cut4

Summary

Gene Symbol: cut4
Description: anaphase-promoting complex subunit Apc1
Alias: apc1, anaphase-promoting complex subunit Apc1
Species: fission yeast

Top Publications

  1. Yamashita Y, Nakaseko Y, Samejima I, Kumada K, Yamada H, Michaelson D, et al. 20S cyclosome complex formation and proteolytic activity inhibited by the cAMP/PKA pathway. Nature. 1996;384:276-9 pubmed
    ..The formation and activation of the 20S cyclosome complex is regulated by an unknown mechanism. Here we show that Cut4 (ref. 6) is an essential component of the cyclosome in fission yeast...
  2. Kim S, Lin D, Matsumoto S, Kitazono A, Matsumoto T. Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint. Science. 1998;279:1045-7 pubmed
    ..This relief of dependence appears to be a result of deregulation of ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex. ..
  3. Ohi M, Feoktistova A, Ren L, Yip C, Cheng Y, Chen J, et al. Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1. Mol Cell. 2007;28:871-85 pubmed
    ..Comparison of the APC/C with another multiprotein E3 ligase, the SCF complex, uncovers remarkable structural similarities. ..
  4. Sczaniecka M, Feoktistova A, May K, Chen J, Blyth J, Gould K, et al. The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C). J Biol Chem. 2008;283:23039-47 pubmed publisher
    ..Our findings demonstrate that this Mad3 KEN box is a critical mediator of Cdc20-APC/C inhibition, without which neither Mad3 nor Mad2 can associate with the APC/C or inhibit anaphase onset. ..
  5. Seeger M, Hartmann Petersen R, Wilkinson C, Wallace M, Samejima I, Taylor M, et al. Interaction of the anaphase-promoting complex/cyclosome and proteasome protein complexes with multiubiquitin chain-binding proteins. J Biol Chem. 2003;278:16791-6 pubmed
    ..sequences also found in the proteasome subunit SpRpn2 and the anaphase-promoting complex/cyclosome (APC/C) subunit Cut4. The putative role of Pus1 as a factor involved in allocation of ubiquitinylated substrates for the proteasome is ..
  6. Yoon H, Feoktistova A, Chen J, Jennings J, Link A, Gould K. Role of Hcn1 and its phosphorylation in fission yeast anaphase-promoting complex/cyclosome function. J Biol Chem. 2006;281:32284-93 pubmed
    ..Phosphorylation of Hcn1 occurs at a single Cdk1 site in vitro and in vivo. Mutation of this site to alanine, but not aspartic acid, compromises APC/C function and leads to a specific defect in the completion of cell division. ..
  7. Tatebe H, Yanagida M. Cut8, essential for anaphase, controls localization of 26S proteasome, facilitating destruction of cyclin and Cut2. Curr Biol. 2000;10:1329-38 pubmed
    ..Cut8 is a novel evolutionarily conserved heat-inducible regulator. It facilitates anaphase-promoting proteolysis by recruiting 26S proteasome to a functionally efficient nuclear location. ..
  8. Spirek M, Estreicher A, Csaszar E, Wells J, McFarlane R, Watts F, et al. SUMOylation is required for normal development of linear elements and wild-type meiotic recombination in Schizosaccharomyces pombe. Chromosoma. 2010;119:59-72 pubmed publisher
    ..On the side, MS analysis confirmed the interaction of Rec10 with the known LinE components Rec25, Rec27, and Hop1 and identified the meiotically upregulated protein Mug20 as a novel putative LinE-associated protein. ..
  9. Yamashita Y, Nakaseko Y, Kumada K, Nakagawa T, Yanagida M. Fission yeast APC/cyclosome subunits, Cut20/Apc4 and Cut23/Apc8, in regulating metaphase-anaphase progression and cellular stress responses. Genes Cells. 1999;4:445-63 pubmed
    ..In fission yeast, however, only five (Cut4, Cut9, Nuc2, Apc10, Hcn1) have been identified...

More Information

Publications15

  1. Matsumura T, Yuasa T, Hayashi T, Obara T, Kimata Y, Yanagida M. A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids. Genes Cells. 2003;8:341-55 pubmed
    ..In addition, the manner of the inhibition by high copy securin and separase plasmids were quite different each other and did not result in the mitotic block. ..
  2. Ikai N, Yanagida M. Cdc48 is required for the stability of Cut1/separase in mitotic anaphase. J Struct Biol. 2006;156:50-61 pubmed
    ..Cells lose viability during the traverse of anaphase in cdc48 mutant cells. Cdc48 may protect Cut1/separase and Cut2/securin against the instability during polyubiquitination and degradation in the metaphase-anaphase transition. ..
  3. Kimata Y, Matsuyama A, Nagao K, Furuya K, Obuse C, Yoshida M, et al. Diminishing HDACs by drugs or mutations promotes normal or abnormal sister chromatid separation by affecting APC/C and adherin. J Cell Sci. 2008;121:1107-18 pubmed publisher
    ..HATs and HDACs might directly target adherin and APC/C to ensure proper chromosome segregation, and anti-tumour effects of HDAC inhibitors could be attributed to this deregulation. ..
  4. Dubey R, Nakwal N, Bisht K, Saini A, Haldar S, Singh J. Interaction of APC/C-E3 ligase with Swi6/HP1 and Clr4/Suv39 in heterochromatin assembly in fission yeast. J Biol Chem. 2009;284:7165-76 pubmed publisher
    ..of Swi6, H3-Lys-9-Me2, and Clr4 at dg-dh repeats at cen1 and the K region of mat locus is abrogated in mutants cut4, cut9, and nuc2...
  5. Kimata Y, Kitamura K, Fenner N, Yamano H. Mes1 controls the meiosis I to meiosis II transition by distinctly regulating the anaphase-promoting complex/cyclosome coactivators Fzr1/Mfr1 and Slp1 in fission yeast. Mol Biol Cell. 2011;22:1486-94 pubmed publisher
    ..Thus Mes1 interacts with two coactivators in a different way to control the activity of the APC/C required for the meiosis I/meiosis II transition...
  6. Elmore Z, Beckley J, Chen J, Gould K. Histone H2B ubiquitination promotes the function of the anaphase-promoting complex/cyclosome in Schizosaccharomyces pombe. G3 (Bethesda). 2014;4:1529-38 pubmed publisher
    ..SAGA) complex associated DUB, suppressed temperature-sensitive phenotypes of APC/C mutants cut9-665, lid1-6, cut4-533, and slp1-362...