cdc2

Summary

Gene Symbol: cdc2
Description: cyclin-dependent protein kinase Cdk1/Cdc2
Alias: cdk1, swo2, tws1, cyclin-dependent protein kinase Cdk1/Cdc2
Species: fission yeast

Top Publications

  1. Caspari T, Murray J, Carr A. Cdc2-cyclin B kinase activity links Crb2 and Rqh1-topoisomerase III. Genes Dev. 2002;16:1195-208 pubmed
    ..Here we provide evidence that the major Schizosaccharomyces pombe CDK, Cdc2-cyclin B, influences recombinational repair of radiation-induced DSBs during the G(2) phase at two distinct stages...
  2. Barbet N, Carr A. Fission yeast wee1 protein kinase is not required for DNA damage-dependent mitotic arrest. Nature. 1993;364:824-7 pubmed
    ..The activity of p34cdc2 is controlled, in part, by the wee1 protein kinase, which inactivates cdc2 through phosphorylation at tyrosine-15 (ref. 7). Here we report normal mitotic arrest after DNA damage in S...
  3. Aoki K, Nakaseko Y, Kinoshita K, Goshima G, Yanagida M. CDC2 phosphorylation of the fission yeast dis1 ensures accurate chromosome segregation. Curr Biol. 2006;16:1627-35 pubmed
    ..Here, we report that Dis1is regulated by Cdc2 phosphorylation and that this mitotic phosphorylation ensures the fidelity of chromosome segregation...
  4. Liu J, Wang H, Balasubramanian M. A checkpoint that monitors cytokinesis in Schizosaccharomyces pombe. J Cell Sci. 2000;113 ( Pt 7):1223-30 pubmed
    ..G(2)/M transition defect of cps1-191 mutants is suppressed by a mutation in the wee1 gene and also by the dominant cdc2 allele cdc2-1w, but not the cdc2-3w allele...
  5. Booher R, Beach D. Interaction between cdc13+ and cdc2+ in the control of mitosis in fission yeast; dissociation of the G1 and G2 roles of the cdc2+ protein kinase. EMBO J. 1987;6:3441-7 pubmed
    A cold-sensitive (cs) allele of cdc2, a gene that acts in both the G1 and G2 phases of the fission yeast cell cycle, has been isolated by classical mutagenesis...
  6. Lee M, Enoch T, Piwnica Worms H. mik1+ encodes a tyrosine kinase that phosphorylates p34cdc2 on tyrosine 15. J Biol Chem. 1994;269:30530-7 pubmed
    ..The tyrosine 15 kinase activity co-eluted with the 68 kDa form of Mik1. These results indicate that mik1+ encodes a tyrosine kinase that directly phosphorylates p34cdc2 on tyrosine 15. ..
  7. Rumpf C, Cipak L, Schleiffer A, Pidoux A, Mechtler K, Tolić Nørrelykke I, et al. Laser microsurgery provides evidence for merotelic kinetochore attachments in fission yeast cells lacking Pcs1 or Clr4. Cell Cycle. 2010;9:3997-4004 pubmed
    ..We further show that Mde4 is regulated by Cdc2 and that Cdc2 activity prevents precocious localization of Mde4 to the metaphase spindle...
  8. Furnari B, Rhind N, Russell P. Cdc25 mitotic inducer targeted by chk1 DNA damage checkpoint kinase. Science. 1997;277:1495-7 pubmed
    ..of the cell cycle at the G2 checkpoint, induced by DNA damage, requires inhibitory phosphorylation of the kinase Cdc2 in both fission yeast and human cells...
  9. Kominami K, Toda T. Fission yeast WD-repeat protein pop1 regulates genome ploidy through ubiquitin-proteasome-mediated degradation of the CDK inhibitor Rum1 and the S-phase initiator Cdc18. Genes Dev. 1997;11:1548-60 pubmed
    In fission yeast, maintenance of genome ploidy is controlled by at least two mechanisms. One operates through the Cdc2/Cdc13 kinase, which also involves the CDK inhibitor Rum1, and the other through the S-phase regulator Cdc18...

