crm1

Summary

Gene Symbol: crm1
Description: importin family nuclear export receptor Crm1
Alias: SPAC1B2.01, caf2, importin family nuclear export receptor Crm1
Species: fission yeast

Top Publications

  1. Adachi Y, Yanagida M. Higher order chromosome structure is affected by cold-sensitive mutations in a Schizosaccharomyces pombe gene crm1+ which encodes a 115-kD protein preferentially localized in the nucleus and its periphery. J Cell Biol. 1989;108:1195-207 pubmed
    ..Their mutations were mapped in a novel, identical locus designated crm1 (chromosomal region maintenance). The crm1 mutants also show the following phenotypes...
  2. Ling Y, Vjestica A, Oliferenko S. Nucleocytoplasmic shuttling of the TACC protein Mia1p/Alp7p is required for remodeling of microtubule arrays during the cell cycle. PLoS ONE. 2009;4:e6255 pubmed publisher
    ..We propose that intricate regulation of the subcellular distribution of TACC/TOG complexes drives microtubule array remodeling as cells progress through the cell cycle. ..
  3. Benko Z, Fenyvesvolgyi C, Pesti M, Sipiczki M. The transcription factor Pap1/Caf3 plays a central role in the determination of caffeine resistance in Schizosaccharomyces pombe. Mol Genet Genomics. 2004;271:161-70 pubmed
    ..caf2+ and caf4+ were sequenced and found to be identical to the multidrug-resistance/stress-response genes hba1, crm1 and trr1, respectively. Here we show that caf3 is allelic to pap1, which encodes an AP-1-like transcription factor...
  4. Castillo E, Ayte J, Chiva C, Moldón A, Carrascal M, Abian J, et al. Diethylmaleate activates the transcription factor Pap1 by covalent modification of critical cysteine residues. Mol Microbiol. 2002;45:243-54 pubmed
    ..This modification impedes the interaction of the nuclear exporter Crm1 with the nuclear export signal located at the carboxy-terminal domain of Pap1...
  5. Pasion S, Forsburg S. Nuclear localization of Schizosaccharomyces pombe Mcm2/Cdc19p requires MCM complex assembly. Mol Biol Cell. 1999;10:4043-57 pubmed
    ..We suggest that coupling complex assembly to nuclear targeting and retention ensures that only intact heterohexameric MCM complexes remain nuclear. ..
  6. Tange Y, Hirata A, Niwa O. An evolutionarily conserved fission yeast protein, Ned1, implicated in normal nuclear morphology and chromosome stability, interacts with Dis3, Pim1/RCC1 and an essential nucleoporin. J Cell Sci. 2002;115:4375-85 pubmed
    ..of genetic interactions with factors involved in nuclear transport and chromosome segregation, including the crm1 (exportin), spi1 (small GTPase Ran), pim1, and dis genes...
  7. Santosa V, Martha S, Hirose N, Tanaka K. The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication. J Biol Chem. 2013;288:6864-80 pubmed publisher
    ..These results strongly suggest that Mcb1 promotes efficient pre-RC formation during DNA replication by regulating the MCM complex...
  8. Gaits F, Russell P. Active nucleocytoplasmic shuttling required for function and regulation of stress-activated kinase Spc1/StyI in fission yeast. Mol Biol Cell. 1999;10:1395-407 pubmed
    ..Nuclear export of Spc1 is regulated by the export factor Crm1. An Spc1-Crm1 complex forms as Spc1 is exported from the nucleus...
  9. Toone W, Kuge S, Samuels M, Morgan B, Toda T, Jones N. Regulation of the fission yeast transcription factor Pap1 by oxidative stress: requirement for the nuclear export factor Crm1 (Exportin) and the stress-activated MAP kinase Sty1/Spc1. Genes Dev. 1998;12:1453-63 pubmed
    ..This relocalization is the result of regulated protein export, rather than import, and involves the Crm1 (exportin) nuclear export factor and the dcd1+/pim1+ gene that encodes an Ran nucleotide exchange factor.

