Gene Symbol: cdc8
Description: tropomyosin
Alias: fus4, tropomyosin
Species: fission yeast
Products:     cdc8

Top Publications

  1. Chang F, Drubin D, Nurse P. cdc12p, a protein required for cytokinesis in fission yeast, is a component of the cell division ring and interacts with profilin. J Cell Biol. 1997;137:169-82 pubmed
    ..cdc12p localization is altered in actin ring mutants. cdc8 (tropomyosin homologue), cdc3 (profilin homologue), and cdc15 mutants exhibit no specific cdc12p staining during mitosis...
  2. Balasubramanian M, McCollum D, Chang L, Wong K, Naqvi N, He X, et al. Isolation and characterization of new fission yeast cytokinesis mutants. Genetics. 1998;149:1265-75 pubmed
    ..In addition, we have characterized a phenotype that has not been described previously in cytokinesis mutants, namely the failure to reorganize actin patches to the medial region of the cell in preparation for septum formation. ..
  3. Clayton J, Pollard L, Sckolnick M, Bookwalter C, Hodges A, Trybus K, et al. Fission yeast tropomyosin specifies directed transport of myosin-V along actin cables. Mol Biol Cell. 2014;25:66-75 pubmed publisher
    ..however, that the fission yeast myosin-V (Myo52p) is a nonprocessive motor whose activity is enhanced by tropomyosin (Cdc8p)...
  4. Motegi F, Nakano K, Kitayama C, Yamamoto M, Mabuchi I. Identification of Myo3, a second type-II myosin heavy chain in the fission yeast Schizosaccharomyces pombe. FEBS Lett. 1997;420:161-6 pubmed
    ..Double mutant analysis indicated that Myo3 genetically interacts with Cdc8 tropomyosin and actin. Myo3 may be implicated in cytokinesis and stabilization of F-actin cables...
  5. Skoumpla K, Coulton A, Lehman W, Geeves M, Mulvihill D. Acetylation regulates tropomyosin function in the fission yeast Schizosaccharomyces pombe. J Cell Sci. 2007;120:1635-45 pubmed
    b>Tropomyosin is an evolutionarily conserved alpha-helical coiled-coil protein that promotes and maintains actin filaments...
  6. Chang F, Woollard A, Nurse P. Isolation and characterization of fission yeast mutants defective in the assembly and placement of the contractile actin ring. J Cell Sci. 1996;109 ( Pt 1):131-42 pubmed
    ..Mutants of cdc3 and cdc8, which encode profilin and tropomyosin respectively, display disorganized actin patches in all cells...
  7. East D, Sousa D, Martin S, Edwards T, Lehman W, Mulvihill D. Altering the stability of the Cdc8 overlap region modulates the ability of this tropomyosin to bind co-operatively to actin and regulate myosin. Biochem J. 2011;438:265-73 pubmed publisher
    Tm (tropomyosin) is an evolutionarily conserved ?-helical coiled-coil protein, dimers of which form end-to-end polymers capable of associating with and stabilizing actin filaments, and regulating myosin function...
  8. Coulton A, East D, Galinska Rakoczy A, Lehman W, Mulvihill D. The recruitment of acetylated and unacetylated tropomyosin to distinct actin polymers permits the discrete regulation of specific myosins in fission yeast. J Cell Sci. 2010;123:3235-43 pubmed publisher
    b>Tropomyosin (Tm) is a conserved dimeric coiled-coil protein, which forms polymers that curl around actin filaments in order to regulate actomyosin function...
  9. Clayton J, Sammons M, Stark B, Hodges A, Lord M. Differential regulation of unconventional fission yeast myosins via the actin track. Curr Biol. 2010;20:1423-31 pubmed publisher
    ..However, decoration of actin with tropomyosin differentially regulates the activity of these motors...

More Information


  1. Stark B, Sladewski T, Pollard L, Lord M. Tropomyosin and myosin-II cellular levels promote actomyosin ring assembly in fission yeast. Mol Biol Cell. 2010;21:989-1000 pubmed publisher
    Myosin-II (Myo2p) and tropomyosin are essential for contractile ring formation and cytokinesis in fission yeast. Here we used a combination of in vivo and in vitro approaches to understand how these proteins function at contractile rings...
  2. Wu J, Kuhn J, Kovar D, Pollard T. Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis. Dev Cell. 2003;5:723-34 pubmed
    ..10 min prior to anaphase B, this broad band of proteins condenses into a contractile ring including actin, tropomyosin (Cdc8p), and alpha-actinin (Ain1p)...
