cut9

Summary

Gene Symbol: cut9
Description: anaphase-promoting complex subunit Cut9
Alias: dre1, anaphase-promoting complex subunit Cut9
Species: fission yeast

Top Publications

  1. Yamashita Y, Nakaseko Y, Samejima I, Kumada K, Yamada H, Michaelson D, et al. 20S cyclosome complex formation and proteolytic activity inhibited by the cAMP/PKA pathway. Nature. 1996;384:276-9 pubmed
    ..Suppression correlates with the amount of 20S complex, indicating that cyclosome formation and activation is inhibited by the cAMP/PKA pathway. ..
  2. He X, Patterson T, Sazer S. The Schizosaccharomyces pombe spindle checkpoint protein mad2p blocks anaphase and genetically interacts with the anaphase-promoting complex. Proc Natl Acad Sci U S A. 1997;94:7965-70 pubmed
  3. Ohi M, Feoktistova A, Ren L, Yip C, Cheng Y, Chen J, et al. Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1. Mol Cell. 2007;28:871-85 pubmed
    ..Comparison of the APC/C with another multiprotein E3 ligase, the SCF complex, uncovers remarkable structural similarities. ..
  4. Samejima I, Yanagida M. Bypassing anaphase by fission yeast cut9 mutation: requirement of cut9+ to initiate anaphase. J Cell Biol. 1994;127:1655-70 pubmed
    A novel anaphase block phenotype was found in fission yeast temperature-sensitive cut9 mutants...
  5. Sczaniecka M, Feoktistova A, May K, Chen J, Blyth J, Gould K, et al. The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C). J Biol Chem. 2008;283:23039-47 pubmed publisher
    ..Our findings demonstrate that this Mad3 KEN box is a critical mediator of Cdc20-APC/C inhibition, without which neither Mad3 nor Mad2 can associate with the APC/C or inhibit anaphase onset. ..
  6. Kim S, Lin D, Matsumoto S, Kitazono A, Matsumoto T. Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint. Science. 1998;279:1045-7 pubmed
    ..This relief of dependence appears to be a result of deregulation of ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex. ..
  7. Yamano H, Tsurumi C, Gannon J, Hunt T. The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor. EMBO J. 1998;17:5670-8 pubmed
    ..These data suggest the existence of a D-box receptor protein that recognizes D-boxes prior to ubiquitination. ..
  8. Matsumoto T. A fission yeast homolog of CDC20/p55CDC/Fizzy is required for recovery from DNA damage and genetically interacts with p34cdc2. Mol Cell Biol. 1997;17:742-50 pubmed
    ..A possible role of slp1+ is proposed. ..
  9. Berry L, Feoktistova A, Wright M, Gould K. The schizosaccharomyces pombe dim1(+) gene interacts with the anaphase-promoting complex or cyclosome (APC/C) component lid1(+) and is required for APC/C function. Mol Cell Biol. 1999;19:2535-46 pubmed
    ..of 36 degrees C, lid1-6 mutant cells arrest with a "cut" phenotype similar to that of cut4 and cut9 mutants...

