zipA

Summary

Gene Symbol: zipA
Description: FtsZ stabilizer
Alias: ECK2407, JW2404
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Hale C, de Boer P. Direct binding of FtsZ to ZipA, an essential component of the septal ring structure that mediates cell division in E. coli. Cell. 1997;88:175-85 pubmed
    ..Here we report that FtsZ binds directly to a novel integral inner membrane protein in E. coli that we call ZipA. We present genetic and morphological evidence indicating that this interaction is required for cell division, and ..
  2. Geissler B, Elraheb D, Margolin W. A gain-of-function mutation in ftsA bypasses the requirement for the essential cell division gene zipA in Escherichia coli. Proc Natl Acad Sci U S A. 2003;100:4197-202 pubmed
    b>ZipA and FtsA are recruited independently to the FtsZ cytokinetic ring (Z ring) and are essential for cell division of Escherichia coli...
  3. Geissler B, Margolin W. Evidence for functional overlap among multiple bacterial cell division proteins: compensating for the loss of FtsK. Mol Microbiol. 2005;58:596-612 pubmed
    ..ftsA R286W or multicopy ftsQAZ, which can largely bypass the requirement for the essential cell division gene zipA, allowed cells with a complete deletion of ftsK to survive and divide, although many of these ftsK null cells ..
  4. Jennings L, Foreman K, Rush T, Tsao D, Mosyak L, Kincaid S, et al. Combinatorial synthesis of substituted 3-(2-indolyl)piperidines and 2-phenyl indoles as inhibitors of ZipA-FtsZ interaction. Bioorg Med Chem. 2004;12:5115-31 pubmed
    The ZipA-FtsZ protein-protein interaction is a potential target for antibacterial therapy. The design and parallel synthesis of a combinatorial library of small molecules, which target the FtsZ binding area on ZipA are described...
  5. Hale C, de Boer P. ZipA is required for recruitment of FtsK, FtsQ, FtsL, and FtsN to the septal ring in Escherichia coli. J Bacteriol. 2002;184:2552-6 pubmed
    ..least nine essential gene products whose order of assembly resembles a mostly linear dependency pathway: FtsA and ZipA directly bind FtsZ polymers at the prospective division site, followed by the sequential addition of FtsK, FtsQ, ..
  6. Jennings L, Foreman K, Rush T, Tsao D, Mosyak L, Li Y, et al. Design and synthesis of indolo[2,3-a]quinolizin-7-one inhibitors of the ZipA-FtsZ interaction. Bioorg Med Chem Lett. 2004;14:1427-31 pubmed
    The binding of FtsZ to ZipA is a potential target for antibacterial therapy...
  7. Pichoff S, Shen B, Sullivan B, Lutkenhaus J. FtsA mutants impaired for self-interaction bypass ZipA suggesting a model in which FtsA's self-interaction competes with its ability to recruit downstream division proteins. Mol Microbiol. 2012;83:151-67 pubmed publisher
    Z-ring assembly requires polymers of the tubulin homologue FtsZ to be tethered to the membrane. Although either ZipA or FtsA is sufficient to do this, both of these are required for recruitment of downstream proteins to form a functional ..
  8. Hale C, Rhee A, de Boer P. ZipA-induced bundling of FtsZ polymers mediated by an interaction between C-terminal domains. J Bacteriol. 2000;182:5153-66 pubmed
    FtsZ and ZipA are essential components of the septal ring apparatus, which mediates cell division in Escherichia coli. FtsZ is a cytoplasmic tubulin-like GTPase that forms protofilament-like homopolymers in vitro...
  9. Johnson J, Lackner L, Hale C, de Boer P. ZipA is required for targeting of DMinC/DicB, but not DMinC/MinD, complexes to septal ring assemblies in Escherichia coli. J Bacteriol. 2004;186:2418-29 pubmed
    ..Here we show that the FtsZ-binding protein ZipA is required for both recruitment of the (D)MinC/DicB complex to FtsZ rings and the DicB-inducible division block ..

More Information

Publications48

  1. Kuchibhatla A, Bhattacharya A, Panda D. ZipA binds to FtsZ with high affinity and enhances the stability of FtsZ protofilaments. PLoS ONE. 2011;6:e28262 pubmed publisher
    A bacterial membrane protein ZipA that tethers FtsZ to the membrane is known to promote FtsZ assembly. In this study, the binding of ZipA to FtsZ was monitored using fluorescence spectroscopy...
  2. Kenny C, Ding W, Kelleher K, Benard S, Dushin E, Sutherland A, et al. Development of a fluorescence polarization assay to screen for inhibitors of the FtsZ/ZipA interaction. Anal Biochem. 2003;323:224-33 pubmed
    ..polarization competition assay has been developed to screen for inhibitors of the Escherichia coli FtsZ/ZipA protein-protein interaction...
