Gene Symbol: dis2
Description: serine/threonine protein phosphatase PP1
Alias: bws1, sds1, serine/threonine protein phosphatase PP1
Species: fission yeast
Products:     dis2

Top Publications

  1. Meadows J, Shepperd L, Vanoosthuyse V, Lancaster T, Sochaj A, Buttrick G, et al. Spindle checkpoint silencing requires association of PP1 to both Spc7 and kinesin-8 motors. Dev Cell. 2011;20:739-50 pubmed publisher
    ..We demonstrate that association of type 1 phosphatase (PP1(Dis2)) with both the N terminus of Spc7 and the nonmotor domains of the Klp5-Klp6 (kinesin-8) complex is necessary to ..
  2. Alvarez Tabarés I, Grallert A, Ortiz J, Hagan I. Schizosaccharomyces pombe protein phosphatase 1 in mitosis, endocytosis and a partnership with Wsh3/Tea4 to control polarised growth. J Cell Sci. 2007;120:3589-601 pubmed
    ..The Schizosaccharomyces pombe genome encodes two highly related catalytic subunits, Dis2 and Sds21...
  3. Yamano H, Ishii K, Yanagida M. Phosphorylation of dis2 protein phosphatase at the C-terminal cdc2 consensus and its potential role in cell cycle regulation. EMBO J. 1994;13:5310-8 pubmed
    We show that the fission yeast dis2 protein phosphatase, which is highly similar to mammalian type 1 phosphatase, is a phosphoprotein containing phosphoserine (phospho-S) and threonine (phospho-T)...
  4. Hiraga S, Alvino G, Chang F, Lian H, Sridhar A, Kubota T, et al. Rif1 controls DNA replication by directing Protein Phosphatase 1 to reverse Cdc7-mediated phosphorylation of the MCM complex. Genes Dev. 2014;28:372-83 pubmed publisher
    ..Since PP1 interaction sites are evolutionarily conserved within the Rif1 sequence, it is likely that replication control by Rif1 through PP1 is a conserved mechanism. ..
  5. Vanoosthuyse V, Hardwick K. A novel protein phosphatase 1-dependent spindle checkpoint silencing mechanism. Curr Biol. 2009;19:1176-81 pubmed publisher
    ..silencing and cyclin B degradation require the kinetochore-localized isoform of protein phosphatase 1 (PP1(Dis2))...
  6. Ohkura H, Kinoshita N, Miyatani S, Toda T, Yanagida M. The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases. Cell. 1989;57:997-1007 pubmed
    ..two independent genomic DNAs that complemented both the cold-sensitive and caffeine-hypersensitive phenotype of dis2-11...
  7. Ohkura H, Adachi Y, Kinoshita N, Niwa O, Toda T, Yanagida M. Cold-sensitive and caffeine-supersensitive mutants of the Schizosaccharomyces pombe dis genes implicated in sister chromatid separation during mitosis. EMBO J. 1988;7:1465-73 pubmed
    ..dis mutants that block mitotic chromosome separation (nine mapped in the dis1 gene and one each in the dis2 and dis3 genes)...
  8. Grallert A, Chan K, Alonso Nuñez M, Madrid M, Biswas A, Alvarez Tabarés I, et al. Removal of centrosomal PP1 by NIMA kinase unlocks the MPF feedback loop to promote mitotic commitment in S. pombe. Curr Biol. 2013;23:213-22 pubmed publisher
    ..Late G2 phase phosphorylation between the two elements by MPF and the NIMA kinase Fin1 blocked PP1(Dis2) recruitment, thereby promoting recruitment of Polo to Cut12 and the SPB and elevating global Polo kinase activity ..
  9. Buttrick G, Lancaster T, Meadows J, Millar J. Plo1 phosphorylates Dam1 to promote chromosome bi-orientation in fission yeast. J Cell Sci. 2012;125:1645-51 pubmed publisher
    ..We show that Dam1 phosphorylation by Plo1 is dispensable for DASH assembly and chromosome retrieval but instead aids tension-dependent chromosome bi-orientation. ..