  3. Balasubramanian M, Helfman D, Hemmingsen S. A new tropomyosin essential for cytokinesis in the fission yeast S. pombe. Nature. 1992;360:84-7 pubmed
    ..Here we clone cdc8+ and find that it encodes a novel tropomyosin. Gene disruption results in lethal arrest of the cell cycle, but spore germination, cell growth, DNA ..
  4. Arai R, Nakano K, Mabuchi I. Subcellular localization and possible function of actin, tropomyosin and actin-related protein 3 (Arp3) in the fission yeast Schizosaccharomyces pombe. Eur J Cell Biol. 1998;76:288-95 pubmed
    ..subcellular localizations and interactions of actin and two actin cytoskeleton-related proteins, Cdc8 tropomyosin and actin-related protein 3, Arp3, in the fission yeast Schizosaccharomyces pombe, using specific antibodies ..
  5. Kurahashi H, Imai Y, Yamamoto M. Tropomyosin is required for the cell fusion process during conjugation in fission yeast. Genes Cells. 2002;7:375-84 pubmed
    b>Tropomyosin is an actin-binding protein, which is thought to stabilize actin filaments and influence many aspects of F-actin...
  6. Le Goff X, Woollard A, Simanis V. Analysis of the cps1 gene provides evidence for a septation checkpoint in Schizosaccharomyces pombe. Mol Gen Genet. 1999;262:163-72 pubmed
    ..Analysis of the interaction of cps1 with other mutations suggests the existence of a septation checkpoint, which requires the activity of the protein kinase weel for function. ..
  7. Skau C, Neidt E, Kovar D. Role of tropomyosin in formin-mediated contractile ring assembly in fission yeast. Mol Biol Cell. 2009;20:2160-73 pubmed publisher
    ..In addition to formin Cdc12p and profilin, the single tropomyosin isoform SpTm is required for contractile ring assembly...
  8. Hou M, Wiley D, Verde F, McCollum D. Mob2p interacts with the protein kinase Orb6p to promote coordination of cell polarity with cell cycle progression. J Cell Sci. 2003;116:125-35 pubmed
    ..Our results suggest that Mob2p, along with Orb6p, is required for coordinating polarized cell growth during interphase with the onset of mitosis. ..
  9. Rupes I, Jia Z, Young P. Ssp1 promotes actin depolymerization and is involved in stress response and new end take-off control in fission yeast. Mol Biol Cell. 1999;10:1495-510 pubmed
    ..Loss of Ssp1 function inhibits actin relocalization during osmotic stress, in cdc3 and cdc8 mutant backgrounds, and in the presence of latrunculin A, implicating Ssp1 in promotion of actin depolymerization...
  10. Cranz Mileva S, Pamula M, Barua B, Desai B, Hong Y, Russell J, et al. A molecular evolution approach to study the roles of tropomyosin in fission yeast. PLoS ONE. 2013;8:e76726 pubmed publisher
    b>Tropomyosin, a coiled-coil protein that binds along the length of the actin filament, is a universal regulator of the actin cytoskeleton...
  11. Subramanian D, Huang J, Sevugan M, Robinson R, Balasubramanian M, Tang X. Insight into actin organization and function in cytokinesis from analysis of fission yeast mutants. Genetics. 2013;194:435-46 pubmed publisher
    ..We also characterize a mutant allele of actin that is suppressed upon overexpression of Cdc8p-tropomyosin, underscoring the utility of this mutant bank...
  12. Goss J, Kim S, Bledsoe H, Pollard T. Characterization of the roles of Blt1p in fission yeast cytokinesis. Mol Biol Cell. 2014;25:1946-57 pubmed publisher
    ..We find three defects that likely contribute to this delay. Without Blt1p, contractile rings recruited and retained less Sid2p/Mob1p and Clp1p phosphatase, and ?-glucan synthase Bgs1p accumulated slowly at the cleavage site. ..
  13. Demeter J, Sazer S. imp2, a new component of the actin ring in the fission yeast Schizosaccharomyces pombe. J Cell Biol. 1998;143:415-27 pubmed
    ..imp2 also shows genetic interactions with several, previously characterized septation genes, strengthening the conclusion that it plays a role in normal fission yeast septation. ..
  14. Brooker H, Geeves M, Mulvihill D. Analysis of biophysical and functional consequences of tropomyosin-fluorescent protein fusions. FEBS Lett. 2016;590:3111-21 pubmed publisher
    ..These dynamic properties are determined by different isoforms of tropomyosin which are recruited to distinct subpopulations of actin polymers to differentially regulate their functional ..