  3. Weiss D. Bacterial cell division and the septal ring. Mol Microbiol. 2004;54:588-97 pubmed
    ..Finally, two murein hydrolases, AmiC and EnvC, have been shown to localize to the septal ring of Escherichia coli, where they play an important role in separation of daughter cells. ..
  4. Pichoff S, Lutkenhaus J. Unique and overlapping roles for ZipA and FtsA in septal ring assembly in Escherichia coli. EMBO J. 2002;21:685-93 pubmed
    b>ZipA and FtsA are essential division proteins in Escherichia coli that are recruited to the division site by interaction with FtsZ...
  5. Mosyak L, Zhang Y, Glasfeld E, Haney S, Stahl M, Seehra J, et al. The bacterial cell-division protein ZipA and its interaction with an FtsZ fragment revealed by X-ray crystallography. EMBO J. 2000;19:3179-91 pubmed
    In Escherichia coli, FtsZ, a homologue of eukaryotic tubulins, and ZipA, a membrane-anchored protein that binds to FtsZ, are two essential components of the septal ring structure that mediates cell division...
  6. RayChaudhuri D. ZipA is a MAP-Tau homolog and is essential for structural integrity of the cytokinetic FtsZ ring during bacterial cell division. EMBO J. 1999;18:2372-83 pubmed
    ..Here I present genetic and morphological evidence that a 2-fold higher dosage of the division gene zipA suppresses thermosensitivity of the ftsZ84 mutant by stabilizing the labile FtsZ84 ring structure in vivo...
  7. Sikdar R, Doerrler W. Inefficient Tat-dependent export of periplasmic amidases in an Escherichia coli strain with mutations in two DedA family genes. J Bacteriol. 2010;192:807-18 pubmed publisher
    ..Green fluorescent protein fusions to the cell division proteins FtsZ, ZipA, FtsI, FtsL, and FtsQ are correctly localized to new septation sites in BC202...
  8. Liu Z, Mukherjee A, Lutkenhaus J. Recruitment of ZipA to the division site by interaction with FtsZ. Mol Microbiol. 1999;31:1853-61 pubmed
    b>ZipA is an essential cell division protein in Escherichia coli that is recruited to the division site early in the division cycle...
  9. Donachie W. Co-ordinate regulation of the Escherichia coli cell cycle or The cloud of unknowing. Mol Microbiol. 2001;40:779-85 pubmed
    ..A discussion of some aspects of the regulation of chromosome replication, segregation and cell division in Escherichia coli. ..
  10. Erickson H. The FtsZ protofilament and attachment of ZipA--structural constraints on the FtsZ power stroke. Curr Opin Cell Biol. 2001;13:55-60 pubmed
    ..b>ZipA, a membrane-tethered division protein, binds to a highly conserved short peptide on the carboxyl terminus of FtsZ...
  11. López Montero I, López Navajas P, Mingorance J, Rivas G, Velez M, Vicente M, et al. Intrinsic disorder of the bacterial cell division protein ZipA: coil-to-brush conformational transition. FASEB J. 2013;27:3363-75 pubmed publisher
    The full-length ZipA protein from Escherichia coli, one of the essential elements of the cell division machinery, was studied in a surface model built as adsorbed monolayers...
  12. Ohashi T, Hale C, de Boer P, Erickson H. Structural evidence that the P/Q domain of ZipA is an unstructured, flexible tether between the membrane and the C-terminal FtsZ-binding domain. J Bacteriol. 2002;184:4313-5 pubmed
    The cell division protein ZipA has an N-terminal transmembrane domain and a C-terminal globular domain that binds FtsZ...
  13. Haeusser D, Hoashi M, Weaver A, Brown N, Pan J, Sawitzke J, et al. The Kil peptide of bacteriophage ? blocks Escherichia coli cytokinesis via ZipA-dependent inhibition of FtsZ assembly. PLoS Genet. 2014;10:e1004217 pubmed publisher
    ..Cells with the normally essential cell division gene zipA deleted (in a modified background) display normal FtsZ rings after kil expression, suggesting that ZipA is required ..
  14. Justice S, Garcia Lara J, Rothfield L. Cell division inhibitors SulA and MinC/MinD block septum formation at different steps in the assembly of the Escherichia coli division machinery. Mol Microbiol. 2000;37:410-23 pubmed
    ..immunofluorescence microscopy was used to determine the effect of SulA and MinCD on the formation of FtsZ, FtsA and ZipA rings at potential division sites...
  15. Bernard C, Sadasivam M, Shiomi D, Margolin W. An altered FtsA can compensate for the loss of essential cell division protein FtsN in Escherichia coli. Mol Microbiol. 2007;64:1289-305 pubmed
    ..Remarkably, these altered FtsA proteins also could partially suppress a deletion of ftsK or zipA, were less toxic than wild-type FtsA when in excess, and conferred resistance to excess MinC, indicating that they ..
