Gene Symbol: alp14
Description: microtubule polymerase alp14
Alias: mtc1, microtubule polymerase alp14
Species: fission yeast

Top Publications

  1. Radcliffe P, Hirata D, Childs D, Vardy L, Toda T. Identification of novel temperature-sensitive lethal alleles in essential beta-tubulin and nonessential alpha 2-tubulin genes as fission yeast polarity mutants. Mol Biol Cell. 1998;9:1757-71 pubmed
    ..Therefore, it appears that the ts nda3 mutant becomes temperature lethal because of irreversible progression through the cell cycle in the absence of activating the spindle assembly checkpoint pathway. ..
  2. Nakaseko Y, Goshima G, Morishita J, Yanagida M. M phase-specific kinetochore proteins in fission yeast: microtubule-associating Dis1 and Mtc1 display rapid separation and segregation during anaphase. Curr Biol. 2001;11:537-49 pubmed
    ..We here show that fission yeast Dis1 and the related protein Mtc1/Alp14 are both able to bind microtubules in vitro and share an essential function for viability in vivo...
  3. Kakui Y, Sato M, Okada N, Toda T, Yamamoto M. Microtubules and Alp7-Alp14 (TACC-TOG) reposition chromosomes before meiotic segregation. Nat Cell Biol. 2013;15:786-96 pubmed publisher
    ..Moreover, the microtubule-associated protein complex Alp7-Alp14 (the fission yeast orthologues of mammalian TACC-TOG) is phosphorylated by Polo kinase, which promotes its meiosis-..
  4. Gard D, Becker B, Josh Romney S. MAPping the eukaryotic tree of life: structure, function, and evolution of the MAP215/Dis1 family of microtubule-associated proteins. Int Rev Cytol. 2004;239:179-272 pubmed
    ..cerevisiae, Dis1 and Alp14 in S. pombe), Dictyostelium (DdCP224), plants (Mor1 in A. thaliana and TMBP200 in N...
  5. Ling Y, Vjestica A, Oliferenko S. Nucleocytoplasmic shuttling of the TACC protein Mia1p/Alp7p is required for remodeling of microtubule arrays during the cell cycle. PLoS ONE. 2009;4:e6255 pubmed publisher
    ..We propose that intricate regulation of the subcellular distribution of TACC/TOG complexes drives microtubule array remodeling as cells progress through the cell cycle. ..
  6. Okada N, Toda T, Yamamoto M, Sato M. CDK-dependent phosphorylation of Alp7-Alp14 (TACC-TOG) promotes its nuclear accumulation and spindle microtubule assembly. Mol Biol Cell. 2014;25:1969-82 pubmed publisher
    ..In the closed mitosis of fission yeast, a microtubule-associated protein complex, Alp7-Alp14 (transforming acidic coiled-coil-tumor overexpressed gene), enters the nucleus upon mitotic entry and promotes ..
  7. Sato M, Toda T. Alp7/TACC is a crucial target in Ran-GTPase-dependent spindle formation in fission yeast. Nature. 2007;447:334-7 pubmed
    ..Here we show that a microtubule-associated protein, Alp7, which forms a complex with Alp14, is a target of Ran in yeast for spindle formation...
  8. Aoki K, Nakaseko Y, Kinoshita K, Goshima G, Yanagida M. CDC2 phosphorylation of the fission yeast dis1 ensures accurate chromosome segregation. Curr Biol. 2006;16:1627-35 pubmed
    ..form of Dis1(WT), whereas Dis1(6A) can partially rescue the phenotype resulting form deletion of Mtc1/Alp14, another XMAP215-like protein...
  9. Kerres A, Vietmeier Decker C, Ortiz J, Karig I, Beuter C, Hegemann J, et al. The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association. Mol Biol Cell. 2004;15:5255-67 pubmed
    ..Spc7 function seems to be conserved because, Spc105, a Saccharomyces cerevisiae homolog of Spc7, identified by mass spectrometry as a component of the conserved Ndc80 complex, can rescue mal3 mutant strains. ..