More Information


  1. Dav A, Cooley C, Garg M, Bianchi A. Protein phosphatase 1 recruitment by Rif1 regulates DNA replication origin firing by counteracting DDK activity. Cell Rep. 2014;7:53-61 pubmed publisher
    ..These findings elucidate the mechanism of action of Rif1 in the control of DNA replication and demonstrate a role of PP1 phosphatases in the regulation of origin firing...
  2. Roguev A, Shevchenko A, Schaft D, Thomas H, Stewart A, Shevchenko A. A comparative analysis of an orthologous proteomic environment in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. Mol Cell Proteomics. 2004;3:125-32 pubmed
    ..We speculate that conservation of the core components of protein assemblies and variability of hyperlinks represents a general principle in the molecular organization of eukaryotic proteomes. ..
  3. Tange Y, Hirata A, Niwa O. An evolutionarily conserved fission yeast protein, Ned1, implicated in normal nuclear morphology and chromosome stability, interacts with Dis3, Pim1/RCC1 and an essential nucleoporin. J Cell Sci. 2002;115:4375-85 pubmed
    ..The ned1(+) gene belongs to an evolutionarily conserved gene family, which includes the mouse Lpin genes, one of whose mutations is responsible for lipodystrophy. ..
  4. Tang N, Toda T. Alp7/TACC recruits kinesin-8-PP1 to the Ndc80 kinetochore protein for timely mitotic progression and chromosome movement. J Cell Sci. 2015;128:354-63 pubmed publisher
    ..We propose that the recruitment of kinesin-8-PP1 to the kinetochore through Alp7/TACC interaction plays a crucial role in regulation of timely mitotic progression and chromosome movement during anaphase A. ..
  5. den Elzen N, O Connell M. Recovery from DNA damage checkpoint arrest by PP1-mediated inhibition of Chk1. EMBO J. 2004;23:908-18 pubmed
    ..We show that overexpression of the type I protein phosphatase Dis2 sensitises S. pombe cells to DNA damage, causing aberrant mitoses...
  6. Yamada H, Kumada K, Yanagida M. Distinct subunit functions and cell cycle regulated phosphorylation of 20S APC/cyclosome required for anaphase in fission yeast. J Cell Sci. 1997;110 ( Pt 15):1793-804 pubmed
    ..In contrast, PP1 (Dis2) phosphatase mutation shows the reverse effect on the phenotype of cut9...
  7. Ohkura H, Yanagida M. S. pombe gene sds22+ essential for a midmitotic transition encodes a leucine-rich repeat protein that positively modulates protein phosphatase-1. Cell. 1991;64:149-57 pubmed
    The fission yeast dis2+ gene encodes one of the two type 1 protein phosphatases (PP1) in this organism. Its semidominant mutant dis2-11 is defective in mitosis...
  8. Kinoshita N, Ohkura H, Yanagida M. Distinct, essential roles of type 1 and 2A protein phosphatases in the control of the fission yeast cell division cycle. Cell. 1990;63:405-15 pubmed
    ..Two previously identified PP1 genes (dis2+ and sds21+) and two PP2A genes (ppa1+ and ppa2+), highly homologous to mammalian PP2A, have been isolated from ..
  9. Kinoshita N, Goebl M, Yanagida M. The fission yeast dis3+ gene encodes a 110-kDa essential protein implicated in mitotic control. Mol Cell Biol. 1991;11:5839-47 pubmed
    ..Its phenotype is similar to that of dis2-11, a mutant with a mutation in the type 1 protein phosphatase gene. We cloned the dis3+ gene by transformation...
  10. Xu X, Nakazawa N, Yanagida M. Condensin HEAT subunits required for DNA repair, kinetochore/centromere function and ploidy maintenance in fission yeast. PLoS ONE. 2015;10:e0119347 pubmed publisher
    ..Five of these mutations reside in helix B of HEAT repeats. Two non-SMC condensin subunits, Cnd1 and Cnd3, are thus implicated in ploidy maintenance. ..
  11. Grallert A, Boke E, Hagting A, Hodgson B, Connolly Y, Griffiths J, et al. A PP1-PP2A phosphatase relay controls mitotic progression. Nature. 2015;517:94-98 pubmed publisher
    ..The staged recruitment of PP1 (the Dis2 isoform) to the regulatory subunits of the PP2A-B55 and PP2A-B56 (B55 also known as Pab1; B56 also known as Par1) ..