  16. Goehring N, Gonzalez M, Beckwith J. Premature targeting of cell division proteins to midcell reveals hierarchies of protein interactions involved in divisome assembly. Mol Microbiol. 2006;61:33-45 pubmed
    ..Based on these observations we present a revised model for assembly of the E. coli division apparatus. ..
  17. Mileykovskaya E, Sun Q, Margolin W, Dowhan W. Localization and function of early cell division proteins in filamentous Escherichia coli cells lacking phosphatidylethanolamine. J Bacteriol. 1998;180:4252-7 pubmed
    ..Several proteins essential for early steps in cytokinesis, such as FtsZ, ZipA, and FtsA, were able to localize as bands to potential division sites in pss-93 filaments, indicating that the ..
  18. Chen J, Weiss D, Ghigo J, Beckwith J. Septal localization of FtsQ, an essential cell division protein in Escherichia coli. J Bacteriol. 1999;181:521-30 pubmed
    ..In addition, the septal localization of ZipA apparently did not require functional FtsQ...
  19. Wang S, Arends S, Weiss D, Newman E. A deficiency in S-adenosylmethionine synthetase interrupts assembly of the septal ring in Escherichia coli K-12. Mol Microbiol. 2005;58:791-9 pubmed
    ..Examination with GFP fusions to various septal proteins shows that FtsZ, ZipA and FtsA localize to the septal ring, but FtsQ, FtsW, FtsI or FtsN do not...
  20. Badaluddin N, Kitakawa M. Escherichia coli inner membrane protein YciB interacts with ZipA that is important for cell division. Genes Cells. 2015;20:956-65 pubmed publisher
    ..Furthermore, the septum localization of ZipA, an essential protein of cell division, is disturbed in a ΔyciB mutant...
  21. Aarsman M, Piette A, Fraipont C, Vinkenvleugel T, Nguyen Distèche M, Den Blaauwen T. Maturation of the Escherichia coli divisome occurs in two steps. Mol Microbiol. 2005;55:1631-45 pubmed
    Cell division proteins FtsZ (FtsA, ZipA, ZapA), FtsE/X, FtsK, FtsQ, FtsL/B, FtsW, PBP3, FtsN and AmiC localize at mid cell in Escherichia coli in an interdependent order as listed...
  22. L pez Montero I, L pez Navajas P, Mingorance J, V lez M, Vicente M, Monroy F. Membrane reconstitution of FtsZ-ZipA complex inside giant spherical vesicles made of E. coli lipids: large membrane dilation and analysis of membrane plasticity. Biochim Biophys Acta. 2013;1828:687-98 pubmed publisher
    ..Membrane anchoring is mediated by ZipA, an essential transmembrane protein able to specifically bind FtsZ...
  23. Vollmer W. The prokaryotic cytoskeleton: a putative target for inhibitors and antibiotics?. Appl Microbiol Biotechnol. 2006;73:37-47 pubmed
    ..For example, the polyphenol dichamanetin, the 2-alkoxycarbonylaminopyridine SRI-3072, and the benzophenanthridine alkaloid sanguinarine inhibit the GTPase activity of FtsZ and exhibit antimicrobial activity. ..
  24. Moy F, Glasfeld E, Mosyak L, Powers R. Solution structure of ZipA, a crucial component of Escherichia coli cell division. Biochemistry. 2000;39:9146-56 pubmed
    b>ZipA, an essential component of cell division in Escherichia coli, interacts with the FtsZ protein at the midcell in one of the initial steps of septum formation...
  25. Schmidt K, Peterson N, Kustusch R, Wissel M, Graham B, Phillips G, et al. A predicted ABC transporter, FtsEX, is needed for cell division in Escherichia coli. J Bacteriol. 2004;186:785-93 pubmed
    ..Localization of FtsX was studied in detail and appeared to require FtsZ, FtsA, and ZipA, but not the downstream division proteins FtsK, FtsQ, FtsL, and FtsI...
  26. Tsao D, Sutherland A, Jennings L, Li Y, Rush T, Alvarez J, et al. Discovery of novel inhibitors of the ZipA/FtsZ complex by NMR fragment screening coupled with structure-based design. Bioorg Med Chem. 2006;14:7953-61 pubmed
    b>ZipA is a membrane anchored protein in Escherichia coli that interacts with FtsZ, a homolog of eukaryotic tubulins, forming a septal ring structure that mediates bacterial cell division...
  27. Ghigo J, Weiss D, Chen J, Yarrow J, Beckwith J. Localization of FtsL to the Escherichia coli septal ring. Mol Microbiol. 1999;31:725-37 pubmed
    ..In a reverse approach, we use fusions of green fluorescent protein (GFP) to FtsZ, FtsA and ZipA to show that these proteins localize to the division site in an FtsL-independent fashion...