More Information


  1. Garcia M, Vardy L, Koonrugsa N, Toda T. Fission yeast ch-TOG/XMAP215 homologue Alp14 connects mitotic spindles with the kinetochore and is a component of the Mad2-dependent spindle checkpoint. EMBO J. 2001;20:3389-401 pubmed
    The TOG/XMAP215-related proteins play a role in microtubule dynamics at its plus end. Fission yeast Alp14, a newly identified TOG/XMAP215 family protein, is essential for proper chromosome segregation in concert with a second homologue ..
  2. Sato M, Vardy L, Angel Garcia M, Koonrugsa N, Toda T. Interdependency of fission yeast Alp14/TOG and coiled coil protein Alp7 in microtubule localization and bipolar spindle formation. Mol Biol Cell. 2004;15:1609-22 pubmed
    The Dis1/TOG family plays a pivotal role in microtubule organization. In fission yeast, Alp14 and Dis1 share an essential function in bipolar spindle formation...
  3. Sato M, Okada N, Kakui Y, Yamamoto M, Yoshida M, Toda T. Nucleocytoplasmic transport of Alp7/TACC organizes spatiotemporal microtubule formation in fission yeast. EMBO Rep. 2009;10:1161-7 pubmed publisher
    ..Here, we investigate the behaviour of Alp7 and its binding partner, Alp14/TOG, throughout the cell cycle...
  4. Al Bassam J, Kim H, Flor Parra I, Lal N, Velji H, Chang F. Fission yeast Alp14 is a dose-dependent plus end-tracking microtubule polymerase. Mol Biol Cell. 2012;23:2878-90 pubmed publisher
    ..Here we show that Alp14, a XMAP215 orthologue in fission yeast, Schizosaccharomyces pombe, has properties of a MT polymerase...
  5. Tang N, Takada H, Hsu K, Toda T. The internal loop of fission yeast Ndc80 binds Alp7/TACC-Alp14/TOG and ensures proper chromosome attachment. Mol Biol Cell. 2013;24:1122-33 pubmed publisher
    ..impaired localization of the microtubule-associated protein complex Alp7/transforming acidic coiled coil (TACC)-Alp14/tumor-overexpressed gene (TOG) to the mitotic kinetochore...
  6. Garcia M, Koonrugsa N, Toda T. Spindle-kinetochore attachment requires the combined action of Kin I-like Klp5/6 and Alp14/Dis1-MAPs in fission yeast. EMBO J. 2002;21:6015-24 pubmed
    ..The TOG/XMAP215 family, Alp14 and Dis1 are known to stabilize microtubules and be required for the bivalent attachment of the kinetochore to the ..
  7. Tang N, Toda T. Alp7/TACC recruits kinesin-8-PP1 to the Ndc80 kinetochore protein for timely mitotic progression and chromosome movement. J Cell Sci. 2015;128:354-63 pubmed publisher
    ..We propose that the recruitment of kinesin-8-PP1 to the kinetochore through Alp7/TACC interaction plays a crucial role in regulation of timely mitotic progression and chromosome movement during anaphase A. ..
  8. Laporte D, Courtout F, Pinson B, Dompierre J, Salin B, Brocard L, et al. A stable microtubule array drives fission yeast polarity reestablishment upon quiescence exit. J Cell Biol. 2015;210:99-113 pubmed publisher
    ..Our work demonstrates that quiescent S. pombe cells assemble specific cytoskeleton structures that improve the swiftness of the transition back to proliferation. ..
  9. Hussmann F, Drummond D, Peet D, Martin D, Cross R. Alp7/TACC-Alp14/TOG generates long-lived, fast-growing MTs by an unconventional mechanism. Sci Rep. 2016;6:20653 pubmed publisher
    b>Alp14 is a TOG-family microtubule polymerase from S. pombe that tracks plus ends and accelerates their growth. To interrogate its mechanism, we reconstituted dynamically unstable single isoform S...
  10. Kerres A, Jakopec V, Fleig U. The conserved Spc7 protein is required for spindle integrity and links kinetochore complexes in fission yeast. Mol Biol Cell. 2007;18:2441-54 pubmed
    ..spc7 mutant phenotypes were partially rescued by extra alpha-tubulin or extra Mal2. Thus, Spc7 interacts genetically with the Mal2-containing Sim4 complex. ..
  11. Hou H, Zhou Z, Wang Y, Wang J, Kallgren S, Kurchuk T, et al. Csi1 links centromeres to the nuclear envelope for centromere clustering. J Cell Biol. 2012;199:735-44 pubmed publisher
    ..These studies thus define a molecular link between the centromere and nuclear envelope that is responsible for centromere clustering...
  12. Xu X, Nakazawa N, Yanagida M. Condensin HEAT subunits required for DNA repair, kinetochore/centromere function and ploidy maintenance in fission yeast. PLoS ONE. 2015;10:e0119347 pubmed publisher
    ..Five of these mutations reside in helix B of HEAT repeats. Two non-SMC condensin subunits, Cnd1 and Cnd3, are thus implicated in ploidy maintenance. ..