More Information

Publications23

  1. Kudo N, Khochbin S, Nishi K, Kitano K, Yanagida M, Yoshida M, et al. Molecular cloning and cell cycle-dependent expression of mammalian CRM1, a protein involved in nuclear export of proteins. J Biol Chem. 1997;272:29742-51 pubmed
    b>Crm1 of Schizosaccharomyces pombe, a nuclear protein essential for proliferation and chromosome region maintenance, is a possible target of leptomycin B, an antifungal and antitumor antibiotic with cell cycle-arresting activity...
  2. Kumada K, Yanagida M, Toda T. Caffeine-resistance in fission yeast is caused by mutations in a single essential gene, crm1+. Mol Gen Genet. 1996;250:59-68 pubmed
    ..Surprisingly, all twelve ts isolates contained a mutation in the same locus, crm1. Cells of the ts crm1 mutant showed an abnormal chromosome structure at the restrictive temperature, an elevated ..
  3. Neville M, Stutz F, Lee L, Davis L, Rosbash M. The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export. Curr Biol. 1997;7:767-75 pubmed
    ..Here, we have studied this mechanism of nuclear to cytoplasmic transport. Viable mis-sense mutations in the CRM1 gene substantially reduced or eliminated the biological activity of Rev in S...
  4. Toda T, Shimanuki M, Saka Y, Yamano H, Adachi Y, Shirakawa M, et al. Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1. Mol Cell Biol. 1992;12:5474-84 pubmed
    ..p25 was previously found to be overproduced in strains carrying cold-sensitive crm1 mutations whose gene product is essential for viability and is thought to play an important role in maintenance of ..
  5. Castillo E, Vivancos A, Jones N, Ayte J, Hidalgo E. Schizosaccharomyces pombe cells lacking the Ran-binding protein Hba1 show a multidrug resistance phenotype due to constitutive nuclear accumulation of Pap1. J Biol Chem. 2003;278:40565-72 pubmed
    ..the transcription factor Pap1, and the mitogen-activated protein kinase Sty1 are excluded from the nucleus in a Crm1-dependent manner under non-stressed conditions...
  6. Mercier A, Labbe S. Both Php4 function and subcellular localization are regulated by iron via a multistep mechanism involving the glutaredoxin Grx4 and the exportin Crm1. J Biol Chem. 2009;284:20249-62 pubmed publisher
    ..This latter process was blocked by leptomycin B. By using coimmunoprecipitation analysis, we showed that Php4 and Crm1 physically interact with each other...
  7. Kudo N, Matsumori N, Taoka H, Fujiwara D, Schreiner E, Wolff B, et al. Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region. Proc Natl Acad Sci U S A. 1999;96:9112-7 pubmed
    The cellular target of leptomycin B (LMB), a nuclear export inhibitor, has been identified as CRM1 (exportin 1), an evolutionarily conserved receptor for the nuclear export signal of proteins...
  8. Nishijima H, Nakayama J, Yoshioka T, Kusano A, Nishitani H, Shibahara K, et al. Nuclear RanGAP is required for the heterochromatin assembly and is reciprocally regulated by histone H3 and Clr4 histone methyltransferase in Schizosaccharomyces pombe. Mol Biol Cell. 2006;17:2524-36 pubmed
    ..We also found that SpRna1 formed a stable complex with Xpo1/Crm1 plus Ran-GTP, in the presence of H3.
  9. Beaudoin J, Labbe S. Crm1-mediated nuclear export of the Schizosaccharomyces pombe transcription factor Cuf1 during a shift from low to high copper concentrations. Eukaryot Cell. 2007;6:764-75 pubmed
    ..the wild-type Cuf1 protein was inhibited by leptomycin B (LMB), a specific inhibitor of the nuclear export protein Crm1. We further show that cells expressing a temperature-sensitive mutation in crm1(+) exhibit increased nuclear ..
  10. Benko Z, Miklos I, Carr A, Sipiczki M. Caffeine-resistance in S. pombe: mutations in three novel caf genes increase caffeine tolerance and affect radiation sensitivity, fertility, and cell cycle. Curr Genet. 1997;31:481-7 pubmed
    ..We have isolated novel caffeine-resistant mutants of S. pombe which define three distinct genes caf2, caf3 and caf4...
  11. Takeda K, Yoshida T, Kikuchi S, Nagao K, Kokubu A, Pluskal T, et al. Synergistic roles of the proteasome and autophagy for mitochondrial maintenance and chronological lifespan in fission yeast. Proc Natl Acad Sci U S A. 2010;107:3540-5 pubmed publisher
    ..Proteasome and autophagy are thus collaborative to support the lifespan of S. pombe G0 phase. ..
  12. Muñoz S, Manjón E, Garcia P, Sunnerhagen P, Sanchez Y. The checkpoint-dependent nuclear accumulation of Rho1p exchange factor Rgf1p is important for tolerance to chronic replication stress. Mol Biol Cell. 2014;25:1137-50 pubmed publisher
    ..It will be of general interest to understand whether the same is true for homologues of Rgf1p in budding yeast and higher eukaryotes. ..
  13. Satoh R, Matsumura Y, Tanaka A, Takada M, Ito Y, Hagihara K, et al. Spatial regulation of the KH domain RNA-binding protein Rnc1 mediated by a Crm1-independent nuclear export system in Schizosaccharomyces pombe. Mol Microbiol. 2017;104:428-448 pubmed publisher
    ..a putative nuclear export signal (NES)Rnc1 , which dictates the cytoplasmic localization of Rnc1 in a Crm1-independent manner...
  14. Clasen S, Shao W, Gu H, Espenshade P. Prolyl dihydroxylation of unassembled uS12/Rps23 regulates fungal hypoxic adaptation. elife. 2017;6: pubmed publisher
    ..To date, oxygen is the only known signal to Sre1, but additional nutrient signals may tune the hypoxic response through control of unassembled Rps23 or Ofd1 activity. ..