Gene Symbol: adf1
Description: cofilin
Alias: cof1, cofilin
Species: fission yeast

Top Publications

  1. Andrianantoandro E, Pollard T. Mechanism of actin filament turnover by severing and nucleation at different concentrations of ADF/cofilin. Mol Cell. 2006;24:13-23 pubmed
    ..also showed that cofilins from fission yeast, Acanthamoeba, and human sever actin filaments optimally at low-cofilin binding densities well below their K(d)s, but not at high binding densities...
  2. Bathe M, Chang F. Cytokinesis and the contractile ring in fission yeast: towards a systems-level understanding. Trends Microbiol. 2010;18:38-45 pubmed publisher
    ..A picture of a highly dynamic and integrated system consisting of overlapping networks is beginning to emerge, the detailed nature of which remains to be elucidated. ..
  3. Nakano K, Mabuchi I. Actin-depolymerizing protein Adf1 is required for formation and maintenance of the contractile ring during cytokinesis in fission yeast. Mol Biol Cell. 2006;17:1933-45 pubmed
    The role of the actin-depolymerizing factor (ADF)/cofilin-family protein Adf1 in cytokinesis of fission yeast cells was studied...
  4. Chan C, Beltzner C, Pollard T. Cofilin dissociates Arp2/3 complex and branches from actin filaments. Curr Biol. 2009;19:537-45 pubmed publisher
    ..This study investigates how cofilin and Arp2/3 complex, two main players in the dendritic nucleation model, interact to produce sharp spatial ..
  5. Nakano K, Satoh K, Morimatsu A, Ohnuma M, Mabuchi I. Interactions among a fimbrin, a capping protein, and an actin-depolymerizing factor in organization of the fission yeast actin cytoskeleton. Mol Biol Cell. 2001;12:3515-26 pubmed
    ..The overexpression of Fim1 also excluded the actin-depolymerizing factor Adf1 from the F-actin patches and inhibited the turnover of actin in these structures...
  6. Nakano K, Mabuchi I. Actin-capping protein is involved in controlling organization of actin cytoskeleton together with ADF/cofilin, profilin and F-actin crosslinking proteins in fission yeast. Genes Cells. 2006;11:893-905 pubmed
    ..Moreover, genetic analysis showed that CP controls actin dynamics together with ADF/cofilin and profilin...
  7. Skau C, Courson D, Bestul A, Winkelman J, Rock R, Sirotkin V, et al. Actin filament bundling by fimbrin is important for endocytosis, cytokinesis, and polarization in fission yeast. J Biol Chem. 2011;286:26964-77 pubmed publisher
    ..discovered that a principal role of Fim1 is to control localization of tropomyosin Cdc8, thereby facilitating cofilin-mediated filament turnover...
  8. McCullough B, Grintsevich E, Chen C, Kang H, Hutchison A, Henn A, et al. Cofilin-linked changes in actin filament flexibility promote severing. Biophys J. 2011;101:151-9 pubmed publisher
    The actin regulatory protein, cofilin, increases the bending and twisting elasticity of actin filaments and severs them...
  9. Chen Q, Pollard T. Actin filament severing by cofilin is more important for assembly than constriction of the cytokinetic contractile ring. J Cell Biol. 2011;195:485-98 pubmed publisher
    We created two new mutants of fission yeast cofilin to investigate why cytokinesis in many organisms depends on this small actin-binding protein...

More Information


  1. Nakano K, Kuwayama H, Kawasaki M, Numata O, Takaine M. GMF is an evolutionarily developed Adf/cofilin-super family protein involved in the Arp2/3 complex-mediated organization of the actin cytoskeleton. Cytoskeleton (Hoboken). 2010;67:373-82 pubmed publisher
    Actin-depolymerizing factor (ADF)/cofilin is widely expressed in eukaryotes and plays a central role in reorganizing the actin cytoskeleton by disassembling actin filaments...
  2. Skau C, Kovar D. Fimbrin and tropomyosin competition regulates endocytosis and cytokinesis kinetics in fission yeast. Curr Biol. 2010;20:1415-22 pubmed publisher
    ..the access of other essential proteins to filaments, including myosin motors, Arp2/3 complex, formin, and cofilin. It is therefore critical to establish mechanisms for regulating the actin filament binding of tropomyosin...
  3. Ti S, Pollard T. Purification of actin from fission yeast Schizosaccharomyces pombe and characterization of functional differences from muscle actin. J Biol Chem. 2011;286:5784-92 pubmed publisher
    ..These properties close the gap between the biochemistry and predictions made by mathematical models of endocytosis in S. pombe cells. ..
  4. Yamamoto T, Kobayashi Ooka Y, Zhou G, Kawamukai M. Identification and characterization of Csh3 as an SH3 protein that interacts with fission yeast Cap1. FEMS Yeast Res. 2015;15: pubmed publisher
    ..These results demonstrate that Cap1 in Schizo. pombe is a multifunctional protein that functions through interaction with Cap1 itself and other proteins including adenylyl cyclase, actin and Csh3. ..
  5. Chen Q, Pollard T. Actin filament severing by cofilin dismantles actin patches and produces mother filaments for new patches. Curr Biol. 2013;23:1154-62 pubmed publisher
    ..We tested the proposal that cofilin severs actin filaments during endocytosis in fission yeast cells using a mutant cofilin defective in severing...
  6. Christensen J, Hocky G, Homa K, Morganthaler A, Hitchcock Degregori S, Voth G, et al. Competition between Tropomyosin, Fimbrin, and ADF/Cofilin drives their sorting to distinct actin filament networks. elife. 2017;6: pubmed publisher
    ..By focusing on the stabilizing protein tropomyosin Cdc8, bundling protein fimbrin Fim1, and severing protein coffin Adf1, we examined how their pairwise and collective interactions with actin filaments regulate their activity and ..