klp5

Summary

Gene Symbol: klp5
Description: kinesin-like protein Klp5
Alias: sot1, kinesin-like protein Klp5
Species: fission yeast

Top Publications

  1. Grissom P, Fiedler T, Grishchuk E, Nicastro D, West R, McIntosh J. Kinesin-8 from fission yeast: a heterodimeric, plus-end-directed motor that can couple microtubule depolymerization to cargo movement. Mol Biol Cell. 2009;20:963-72 pubmed publisher
    Fission yeast expresses two kinesin-8s, previously identified and characterized as products of the klp5(+) and klp6(+) genes...
  2. West R, Malmstrom T, Troxell C, McIntosh J. Two related kinesins, klp5+ and klp6+, foster microtubule disassembly and are required for meiosis in fission yeast. Mol Biol Cell. 2001;12:3919-32 pubmed
    ..We have characterized two related kinesins in fission yeast, klp5+ and klp6+,, that are amino-terminal motors of the KIP3 subfamily...
  3. West R, Malmstrom T, McIntosh J. Kinesins klp5(+) and klp6(+) are required for normal chromosome movement in mitosis. J Cell Sci. 2002;115:931-40 pubmed
    ..Here we demonstrate that two related fission yeast kinesins, klp5(+) and klp6(+), are required for normal chromosome segregation in mitosis...
  4. Garcia M, Koonrugsa N, Toda T. Spindle-kinetochore attachment requires the combined action of Kin I-like Klp5/6 and Alp14/Dis1-MAPs in fission yeast. EMBO J. 2002;21:6015-24 pubmed
    Fission yeast Klp5 and Klp6 belong to the microtubule-destabilizing Kin I family...
  5. Meadows J, Shepperd L, Vanoosthuyse V, Lancaster T, Sochaj A, Buttrick G, et al. Spindle checkpoint silencing requires association of PP1 to both Spc7 and kinesin-8 motors. Dev Cell. 2011;20:739-50 pubmed publisher
    ..that association of type 1 phosphatase (PP1(Dis2)) with both the N terminus of Spc7 and the nonmotor domains of the Klp5-Klp6 (kinesin-8) complex is necessary to counteract Aurora B kinase to efficiently silence the spindle checkpoint...
  6. Sato M, Vardy L, Angel Garcia M, Koonrugsa N, Toda T. Interdependency of fission yeast Alp14/TOG and coiled coil protein Alp7 in microtubule localization and bipolar spindle formation. Mol Biol Cell. 2004;15:1609-22 pubmed
    ..Our results shed light on an interdependent relationship between Alp14/TOG and Alp7. We propose a two-step model that accounts for the recruitment of Alp7 and Alp14 to the SPB and microtubules. ..
  7. Sanchez Perez I, Renwick S, Crawley K, Karig I, Buck V, Meadows J, et al. The DASH complex and Klp5/Klp6 kinesin coordinate bipolar chromosome attachment in fission yeast. EMBO J. 2005;24:2931-43 pubmed
    ..We find that the mitotic centromere-associated Klp5/Klp6 kinesin complex is essential in cells lacking components of the DASH complex...
  8. Unsworth A, Masuda H, Dhut S, Toda T. Fission yeast kinesin-8 Klp5 and Klp6 are interdependent for mitotic nuclear retention and required for proper microtubule dynamics. Mol Biol Cell. 2008;19:5104-15 pubmed publisher
    Fission yeast has two kinesin-8s, Klp5 and Klp6, which associate to form a heterocomplex. Here, we show that Klp5 and Klp6 are mutually dependent on each other for nuclear mitotic localization...
  9. Syrovatkina V, Fu C, Tran P. Antagonistic spindle motors and MAPs regulate metaphase spindle length and chromosome segregation. Curr Biol. 2013;23:2423-9 pubmed publisher
    ..cut7p and MT bundler ase1p contribute to outward-pushing forces and that the spindle kinetochore proteins kinesin-8 klp5/6p and dam1p contribute to inward-pulling forces...

