zntA

Summary

Gene Symbol: zntA
Description: zinc, cobalt and lead efflux system
Alias: ECK3453, JW3434, yhhO
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Lee J, Bae H, Jeong J, Lee J, Yang Y, Hwang I, et al. Functional expression of a bacterial heavy metal transporter in Arabidopsis enhances resistance to and decreases uptake of heavy metals. Plant Physiol. 2003;133:589-96 pubmed
    ..We tested whether an Escherichia coli gene, ZntA, which encodes a Pb(II)/Cd(II)/Zn(II) pump, could be useful for developing plants with reduced heavy metal content...
  2. Zimmer J, Doyle D. Phospholipid requirement and pH optimum for the in vitro enzymatic activity of the E. coli P-type ATPase ZntA. Biochim Biophys Acta. 2006;1758:645-52 pubmed
    Detergent solubilization and purification of the E. coli heavy metal P-type ATPase ZntA yields an enzyme with reduced hydrolytic activity in vitro...
  3. Okkeri J, Laakkonen L, Haltia T. The nucleotide-binding domain of the Zn2+-transporting P-type ATPase from Escherichia coli carries a glycine motif that may be involved in binding of ATP. Biochem J. 2004;377:95-105 pubmed
    ..The N domain of ZntA, a Zn2+-translocating P-type ATPase from Escherichia coli, is approx...
  4. Beard S, Hashim R, Membrillo Hernandez J, Hughes M, Poole R. Zinc(II) tolerance in Escherichia coli K-12: evidence that the zntA gene (o732) encodes a cation transport ATPase. Mol Microbiol. 1997;25:883-91 pubmed
    ..sequence analysis revealed that the KnR cassette was located within the putative promoter region of an ORF (o732 or yhhO) predicted to encode a protein of 732 amino acids, similar to cation transport P-type ATPases in the Cpx-type ..
  5. Grass G, Fan B, Rosen B, Franke S, Nies D, Rensing C. ZitB (YbgR), a member of the cation diffusion facilitator family, is an additional zinc transporter in Escherichia coli. J Bacteriol. 2001;183:4664-7 pubmed
    ..ZitB is specifically induced by zinc. ZitB expression on a plasmid rendered zntA-disrupted E...
  6. Dutta S, Liu J, Stemmler A, Mitra B. Conservative and nonconservative mutations of the transmembrane CPC motif in ZntA: effect on metal selectivity and activity. Biochemistry. 2007;46:3692-703 pubmed
    b>ZntA from Escherichia coli belongs to the P1B-ATPase transporter family and mediates resistance to toxic levels of selected divalent metal ions. P1B-type ATPases can be divided into subgroups based on substrate cation selectivity...
  7. Okkeri J, Haltia T. The metal-binding sites of the zinc-transporting P-type ATPase of Escherichia coli. Lys693 and Asp714 in the seventh and eighth transmembrane segments of ZntA contribute to the coupling of metal binding and ATPase activity. Biochim Biophys Acta. 2006;1757:1485-95 pubmed
    b>ZntA is a P-type ATPase which transports Zn(2+), Pb(2+) and Cd(2+) out of the cell. Two cysteine-containing motifs, CAAC near the N-terminus and CPC in transmembrane helix 6, are involved in binding of the translocated metal...
  8. Liu J, Stemmler A, Fatima J, Mitra B. Metal-binding characteristics of the amino-terminal domain of ZntA: binding of lead is different compared to cadmium and zinc. Biochemistry. 2005;44:5159-67 pubmed
    b>ZntA from Escherichia coli, a P1-type ATPase, specifically transports Pb(II), Zn(II), and Cd(II). Most P1-type ATPases have an N-terminal domain that contains one or more copies of the conserved metal-binding motif, GXXCXXC...
  9. Brocklehurst K, Hobman J, Lawley B, Blank L, Marshall S, Brown N, et al. ZntR is a Zn(II)-responsive MerR-like transcriptional regulator of zntA in Escherichia coli. Mol Microbiol. 1999;31:893-902 pubmed
    We have identified the promoter/operator region of the zntA gene of Escherichia coli and shown that Zn(II) is the primary inducer of expression of this Zn(II)/Cd(II) export gene...