More Information

Publications82

  1. Walworth N, Davey S, Beach D. Fission yeast chk1 protein kinase links the rad checkpoint pathway to cdc2. Nature. 1993;363:368-71 pubmed
    ..Identification of a gene involved in checkpoint control as a rescue of a cdc2 mutant links the rad1-dependent DNA-damage-sensing pathway and p34cdc2 activity.
  2. Nurse P, Thuriaux P, Nasmyth K. Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet. 1976;146:167-78 pubmed
    ..Cell plate formation itself is usually dependent upon the completion of nuclear division. ..
  3. Morrell J, Tomlin G, Rajagopalan S, Venkatram S, Feoktistova A, Tasto J, et al. Sid4p-Cdc11p assembles the septation initiation network and its regulators at the S. pombe SPB. Curr Biol. 2004;14:579-84 pubmed
    ..Our results suggest that the Sid4p-Cdc11p complex organizes a signaling hub on the SPB and that this hub coordinates cell and nuclear division. ..
  4. Dischinger S, Krapp A, Xie L, Paulson J, Simanis V. Chemical genetic analysis of the regulatory role of Cdc2p in the S. pombe septation initiation network. J Cell Sci. 2008;121:843-53 pubmed publisher
    ..To study the role of Cdc2p in greater detail, we generated a cdc2 allele that is sensitive to an inhibitory ATP analogue...
  5. Fu C, Ward J, Loiodice I, Velve Casquillas G, Nedelec F, Tran P. Phospho-regulated interaction between kinesin-6 Klp9p and microtubule bundler Ase1p promotes spindle elongation. Dev Cell. 2009;17:257-67 pubmed publisher
  6. Papadopoulou K, Chen J, Mead E, Feoktistova A, Petit C, Agarwal M, et al. Regulation of cell cycle-specific gene expression in fission yeast by the Cdc14p-like phosphatase Clp1p. J Cell Sci. 2010;123:4374-81 pubmed publisher
  7. Qu M, Rappas M, Wardlaw C, Garcia V, Ren J, Day M, et al. Phosphorylation-dependent assembly and coordination of the DNA damage checkpoint apparatus by Rad4(TopBP1). Mol Cell. 2013;51:723-736 pubmed publisher
    ..Finally, we show that the simultaneous interaction of a single Rad4(TopBP1) molecule with both Thr187 phosphorylation sites in a Crb2(53BP1) dimer is essential for establishing the DNA damage checkpoint. ..
  8. Moreno S, Hayles J, Nurse P. Regulation of p34cdc2 protein kinase during mitosis. Cell. 1989;58:361-72 pubmed
    ..Therefore, the cdc25+, cdc13+, and suc1+ gene products are important for regulating p34cdc2 protein kinase activity during entry into, progress through, and exit from mitosis. ..
  9. Obara Ishihara T, Okayama H. A B-type cyclin negatively regulates conjugation via interacting with cell cycle 'start' genes in fission yeast. EMBO J. 1994;13:1863-72 pubmed
    ..Thus, in S.pombe, a B-type cyclin, regulated by the newly identified cell cycle 'start' genes, plays a crucial role in the control of sexual development. ..
  10. Correa Bordes J, Gulli M, Nurse P. p25rum1 promotes proteolysis of the mitotic B-cyclin p56cdc13 during G1 of the fission yeast cell cycle. EMBO J. 1997;16:4657-64 pubmed
    ..As a consequence, at this point in the cell cycle p56cdc13 proteolysis is inhibited, leading to a rise of p56cdc13 levels in preparation for mitosis. ..
  11. Le Goff X, Woollard A, Simanis V. Analysis of the cps1 gene provides evidence for a septation checkpoint in Schizosaccharomyces pombe. Mol Gen Genet. 1999;262:163-72 pubmed
    ..Analysis of the interaction of cps1 with other mutations suggests the existence of a septation checkpoint, which requires the activity of the protein kinase weel for function. ..
  12. Yoon H, Feoktistova A, Chen J, Jennings J, Link A, Gould K. Role of Hcn1 and its phosphorylation in fission yeast anaphase-promoting complex/cyclosome function. J Biol Chem. 2006;281:32284-93 pubmed
    ..Moreover, Hcn1 is a phosphoprotein in vivo. Phosphorylation of Hcn1 occurs at a single Cdk1 site in vitro and in vivo...
