Gene Symbol: cyr1
Description: adenylate cyclase
Alias: git2, adenylate cyclase
Species: fission yeast

Top Publications

  1. Kao R, Morreale E, Wang L, Ivey F, Hoffman C. Schizosaccharomyces pombe Git1 is a C2-domain protein required for glucose activation of adenylate cyclase. Genetics. 2006;173:49-61 pubmed
    ..This requires nine git (glucose insensitive transcription) genes that encode adenylate cyclase, the PKA catalytic subunit, and seven "upstream" proteins required for glucose-triggered adenylate ..
  2. Higuchi T, Watanabe Y, Yamamoto M. Protein kinase A regulates sexual development and gluconeogenesis through phosphorylation of the Zn finger transcriptional activator Rst2p in fission yeast. Mol Cell Biol. 2002;22:1-11 pubmed
    ..These paths may ensure the specificity between the type of nutrients in shortage and the type of genes to be expressed. ..
  3. Kawamukai M, Ferguson K, Wigler M, Young D. Genetic and biochemical analysis of the adenylyl cyclase of Schizosaccharomyces pombe. Cell Regul. 1991;2:155-64 pubmed
    The adenylyl cyclase gene, cyr1, of Schizosaccharomyces pombe has been cloned...
  4. Yamawaki Kataoka Y, Tamaoki T, Choe H, Tanaka H, Kataoka T. Adenylate cyclases in yeast: a comparison of the genes from Schizosaccharomyces pombe and Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1989;86:5693-7 pubmed
    A Schizosaccharomyces pombe gene encoding adenylate cyclase has been cloned by cross-hybridization with the Saccharomyces cerevisiae adenylate cyclase gene...
  5. Maeda T, Mochizuki N, Yamamoto M. Adenylyl cyclase is dispensable for vegetative cell growth in the fission yeast Schizosaccharomyces pombe. Proc Natl Acad Sci U S A. 1990;87:7814-8 pubmed
    Disruption of the cyr1 gene of Schizosaccharomyces pombe, which encodes adenylyl cyclase, did not confer lethality to fission yeast cells, although they grew 40% slower than wild-type strains in complete medium...
  6. Goldar M, Jeong H, Tanaka K, Matsuda H, Kawamukai M. Moc3, a novel Zn finger type protein involved in sexual development, ascus formation, and stress response of Schizosaccharomyces pombe. Curr Genet. 2005;48:345-55 pubmed
    ..Thus, Moc3 is not only involved in sexual development, but also in ascus formation and DNA integrity in an independent manner with Moc1 and Moc2 in S. pombe. ..
  7. Engelberg D, Poradosu E, Simchen G, Levitzki A. Adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is not regulated by guanyl nucleotides. FEBS Lett. 1990;261:413-8 pubmed
    ..The optimal pH for the S. pombe adenylyl cyclase activity is 6.0. The activity dependence on ATP is cooperative with a Hill coefficient of 1.68 +/- 0.14. ..
  8. Jin M, Fujita M, Culley B, Apolinario E, Yamamoto M, Maundrell K, et al. sck1, a high copy number suppressor of defects in the cAMP-dependent protein kinase pathway in fission yeast, encodes a protein homologous to the Saccharomyces cerevisiae SCH9 kinase. Genetics. 1995;140:457-67 pubmed
    ..The eight git genes encode components of an adenylate cyclase activation pathway, adenylate cyclase itself, and the catalytic subunit of PKA...
  9. Stiefel J, Wang L, Kelly D, Janoo R, Seitz J, Whitehall S, et al. Suppressors of an adenylate cyclase deletion in the fission yeast Schizosaccharomyces pombe. Eukaryot Cell. 2004;3:610-9 pubmed
    ..we isolated strains carrying mutations that reduce high-level fbp1+ transcription conferred by the loss of adenylate cyclase (git2delta), including both wis1- (SAPK kinase) and spc1- (SAPK) mutants...

More Information


  1. Yamada H, Kumada K, Yanagida M. Distinct subunit functions and cell cycle regulated phosphorylation of 20S APC/cyclosome required for anaphase in fission yeast. J Cell Sci. 1997;110 ( Pt 15):1793-804 pubmed
    ..In contrast, PP1 (Dis2) phosphatase mutation shows the reverse effect on the phenotype of cut9. The Cut9 subunit is likely to be a target for regulating APC/ cyclosome function through protein-protein interactions and phosphorylation. ..