More Information

Publications30

  1. Matsumura T, Yuasa T, Hayashi T, Obara T, Kimata Y, Yanagida M. A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids. Genes Cells. 2003;8:341-55 pubmed
    ..Gene cloning established that these mutants were defective in either the subunits (Cut9, Cut23, Cut20 or Apc10) of APC (anaphase promoting complex)/cyclosome or Cut8, a regulator for 26S proteasome ..
  2. Yamashita Y, Nakaseko Y, Kumada K, Nakagawa T, Yanagida M. Fission yeast APC/cyclosome subunits, Cut20/Apc4 and Cut23/Apc8, in regulating metaphase-anaphase progression and cellular stress responses. Genes Cells. 1999;4:445-63 pubmed
    ..In fission yeast, however, only five (Cut4, Cut9, Nuc2, Apc10, Hcn1) have been identified...
  3. Zhang Z, Kulkarni K, Hanrahan S, Thompson A, Barford D. The APC/C subunit Cdc16/Cut9 is a contiguous tetratricopeptide repeat superhelix with a homo-dimer interface similar to Cdc27. EMBO J. 2010;29:3733-44 pubmed publisher
    ..Here, we report the crystal structure of Schizosaccharomyces pombe Cut9 (Cdc16/Apc6) in complex with Hcn1 (Cdc26), showing that Cdc16/Cut9 is a contiguous TPR superhelix of 14 TPR units...
  4. Funabiki H, Yamano H, Kumada K, Nagao K, Hunt T, Yanagida M. Cut2 proteolysis required for sister-chromatid seperation in fission yeast. Nature. 1996;381:438-41 pubmed
    ..The rapid degradation of Cut2 at anaphase requires its amino-terminal region and the activity of Cut9 (ref...
  5. Blanco M, Sanchez Diaz A, de Prada J, Moreno S. APC(ste9/srw1) promotes degradation of mitotic cyclins in G(1) and is inhibited by cdc2 phosphorylation. EMBO J. 2000;19:3945-55 pubmed
    ..This mechanism provides a molecular switch to prevent inactivation of cdc2 in G(2) and early mitosis and to allow its inactivation in G(1). ..
  6. Yamada H, Matsumoto S, Matsumoto T. High dosage expression of a zinc finger protein, Grt1, suppresses a mutant of fission yeast slp1(+), a homolog of CDC20/p55CDC/Fizzy. J Cell Sci. 2000;113 ( Pt 22):3989-99 pubmed
    ..In cells arrested in metaphase, wild-type Slp1 was preferentially found in a complex with hyperphosphorylated Cut9 (subunit of APC), whereas the ts Slp1 protein, lacking the last 113 amino acids, failed to interact with Cut9...
  7. Bernard P, Drogat J, Maure J, Dheur S, Vaur S, Genier S, et al. A screen for cohesion mutants uncovers Ssl3, the fission yeast counterpart of the cohesin loading factor Scc4. Curr Biol. 2006;16:875-81 pubmed
    ..The discovery of a functional homolog of Scc4 indicates that the machinery of cohesin loading is conserved among eukaryotes. ..
  8. Yoon H, Feoktistova A, Chen J, Jennings J, Link A, Gould K. Role of Hcn1 and its phosphorylation in fission yeast anaphase-promoting complex/cyclosome function. J Biol Chem. 2006;281:32284-93 pubmed
    ..Phosphorylation of Hcn1 occurs at a single Cdk1 site in vitro and in vivo. Mutation of this site to alanine, but not aspartic acid, compromises APC/C function and leads to a specific defect in the completion of cell division. ..
  9. Sewart K, Hauf S. Different Functionality of Cdc20 Binding Sites within the Mitotic Checkpoint Complex. Curr Biol. 2017;27:1213-1220 pubmed publisher
  10. Tatebayashi K, Kato J, Ikeda H. Isolation of a Schizosaccharomyces pombe rad21ts mutant that is aberrant in chromosome segregation, microtubule function, DNA repair and sensitive to hydroxyurea: possible involvement of Rad21 in ubiquitin-mediated proteolysis. Genetics. 1998;148:49-57 pubmed
    ..of the rad21-K1 mutant was inhibited at the permissive temperature by introduction of another mutation in the cut9+ gene, coding for a component of the 20S cyclosome/anaphase promoting complex, which is involved in ubiquitin-..
  11. Gómez E, Angeles V, Forsburg S. A screen for Schizosaccharomyces pombe mutants defective in rereplication identifies new alleles of rad4+, cut9+ and psf2+. Genetics. 2005;169:77-89 pubmed
    ..This screen identified new alleles of the APC subunit cut9(+), the initiation/checkpoint factor rad4(+)/cut5(+), and the first mutant allele of psf2(+), a subunit of the ..