  13. Wolfe B, McDonald W, Yates J, Gould K. Phospho-regulation of the Cdc14/Clp1 phosphatase delays late mitotic events in S. pombe. Dev Cell. 2006;11:423-30 pubmed
    In eukaryotes, exit from mitosis occurs through the inactivation of the Cdk1-cyclin B kinase complex and the reversal of its phosphorylation events...
  14. Cueille N, Salimova E, Esteban V, Blanco M, Moreno S, Bueno A, et al. Flp1, a fission yeast orthologue of the s. cerevisiae CDC14 gene, is not required for cyclin degradation or rum1p stabilisation at the end of mitosis. J Cell Sci. 2001;114:2649-64 pubmed
    ..In contrast to S. cerevisiae Cdc14p, flp1p is also present on the mitotic spindle and contractile ring. The potential roles of flp1p in cell cycle control are discussed. ..
  15. Wuarin J, Buck V, Nurse P, Millar J. Stable association of mitotic cyclin B/Cdc2 to replication origins prevents endoreduplication. Cell. 2002;111:419-31 pubmed
    We show that in fission yeast the mitotic B type cyclin Cdc13/Cdc2 kinase associates with replication origins in vivo...
  16. Choi S, Péli Gulli M, Mcleod I, Sarkeshik A, Yates J, Simanis V, et al. Phosphorylation state defines discrete roles for monopolin in chromosome attachment and spindle elongation. Curr Biol. 2009;19:985-95 pubmed publisher
    It is unknown how oscillations in Cdk1 activity drive the dramatic changes in chromosome and spindle dynamics that occur at the metaphase/anaphase transition...
  17. Fantes P. Isolation of cell size mutants of a fission yeast by a new selective method: characterization of mutants and implications for division control mechanisms. J Bacteriol. 1981;146:746-54 pubmed
    ..Two partial wee1 mutations of a new type were found among the revertants. Two new wee mutations mapping at the cdc2 gene (cdc2-w mutants) were also obtained...
  18. Esashi F, Yanagida M. Cdc2 phosphorylation of Crb2 is required for reestablishing cell cycle progression after the damage checkpoint. Mol Cell. 1999;4:167-74 pubmed
    ..which resembles budding yeast Rad9p and human BRCA1, promotes checkpoint by activating Chk1 kinase, which restrains Cdc2 activation...
  19. Booher R, Beach D. Involvement of cdc13+ in mitotic control in Schizosaccharomyces pombe: possible interaction of the gene product with microtubules. EMBO J. 1988;7:2321-7 pubmed
    Previous genetic studies have shown that the fission yeast cdc13+ gene product interacts closely with the cdc2+ protein kinase during mitosis. Here, we have cloned the cdc13+ gene from a S...
  20. Gould K, Nurse P. Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis. Nature. 1989;342:39-45 pubmed
    The cdc2+ protein kinase (pp34) is found to be phosphorylated on tyrosine as well as serine and threonine residues in exponentially growing Schizosaccharomyces pombe...
  21. Nurse P, Bissett Y. Gene required in G1 for commitment to cell cycle and in G2 for control of mitosis in fission yeast. Nature. 1981;292:558-60 pubmed
  22. Booher R, Alfa C, Hyams J, Beach D. The fission yeast cdc2/cdc13/suc1 protein kinase: regulation of catalytic activity and nuclear localization. Cell. 1989;58:485-97 pubmed
    The products of the cdc13+ and cdc2+ genes form a stable complex that displays protein kinase activity in vitro. p63cdc13 is a substrate of p34cdc2, the catalytic subunit of the kinase...
  23. Yamano H, Kitamura K, Kominami K, Lehmann A, Katayama S, Hunt T, et al. The spike of S phase cyclin Cig2 expression at the G1-S border in fission yeast requires both APC and SCF ubiquitin ligases. Mol Cell. 2000;6:1377-87 pubmed
    ..These data indicate that the APC/C and SCF control Cig2 levels differentially at different stages of the cell cycle. ..