  2. Imai Y, Yamamoto M. The fission yeast mating pheromone P-factor: its molecular structure, gene structure, and ability to induce gene expression and G1 arrest in the mating partner. Genes Dev. 1994;8:328-38 pubmed
    ..Combination of the cyr1 and sxa2 mutations enhanced this susceptibility...
  3. Valbuena N, Moreno S. TOR and PKA pathways synergize at the level of the Ste11 transcription factor to prevent mating and meiosis in fission yeast. PLoS ONE. 2010;5:e11514 pubmed publisher
    ..We conclude that both the PKA and the TOR pathways inhibit Ste11 nuclear accumulation to repress Ste11-dependent gene expression. However, the PKA pathway plays a quantitatively more important role than the TOR pathway in this process. ..
  4. Ivey F, Hoffman C. Direct activation of fission yeast adenylate cyclase by the Gpa2 Galpha of the glucose signaling pathway. Proc Natl Acad Sci U S A. 2005;102:6108-13 pubmed
    ..screens and pull-down experiments, we show that the fission yeast Gpa2 Galpha binds an N-terminal domain of adenylate cyclase, comprising a moderately conserved sequence within a region otherwise poorly related to other fungal ..
  5. Demirbas D, Ceyhan O, Wyman A, Ivey F, Allain C, Wang L, et al. Use of a Schizosaccharomyces pombe PKA-repressible reporter to study cGMP metabolising phosphodiesterases. Cell Signal. 2011;23:594-601 pubmed publisher
    ..Further yeast-based assays show that BC76 inhibits PDE1, PDE4, PDE5, PDE8, PDE10 and PDE11, thus demonstrating the utility of this system for detecting and characterising inhibitors of either cAMP- or cGMP-metabolising PDEs. ..
  6. Santo P, Blanchard B, Hoffman C. The Schizosaccharomyces pombe pyp1 protein tyrosine phosphatase negatively regulates nutrient monitoring pathways. J Cell Sci. 1996;109 ( Pt 7):1919-1925 pubmed
  7. Alaamery M, Hoffman C. Schizosaccharomyces pombe Hsp90/Git10 is required for glucose/cAMP signaling. Genetics. 2008;178:1927-36 pubmed publisher
    ..activation of PKA requires the function of nine glucose-insensitive transcription (git) genes, encoding adenylate cyclase, the PKA catalytic subunit, and seven "upstream" proteins required for glucose-triggered adenylate ..
  8. Wang L, Griffiths K, Zhang Y, Ivey F, Hoffman C. Schizosaccharomyces pombe adenylate cyclase suppressor mutations suggest a role for cAMP phosphodiesterase regulation in feedback control of glucose/cAMP signaling. Genetics. 2005;171:1523-33 pubmed
    ..cAMP phosphodiesterase (PDE) gene cgs2+ were identified in a screen for suppressors of mutant alleles of the adenylate cyclase gene (git2+/cyr1+), which encode catalytically active forms of the enzyme that cannot be stimulated by ..
  9. Ogihara H, Shima F, Naito K, Asato T, Kariya K, Kataoka T. Direct activation of fission yeast adenylyl cyclase by heterotrimeric G protein gpa2. Kobe J Med Sci. 2004;50:111-21 pubmed
    Genetic studies on Schizosaccharomyces pombe adenylyl cyclase (cyr1) have shown that its activity is positively regulated by a heterotrimetric G protein a subunit gpa2 and that the resulting increase in intracellular cAMP concentration ..
  10. Caspari T. Onset of gluconate-H+ symport in Schizosaccharomyces pombe is regulated by the kinases Wis1 and Pka1, and requires the gti1+ gene product. J Cell Sci. 1997;110 ( Pt 20):2599-608 pubmed
    ..Cells which have lost either adenylate cyclase (Cyr1) or cAMP-dependent protein kinase (Pka1) transport gluconate up to 60-fold faster than wild-type cells ..