  12. Maruyama T, Nakamura T, Hayashi T, Yanagida M. Histone H2B mutations in inner region affect ubiquitination, centromere function, silencing and chromosome segregation. EMBO J. 2006;25:2420-31 pubmed
    ..Histone H2B thus plays an important role in centromere/kinetochore formation. ..
  13. Stone E, Yamano H, Kinoshita N, Yanagida M. Mitotic regulation of protein phosphatases by the fission yeast sds22 protein. Curr Biol. 1993;3:13-26 pubmed
    ..We have previously reported that the dis2(+) and sds2(+) genes of fission yeast encode redundant catalytic subunits of a type 1-like protein phosphatase...
  14. Tatebe H, Yanagida M. Cut8, essential for anaphase, controls localization of 26S proteasome, facilitating destruction of cyclin and Cut2. Curr Biol. 2000;10:1329-38 pubmed
    ..Cut8 is a novel evolutionarily conserved heat-inducible regulator. It facilitates anaphase-promoting proteolysis by recruiting 26S proteasome to a functionally efficient nuclear location. ..
  15. Kondoh H, Yuasa T, Yanagida M. Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint. Genes Cells. 2000;5:525-41 pubmed
    ..Mis3 is essential for ribosome biogenesis, supports S phase checkpoint, and is needed for the coupling between growth and cell cycle. Whether Mis3 interacts solely with ribosomal precursor RNA remains to be determined. ..
  16. Hachet O, Berthelot Grosjean M, Kokkoris K, Vincenzetti V, Moosbrugger J, Martin S. A phosphorylation cycle shapes gradients of the DYRK family kinase Pom1 at the plasma membrane. Cell. 2011;145:1116-28 pubmed publisher
    ..Tea4 triggers Pom1 plasma membrane association by promoting its dephosphorylation through the protein phosphatase 1 Dis2. We propose that local dephosphorylation induces Pom1 membrane association and nucleates a gradient shaped by the ..
  17. Aoki K, Nakaseko Y, Kinoshita K, Goshima G, Yanagida M. CDC2 phosphorylation of the fission yeast dis1 ensures accurate chromosome segregation. Curr Biol. 2006;16:1627-35 pubmed
    ..Cdc2 thus directly phosphorylates Dis1, and this phosphorylation regulates Dis1 localization in both metaphase and anaphase and ensures high-fidelity segregation. ..
  18. Kokkoris K, Gallo Castro D, Martin S. The Tea4-PP1 landmark promotes local growth by dual Cdc42 GEF recruitment and GAP exclusion. J Cell Sci. 2014;127:2005-16 pubmed publisher
    ..This activity requires that Tea4 binds the type I phosphatase (PP1) catalytic subunit Dis2 or Sds21, and ectopic targeting of either catalytic subunit is similarly instructive for growth...
  19. Chen J, Lu L, Ohi M, Creamer K, English C, Partridge J, et al. Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation. J Cell Biol. 2011;195:583-93 pubmed publisher
    ..Our findings establish new roles for Cdk1 and the Nsk1-Dlc1 complex in regulating the k-MT interface and chromosome segregation. ..
  20. Kawashima S, Takemoto A, Nurse P, Kapoor T. A chemical biology strategy to analyze rheostat-like protein kinase-dependent regulation. Chem Biol. 2013;20:262-71 pubmed publisher
    ..Our strategy is general and can be used to examine the functions of other molecular rheostats. ..
  21. Booher R, Beach D. Interaction between cdc13+ and cdc2+ in the control of mitosis in fission yeast; dissociation of the G1 and G2 roles of the cdc2+ protein kinase. EMBO J. 1987;6:3441-7 pubmed
    ..cdc13+ cooperates with cdc2+ in the initiation of mitosis but not in the regulation of DNA replication. We propose that the cdc13+ gene product might be a G2-specific substrate of the cdc2+ protein kinase. ..
  22. Hudson J, Feilotter H, Young P. stf1: non-wee mutations epistatic to cdc25 in the fission yeast Schizosaccharomyces pombe. Genetics. 1990;126:309-15 pubmed
    ..cdc2-w, stf1- suppression of cdc25 is reversed by overexpression of the putative type 1 protein phosphatase bws1+/dis2+...