Gene Symbol: cds1
Description: replication checkpoint kinase cds1
Alias: replication checkpoint kinase cds1
Species: fission yeast

Top Publications

  1. Noguchi E, Noguchi C, McDonald W, Yates J, Russell P. Swi1 and Swi3 are components of a replication fork protection complex in fission yeast. Mol Cell Biol. 2004;24:8342-55 pubmed
    ..The Swi1-Swi3 complex is required for survival of fork arrest and for activation of the replication checkpoint kinase Cds1. Association of Swi1 and Swi3 with chromatin during DNA replication correlated with movement of the ..
  2. Willis N, Rhind N. Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpoint. Mol Biol Cell. 2009;20:819-33 pubmed publisher
    ..In the fission yeast Schizosaccharomyces pombe, Cds1, the S-phase-specific checkpoint effector kinase, is required for checkpoint signaling and replication slowing; ..
  3. Tanaka K, Russell P. Cds1 phosphorylation by Rad3-Rad26 kinase is mediated by forkhead-associated domain interaction with Mrc1. J Biol Chem. 2004;279:32079-86 pubmed
    The protein kinase Cds1 is an effector of the replication checkpoint in the fission yeast Schizosaccharomyces pombe...
  4. Boddy M, Furnari B, Mondesert O, Russell P. Replication checkpoint enforced by kinases Cds1 and Chk1. Science. 1998;280:909-12 pubmed
    ..Treatment of fission yeast with HU stimulated the kinase Cds1, which appears to phosphorylate the kinase Wee1, an inhibitor of Cdc2...
  5. Xu Y, Davenport M, Kelly T. Two-stage mechanism for activation of the DNA replication checkpoint kinase Cds1 in fission yeast. Genes Dev. 2006;20:990-1003 pubmed
    ..In Schizosaccharomyces pombe the major effector of the replication checkpoint is the protein kinase Cds1. Activation of Cds1 is known to require the upstream kinase Rad3 and the mediator Mrc1, but the biochemical ..
  6. Tanaka K, Boddy M, Chen X, McGowan C, Russell P. Threonine-11, phosphorylated by Rad3 and atm in vitro, is required for activation of fission yeast checkpoint kinase Cds1. Mol Cell Biol. 2001;21:3398-404 pubmed
    Fission yeast Cds1 is phosphorylated and activated when DNA replication is interrupted by nucleotide starvation or DNA damage. Cds1 enforces the S-M checkpoint that couples mitosis (M) to the completion of DNA synthesis (S)...
  7. Murakami H, Nurse P. Meiotic DNA replication checkpoint control in fission yeast. Genes Dev. 1999;13:2581-93 pubmed
    ..The mitotic checkpoint Rad genes and the Cds1 protein kinase are required for the DNA replication checkpoint during meiosis, with Cds1 playing a more prominent ..
  8. Zhao H, Tanaka K, Nogochi E, Nogochi C, Russell P. Replication checkpoint protein Mrc1 is regulated by Rad3 and Tel1 in fission yeast. Mol Cell Biol. 2003;23:8395-403 pubmed
    ..checkpoint 1) is an adaptor checkpoint protein required for Rad3-dependent activation of the checkpoint kinase Cds1 in response to arrest of replication forks. Here we report studies on the regulation of Mrc1 by phosphorylation...
  9. Snaith H, Brown G, Forsburg S. Schizosaccharomyces pombe Hsk1p is a potential cds1p target required for genome integrity. Mol Cell Biol. 2000;20:7922-32 pubmed
    ..These results indicate that the Hsk1p kinase is a potential target of Cds1p regulation and that its activity is required after replication initiation for normal mitosis. ..

More Information


  1. Nitani N, Nakamura K, Nakagawa C, Masukata H, Nakagawa T. Regulation of DNA replication machinery by Mrc1 in fission yeast. Genetics. 2006;174:155-65 pubmed
    ..hypersensitivity in the absence of Chk1, a DNA damage checkpoint kinase, and were defective in full activation of Cds1, a replication checkpoint kinase, indicating that normal replication is required for Cds1 activation...