  28. Rush T, Grant J, Mosyak L, Nicholls A. A shape-based 3-D scaffold hopping method and its application to a bacterial protein-protein interaction. J Med Chem. 2005;48:1489-95 pubmed
    ..program ROCS (Rapid Overlay of Chemical Structures) to find new scaffolds for small molecule inhibitors of the ZipA-FtsZ protein-protein interaction, a proposed antibacterial target...
  29. Di Lallo G, Fagioli M, Barionovi D, Ghelardini P, Paolozzi L. Use of a two-hybrid assay to study the assembly of a complex multicomponent protein machinery: bacterial septosome differentiation. Microbiology. 2003;149:3353-9 pubmed
    The ability of each of the nine Escherichia coli division proteins (FtsZ, FtsA, ZipA, FtsK, FtsQ, FtsL, FtsW, FtsI, FtsN) to interact with itself and with each of the remaining eight proteins was studied in 43 possible combinations of ..
  30. Vega D, Margolin W. Suppression of a Thermosensitive zipA Cell Division Mutant by Altering Amino Acid Metabolism. J Bacteriol. 2018;200: pubmed publisher
    b>ZipA is essential for cell division in Escherichia coli, acting early in the process to anchor polymers of FtsZ to the cytoplasmic membrane...
  31. Rueda S, Vicente M, Mingorance J. Concentration and assembly of the division ring proteins FtsZ, FtsA, and ZipA during the Escherichia coli cell cycle. J Bacteriol. 2003;185:3344-51 pubmed
    The concentration of the cell division proteins FtsZ, FtsA, and ZipA and their assembly into a division ring during the Escherichia coli B/r K cell cycle have been measured in synchronous cultures obtained by the membrane elution ..
  32. Ebersbach G, Galli E, Møller Jensen J, Lowe J, Gerdes K. Novel coiled-coil cell division factor ZapB stimulates Z ring assembly and cell division. Mol Microbiol. 2008;68:720-35 pubmed publisher
    ..Localization of ZapB to the divisome depended on FtsZ but not FtsA, ZipA or FtsI, and ZapB interacted with FtsZ in a bacterial two-hybrid analysis...
  33. Vicente M, Rico A. The order of the ring: assembly of Escherichia coli cell division components. Mol Microbiol. 2006;61:5-8 pubmed
    ..In addition, back-recruitment of all late proteins except FtsN into the division ring occurs even in the absence of proteins incorporated at earlier stages, i.e. FtsA or FtsQ. ..
  34. Skoog K, Daley D. The Escherichia coli cell division protein ZipA forms homodimers prior to association with FtsZ. Biochemistry. 2012;51:1407-15 pubmed publisher
    b>ZipA is an essential component of the cell division machinery in E. coli and other closely related bacteria. It is an integral membrane protein that binds to FtsZ, tethering it to the inner membrane...
  35. Shiomi D, Niki H. A mutation in the promoter region of zipA, a component of the divisome, suppresses the shape defect of RodZ-deficient cells. Microbiologyopen. 2013;2:798-810 pubmed publisher
    ..One of the mutations was in the promoter region of zipA, which encodes a crucial component of the cell division machinery...
  36. Shen B, Lutkenhaus J. The conserved C-terminal tail of FtsZ is required for the septal localization and division inhibitory activity of MinC(C)/MinD. Mol Microbiol. 2009;72:410-24 pubmed
    ..This tail also mediates interaction with two essential division proteins, ZipA and FtsA, to link FtsZ polymers to the membrane...
  37. Errington J, Daniel R, Scheffers D. Cytokinesis in bacteria. Microbiol Mol Biol Rev. 2003;67:52-65, table of contents pubmed
    ..The cytoplasmic proteins are linked to the membrane by putative membrane anchor proteins, such as ZipA of E. coli and possibly EzrA of B. subtilis, which have a single membrane span but a cytoplasmic C-terminal domain...
  38. Ma X, Margolin W. Genetic and functional analyses of the conserved C-terminal core domain of Escherichia coli FtsZ. J Bacteriol. 1999;181:7531-44 pubmed
    In Escherichia coli, FtsZ is required for the recruitment of the essential cell division proteins FtsA and ZipA to the septal ring. Several C-terminal deletions of E...
  39. Hale C, de Boer P. Recruitment of ZipA to the septal ring of Escherichia coli is dependent on FtsZ and independent of FtsA. J Bacteriol. 1999;181:167-76 pubmed
    ..In Escherichia coli, this organelle consists of several essential division proteins, including FtsZ, FtsA, and ZipA. To gain more insight into how the structure is assembled, we studied the interdependence of FtsZ, FtsA, and ZipA ..