More Information

Publications21

  1. Garcia M, Koonrugsa N, Toda T. Two kinesin-like Kin I family proteins in fission yeast regulate the establishment of metaphase and the onset of anaphase A. Curr Biol. 2002;12:610-21 pubmed
    ..We have characterized mitotic roles of two Kin I homologs, Klp5 and Klp6, in fission yeast. Klp5 and Klp6 colocalize to the mitotic kinetochores and the spindle midzone...
  2. West R, McIntosh J. Novel interactions of fission yeast kinesin 8 revealed through in vivo expression of truncation alleles. Cell Motil Cytoskeleton. 2008;65:626-40 pubmed publisher
    Fission yeast expresses two kinesin 8s, klp5+ and klp6+, which are important for diverse cellular functions: mitosis, meiosis, and the maintenance of normal cell morphology...
  3. Berger K, Sogo L, Yaffe M. Mdm12p, a component required for mitochondrial inheritance that is conserved between budding and fission yeast. J Cell Biol. 1997;136:545-53 pubmed
    ..cerevisiae and the first such mitochondrial component that is conserved between two different species. ..
  4. Tischer C, Brunner D, Dogterom M. Force- and kinesin-8-dependent effects in the spatial regulation of fission yeast microtubule dynamics. Mol Syst Biol. 2009;5:250 pubmed publisher
    ..evidence for an MT length-dependent increase in the catastrophe frequency that is mediated by kinesin-8 proteins (Klp5/6)...
  5. Chen J, Lu L, Ohi M, Creamer K, English C, Partridge J, et al. Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation. J Cell Biol. 2011;195:583-93 pubmed publisher
    ..Our findings establish new roles for Cdk1 and the Nsk1-Dlc1 complex in regulating the k-MT interface and chromosome segregation. ..
  6. Buttrick G, Lancaster T, Meadows J, Millar J. Plo1 phosphorylates Dam1 to promote chromosome bi-orientation in fission yeast. J Cell Sci. 2012;125:1645-51 pubmed publisher
    ..We show that Dam1 phosphorylation by Plo1 is dispensable for DASH assembly and chromosome retrieval but instead aids tension-dependent chromosome bi-orientation. ..
  7. Wälde S, King M. The KASH protein Kms2 coordinates mitotic remodeling of the spindle pole body. J Cell Sci. 2014;127:3625-40 pubmed publisher
    ..Plo1 activity supports mitotic SPB remodeling by driving a burst of incorporation of Cut12 and Pcp1. Thus, a fission yeast SUN-KASH complex plays an important role in supporting the remodeling of the SPB at mitotic entry. ..
  8. Tang N, Toda T. Alp7/TACC recruits kinesin-8-PP1 to the Ndc80 kinetochore protein for timely mitotic progression and chromosome movement. J Cell Sci. 2015;128:354-63 pubmed publisher
    ..study, we show that the fission yeast Alp7/TACC protein recruits a protein complex consisting of the kinesin-8 (Klp5-Klp6) and protein phosphatase 1 (PP1) to the kinetochore upon kinetochore-microtubule attachment...
  9. Gergely Z, Crapo A, Hough L, McIntosh J, Betterton M. Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast. Mol Biol Cell. 2016;27:3490-3514 pubmed
    ..role of kinesin-8 proteins in mitosis, we studied the effects of deletion of the fission yeast kinesin-8 proteins Klp5 and Klp6 on chromosome movements and spindle length dynamics...
  10. Tange Y, Fujita A, Toda T, Niwa O. Functional dissection of the gamma-tubulin complex by suppressor analysis of gtb1 and alp4 mutations in Schizosaccharomyces pombe. Genetics. 2004;167:1095-107 pubmed
    ..In addition, genetic data indicated intimate functional connections of gamma-tubulin with several kinesin-like proteins. ..
  11. Li Y, Chang E. Schizosaccharomyces pombe Ras1 effector, Scd1, interacts with Klp5 and Klp6 kinesins to mediate cytokinesis. Genetics. 2003;165:477-88 pubmed
    ..In a screen for scd1 interacting genes, we isolated klp5 and klp6, which encode presumptive kinesins...
  12. Lucena R, Dephoure N, Gygi S, Kellogg D, Tallada V, Daga R, et al. Nucleocytoplasmic transport in the midzone membrane domain controls yeast mitotic spindle disassembly. J Cell Biol. 2015;209:387-402 pubmed publisher
    ..Our findings illustrate how a separate membrane compartment supports spindle disassembly in the closed mitosis of fission yeast. ..