  2. Furnari B, Blasina A, Boddy M, McGowan C, Russell P. Cdc25 inhibited in vivo and in vitro by checkpoint kinases Cds1 and Chk1. Mol Biol Cell. 1999;10:833-45 pubmed
    In the fission yeast Schizosaccharomyces pombe, the protein kinase Cds1 is activated by the S-M replication checkpoint that prevents mitosis when DNA is incompletely replicated...
  3. Aono N, Sutani T, Tomonaga T, Mochida S, Yanagida M. Cnd2 has dual roles in mitotic condensation and interphase. Nature. 2002;417:197-202 pubmed
    ..A definitive defect of interphase is abolishment of Cds1 (a checkpoint kinase) activation in the presence of hydroxyurea in both cnd2-1 mutant cells and in cells where ..
  4. Martinho R, Lindsay H, Flaggs G, DeMaggio A, Hoekstra M, Carr A, et al. Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses. EMBO J. 1998;17:7239-49 pubmed
    ..During S phase, the Cds1 kinase is activated in response to DNA damage and DNA replication blocks...
  5. Sommariva E, Pellny T, Karahan N, Kumar S, Huberman J, Dalgaard J. Schizosaccharomyces pombe Swi1, Swi3, and Hsk1 are components of a novel S-phase response pathway to alkylation damage. Mol Cell Biol. 2005;25:2770-84 pubmed
    ..additive effects on MMS sensitivity and on the MMS-induced damage checkpoint response when combined with chk1 and cds1, but they are nonadditive with hsk1...
  6. Dutta C, Rhind N. The role of specific checkpoint-induced S-phase transcripts in resistance to replicative stress. PLoS ONE. 2009;4:e6944 pubmed publisher
    Checkpoint activation during S phase modulates transcription. In response to replication arrest, the fission yeast Cds1 checkpoint kinase maintains the normal S-phase transcriptional program by regulating MBF, the S-phase transcription ..
  7. Dutta C, Patel P, Rosebrock A, Oliva A, Leatherwood J, Rhind N. The DNA replication checkpoint directly regulates MBF-dependent G1/S transcription. Mol Cell Biol. 2008;28:5977-85 pubmed publisher
    ..We propose a mechanism for this regulation, based on in vitro phosphorylation of the Cdc10 subunit of MBF by the Cds1 replication-checkpoint kinase...
  8. de Bruin R, Kalashnikova T, Aslanian A, Wohlschlegel J, Chahwan C, Yates J, et al. DNA replication checkpoint promotes G1-S transcription by inactivating the MBF repressor Nrm1. Proc Natl Acad Sci U S A. 2008;105:11230-5 pubmed publisher
    ..Phosphorylation of Nrm1 by the Cds1 (Chk2) checkpoint protein kinase, which is activated in response to DNA replication stress, promotes its ..
  9. Matsuura A, Naito T, Ishikawa F. Genetic control of telomere integrity in Schizosaccharomyces pombe: rad3(+) and tel1(+) are parts of two regulatory networks independent of the downstream protein kinases chk1(+) and cds1(+). Genetics. 1999;152:1501-12 pubmed
    ..Previous studies have shown that Rad3 fulfills its function via the regulation of the Chk1 and Cds1 protein kinases. Here we describe a novel role for Rad3 in the control of telomere integrity...
  10. Dovey C, Aslanian A, Sofueva S, Yates J, Russell P. Mms1-Mms22 complex protects genome integrity in Schizosaccharomyces pombe. DNA Repair (Amst). 2009;8:1390-9 pubmed publisher
    ..From these data we conclude that an evolutionary conserved Mms1-Mms22 complex is required for replication of damaged DNA in fission yeast. ..
  11. Holmberg C, Fleck O, Hansen H, Liu C, Slaaby R, Carr A, et al. Ddb1 controls genome stability and meiosis in fission yeast. Genes Dev. 2005;19:853-62 pubmed
    ..These results suggest that Ddb1, along with Cullin 4 and the signalosome, constitute a major pathway controlling genome stability, repair, and differentiation via RNR regulation. ..