  24. Hirano T, Funahashi S, Uemura T, Yanagida M. Isolation and characterization of Schizosaccharomyces pombe cutmutants that block nuclear division but not cytokinesis. EMBO J. 1986;5:2973-9 pubmed
    ..A novel mutant nuc1 is also described which shows a cytological phenotype similar to the double mutant of DNA topoisomerases I and II but contains normal levels of both DNA topoisomerase activities. ..
  25. Simanis V, Nurse P. The cell cycle control gene cdc2+ of fission yeast encodes a protein kinase potentially regulated by phosphorylation. Cell. 1986;45:261-8 pubmed
    The cdc2+ gene function has an important role in controlling the commitment of the fission yeast cell to the mitotic cycle and the timing of mitosis...
  26. Tatebe H, Yanagida M. Cut8, essential for anaphase, controls localization of 26S proteasome, facilitating destruction of cyclin and Cut2. Curr Biol. 2000;10:1329-38 pubmed
    ..Cut8 is a novel evolutionarily conserved heat-inducible regulator. It facilitates anaphase-promoting proteolysis by recruiting 26S proteasome to a functionally efficient nuclear location. ..
  27. Fujita I, Nishihara Y, Tanaka M, Tsujii H, Chikashige Y, Watanabe Y, et al. Telomere-nuclear envelope dissociation promoted by Rap1 phosphorylation ensures faithful chromosome segregation. Curr Biol. 2012;22:1932-7 pubmed publisher
    ..The phosphorylation of the telomere protein Rap1 during mitosis, primarily by Cdc2, impedes the interaction between Rap1 and Bqt4, a nuclear membrane protein, thereby inducing telomere dissociation ..
  28. Ng S, Anderson M, White S, McInerny C. mik1(+) G1-S transcription regulates mitotic entry in fission yeast. FEBS Lett. 2001;503:131-4 pubmed
    ..The mitotic delay in cdc10-C4 cells is bypassed by cdc2-1w, suggesting that mik1(+) acts directly on cdc2(+), with no checkpoint function involved...
  29. Tasto J, Carnahan R, McDonald W, Gould K. Vectors and gene targeting modules for tandem affinity purification in Schizosaccharomyces pombe. Yeast. 2001;18:657-62 pubmed
    ..Several known binding proteins co-purified with both Cdc2p-CTAP and N-TAP-Cdc2p, indicating the usefulness of these tags for the rapid purification of stable protein complexes from Sz. pombe. ..
  30. Alves Rodrigues I, Ferreira P, Moldón A, Vivancos A, Hidalgo E, Guigó R, et al. Spatiotemporal Control of Forkhead Binding to DNA Regulates the Meiotic Gene Expression Program. Cell Rep. 2016;14:885-895 pubmed publisher
    ..We propose that Fkh2 serves as a placeholder until the later appearance of Mei4 with a higher affinity for DNA that induces the expression of a subset of meiotic genes. ..
  31. Aoi Y, Kawashima S, Simanis V, Yamamoto M, Sato M. Optimization of the analogue-sensitive Cdc2/Cdk1 mutant by in vivo selection eliminates physiological limitations to its use in cell cycle analysis. Open Biol. 2014;4: pubmed publisher
    ..The analogue-sensitive mutant cdc2-as of fission yeast (Schizosaccharomyces pombe) is a powerful tool to study the cell cycle, but the strain displays ..
  32. Furnari B, Russell P, Leatherwood J. Pch1(+), a second essential C-type cyclin gene in Schizosaccharomyces pombe. J Biol Chem. 1997;272:12100-6 pubmed
    ..pombe gene pch1(+) (pombe cyclin C homology) was isolated in a two-hybrid screen for proteins that interact with Cdc2. The cyclin box region of Pch1 protein shares greatest sequence identity with mammalian and Drosophila C-type ..
  33. Matsumoto T. A fission yeast homolog of CDC20/p55CDC/Fizzy is required for recovery from DNA damage and genetically interacts with p34cdc2. Mol Cell Biol. 1997;17:742-50 pubmed
    ..This defect is suppressed when slp1-362 is combined with wee1-50 or cdc2-3w; in these mutants, the onset of mitosis is advanced due to the premature activation of p34cdc2...
