of complementation of a Schizosaccharomyces pombe strain disrupted for the pcn1 + gene. We thus identified eight lethal mutants out of the 50 cDNAs tested...
  12. Yamane K, Mizuguchi T, Cui B, Zofall M, Noma K, Grewal S. Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci. Mol Cell. 2011;41:56-66 pubmed publisher
    ..These analyses uncover a role for Asf1 in global histone deacetylation and suggest that HP1-associated histone chaperone promotes nucleosome occupancy to assemble repressive heterochromatin. ..
  13. Chen X, Zuo S, Kelman Z, O Donnell M, Hurwitz J, Goodman M. Fidelity of eucaryotic DNA polymerase delta holoenzyme from Schizosaccharomyces pombe. J Biol Chem. 2000;275:17677-82 pubmed
    ..A comparison of pol delta HE with E. coli pol IIIalpha HE (lacking the proofreading exonuclease subunit) showed that both holoenzymes exhibit similar error rates for each mispair. ..
  14. Ranatunga N, Forsburg S. Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4. G3 (Bethesda). 2016;6:3049-3063 pubmed publisher
    ..Surprisingly, we found that the deletion of rif1+ suppressed the MMS-sensitive phenotype without affecting temperature sensitivity. Together, these data suggest that mcm4-c106 destabilizes replisome structure. ..
  15. Arroyo M, Wang T. Mutant PCNA alleles are associated with cdc phenotypes and sensitivity to DNA damage in fission yeast. Mol Gen Genet. 1998;257:505-18 pubmed
    Twenty-eight site-directed mutations were introduced into the fission yeast gene (pcn1+) that encodes proliferating cell nuclear antigen (PCNA) and their in vivo effects analyzed in a strain with a null pcn1 background...
  16. Vejrup Hansen R, Mizuno K, Miyabe I, Fleck O, Holmberg C, Murray J, et al. Schizosaccharomyces pombe Mms1 channels repair of perturbed replication into Rhp51 independent homologous recombination. DNA Repair (Amst). 2011;10:283-95 pubmed publisher
    ..These observations suggest that Mms1 acts to channel repair of perturbed replication into a particular sub-pathway of homologous recombination. ..
  17. Arroyo M, Downey K, So A, Wang T. Schizosaccharomyces pombe proliferating cell nuclear antigen mutations affect DNA polymerase delta processivity. J Biol Chem. 1996;271:15971-80 pubmed
  18. Mastro T, Forsburg S. Increased meiotic crossovers and reduced genome stability in absence of Schizosaccharomyces pombe Rad16 (XPF). Genetics. 2014;198:1457-72 pubmed publisher
    ..Thus, the XPF/XPA module of the nucleotide excision repair pathway is incorporated into multiple aspects of genome maintenance even in the absence of external DNA damage. ..
  19. Pohler J, Otterlei M, Warbrick E. An in vivo analysis of the localisation and interactions of human p66 DNA polymerase delta subunit. BMC Mol Biol. 2005;6:17 pubmed
    ..The dominant negative effect upon growth resulting from expression of p66 sub-domains confirms that the p66-PCNA interaction is essential in vivo. ..
  20. Tallada V, Daga R, Palomeque C, Garzón A, Jimenez J. Genome-wide search of Schizosaccharomyces pombe genes causing overexpression-mediated cell cycle defects. Yeast. 2002;19:1139-51 pubmed
    ..The effect caused by some of them is described in more detail. ..
  21. Meister P, Taddei A, Ponti A, Baldacci G, Gasser S. Replication foci dynamics: replication patterns are modulated by S-phase checkpoint kinases in fission yeast. EMBO J. 2007;26:1315-26 pubmed
  22. Gray F, Pohler J, Warbrick E, MacNeill S. Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase delta subunit Cdc27. BMC Mol Biol. 2004;5:21 pubmed
    ..Despite being conserved across species, mutational analysis indicates the DPIM does not play an essential role in vivo, suggesting that interaction between the two polymerases is also non-essential. ..
  23. Chang D, Lu A. Interaction of checkpoint proteins Hus1/Rad1/Rad9 with DNA base excision repair enzyme MutY homolog in fission yeast, Schizosaccharomyces pombe. J Biol Chem. 2005;280:408-17 pubmed
    ..SpHus1 phosphorylation is substantially reduced in SpMYH Delta cells after hydrogen peroxide treatment. These data suggest that MYH may act as an adaptor to recruit checkpoint proteins to the DNA lesions. ..
  24. Daigaku Y, Etheridge T, Nakazawa Y, Nakayama M, Watson A, Miyabe I, et al. PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast. PLoS Genet. 2017;13:e1006789 pubmed publisher
  25. Coulon S, Ramasubramanyan S, Alies C, Philippin G, Lehmann A, Fuchs R. Rad8Rad5/Mms2-Ubc13 ubiquitin ligase complex controls translesion synthesis in fission yeast. EMBO J. 2010;29:2048-58 pubmed publisher
    ..This work highlights major differences in the control of lesion tolerance pathways between S. pombe and S. cerevisiae despite the homologous sets of PRR genes these organisms share. ..
  26. Meister P, Poidevin M, Francesconi S, Tratner I, Zarzov P, Baldacci G. Nuclear factories for signalling and repairing DNA double strand breaks in living fission yeast. Nucleic Acids Res. 2003;31:5064-73 pubmed
    ..Thus, in fission yeast, DSBs are detected by the DNA damage checkpoint and are repaired by homologous recombination at a few spatially confined subnuclear compartments where Rad22, Rad9 and PCNA concentrate independently. ..
  27. Chang D, Lu A. Functional interaction of MutY homolog with proliferating cell nuclear antigen in fission yeast, Schizosaccharomyces pombe. J Biol Chem. 2002;277:11853-8 pubmed
    ..Thus, the interaction between SpMYH and SpPCNA is important for SpMYH biological function in mutation avoidance. ..
  28. Grenon M, Tillit J, Piard K, Baldacci G, Francesconi S. The S/M checkpoint at 37 degrees C and the recovery of viability of the mutant poldeltats3 require the crb2+/rhp9+ gene in fission yeast. Mol Gen Genet. 1999;260:522-34 pubmed
    ..Finally, both the crb2 deletion and the crb2-1 allele induced a rapid death phenotype in the poldeltats3 background at both 30 degrees C and 37 degrees C. The rapid death phenotype was independent of the checkpoint functions. ..