  12. Saitoh S, Chabes A, McDonald W, Thelander L, Yates J, Russell P. Cid13 is a cytoplasmic poly(A) polymerase that regulates ribonucleotide reductase mRNA. Cell. 2002;109:563-73 pubmed
    ..cid13 mutants have reduced dNTP pools and are sensitive to hydroxyurea, an RNR inhibitor. We propose that Cid13 defines a cytoplasmic form of poly(A) polymerase important for DNA replication and genome maintenance. ..
  13. Xu Y, Kelly T. Autoinhibition and autoactivation of the DNA replication checkpoint kinase Cds1. J Biol Chem. 2009;284:16016-27 pubmed publisher
    b>Cds1 is the ortholog of Chk2 and the major effector of the DNA replication checkpoint in Schizosaccharomyces pombe. Previous studies have shown that Cds1 is activated by a two-stage mechanism...
  14. Meister P, Taddei A, Ponti A, Baldacci G, Gasser S. Replication foci dynamics: replication patterns are modulated by S-phase checkpoint kinases in fission yeast. EMBO J. 2007;26:1315-26 pubmed
    ..In cells lacking the intra-S checkpoint kinase Cds1, replication factories dismantle on HU...
  15. Sabatinos S, Green M, Forsburg S. Continued DNA synthesis in replication checkpoint mutants leads to fork collapse. Mol Cell Biol. 2012;32:4986-97 pubmed publisher
    Hydroxyurea (HU) treatment activates the intra-S phase checkpoint proteins Cds1 and Mrc1 to prevent replication fork collapse...
  16. Ansbach A, Noguchi C, Klansek I, Heidlebaugh M, Nakamura T, Noguchi E. RFCCtf18 and the Swi1-Swi3 complex function in separate and redundant pathways required for the stabilization of replication forks to facilitate sister chromatid cohesion in Schizosaccharomyces pombe. Mol Biol Cell. 2008;19:595-607 pubmed
    ..Loss of Ctf18 leads to an increased sensitivity to S phase stressing agents, a decreased level of Cds1 kinase activity, and accumulation of DNA damage during S phase...
  17. Taricani L, Wang T. Rad4TopBP1, a scaffold protein, plays separate roles in DNA damage and replication checkpoints and DNA replication. Mol Biol Cell. 2006;17:3456-68 pubmed
  18. Yoshida S, Al Amodi H, Nakamura T, McInerny C, Shimoda C. The Schizosaccharomyces pombe cdt2(+) gene, a target of G1-S phase-specific transcription factor complex DSC1, is required for mitotic and premeiotic DNA replication. Genetics. 2003;164:881-93 pubmed
    ..These observations suggest that cdt2(+) plays an important role in DNA replication in both the mitotic and the meiotic life cycles of fission yeast. ..
  19. Nilssen E, Synnes M, Kleckner N, Grallert B, Boye E. Intra-G1 arrest in response to UV irradiation in fission yeast. Proc Natl Acad Sci U S A. 2003;100:10758-63 pubmed
    ..not have the molecular hallmarks of known checkpoint responses: it is independent of the checkpoint proteins Rad3, Cds1, and Chk1 and does not elicit inhibitory phosphorylation of Cdc2...
  20. Moser B, Brondello J, Baber Furnari B, Russell P. Mechanism of caffeine-induced checkpoint override in fission yeast. Mol Cell Biol. 2000;20:4288-94 pubmed
    ..The molecular target of caffeine was sought in fission yeast. Caffeine prevented activation of Cds1 and phosphorylation of Chk1, two protein kinases that enforce the S-M checkpoint triggered by hydroxyurea...
  21. Matsumoto S, Shimmoto M, Kakusho N, Yokoyama M, Kanoh Y, Hayano M, et al. Hsk1 kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeast. Cell Cycle. 2010;9:4627-37 pubmed
    In fission yeast, replication fork arrest activates the replication checkpoint effector kinase Cds1(Chk2/Rad53) through the Rad3(ATR/Mec1)-Mrc1(Claspin) pathway...
  22. Hu J, Sun L, Shen F, Chen Y, Hua Y, Liu Y, et al. The intra-S phase checkpoint targets Dna2 to prevent stalled replication forks from reversing. Cell. 2012;149:1221-32 pubmed publisher
    ..2001; Sogo et al., 2002; Tercero and Diffley, 2001). Using fission yeast, we report that the Cds1(Chk2) effector kinase targets Dna2 on S220 to regulate, both in vivo and in vitro, Dna2 association with stalled ..