  34. Yamaguchi S, Okayama H, Nurse P. Fission yeast Fizzy-related protein srw1p is a G(1)-specific promoter of mitotic cyclin B degradation. EMBO J. 2000;19:3968-77 pubmed
    ..We propose that this phosphorylation targets srw1p for proteolysis and inhibits its activity to promote cdc13p turnover. ..
  35. Francesconi S, Grenon M, Bouvier D, Baldacci G. p56(chk1) protein kinase is required for the DNA replication checkpoint at 37 degrees C in fission yeast. EMBO J. 1997;16:1332-41 pubmed
    ..not to be necessary for the DNA replication checkpoint which acts through the inhibitory phosphorylation of p34(cdc2) kinase activity...
  36. Hentges P, Waller H, Reis C, Ferreira M, Doherty A. Cdk1 restrains NHEJ through phosphorylation of XRCC4-like factor Xlf1. Cell Rep. 2014;9:2011-7 pubmed publisher
    ..Together, these data establish that Xlf1 phosphorylation by Cdc2(Cdk1) provides a molecular mechanism for downregulation of NHEJ in fission yeast and indicates that XLF is a key ..
  37. Sakai T. Cdk inhibitor ste9p/srw1p is involved in response to protein synthesis inhibition in fission yeast. Biochem Biophys Res Commun. 2004;315:984-90 pubmed
    ..synthesis inhibitors, deltaste9 and some other mutants delay halting the cell cycle at G2-M and the combined cdc2-M26 deltaste9 mutant greatly loses viability...
  38. Ottilie S, Chernoff J, Hannig G, Hoffman C, Erikson R. The fission yeast genes pyp1+ and pyp2+ encode protein tyrosine phosphatases that negatively regulate mitosis. Mol Cell Biol. 1992;12:5571-80 pubmed
    ..pyp2+ in wild-type strains leads to a delay in mitosis but is suppressed by a wee1-50 mutation at 35 degrees C or a cdc2-1w mutation. A pyp1 disruption suppresses the temperature-sensitive lethality of a cdc25-22 mutation...
  39. Alemany V, Sanchez Piris M, Bachs O, Aligue R. Cmk2, a novel serine/threonine kinase in fission yeast. FEBS Lett. 2002;524:79-86 pubmed
    ..essential for cell viability but overexpression of cmk2(+) blocks the cell cycle at G2 phase and this inhibition is cdc2-dependent...
  40. Rowley R, Hudson J, Young P. The wee1 protein kinase is required for radiation-induced mitotic delay. Nature. 1992;356:353-5 pubmed
    ..Similarly, in fission yeast, the cdc25 and cdc2 gene products influence the ability of cells to delay mitosis in response to the inhibition of DNA synthesis...
  41. Forbes K, Humphrey T, Enoch T. Suppressors of cdc25p overexpression identify two pathways that influence the G2/M checkpoint in fission yeast. Genetics. 1998;150:1361-75 pubmed
    ..These results suggest that chk1(+) and rad24(+) may function redundantly with cds1(+) in the checkpoint response to unreplicated DNA. ..
  42. Ahn J, Won M, Choi J, Kyun M, Cho H, Park H, et al. Small heat-shock protein Hsp9 has dual functions in stress adaptation and stress-induced G2-M checkpoint regulation via Cdc25 inactivation in Schizosaccharomyces pombe. Biochem Biophys Res Commun. 2012;417:613-8 pubmed publisher
    ..of hsp9 induced cell cycle delay, increased the population of G2 phase cells, and rescued the phenotypes of cdc2-33, cdc25-22, ?rad24, and ?rad25 mutants, suggesting that Hsp9 probably regulates Cdc2 phosphorylation by ..
  43. Sveiczer A, Novak B, Mitchison J. The size control of fission yeast revisited. J Cell Sci. 1996;109 ( Pt 12):2947-57 pubmed
    ..First, induction synchronisation by block and release of cdc2 and cdc10. In the largest oversize-cells which are produced, the RCP is pushed back to the beginning of the cycle...