More Information

Publications29

  1. Okkeri J, Haltia T. Expression and mutagenesis of ZntA, a zinc-transporting P-type ATPase from Escherichia coli. Biochemistry. 1999;38:14109-16 pubmed
    ..We have constructed an expression system for one of them, ZntA, which encodes a 732 amino acid residue protein capable of transporting Zn(2+)...
  2. Sharma R, Rensing C, Rosen B, Mitra B. The ATP hydrolytic activity of purified ZntA, a Pb(II)/Cd(II)/Zn(II)-translocating ATPase from Escherichia coli. J Biol Chem. 2000;275:3873-8 pubmed
    b>ZntA, a soft metal-translocating P1-type ATPase from Escherichia coli, confers resistance to Pb(II), Cd(II), and Zn(II)...
  3. Hou Z, Mitra B. The metal specificity and selectivity of ZntA from Escherichia coli using the acylphosphate intermediate. J Biol Chem. 2003;278:28455-61 pubmed
    b>ZntA from Escherichia coli is a P-type ATPase that confers resistance to Pb(II), Zn(II), and Cd(II) in vivo. We had previously shown that purified ZntA shows ATP hydrolysis activity with the metal ions Pb(II), Zn(II), and Cd(II)...
  4. Okkeri J, Bencomo E, Pietilä M, Haltia T. Introducing Wilson disease mutations into the zinc-transporting P-type ATPase of Escherichia coli. The mutation P634L in the 'hinge' motif (GDGXNDXP) perturbs the formation of the E2P state. Eur J Biochem. 2002;269:1579-86 pubmed
    b>ZntA, a bacterial zinc-transporting P-type ATPase, is homologous to two human ATPases mutated in Menkes and Wilson diseases...
  5. Rensing C, Mitra B, Rosen B. The zntA gene of Escherichia coli encodes a Zn(II)-translocating P-type ATPase. Proc Natl Acad Sci U S A. 1997;94:14326-31 pubmed
    ..This gene, termed zntA, was disrupted by insertion of a kanamycin gene through homologous recombination...
  6. Mitra B, Sharma R. The cysteine-rich amino-terminal domain of ZntA, a Pb(II)/Zn(II)/Cd(II)-translocating ATPase from Escherichia coli, is not essential for its function. Biochemistry. 2001;40:7694-9 pubmed
    ..b>ZntA from Escherichia coli, a Pb(II)-, Zn(II)-, and Cd(II)-transporting ATPase, has an approximately 120 residue amino-..
  7. Binet M, Poole R. Cd(II), Pb(II) and Zn(II) ions regulate expression of the metal-transporting P-type ATPase ZntA in Escherichia coli. FEBS Lett. 2000;473:67-70 pubmed
    b>ZntA is a cation-translocating ATPase which exports from Escherichia coli Cd(II) and Pb(II), as well as Zn(II)...
  8. Outten C, Outten F, O Halloran T. DNA distortion mechanism for transcriptional activation by ZntR, a Zn(II)-responsive MerR homologue in Escherichia coli. J Biol Chem. 1999;274:37517-24 pubmed
    ..coli ZntR protein, a homologue of MerR, has recently been shown to mediate Zn(II)-responsive regulation of zntA, a gene involved in Zn(II) detoxification...
  9. Rensing C, Sun Y, Mitra B, Rosen B. Pb(II)-translocating P-type ATPases. J Biol Chem. 1998;273:32614-7 pubmed
    ..The Escherichia coli homologue of CadA, termed ZntA, is a Zn(II)/Cd(II) pump...
  10. Helbig K, Bleuel C, Krauss G, Nies D. Glutathione and transition-metal homeostasis in Escherichia coli. J Bacteriol. 2008;190:5431-8 pubmed publisher
    ..In mutant cells without the P-type ATPase ZntA, the additional deletion of the GSH biosynthesis system led to a strong decrease in resistance to Cd(II) and Zn(II)...