  23. Liang D, Forsburg S. Characterization of Schizosaccharomyces pombe mcm7(+) and cdc23(+) (MCM10) and interactions with replication checkpoints. Genetics. 2001;159:471-86 pubmed
    ..Mcm7p is a nuclear protein throughout the cell cycle, and its localization is dependent on the other MCM proteins. Our data suggest that the Mcm3p-Mcm5p dimer interacts with the Mcm4p-Mcm6p-Mcm7p core complex through Mcm7p. ..
  24. Dolan W, Le A, Schmidt H, Yuan J, Green M, Forsburg S. Fission yeast Hsk1 (Cdc7) kinase is required after replication initiation for induced mutagenesis and proper response to DNA alkylation damage. Genetics. 2010;185:39-53 pubmed publisher
    ..We conclude that Hsk1-Dfp1 kinase functions post-initiation to maintain replication fork stability, an activity potentially mediated by the C terminus of Dfp1. ..
  25. Janes S, Schmidt U, Ashour Garrido K, Ney N, Concilio S, Zekri M, et al. Heat induction of a novel Rad9 variant from a cryptic translation initiation site reduces mitotic commitment. J Cell Sci. 2012;125:4487-97 pubmed publisher
    ..Taken together, heat reprogrammes the DNA damage checkpoint at the level of Chk1 by inducing a Rad9 variant that can act outside of the canonical 9-1-1 complex. ..
  26. Noguchi E, Noguchi C, Du L, Russell P. Swi1 prevents replication fork collapse and controls checkpoint kinase Cds1. Mol Cell Biol. 2003;23:7861-74 pubmed
    ..Our studies have shown that Swi1 is vital for proficient activation of the Rad53-like checkpoint kinase Cds1. Together they are required to prevent fork collapse in the ribosomal DNA repeats, and they also prevent ..
  27. Gómez Escoda B, Ivanova T, Calvo I, Alves Rodrigues I, Hidalgo E, Ayte J. Yox1 links MBF-dependent transcription to completion of DNA synthesis. EMBO Rep. 2011;12:84-9 pubmed publisher
    ..In fission yeast, Cds1 is the effector kinase of this checkpoint, inhibiting M-phase entry, stabilizing stalled replication forks and ..
  28. Boddy M, Lopez Girona A, Shanahan P, Interthal H, Heyer W, Russell P. Damage tolerance protein Mus81 associates with the FHA1 domain of checkpoint kinase Cds1. Mol Cell Biol. 2000;20:8758-66 pubmed
    b>Cds1, a serine/threonine kinase, enforces the S-M checkpoint in the fission yeast Schizosaccharomyces pombe...
  29. Lindsay H, Griffiths D, Edwards R, Christensen P, Murray J, Osman F, et al. S-phase-specific activation of Cds1 kinase defines a subpathway of the checkpoint response in Schizosaccharomyces pombe. Genes Dev. 1998;12:382-95 pubmed
    ..Here, we show that the Cds1 kinase is required to slow S phase in the presence of DNA-damaging agents...
  30. Furuya K, Poitelea M, Guo L, Caspari T, Carr A. Chk1 activation requires Rad9 S/TQ-site phosphorylation to promote association with C-terminal BRCT domains of Rad4TOPBP1. Genes Dev. 2004;18:1154-64 pubmed
    ..Rad9-Rad4(TOPBP1) interaction is required to activate the Chk1 damage checkpoint but not the Cds1 replication checkpoint...
  31. Limbo O, Porter Goff M, Rhind N, Russell P. Mre11 nuclease activity and Ctp1 regulate Chk1 activation by Rad3ATR and Tel1ATM checkpoint kinases at double-strand breaks. Mol Cell Biol. 2011;31:573-83 pubmed publisher
  32. Pankratz D, Forsburg S. Meiotic S-phase damage activates recombination without checkpoint arrest. Mol Biol Cell. 2005;16:1651-60 pubmed
    ..Our results suggest that fission yeast meiosis is exceptionally tolerant of DNA damage, and that some forms of spontaneous S-phase damage can be repaired by recombination without activating checkpoint arrest. ..
