Gene Symbol: nuc2
Description: anaphase-promoting complex subunit Apc3
Alias: SPAC1851.01, apc3, anaphase-promoting complex subunit Apc3
Species: fission yeast

Top Publications

  1. Kominami K, Seth Smith H, Toda T. Apc10 and Ste9/Srw1, two regulators of the APC-cyclosome, as well as the CDK inhibitor Rum1 are required for G1 cell-cycle arrest in fission yeast. EMBO J. 1998;17:5388-99 pubmed
    ..The results suggest that dual downregulation of CDK, one via the APC and the other via the CDK inhibitor, is a universal mechanism that is used to arrest cell cycle progression at G1. ..
  2. Hirano T, Hiraoka Y, Yanagida M. A temperature-sensitive mutation of the Schizosaccharomyces pombe gene nuc2+ that encodes a nuclear scaffold-like protein blocks spindle elongation in mitotic anaphase. J Cell Biol. 1988;106:1171-83 pubmed
    A temperature-sensitive mutant nuc2-663 of the fission yeast Schizosaccharomyces pombe specifically blocks mitotic spindle elongation at restrictive temperature so that nuclei in arrested cells contain a short uniform spindle (..
  3. Kumada K, Su S, Yanagida M, Toda T. Fission yeast TPR-family protein nuc2 is required for G1-arrest upon nitrogen starvation and is an inhibitor of septum formation. J Cell Sci. 1995;108 ( Pt 3):895-905 pubmed
    Fission yeast nuc2+ gene encodes a protein of a tetratricopeptide repeat (TPR) family which is conserved throughout evolution. We previously showed that nuc2 is required for exit from the mitotic metaphase...
  4. Kimata Y, Trickey M, Izawa D, Gannon J, Yamamoto M, Yamano H. A mutual inhibition between APC/C and its substrate Mes1 required for meiotic progression in fission yeast. Dev Cell. 2008;14:446-54 pubmed publisher
    ..These results demonstrate that the fine-tuning of the APC/C activity, by a substrate that is also an inhibitor, is required for the precise coordination and transition through meiosis. ..
  5. He X, Patterson T, Sazer S. The Schizosaccharomyces pombe spindle checkpoint protein mad2p blocks anaphase and genetically interacts with the anaphase-promoting complex. Proc Natl Acad Sci U S A. 1997;94:7965-70 pubmed
  6. Berry L, Feoktistova A, Wright M, Gould K. The schizosaccharomyces pombe dim1(+) gene interacts with the anaphase-promoting complex or cyclosome (APC/C) component lid1(+) and is required for APC/C function. Mol Cell Biol. 1999;19:2535-46 pubmed
    ..These data suggest that at least one role of dim1p is to maintain or establish the steady-state level of the APC/C. ..
  7. Chang L, Morrell J, Feoktistova A, Gould K. Study of cyclin proteolysis in anaphase-promoting complex (APC) mutant cells reveals the requirement for APC function in the final steps of the fission yeast septation initiation network. Mol Cell Biol. 2001;21:6681-94 pubmed
    ..Indeed, overproduction of nondestructible Cdc13p prevents septation in APC cut mutants and the normal reorganization of septation initiation network components during anaphase. ..
  8. Samejima I, Yanagida M. Bypassing anaphase by fission yeast cut9 mutation: requirement of cut9+ to initiate anaphase. J Cell Biol. 1994;127:1655-70 pubmed
    ..It is essential for viability, and the mutation sites reside in the TPR. The three genes, namely, nuc2+, scn1+, and scn2+, genetically interact with cut9+...
  9. Ohi M, Feoktistova A, Ren L, Yip C, Cheng Y, Chen J, et al. Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1. Mol Cell. 2007;28:871-85 pubmed
    ..Comparison of the APC/C with another multiprotein E3 ligase, the SCF complex, uncovers remarkable structural similarities. ..

