Gene Symbol: ctp1
Description: CtIP family endonuclease
Alias: mug38, nip1, slr9, CtIP family endonuclease
Species: fission yeast

Top Publications

  1. Lengsfeld B, Rattray A, Bhaskara V, Ghirlando R, Paull T. Sae2 is an endonuclease that processes hairpin DNA cooperatively with the Mre11/Rad50/Xrs2 complex. Mol Cell. 2007;28:638-51 pubmed
  2. Hartsuiker E, Mizuno K, Molnar M, Kohli J, Ohta K, Carr A. Ctp1CtIP and Rad32Mre11 nuclease activity are required for Rec12Spo11 removal, but Rec12Spo11 removal is dispensable for other MRN-dependent meiotic functions. Mol Cell Biol. 2009;29:1671-81 pubmed publisher
    ..Furthermore, we show that the CtIP homologue Ctp1 is required for Rec12(Spo11) removal, confirming functional conservation between Ctp1(CtIP) and the more distantly ..
  3. Farah J, Cromie G, Smith G. Ctp1 and Exonuclease 1, alternative nucleases regulated by the MRN complex, are required for efficient meiotic recombination. Proc Natl Acad Sci U S A. 2009;106:9356-61 pubmed publisher
    ..We find that in the presence of the MRN (Rad32-Rad50-Nbs1) complex efficient recombination requires Ctp1, the ortholog of the nuclease Sae2, but not the nuclease activity of MRN...
  4. Limbo O, Porter Goff M, Rhind N, Russell P. Mre11 nuclease activity and Ctp1 regulate Chk1 activation by Rad3ATR and Tel1ATM checkpoint kinases at double-strand breaks. Mol Cell Biol. 2011;31:573-83 pubmed publisher
    ..MRN) nucleolytic protein complex in association with the Tel1(ATM) checkpoint kinase and the Ctp1(CtIP/Sae2) DNA-end processing factor; however, in budding yeast, neither Mre11 nuclease activity or Sae2 are ..
  5. Akamatsu Y, Murayama Y, Yamada T, Nakazaki T, Tsutsui Y, Ohta K, et al. Molecular characterization of the role of the Schizosaccharomyces pombe nip1+/ctp1+ gene in DNA double-strand break repair in association with the Mre11-Rad50-Nbs1 complex. Mol Cell Biol. 2008;28:3639-51 pubmed publisher
    The Schizosaccharomyces pombe nip1(+)/ctp1(+) gene was previously identified as an slr (synthetically lethal with rad2) mutant...
  6. Hartsuiker E, Neale M, Carr A. Distinct requirements for the Rad32(Mre11) nuclease and Ctp1(CtIP) in the removal of covalently bound topoisomerase I and II from DNA. Mol Cell. 2009;33:117-23 pubmed publisher
    ..A ctp1(CtIP) deletion is defective for Top2 removal but overproficient for Top1 removal, suggesting that Ctp1(CtIP) plays ..
  7. Rothenberg M, Kohli J, Ludin K. Ctp1 and the MRN-complex are required for endonucleolytic Rec12 removal with release of a single class of oligonucleotides in fission yeast. PLoS Genet. 2009;5:e1000722 pubmed publisher
    ..b>Ctp1, Rad50, and the nuclease activity of Rad32, the fission yeast homolog of Mre11, are required for endonucleolytic ..
  8. Limbo O, Chahwan C, Yamada Y, de Bruin R, Wittenberg C, Russell P. Ctp1 is a cell-cycle-regulated protein that functions with Mre11 complex to control double-strand break repair by homologous recombination. Mol Cell. 2007;28:134-46 pubmed
    ..Here we describe fission yeast Ctp1, so-named because it shares conserved domains with the mammalian tumor suppressor CtIP...
  9. Milman N, Higuchi E, Smith G. Meiotic DNA double-strand break repair requires two nucleases, MRN and Ctp1, to produce a single size class of Rec12 (Spo11)-oligonucleotide complexes. Mol Cell Biol. 2009;29:5998-6005 pubmed publisher
    ..Rec12-oligonucleotide generation strictly requires Ctp1 (Sae2 nuclease homolog), the Rad32 (Mre11) nuclease domain, and Rad50 of the MRN complex...