  33. Murakami H, Okayama H. A kinase from fission yeast responsible for blocking mitosis in S phase. Nature. 1995;374:817-9 pubmed
    ..This gene, named cds1+ (checking DNA synthesis), encodes a typical protein kinase...
  34. Bailis J, Luche D, Hunter T, Forsburg S. Minichromosome maintenance proteins interact with checkpoint and recombination proteins to promote s-phase genome stability. Mol Cell Biol. 2008;28:1724-38 pubmed publisher
    ..In cells where the checkpoint is activated, Mcm4 binds the Cds1 kinase and undergoes Cds1-dependent phosphorylation...
  35. Caetano C, Klier S, de Bruin R. Phosphorylation of the MBF repressor Yox1p by the DNA replication checkpoint keeps the G1/S cell-cycle transcriptional program active. PLoS ONE. 2011;6:e17211 pubmed publisher
    ..The remodeling of the cell cycle transcriptional program by the DNA replication checkpoint is likely to comprise an important mechanism for the avoidance of genomic instability. ..
  36. Ogino K, Masai H. Rad3-Cds1 mediates coupling of initiation of meiotic recombination with DNA replication. Mei4-dependent transcription as a potential target of meiotic checkpoint. J Biol Chem. 2006;281:1338-44 pubmed
    ..Examination of the roles of downstream checkpoint kinases reveals that Cds1, but not Chk1 or Mek1, is required for suppression of DSB in the presence of hydroxyurea...
  37. Kai M, Boddy M, Russell P, Wang T. Replication checkpoint kinase Cds1 regulates Mus81 to preserve genome integrity during replication stress. Genes Dev. 2005;19:919-32 pubmed
    The replication checkpoint kinase Cds1 preserves genome integrity by stabilizing stalled replication forks. Cds1 targets substrates through its FHA domain...
  38. Zeng Y, Piwnica Worms H. DNA damage and replication checkpoints in fission yeast require nuclear exclusion of the Cdc25 phosphatase via 14-3-3 binding. Mol Cell Biol. 1999;19:7410-9 pubmed
    ..These results indicate that 14-3-3 binding contributes to the nuclear exclusion of Cdc25 and that the nuclear exclusion of Cdc25 is required for a normal checkpoint response to both damaged and unreplicated DNA. ..
  39. Takeda T, Ogino K, Tatebayashi K, Ikeda H, Arai Ki -, Masai H. Regulation of initiation of S phase, replication checkpoint signaling, and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast. Mol Biol Cell. 2001;12:1257-74 pubmed
    ..maintenance of proper chromatin structures during mitotic S phase through regulating the functions of Rad3 (ATM)-Cds1 and Rad21 (cohesin), respectively, in addition to expected essential roles for initiation of mitotic DNA ..
  40. Tanaka K, Russell P. Mrc1 channels the DNA replication arrest signal to checkpoint kinase Cds1. Nat Cell Biol. 2001;3:966-72 pubmed
    ..gene in fission yeast, mrc1 (mediator of replication checkpoint), that confers activation of the checkpoint kinase Cds1 to DNA synthesis (S) phase...
  41. Grande M, Lambea E, Fajardo A, López Avilés S, Kellogg D, Aligue R. Crosstalk between Nap1 protein and Cds1 checkpoint kinase to maintain chromatin integrity. Biochim Biophys Acta. 2008;1783:1595-604 pubmed publisher
    ..cells are viable but they showed a delay in the onset of mitosis which is rescued by the absence of the replication Cds1 checkpoint kinase. In contrast, the absence of the DNA-damage Chk1 checkpoint kinase is unable to rescue the delay...
  42. Yue M, Zeng L, Singh A, Xu Y. Rad4 mainly functions in Chk1-mediated DNA damage checkpoint pathway as a scaffold protein in the fission yeast Schizosaccharomyces pombe. PLoS ONE. 2014;9:e92936 pubmed publisher
    ..with those from the biochemical studies, show that Rad4 mainly functions as a scaffold protein in the Chk1, not the Cds1(CHK2 in humans), checkpoint pathway...