  44. Gilbreth M, Yang P, Bartholomeusz G, Pimental R, Kansra S, Gadiraju R, et al. Negative regulation of mitosis in fission yeast by the shk1 interacting protein skb1 and its human homolog, Skb1Hs. Proc Natl Acad Sci U S A. 1998;95:14781-6 pubmed
    ..pombe. Our results indicate that Skb1 negatively regulates mitosis by a mechanism that is independent of the Cdc2-activating phosphatase Cdc25 but that is at least partially dependent on Shk1 and the Cdc2 inhibitory kinase Wee1...
  45. Lahoz A, Alcaide Gavilán M, Daga R, Jimenez J. Antagonistic roles of PP2A-Pab1 and Etd1 in the control of cytokinesis in fission yeast. Genetics. 2010;186:1261-70 pubmed publisher
    ..Pab1 is also required to activate polarized cell growth. Thus, PP2A-Pab1 may be involved in coordinating polar growth and cytokinesis. ..
  46. Woollard A, Basi G, Nurse P. A novel S phase inhibitor in fission yeast. EMBO J. 1996;15:4603-12 pubmed
    ..We conclude that p14spd1 is a negative regulator of S phase, and that it may be part of the control ensuring an orderly onset of S phase or part of a G1-->S phase checkpoint control. ..
  47. Moris N, Shrivastava J, Jeffery L, Li J, Hayles J, Nurse P. A genome-wide screen to identify genes controlling the rate of entry into mitosis in fission yeast. Cell Cycle. 2016;15:3121-3130 pubmed
    ..These included mutants of the cdc2, cdc25, wee1 and pom1 genes, which have previously been shown to play a role in the timing of entry into mitosis, ..
  48. Moiseeva V, Amelina H, Collopy L, Armstrong C, Pearson S, Tomita K. The telomere bouquet facilitates meiotic prophase progression and exit in fission yeast. Cell Discov. 2017;3:17041 pubmed publisher
    ..Rather, loss of chromosome contact with the SPB caused delayed accumulation of CDK1-cyclin B at the SPB, which correlated with impaired SPB separation...
  49. Parker L, Atherton Fessler S, Piwnica Worms H. p107wee1 is a dual-specificity kinase that phosphorylates p34cdc2 on tyrosine 15. Proc Natl Acad Sci U S A. 1992;89:2917-21 pubmed
    ..These results indicate that p107wee1 functions as a mitotic inhibitor by directly phosphorylating p34cdc2 on Tyr-15 and that the preferred substrate for phosphorylation is the p34cdc2/cyclin complex. ..
  50. Kumar S, Huberman J. On the slowing of S phase in response to DNA damage in fission yeast. J Biol Chem. 2004;279:43574-80 pubmed
    ..Here we present evidence that a major downstream target of this pathway is the cyclin-dependent kinase, Cdc2. We also present evidence suggesting that the intra-S-phase checkpoint makes a relatively minor contribution to the ..
  51. Bueno A, Richardson H, Reed S, Russell P. A fission yeast B-type cyclin functioning early in the cell cycle. Cell. 1991;66:149-59 pubmed
    ..cerevisiae strain lacking CLN Start cyclins. Thus, cig1+ identifies a new class of B-type cyclin acting in G1 or S phase that appears to be functionally distinct from all previously described cyclin proteins. ..
  52. Parker L, Atherton Fessler S, Lee M, Ogg S, Falk J, Swenson K, et al. Cyclin promotes the tyrosine phosphorylation of p34cdc2 in a wee1+ dependent manner. EMBO J. 1991;10:1255-63 pubmed
    ..These results suggest that cyclin functions to facilitate tyrosine phosphorylation of p34cdc2 and that p107wee1 functions to regulate p34cdc2, either directly or indirectly, by tyrosine phosphorylation. ..
  53. Yamano H, Ishii K, Yanagida M. Phosphorylation of dis2 protein phosphatase at the C-terminal cdc2 consensus and its potential role in cell cycle regulation. EMBO J. 1994;13:5310-8 pubmed
    ..was abolished in the alanine substitution mutant at the C-terminal T316, which is conserved as a residue in the cdc2 consensus, TPPR, in a number of type 1-like phosphatases...