  15. Christensen J, Hocky G, Homa K, Morganthaler A, Hitchcock Degregori S, Voth G, et al. Competition between Tropomyosin, Fimbrin, and ADF/Cofilin drives their sorting to distinct actin filament networks. elife. 2017;6: pubmed publisher
    ..By focusing on the stabilizing protein tropomyosin Cdc8, bundling protein fimbrin Fim1, and severing protein coffin Adf1, we examined how their pairwise and ..
  16. Cranz Mileva S, MacTaggart B, Russell J, Hitchcock Degregori S. Evolutionarily conserved sites in yeast tropomyosin function in cell polarity, transport and contractile ring formation. Biol Open. 2015;4:1040-51 pubmed publisher
    b>Tropomyosin is a coiled-coil protein that binds and regulates actin filaments. The tropomyosin gene in Schizosaccharomyces pombe, cdc8, is required for formation of actin cables, contractile rings, and polar localization of actin patches...
  17. Takaine M, Numata O, Nakano K. An actin-myosin-II interaction is involved in maintaining the contractile ring in fission yeast. J Cell Sci. 2015;128:2903-18 pubmed publisher
    ..We propose that large assemblies of myosin-II proteins consolidate the contractile ring by continuously binding to F-actin in the ring, thereby contributing to its maintenance. ..
  18. Skau C, Courson D, Bestul A, Winkelman J, Rock R, Sirotkin V, et al. Actin filament bundling by fimbrin is important for endocytosis, cytokinesis, and polarization in fission yeast. J Biol Chem. 2011;286:26964-77 pubmed publisher
    ..We previously discovered that a principal role of Fim1 is to control localization of tropomyosin Cdc8, thereby facilitating cofilin-mediated filament turnover...
  19. Dudin O, Merlini L, Bendezú F, Groux R, Vincenzetti V, Martin S. A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion. PLoS Genet. 2017;13:e1006721 pubmed publisher
    ..Rng8 and Rng9 accumulate on the fusion focus, dependent on Myo51 and tropomyosin Cdc8...
  20. Cullen C, May K, Hagan I, Glover D, Ohkura H. A new genetic method for isolating functionally interacting genes: high plo1(+)-dependent mutants and their suppressors define genes in mitotic and septation pathways in fission yeast. Genetics. 2000;155:1521-34 pubmed
    ..As multicopy suppressors, we isolated four known genes involved in septation in fission yeast: spg1(+), sce3(+), cdc8(+), and rho1(+), and two previously uncharacterized genes, mpd1(+) and mpd2(+)...
  21. Skau C, Kovar D. Fimbrin and tropomyosin competition regulates endocytosis and cytokinesis kinetics in fission yeast. Curr Biol. 2010;20:1415-22 pubmed publisher
    b>Tropomyosin is an important actin filament-stabilizing protein that controls the access of other essential proteins to filaments, including myosin motors, Arp2/3 complex, formin, and cofilin...
  22. McCollum D, Balasubramanian M, Gould K. Identification of cold-sensitive mutations in the Schizosaccharomyces pombe actin locus. FEBS Lett. 1999;451:321-6 pubmed
    ..pombe. ..
  23. Salus S, Demeter J, Sazer S. The Ran GTPase system in fission yeast affects microtubules and cytokinesis in cells that are competent for nucleocytoplasmic protein transport. Mol Cell Biol. 2002;22:8491-505 pubmed
  24. Bahler J, Pringle J. Pom1p, a fission yeast protein kinase that provides positional information for both polarized growth and cytokinesis. Genes Dev. 1998;12:1356-70 pubmed
    ..Thus, Pom1p may position the growth and cytokinesis machineries by interaction with both the actin and microtubule cytoskeletons. ..
  25. Dudin O, Bendezú F, Groux R, Laroche T, Seitz A, Martin S. A formin-nucleated actin aster concentrates cell wall hydrolases for cell fusion in fission yeast. J Cell Biol. 2015;208:897-911 pubmed publisher
    ..Thus, the fusion focus focalizes cell wall dissolution within a broader cell wall synthesis zone to shift from cell growth to cell fusion. ..
  26. Pelham R, Chang F. Role of actin polymerization and actin cables in actin-patch movement in Schizosaccharomyces pombe. Nat Cell Biol. 2001;3:235-44 pubmed
    ..In a cdc8 (tropomyosin) mutant, actin cables are absent, and patches move with similar speed but in a non-directed manner...