  11. Kawamukai M, Gerst J, Field J, Riggs M, Rodgers L, Wigler M, et al. Genetic and biochemical analysis of the adenylyl cyclase-associated protein, cap, in Schizosaccharomyces pombe. Mol Biol Cell. 1992;3:167-80 pubmed
    ..Our results indicate that cap is required for the proper function of S. pombe adenylyl cyclase but that the C-terminal domain of cap has other functions that are shared with the C-terminal domain of S. cerevisiae CAP. ..
  12. Hoffman C, Winston F. Glucose repression of transcription of the Schizosaccharomyces pombe fbp1 gene occurs by a cAMP signaling pathway. Genes Dev. 1991;5:561-71 pubmed
    ..In this report we demonstrate that one of these genes, git2, is the same as the cyr1 gene, which encodes adenylate cyclase, and that loss-of-function mutations in git2 cause constitutive fbp1 transcription...
  13. Landry S, Hoffman C. The git5 Gbeta and git11 Ggamma form an atypical Gbetagamma dimer acting in the fission yeast glucose/cAMP pathway. Genetics. 2001;157:1159-68 pubmed
    Fission yeast adenylate cyclase, like mammalian adenylate cyclases, is regulated by a heterotrimeric G protein. The gpa2 Galpha and git5 Gbeta are both required for glucose-triggered cAMP signaling...
  14. Davis K, Davey J. Isolation of yeast mutants hypersensitive to mating pheromones. Biochem Soc Trans. 1997;25:227S pubmed
  15. Nocero M, Isshiki T, Yamamoto M, Hoffman C. Glucose repression of fbp1 transcription of Schizosaccharomyces pombe is partially regulated by adenylate cyclase activation by a G protein alpha subunit encoded by gpa2 (git8). Genetics. 1994;138:39-45 pubmed
    ..The git2 gene, also known as cyr1, encodes adenylate cyclase. Adenylate cyclase converts ATP into the second messenger cAMP as part of many eukaryotic signal ..
  16. Yakura M, Ishikura Y, Adachi Y, Kawamukai M. Involvement of Moc1 in sexual development and survival of Schizosaccharomyces pombe. Biosci Biotechnol Biochem. 2006;70:1740-9 pubmed
    ..Thus, in addition to the roles of moc1/sds23/psp1 in mitosis and stress response, it is also important for the survival and sexual development of fission yeast, but phosphorylation of Moc1 only affects the sexual development. ..
  17. Matsuo Y, McInnis B, Marcus S. Regulation of the subcellular localization of cyclic AMP-dependent protein kinase in response to physiological stresses and sexual differentiation in the fission yeast Schizosaccharomyces pombe. Eukaryot Cell. 2008;7:1450-9 pubmed publisher
    ..Nuclear localization of both proteins is dependent on cAMP, since in cells lacking adenylate cyclase they are detectable only in the cytoplasm...
  18. Davey J, Nielsen O. Mutations in cyr1 and pat1 reveal pheromone-induced G1 arrest in the fission yeast Schizosaccharomyces pombe. Curr Genet. 1994;26:105-12 pubmed
    ..The first mutant lacks the cyr1 gene which encodes adenylate cyclase and these cells contain no measurable amounts of cAMP...
  19. Young D, Riggs M, Field J, Vojtek A, Broek D, Wigler M. The adenylyl cyclase gene from Schizosaccharomyces pombe. Proc Natl Acad Sci U S A. 1989;86:7989-93 pubmed
    ..Coussen, F., Bakalyar, H. A., Tang, W.-J., Feinstein, P. G., Orth, K., Slaughter, C., Reed, R. R. & Gilman, A. G. (1989) Science 244, 1558-1564]. ..
  20. Mudge D, Yang F, Currie B, Kim J, Yeda K, Bashyakarla V, et al. Sck1 negatively regulates Gpa2-mediated glucose signaling in Schizosaccharomyces pombe. Eukaryot Cell. 2014;13:202-8 pubmed publisher
    ..This dual role for Sck1 may allow S. pombe to produce distinct biological responses to glucose and nitrogen starvation signals that both activate the Wis1-Spc1/StyI stress-activated protein kinase (SAPK) pathway. ..