  54. Maiorano D, Van Assendelft G, Kearsey S. Fission yeast cdc21, a member of the MCM protein family, is required for onset of S phase and is located in the nucleus throughout the cell cycle. EMBO J. 1996;15:861-72 pubmed
    ..These results are consistent with the view that individual proteins in the MCM family are required for all initiation events, and defective initiation may impair the coordination between mitosis and S phase. ..
  55. Swaffer M, Jones A, Flynn H, Snijders A, Nurse P. CDK Substrate Phosphorylation and Ordering the Cell Cycle. Cell. 2016;167:1750-1761.e16 pubmed publisher
    ..Comparative analysis with wild-type cells expressing multiple cyclin-CDK complexes reveals how cyclin-substrate specificity works alongside activity thresholds to fine-tune the patterns of substrate phosphorylation. ..
  56. Alfa C, Booher R, Beach D, Hyams J. Fission yeast cyclin: subcellular localisation and cell cycle regulation. J Cell Sci Suppl. 1989;12:9-19 pubmed
    ..Schizosaccharomyces pombe involves the interaction of a number of genes with the major cell cycle control gene, cdc2+. One of these, cdc13+, encodes a protein with homology to cyclin...
  57. Okada N, Toda T, Yamamoto M, Sato M. CDK-dependent phosphorylation of Alp7-Alp14 (TACC-TOG) promotes its nuclear accumulation and spindle microtubule assembly. Mol Biol Cell. 2014;25:1969-82 pubmed publisher
    ..Thus our results reveal one way that CDK regulates spindle assembly at mitotic entry: CDK phosphorylates the Alp7-Alp14 complex to localize it to the nucleus. ..
  58. Shimada M, Namikawa Yamada C, Nakanishi M, Murakami H. Regulation of Cdc2p and Cdc13p is required for cell cycle arrest induced by defective RNA splicing in fission yeast. J Biol Chem. 2005;280:32640-8 pubmed
    ..The abundance of Cdc13p was reduced, and the phosphorylation of Cdc2p on tyrosine 15 was maintained in splicing-defective cells. These results suggest that regulation of Cdc13p and Cdc2p is required for G2 arrest in splicing mutants. ..
  59. Yamaguchi S, Murakami H, Okayama H. A WD repeat protein controls the cell cycle and differentiation by negatively regulating Cdc2/B-type cyclin complexes. Mol Biol Cell. 1997;8:2475-86 pubmed
    In the fission yeast Schizosaccharomyces pombe, p34(cdc2) plays a central role controlling the cell cycle...
  60. Synnes M, Nilssen E, Boye E, Grallert B. A novel chk1-dependent G1/M checkpoint in fission yeast. J Cell Sci. 2002;115:3609-18 pubmed
    ..We show that in G1-arrested orp1-4 cells, Wee1 phosphorylates and inactivates Cdc2. Activation of Chk1 occurs earlier than Cdc2 phosphorylation, indicating a novel role for Chk1, namely to induce ..
  61. Nakamura T, Moser B, Du L, Russell P. Cooperative control of Crb2 by ATM family and Cdc2 kinases is essential for the DNA damage checkpoint in fission yeast. Mol Cell Biol. 2005;25:10721-30 pubmed
    ..Importantly, we show that inactivation of Cdc2 in gamma-H2A-defective cells impairs Crb2-dependent signaling to the checkpoint kinase Chk1...
  62. Broadus M, Gould K. Multiple protein kinases influence the redistribution of fission yeast Clp1/Cdc14 phosphatase upon genotoxic stress. Mol Biol Cell. 2012;23:4118-28 pubmed publisher
    The Cdc14 phosphatase family antagonizes Cdk1 phosphorylation and is important for mitotic exit. To access their substrates, Cdc14 phosphatases are released from nucleolar sequestration during mitosis...
  63. Munoz M, Jimenez J. Genetic interactions between Hsp90 and the Cdc2 mitotic machinery in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet. 1999;261:242-50 pubmed
    In Schizosaccharomyces pombe, wee1 encodes a tyrosine kinase that inhibits entry into mitosis by phophorylating Cdc2, the universal cyclin-dependent kinase (Cdk) that regulates the G2/M transition in all eukaryotic cells...
