Gene Symbol: alp1
Description: tubulin-specific chaperone cofactor D Alp1
Alias: tubulin-specific chaperone cofactor D Alp1
Species: fission yeast
Hirata D, Masuda H, Eddison M, Toda T. Essential role of tubulin-folding cofactor D in microtubule assembly and its association with microtubules in fission yeast. EMBO J. 1998;17:658-66 pubmed
..Here we show that alp1+ encodes a homologue of cofactor D and executes a function essential for cell viability...
Radcliffe P, Hirata D, Childs D, Vardy L, Toda T. Identification of novel temperature-sensitive lethal alleles in essential beta-tubulin and nonessential alpha 2-tubulin genes as fission yeast polarity mutants. Mol Biol Cell. 1998;9:1757-71 pubmed
We have screened for temperature-sensitive (ts) fission yeast mutants with altered polarity (alp1-15)...
Radcliffe P, Hirata D, Vardy L, Toda T. Functional dissection and hierarchy of tubulin-folding cofactor homologues in fission yeast. Mol Biol Cell. 1999;10:2987-3001 pubmed
..analysis shows that Alp11(B) interacts with both alpha-tubulin and Alp21(E), but not with the cofactor D homologue Alp1, whereas Alp21(E) also interacts with Alp1(D)...
Radcliffe P, Vardy L, Toda T. A conserved small GTP-binding protein Alp41 is essential for the cofactor-dependent biogenesis of microtubules in fission yeast. FEBS Lett. 2000;468:84-8 pubmed
..Genetic analysis indicates that Alp41 plays a crucial role in the cofactor-dependent pathway, in which it functions upstream of the cofactor D homologue Alp1(D) and possibly in concert with Alp21(E).
Fedyanina O, Mardanov P, Tokareva E, McIntosh J, Grishchuk E. Chromosome segregation in fission yeast with mutations in the tubulin folding cofactor D. Curr Genet. 2006;50:281-94 pubmed
..This and other genetic interactions between cofactor D mutants and specific kinetochore and spindle components suggest their critical role in establishing the normal kinetochore-microtubule interface. ..
Ohi M, Feoktistova A, Ren L, Yip C, Cheng Y, Chen J, et al
. Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1. Mol Cell. 2007;28:871-85 pubmed
..Comparison of the APC/C with another multiprotein E3 ligase, the SCF complex, uncovers remarkable structural similarities. ..
Fedyanina O, Book A, Grishchuk E. Tubulin heterodimers remain functional for one cell cycle after the inactivation of tubulin-folding cofactor D in fission yeast cells. Yeast. 2009;26:235-47 pubmed publisher
..during G(1) or S phases of the cell cycle, and when it fails to function due to the temperature-sensitive mutation alp1-t1, cells are unable to segregate their chromosomes in the subsequent mitosis...
Mori R, Toda T. The dual role of fission yeast Tbc1/cofactor C orchestrates microtubule homeostasis in tubulin folding and acts as a GAP for GTPase Alp41/Arl2. Mol Biol Cell. 2013;24:1713-24, S1-8 pubmed publisher
..In addition, we found that Alp41 interacts with Alp1(D), the orthologue of cofactor D, specifically when in the GDP-bound form...