More Information


  1. Caetano C, Limbo O, Farmer S, Klier S, Dovey C, Russell P, et al. Tolerance of deregulated G1/S transcription depends on critical G1/S regulon genes to prevent catastrophic genome instability. Cell Rep. 2014;9:2279-89 pubmed publisher
    ..Homology-directed repair proteins, including MBF-regulated Ctp1(CtIP), are essential to prevent catastrophic genome instability...
  2. Williams R, Dodson G, Limbo O, Yamada Y, Williams J, Guenther G, et al. Nbs1 flexibly tethers Ctp1 and Mre11-Rad50 to coordinate DNA double-strand break processing and repair. Cell. 2009;139:87-99 pubmed publisher
    ..double-strand break (DSB) repair and checkpoint signaling through undefined interactions with ATM, MDC1, and Sae2/Ctp1/CtIP...
  3. Andres S, Williams R. CtIP/Ctp1/Sae2, molecular form fit for function. DNA Repair (Amst). 2017;56:109-117 pubmed publisher
    Vertebrate CtIP, and its fission yeast (Ctp1), budding yeast (Sae2) and plant (Com1) orthologs have emerged as key regulatory molecules in cellular responses to DNA double strand breaks (DSBs)...
  4. Langerak P, Mejía Ramírez E, Limbo O, Russell P. Release of Ku and MRN from DNA ends by Mre11 nuclease activity and Ctp1 is required for homologous recombination repair of double-strand breaks. PLoS Genet. 2011;7:e1002271 pubmed publisher
    The multifunctional Mre11-Rad50-Nbs1 (MRN) protein complex recruits ATM/Tel1 checkpoint kinase and CtIP/Ctp1 homologous recombination (HR) repair factor to double-strand breaks (DSBs)...
  5. Li Y, Wang J, Zhou G, Lajeunesse M, Le N, Stawicki B, et al. Nonhomologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex in Schizosaccharomyces pombe. Genetics. 2017;206:481-496 pubmed publisher
    ..pombe NHEJ was reduced >1000-fold in cells lacking each MRN subunit, and loss of MRN-associated Ctp1 caused a 30-fold reduction...
  6. Porter Goff M, Rhind N. The role of MRN in the S-phase DNA damage checkpoint is independent of its Ctp1-dependent roles in double-strand break repair and checkpoint signaling. Mol Biol Cell. 2009;20:2096-107 pubmed publisher
    ..Mechanistically, MRN, along with its cofactor Ctp1, is involved in 5' resection to create single-stranded DNA that is required for both signaling and homologous ..
  7. S nchez A, Sharma S, Rozenzhak S, Roguev A, Krogan N, Chabes A, et al. Replication fork collapse and genome instability in a deoxycytidylate deaminase mutant. Mol Cell Biol. 2012;32:4445-54 pubmed publisher
    ..Moreover, Brc1 suppresses spontaneous mutagenesis in dcd1? cells. We propose that replication forks stall and collapse in dcd1? cells, burdening DNA damage and checkpoint responses to maintain genome integrity...
  8. Lloyd J, Chapman J, Clapperton J, Haire L, Hartsuiker E, Li J, et al. A supramodular FHA/BRCT-repeat architecture mediates Nbs1 adaptor function in response to DNA damage. Cell. 2009;139:100-11 pubmed publisher
    ..Furthermore, we show that similar phosphomotifs within Ctp1, the fission yeast ortholog of human CtIP, promote interactions with the Nbs1 FHA domain that are necessary for ..
  9. Hentges P, Waller H, Reis C, Ferreira M, Doherty A. Cdk1 restrains NHEJ through phosphorylation of XRCC4-like factor Xlf1. Cell Rep. 2014;9:2011-7 pubmed publisher
  10. Dodson G, Limbo O, Nieto D, Russell P. Phosphorylation-regulated binding of Ctp1 to Nbs1 is critical for repair of DNA double-strand breaks. Cell Cycle. 2010;9:1516-22 pubmed
    ..In Schizosaccharomyces pombe, the Mre11-Rad50-Nbs1 (MRN) complex and Ctp1 cooperate to perform the initial steps that process and repair these DNA lesions via homologous recombination (HR)...
  11. Ma L, Milman N, Nambiar M, Smith G. Two separable functions of Ctp1 in the early steps of meiotic DNA double-strand break repair. Nucleic Acids Res. 2015;43:7349-59 pubmed publisher
    ..b>Ctp1 (Com1, Sae2, CtIP homolog) acting with the Mre11-Rad50-Nbs1 (MRN) complex is required in both steps...
  12. Andres S, Appel C, Westmoreland J, Williams J, Nguyen Y, Robertson P, et al. Tetrameric Ctp1 coordinates DNA binding and DNA bridging in DNA double-strand-break repair. Nat Struct Mol Biol. 2015;22:158-66 pubmed publisher
    b>Ctp1 (also known as CtIP or Sae2) collaborates with Mre11-Rad50-Nbs1 to initiate repair of DNA double-strand breaks (DSBs), but its functions remain enigmatic...
  13. Teixeira Silva A, Ait Saada A, Hardy J, Iraqui I, Nocente M, Fréon K, et al. The end-joining factor Ku acts in the end-resection of double strand break-free arrested replication forks. Nat Commun. 2017;8:1982 pubmed publisher
    ..An initial resection mediated by MRN-Ctp1 removes Ku from terminally arrested forks, generating ~110?bp sized gaps obligatory for subsequent Exo1-mediated ..
  14. Almeida H, Godinho Ferreira M. Spontaneous telomere to telomere fusions occur in unperturbed fission yeast cells. Nucleic Acids Res. 2013;41:3056-67 pubmed publisher
    ..occurred via microhomology-mediated end-joining (MMEJ)/single-strand annealing (SSA) repair and also required MRN/Ctp1. Strikingly, we were able to capture spontaneous telomere-to-telomere fusions in unperturbed cells...