  43. Forbes K, Humphrey T, Enoch T. Suppressors of cdc25p overexpression identify two pathways that influence the G2/M checkpoint in fission yeast. Genetics. 1998;150:1361-75 pubmed
    ..These results suggest that chk1(+) and rad24(+) may function redundantly with cds1(+) in the checkpoint response to unreplicated DNA.
  44. Haldar D, Kamakaka R. Schizosaccharomyces pombe Hst4 functions in DNA damage response by regulating histone H3 K56 acetylation. Eukaryot Cell. 2008;7:800-13 pubmed publisher
    ..We propose that Hst4 is a deacetylase involved in the restoration of chromatin structure following the S phase of cell cycle and DNA damage response. ..
  45. Nitani N, Yadani C, Yabuuchi H, Masukata H, Nakagawa T. Mcm4 C-terminal domain of MCM helicase prevents excessive formation of single-stranded DNA at stalled replication forks. Proc Natl Acad Sci U S A. 2008;105:12973-8 pubmed publisher
    ..These results suggest that the Mcm4 CTD is required to suspend MCM-helicase activity after the formation of single-stranded DNA sufficient for checkpoint activation. ..
  46. Schonbrun M, Laor D, López Maury L, Bahler J, Kupiec M, Weisman R. TOR complex 2 controls gene silencing, telomere length maintenance, and survival under DNA-damaging conditions. Mol Cell Biol. 2009;29:4584-94 pubmed publisher
    ..These data place TOR proteins in line with other PIKK members, such as ATM and ATR, as guardians of genome stability. ..
  47. Miyabe I, Morishita T, Shinagawa H, Carr A. Schizosaccharomyces pombe Cds1Chk2 regulates homologous recombination at stalled replication forks through the phosphorylation of recombination protein Rad60. J Cell Sci. 2009;122:3638-43 pubmed publisher
    ..Rad60 is phosphorylated in response to hydroxyurea (HU)-induced DNA replication arrest in a Cds1(Chk2)-dependent manner...
  48. Xu Y. Inner nuclear membrane protein Lem2 facilitates Rad3-mediated checkpoint signaling under replication stress induced by nucleotide depletion in fission yeast. Cell Signal. 2016;28:235-45 pubmed publisher
    ..which works cooperatively with mediator Mrc1 and the 9-1-1 checkpoint clamp to phosphorylate the effector kinase Cds1 (CHK2/Rad53). Phosphorylation of Cds1 promotes autoactivation of the kinase...
  49. Shimada M, Nabeshima K, Tougan T, Nojima H. The meiotic recombination checkpoint is regulated by checkpoint rad+ genes in fission yeast. EMBO J. 2002;21:2807-18 pubmed
    ..We also found that the delay in entering nuclear division was dependent on the checkpoint rad+, cds1+ and mek1+ (the meiotic paralog of Cds1/Chk2)...
  50. Kume K, Koyano T, Kanai M, Toda T, Hirata D. Calcineurin ensures a link between the DNA replication checkpoint and microtubule-dependent polarized growth. Nat Cell Biol. 2011;13:234-42 pubmed publisher
    ..Here we show that the fission yeast checkpoint kinase Cds1 (Chk2 homologue) delays the transition of growth polarity from monopolar to bipolar (termed NETO; new-end take-off)...
  51. Francesconi S, Smeets M, Grenon M, Tillit J, Blaisonneau J, Baldacci G. Fission yeast chk1 mutants show distinct responses to different types of DNA damaging treatments. Genes Cells. 2002;7:663-73 pubmed
    ..We show that both mutant proteins are not phosphorylated in cds1 devoid cells treated with ribonucleotide reductase inhibitor hydroxyurea or when the replisome is affected by a ..
  52. Akai Y, Kurokawa Y, Nakazawa N, Tonami Murakami Y, Suzuki Y, Yoshimura S, et al. Opposing role of condensin hinge against replication protein A in mitosis and interphase through promoting DNA annealing. Open Biol. 2011;1:110023 pubmed publisher
    ..We show that 16 suppressor mutations of cut14-Y1 were all mapped within the hinge domain, which surrounded the original L543 mutation site. ..
