mad3

Summary

Gene Symbol: mad3
Description: mitotic spindle checkpoint protein Mad3
Alias: SPCC895.02, mitotic spindle checkpoint protein Mad3
Species: fission yeast

Top Publications

  1. Sczaniecka M, Feoktistova A, May K, Chen J, Blyth J, Gould K, et al. The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C). J Biol Chem. 2008;283:23039-47 pubmed publisher
    ..In the fission yeast, Schizosaccharomyces pombe, we find that the Mad3 and Mad2 spindle checkpoint proteins interact stably with the APC/C in mitosis...
  2. Millband D, Hardwick K. Fission yeast Mad3p is required for Mad2p to inhibit the anaphase-promoting complex and localizes to kinetochores in a Bub1p-, Bub3p-, and Mph1p-dependent manner. Mol Cell Biol. 2002;22:2728-42 pubmed
    ..Here we present the fission yeast homologue of Mad3p. Cells devoid of mad3(+) are unable to arrest their cell cycle in the presence of microtubule defects...
  3. Meadows J, Shepperd L, Vanoosthuyse V, Lancaster T, Sochaj A, Buttrick G, et al. Spindle checkpoint silencing requires association of PP1 to both Spc7 and kinesin-8 motors. Dev Cell. 2011;20:739-50 pubmed publisher
    ..These data demonstrate that at least two distinct pools of PP1, one kinetochore associated and the other motor associated, are needed to silence the spindle checkpoint. ..
  4. Tange Y, Niwa O. Novel mad2 alleles isolated in a Schizosaccharomyces pombe gamma-tubulin mutant are defective in metaphase arrest activity, but remain functional for chromosome stability in unperturbed mitosis. Genetics. 2007;175:1571-84 pubmed
    ..Immunoprecipitation studies demonstrated that GFP-fused mutant Mad2 proteins formed a Mad1-containing complex with altered stability compared to that formed with wild-type Mad2, providing clues to the novel mad2 mutant phenotype. ..
  5. Ohi M, Feoktistova A, Ren L, Yip C, Cheng Y, Chen J, et al. Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1. Mol Cell. 2007;28:871-85 pubmed
    ..Comparison of the APC/C with another multiprotein E3 ligase, the SCF complex, uncovers remarkable structural similarities. ..
  6. Kadura S, He X, Vanoosthuyse V, Hardwick K, Sazer S. The A78V mutation in the Mad3-like domain of Schizosaccharomyces pombe Bub1p perturbs nuclear accumulation and kinetochore targeting of Bub1p, Bub3p, and Mad3p and spindle assembly checkpoint function. Mol Biol Cell. 2005;16:385-95 pubmed
    ..interest because it produces a wild-type amount of protein that is mutated in the conserved but uncharacterized Mad3-like region of Bub1p...
  7. Zich J, May K, Paraskevopoulos K, Sen O, Syred H, van der Sar S, et al. Mps1Mph1 Kinase Phosphorylates Mad3 to Inhibit Cdc20Slp1-APC/C and Maintain Spindle Checkpoint Arrests. PLoS Genet. 2016;12:e1005834 pubmed publisher
    ..b>Mad3/BubR1 is a key component of the mitotic checkpoint complex (MCC) which binds and inhibits the APC/C early in ..
  8. Zich J, Sochaj A, Syred H, Milne L, Cook A, Ohkura H, et al. Kinase activity of fission yeast Mph1 is required for Mad2 and Mad3 to stably bind the anaphase promoting complex. Curr Biol. 2012;22:296-301 pubmed publisher
    ..Mad2 and Mad3 (BubR1) then bind and inhibit Cdc20 to form the mitotic checkpoint complex (MCC), which binds and inhibits the ..
  9. Minoda A, Saitoh S, Takahashi K, Toda T. BAF53/Arp4 homolog Alp5 in fission yeast is required for histone H4 acetylation, kinetochore-spindle attachment, and gene silencing at centromere. Mol Biol Cell. 2005;16:316-27 pubmed
    ..This result shows that Alp5 is essential for histone H4 acetylation, and its crucial role lies in the establishment of bipolar attachment of the kinetochore to the spindle and transcriptional silencing at the centromere. ..

More Information

Publications17

  1. Shepperd L, Meadows J, Sochaj A, Lancaster T, Zou J, Buttrick G, et al. Phosphodependent recruitment of Bub1 and Bub3 to Spc7/KNL1 by Mph1 kinase maintains the spindle checkpoint. Curr Biol. 2012;22:891-9 pubmed publisher
    ..Components of the checkpoint include Mad1, Mad2, Mad3 (BubR1), Bub3, and the kinases Bub1, Mph1 (Mps1), and Aurora B...
  2. Choi S, Péli Gulli M, Mcleod I, Sarkeshik A, Yates J, Simanis V, et al. Phosphorylation state defines discrete roles for monopolin in chromosome attachment and spindle elongation. Curr Biol. 2009;19:985-95 pubmed publisher
    ..Together, these findings illustrate how the sequential phosphorylation and dephosphorylation of monopolin helps ensure the orderly execution of discrete steps in mitosis. ..
  3. Sewart K, Hauf S. Different Functionality of Cdc20 Binding Sites within the Mitotic Checkpoint Complex. Curr Biol. 2017;27:1213-1220 pubmed publisher
    ..MCC binds two Cdc20 molecules, one (Cdc20M) through well-characterized, cooperative binding to Mad2 and Mad3/BubR1 (forming the "core MCC") and the other one (Cdc20A) through additional binding sequences in Mad3/..
  4. Heinrich S, Windecker H, Hustedt N, Hauf S. Mph1 kinetochore localization is crucial and upstream in the hierarchy of spindle assembly checkpoint protein recruitment to kinetochores. J Cell Sci. 2012;125:4720-7 pubmed publisher
    ..to kinetochores and identify a three-layered hierarchy with Ark1 and Mph1 on top, Bub1 and Bub3 in the middle, and Mad3 as well as the Mad1-Mad2 complex at the lower end of the hierarchy...
  5. Asakawa K, Toya M, Sato M, Kanai M, Kume K, Goshima T, et al. Mal3, the fission yeast EB1 homologue, cooperates with Bub1 spindle checkpoint to prevent monopolar attachment. EMBO Rep. 2005;6:1194-200 pubmed
    ..Double mutants between mal3 and a subset of checkpoint mutants, including bub1, bub3, mad3 and mph1, but not mad1 or mad2, show massive chromosome mis-segregation defects...
  6. Ito D, Saito Y, Matsumoto T. Centromere-tethered Mps1 pombe homolog (Mph1) kinase is a sufficient marker for recruitment of the spindle checkpoint protein Bub1, but not Mad1. Proc Natl Acad Sci U S A. 2012;109:209-14 pubmed publisher
    ..We propose that Mph1 is a sufficient marker for recruitment of Bub1. Mad1, in contrast, requires an additional condition/component for stable association with kinetochores. ..
  7. Hou H, Zhou Z, Wang Y, Wang J, Kallgren S, Kurchuk T, et al. Csi1 links centromeres to the nuclear envelope for centromere clustering. J Cell Biol. 2012;199:735-44 pubmed publisher
    ..These studies thus define a molecular link between the centromere and nuclear envelope that is responsible for centromere clustering...
  8. Petersen J, Hagan I. S. pombe aurora kinase/survivin is required for chromosome condensation and the spindle checkpoint attachment response. Curr Biol. 2003;13:590-7 pubmed
    ..Ark1/survivin function was not required for the association of Bub1 or Mad3 with the kinetochores...