  64. Mahyous Saeyd S, Ewert Krzemieniewska K, Liu B, Caspari T. Hyperactive Cdc2 kinase interferes with the response to broken replication forks by trapping S.pombe Crb2 in its mitotic T215 phosphorylated state. Nucleic Acids Res. 2014;42:7734-47 pubmed publisher
    Although it is well established that Cdc2 kinase phosphorylates the DNA damage checkpoint protein Crb2(53BP1) in mitosis, the full impact of this modification is still unclear...
  65. Vas A, Mok W, Leatherwood J. Control of DNA rereplication via Cdc2 phosphorylation sites in the origin recognition complex. Mol Cell Biol. 2001;21:5767-77 pubmed
    b>Cdc2 kinase is a master regulator of cell cycle progression in the fission yeast Schizosaccharomyces pombe. Our data indicate that Cdc2 phosphorylates replication factor Orp2, a subunit of the origin recognition complex (ORC)...
  66. Brown G, Jallepalli P, Huneycutt B, Kelly T. Interaction of the S phase regulator cdc18 with cyclin-dependent kinase in fission yeast. Proc Natl Acad Sci U S A. 1997;94:6142-7 pubmed
    ..The activity was identified by a variety of methods as the cyclin-dependent kinase containing the product of the cdc2(+) gene...
  67. Grallert A, Grallert B, Ribar B, Sipiczki M. Coordination of initiation of nuclear division and initiation of cell division in Schizosaccharomyces pombe: genetic interactions of mutations. J Bacteriol. 1998;180:892-900 pubmed
    ..Here we report on genetic interactions of sep1-1 with the M-phase initiation mutations wee1-, cdc2-1w, and cdc25-22...
  68. Iino Y, Hiramine Y, Yamamoto M. The role of cdc2 and other genes in meiosis in Schizosaccharomyces pombe. Genetics. 1995;140:1235-45 pubmed
    The requirement of the cdc2, cdc13 and cdc25 genes for meiosis in Schizosaccharomyces pombe was investigated using three different conditions to induce meiosis. These genes were known to be required for meiosis II...
  69. Munoz M, Bejarano E, Daga R, Jimenez J. The identification of Wos2, a p23 homologue that interacts with Wee1 and Cdc2 in the mitotic control of fission yeasts. Genetics. 1999;153:1561-72 pubmed
    The Wee1 kinase inhibits entry into mitosis by phosphorylation of the Cdc2 kinase...
  70. Booher R, Beach D. Site-specific mutagenesis of cdc2+, a cell cycle control gene of the fission yeast Schizosaccharomyces pombe. Mol Cell Biol. 1986;6:3523-30 pubmed
    The cdc2+ gene of Schizosaccharomyces pombe is homologous to the CDC28 gene of Saccharomyces cerevisiae. Both genes share limited homology with vertebrate protein kinases and have protein kinase activity...
  71. Baber Furnari B, Rhind N, Boddy M, Shanahan P, Lopez Girona A, Russell P. Regulation of mitotic inhibitor Mik1 helps to enforce the DNA damage checkpoint. Mol Biol Cell. 2000;11:1-11 pubmed
    ..Chk1 regulates the activity and localization of Cdc25, the tyrosine phosphatase that activates the cdk Cdc2. Here we report that Mik1, a tyrosine kinase that inhibits Cdc2, is positively regulated by the DNA damage ..
  72. Devos M, Mommaerts E, Migeot V, van Bakel H, Hermand D. Fission yeast Cdk7 controls gene expression through both its CAK and C-terminal domain kinase activities. Mol Cell Biol. 2015;35:1480-90 pubmed publisher
    ..The two enzymes have been proposed to act redundantly to activate Cdc2. Using an improved analogue-sensitive Mcs6-as kinase, we show that Csk1 is not a relevant CAK for Cdc2...
  73. Blanco M, Sanchez Diaz A, de Prada J, Moreno S. APC(ste9/srw1) promotes degradation of mitotic cyclins in G(1) and is inhibited by cdc2 phosphorylation. EMBO J. 2000;19:3945-55 pubmed
    ..We also show that ste9/srw1 is negatively regulated by cdc2-dependent protein phosphorylation...