  53. Tan S, Wang T. Analysis of fission yeast primase defines the checkpoint responses to aberrant S phase initiation. Mol Cell Biol. 2000;20:7853-66 pubmed
    ..defect also has a higher extent of Polalpha-primase complex instability and deficiency in the hydroxyurea-induced Cds1-mediated intra-S phase checkpoint response...
  54. Lambert S, Mason S, Barber L, Hartley J, Pearce J, Carr A, et al. Schizosaccharomyces pombe checkpoint response to DNA interstrand cross-links. Mol Cell Biol. 2003;23:4728-37 pubmed
    ..Only a transient activation of the Cds1 DNA replication checkpoint factor occurs following ICL formation in wild-type cells, but this is increased and ..
  55. Williams D, McIntosh J. Mcl1p is a polymerase alpha replication accessory factor important for S-phase DNA damage survival. Eukaryot Cell. 2005;4:166-77 pubmed
    ..Further, we find that the overexpression of the S-phase checkpoint kinase, Cds1, or the loss of Hsk1 kinase activity can disrupt Mcl1p's interaction with chromatin and Pol1p during replication ..
  56. Ding L, Forsburg S. Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response. G3 (Bethesda). 2014;4:1373-84 pubmed publisher
  57. Sartagul W, Zhou X, Yamada Y, Ma N, Tanaka K, Furuyashiki T, et al. The MluI cell cycle box (MCB) motifs, but not damage-responsive elements (DREs), are responsible for the transcriptional induction of the rhp51+ gene in response to DNA replication stress. PLoS ONE. 2014;9:e111936 pubmed publisher
    ..Using cells deficient in checkpoint signaling molecules, we found that the Rad3-Cds1/Chk1 pathway partially mediated rhp51+ transcription in response to DNA replication stress, suggesting the ..
  58. Ruan K, Yamamoto T, Asakawa H, Chikashige Y, Masukata H, Haraguchi T, et al. Meiotic nuclear movements in fission yeast are regulated by the transcription factor Mei4 downstream of a Cds1-dependent replication checkpoint pathway. Genes Cells. 2015;20:160-72 pubmed publisher
    ..We found that this prolongation was caused by the Cds1-dependent replication checkpoint, which represses expression of the mei4(+) gene encoding a meiosis-specific ..
  59. Santosa V, Martha S, Hirose N, Tanaka K. The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication. J Biol Chem. 2013;288:6864-80 pubmed publisher
    ..These results strongly suggest that Mcb1 promotes efficient pre-RC formation during DNA replication by regulating the MCM complex...
  60. Nilssen E, Synnes M, Tvegård T, Vebø H, Boye E, Grallert B. Germinating fission yeast spores delay in G1 in response to UV irradiation. BMC Cell Biol. 2004;5:40 pubmed
    ..The first takes place before entry into S phase, does not depend on the checkpoint proteins Rad3, Cds1 and Chk1 and is independent of Cdc2 phosphorylation...
  61. Kunoh T, Habu T, Matsumoto T. Involvement of fission yeast Clr6-HDAC in regulation of the checkpoint kinase Cds1. Nucleic Acids Res. 2008;36:3311-9 pubmed publisher
    ..Here, we show that fission yeast Clr6-HDAC (histone deacetylase) regulates the checkpoint kinase Cds1 when DNA replication encounters a stressful condition...
  62. Snaith H, Marlett J, Forsburg S. Ibp1p, a novel Cdc25-related phosphatase, suppresses Schizosaccharomyces pombe hsk1 ( cdc7). Curr Genet. 2003;44:38-48 pubmed
    ..Ibp1 is not required for viability, suggesting it may be a non-essential regulator of DNA replication or chromosome structure during S phase. ..
  63. Iyer D, Rhind N. Replication fork slowing and stalling are distinct, checkpoint-independent consequences of replicating damaged DNA. PLoS Genet. 2017;13:e1006958 pubmed publisher
    ..Finally, using a novel analytic approach, we rigorously identify fork stalling events in our combing data and show that they play a previously unappreciated role in shaping replication kinetics in response to DNA damage. ..