  11. Banci L, Bertini I, Ciofi Baffoni S, Finney L, Outten C, O Halloran T. A new zinc-protein coordination site in intracellular metal trafficking: solution structure of the Apo and Zn(II) forms of ZntA(46-118). J Mol Biol. 2002;323:883-97 pubmed
    ..metalloproteins, is shown here to adopt a novel coordination environment in the Escherichia coli transport protein ZntA. The ZntA protein is a P-type ATPase that pumps zinc out of the cytoplasm and into the periplasm...
  12. Dutta S, Liu J, Mitra B. Kinetic analysis of metal binding to the amino-terminal domain of ZntA by monitoring metal-thiolate charge-transfer complexes. Biochemistry. 2005;44:14268-74 pubmed
    b>ZntA, a P(1B)-ATPase transporter from Escherichia coli, mediates resistance specifically to Pb(2+), Zn(2+), and Cd(2+) by active efflux. ZntA has a hydrophilic N-terminal domain that binds one metal ion...
  13. Yamamoto K, Ishihama A. Transcriptional response of Escherichia coli to external zinc. J Bacteriol. 2005;187:6333-40 pubmed
    ..Addition of external zinc induced the expression of zinc exporter ZntA and inhibited the expression of zinc importer ZnuC...
  14. Liu J, Dutta S, Stemmler A, Mitra B. Metal-binding affinity of the transmembrane site in ZntA: implications for metal selectivity. Biochemistry. 2006;45:763-72 pubmed
    b>ZntA, a P1B-type ATPase, confers resistance specifically to Pb2+, Zn2+, and Cd2 in Escherichia coli...
  15. Dutta S, Liu J, Hou Z, Mitra B. Conserved aspartic acid 714 in transmembrane segment 8 of the ZntA subgroup of P1B-type ATPases is a metal-binding residue. Biochemistry. 2006;45:5923-31 pubmed
    b>ZntA from Escherichia coli is a member of the P1B-type ATPase family that confers resistance specifically to Pb2+, Zn2+, and Cd2 salts by active efflux across the cytoplasmic membrane...
  16. Lee L, Barrett J, Poole R. Genome-wide transcriptional response of chemostat-cultured Escherichia coli to zinc. J Bacteriol. 2005;187:1124-34 pubmed
    ..were significantly up-regulated by Zn stress, including genes known to be involved in Zn tolerance, particularly zntA, zraP, and hydG...
  17. Binet M, Cruz Ramos H, Laver J, Hughes M, Poole R. Nitric oxide releases intracellular zinc from prokaryotic metallothionein in Escherichia coli. FEMS Microbiol Lett. 2002;213:121-6 pubmed
    ..Using the zinc-responsive expression of zntA (encoding a Zn-exporting P-type ATPase) fused to lacZ, i.e...
  18. Hou Z, Narindrasorasak S, Bhushan B, Sarkar B, Mitra B. Functional analysis of chimeric proteins of the Wilson Cu(I)-ATPase (ATP7B) and ZntA, a Pb(II)/Zn(II)/Cd(II)-ATPase from Escherichia coli. J Biol Chem. 2001;276:40858-63 pubmed
    ATP7B, the Wilson disease-associated Cu(I)-transporter, and ZntA from Escherichia coli are soft metal P1-type ATPases with mutually exclusive metal ion substrates...
  19. Noll M, Lutsenko S. Expression of ZntA, a zinc-transporting P1-type ATPase, is specifically regulated by zinc and cadmium. IUBMB Life. 2000;49:297-302 pubmed
    ..Here, we demonstrate that the expression of ZntA, a Zn,Cd-transporting P-type ATPase of Escherichia coli, is specifically regulated by the transported cations, ..
  20. Wang D, Hosteen O, Fierke C. ZntR-mediated transcription of zntA responds to nanomolar intracellular free zinc. J Inorg Biochem. 2012;111:173-81 pubmed publisher
    In E. coli, ZitB and ZntA are important metal exporters that enhance cell viability under high environmental zinc...