  12. Mitsuzawa H, Seino H, Yamao F, Ishihama A. Two WD repeat-containing TATA-binding protein-associated factors in fission yeast that suppress defects in the anaphase-promoting complex. J Biol Chem. 2001;276:17117-24 pubmed
    ..the cell cycle arrest in mitosis caused by a mutation in the anaphase-promoting complex/cyclosome subunit gene cut9(+)...
  13. Dubey R, Nakwal N, Bisht K, Saini A, Haldar S, Singh J. Interaction of APC/C-E3 ligase with Swi6/HP1 and Clr4/Suv39 in heterochromatin assembly in fission yeast. J Biol Chem. 2009;284:7165-76 pubmed publisher
    ..Swi6, H3-Lys-9-Me2, and Clr4 at dg-dh repeats at cen1 and the K region of mat locus is abrogated in mutants cut4, cut9, and nuc2...
  14. Kimata Y, Matsuyama A, Nagao K, Furuya K, Obuse C, Yoshida M, et al. Diminishing HDACs by drugs or mutations promotes normal or abnormal sister chromatid separation by affecting APC/C and adherin. J Cell Sci. 2008;121:1107-18 pubmed publisher
    ..HATs and HDACs might directly target adherin and APC/C to ensure proper chromosome segregation, and anti-tumour effects of HDAC inhibitors could be attributed to this deregulation. ..
  15. Osaka F, Seino H, Seno T, Yamao F. A ubiquitin-conjugating enzyme in fission yeast that is essential for the onset of anaphase in mitosis. Mol Cell Biol. 1997;17:3388-97 pubmed
    ..Overexpression of UbcP4 specifically rescued the growth defect of cut9ts cells at a restrictive temperature. cut9 encodes a component of the anaphase-promoting complex (APC) which is required for chromosome segregation at ..
  16. Elmore Z, Beckley J, Chen J, Gould K. Histone H2B ubiquitination promotes the function of the anaphase-promoting complex/cyclosome in Schizosaccharomyces pombe. G3 (Bethesda). 2014;4:1529-38 pubmed publisher
    ..transferase (SAGA) complex associated DUB, suppressed temperature-sensitive phenotypes of APC/C mutants cut9-665, lid1-6, cut4-533, and slp1-362...
  17. Ishii K, Kumada K, Toda T, Yanagida M. Requirement for PP1 phosphatase and 20S cyclosome/APC for the onset of anaphase is lessened by the dosage increase of a novel gene sds23+. EMBO J. 1996;15:6629-40 pubmed
    ..These results demonstrate functions other than promoting anaphase for the components of the 20S cyclosome/APC and also a close functional relationship of Sds23 with PP1 and 20S cyclosome/APC. ..
  18. Seeger M, Hartmann Petersen R, Wilkinson C, Wallace M, Samejima I, Taylor M, et al. Interaction of the anaphase-promoting complex/cyclosome and proteasome protein complexes with multiubiquitin chain-binding proteins. J Biol Chem. 2003;278:16791-6 pubmed
    ..The putative role of Pus1 as a factor involved in allocation of ubiquitinylated substrates for the proteasome is discussed. ..
  19. Kimata Y, Yanagida M. Suppression of a mitotic mutant by tRNA-Ala anticodon mutations that produce a dominant defect in late mitosis. J Cell Sci. 2004;117:2283-93 pubmed
    ..mutants scn1 and scn2 of Schizosaccharomyces pombe were isolated by their ability to suppress temperature-sensitive cut9-665 defective in an essential subunit (human Apc6/budding yeast Cdc16 ortholog) of anaphase promoting complex/..
  20. Tatebe H, Yanagida M. Cut8, essential for anaphase, controls localization of 26S proteasome, facilitating destruction of cyclin and Cut2. Curr Biol. 2000;10:1329-38 pubmed
    ..Cut8 is a novel evolutionarily conserved heat-inducible regulator. It facilitates anaphase-promoting proteolysis by recruiting 26S proteasome to a functionally efficient nuclear location. ..
  21. Yamada H, Kumada K, Yanagida M. Distinct subunit functions and cell cycle regulated phosphorylation of 20S APC/cyclosome required for anaphase in fission yeast. J Cell Sci. 1997;110 ( Pt 15):1793-804 pubmed
    We show here that the fission yeast gene products Cut9 and Nuc2 are the subunits of the 20S complex, the putative APC (anaphase promoting complex)/cyclosome which contains ubiquitin ligase activity required for cyclin and Cut2 destruction...