More Information


  1. Yamada H, Kumada K, Yanagida M. Distinct subunit functions and cell cycle regulated phosphorylation of 20S APC/cyclosome required for anaphase in fission yeast. J Cell Sci. 1997;110 ( Pt 15):1793-804 pubmed
    We show here that the fission yeast gene products Cut9 and Nuc2 are the subunits of the 20S complex, the putative APC (anaphase promoting complex)/cyclosome which contains ubiquitin ligase activity required for cyclin and Cut2 destruction...
  2. Yamano H, Tsurumi C, Gannon J, Hunt T. The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor. EMBO J. 1998;17:5670-8 pubmed
    ..These data suggest the existence of a D-box receptor protein that recognizes D-boxes prior to ubiquitination. ..
  3. Yamashita Y, Nakaseko Y, Kumada K, Nakagawa T, Yanagida M. Fission yeast APC/cyclosome subunits, Cut20/Apc4 and Cut23/Apc8, in regulating metaphase-anaphase progression and cellular stress responses. Genes Cells. 1999;4:445-63 pubmed
    ..In fission yeast, however, only five (Cut4, Cut9, Nuc2, Apc10, Hcn1) have been identified...
  4. Yanagida M. Gene products required for chromosome separation. J Cell Sci Suppl. 1989;12:213-29 pubmed
    ..The roles of four representative genes, namely nda3+, nuc2+, top2+ and dis2+, encoding beta-tubulin, a nuclear scaffold-like protein, DNA topoisomerase II and type-1 protein ..
  5. Sczaniecka M, Feoktistova A, May K, Chen J, Blyth J, Gould K, et al. The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C). J Biol Chem. 2008;283:23039-47 pubmed publisher
    ..Our findings demonstrate that this Mad3 KEN box is a critical mediator of Cdc20-APC/C inhibition, without which neither Mad3 nor Mad2 can associate with the APC/C or inhibit anaphase onset. ..
  6. Yoon H, Feoktistova A, Chen J, Jennings J, Link A, Gould K. Role of Hcn1 and its phosphorylation in fission yeast anaphase-promoting complex/cyclosome function. J Biol Chem. 2006;281:32284-93 pubmed
    ..Phosphorylation of Hcn1 occurs at a single Cdk1 site in vitro and in vivo. Mutation of this site to alanine, but not aspartic acid, compromises APC/C function and leads to a specific defect in the completion of cell division. ..
  7. Matsumura T, Yuasa T, Hayashi T, Obara T, Kimata Y, Yanagida M. A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids. Genes Cells. 2003;8:341-55 pubmed
    ..In addition, the manner of the inhibition by high copy securin and separase plasmids were quite different each other and did not result in the mitotic block. ..
  8. Hirano T, Kinoshita N, Morikawa K, Yanagida M. Snap helix with knob and hole: essential repeats in S. pombe nuclear protein nuc2+. Cell. 1990;60:319-28 pubmed
    The S. pombe nuc2+ gene is required for mitotic chromosome disjunction. Its mutation arrests mitosis at the metaphase. The gene product is present in the nuclear scaffold-like fraction...
  9. Grishchuk E, Frolov D, Savchenko G. [Overexpression of the apc10+ gene in the fission yeast Schizosaccharomyces pombe can suppress temperature sensitivity of the nuc2-663 mutant,but not its sterility]. Mol Biol (Mosk). 2000;34:809-15 pubmed
  10. Grishchuk E, Howe J, McIntosh J. A screen for genes involved in the anaphase proteolytic pathway identifies tsm1(+), a novel Schizosaccharomyces pombe gene important for microtubule integrity. Genetics. 1998;149:1251-64 pubmed
    The growth of several mitotic mutants of Schizosaccharomyces pombe, including nuc2-663, is inhibited by the protease inhibitor N-Tosyl-L-Phenylalanine Chloromethyl Ketone (TPCK)...
  11. Ishii K, Kumada K, Toda T, Yanagida M. Requirement for PP1 phosphatase and 20S cyclosome/APC for the onset of anaphase is lessened by the dosage increase of a novel gene sds23+. EMBO J. 1996;15:6629-40 pubmed
    ..These results demonstrate functions other than promoting anaphase for the components of the 20S cyclosome/APC and also a close functional relationship of Sds23 with PP1 and 20S cyclosome/APC. ..
  12. Yamashita Y, Nakaseko Y, Samejima I, Kumada K, Yamada H, Michaelson D, et al. 20S cyclosome complex formation and proteolytic activity inhibited by the cAMP/PKA pathway. Nature. 1996;384:276-9 pubmed
    ..Suppression correlates with the amount of 20S complex, indicating that cyclosome formation and activation is inhibited by the cAMP/PKA pathway. ..
  13. Kimata Y, Kitamura K, Fenner N, Yamano H. Mes1 controls the meiosis I to meiosis II transition by distinctly regulating the anaphase-promoting complex/cyclosome coactivators Fzr1/Mfr1 and Slp1 in fission yeast. Mol Biol Cell. 2011;22:1486-94 pubmed publisher
    ..Thus Mes1 interacts with two coactivators in a different way to control the activity of the APC/C required for the meiosis I/meiosis II transition...
  14. Chew T, Balasubramanian M. Nuc2p, a subunit of the anaphase-promoting complex, inhibits septation initiation network following cytokinesis in fission yeast. PLoS Genet. 2008;4:e17 pubmed publisher
    ..We propose that Nuc2p, by inhibiting the SIN after cell division, prevents further deleterious cytokinetic events, thereby contributing to genome stability. ..
  15. Kimata Y, Matsuyama A, Nagao K, Furuya K, Obuse C, Yoshida M, et al. Diminishing HDACs by drugs or mutations promotes normal or abnormal sister chromatid separation by affecting APC/C and adherin. J Cell Sci. 2008;121:1107-18 pubmed publisher
    ..HATs and HDACs might directly target adherin and APC/C to ensure proper chromosome segregation, and anti-tumour effects of HDAC inhibitors could be attributed to this deregulation. ..