Gene Symbol: ygbT
Description: multifunctional endonuclease Cas1, CRISPR adaptation protein; DNA repair enzyme
Alias: ECK2750, JW2725, cas1
Species: Escherichia coli str. K-12 substr. MG1655
Products:     ygbT

Top Publications

  1. Brouns S, Jore M, Lundgren M, Westra E, Slijkhuis R, Snijders A, et al. Small CRISPR RNAs guide antiviral defense in prokaryotes. Science. 2008;321:960-4 pubmed publisher
    ..Our results demonstrate that the formation of mature guide RNAs by the CRISPR RNA endonuclease subunit of Cascade is a mechanistic requirement for antiviral defense...
  2. Wiedenheft B, Zhou K, Jinek M, Coyle S, Ma W, Doudna J. Structural basis for DNase activity of a conserved protein implicated in CRISPR-mediated genome defense. Structure. 2009;17:904-12 pubmed publisher
    ..CRISPR-associated gene 1 (cas1) encodes the only universally conserved protein component of CRISPR immune systems, yet its function is unknown...
  3. Babu M, Beloglazova N, Flick R, Graham C, Skarina T, Nocek B, et al. A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair. Mol Microbiol. 2011;79:484-502 pubmed publisher
    ..b>Cas1 is a CRISPR-associated protein that is common to all CRISPR-containing prokaryotes but its function remains obscure...
  4. YOUNG R. Molecular biology. Secret weapon. Science. 2008;321:922-3 pubmed publisher
  5. Marraffini L, Sontheimer E. Invasive DNA, chopped and in the CRISPR. Structure. 2009;17:786-8 pubmed publisher
    In this issue of Structure, Wiedenheft et al. describe the structure and activity of Cas1, the only protein associated with all CRISPR loci...
  6. Nu ez J, Kranzusch P, Noeske J, Wright A, Davies C, Doudna J. Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity. Nat Struct Mol Biol. 2014;21:528-34 pubmed publisher
    ..The nucleases Cas1 and Cas2 are the only proteins conserved among all CRISPR-Cas systems, yet the molecular functions of these ..
  7. Rollie C, Schneider S, Brinkmann A, Bolt E, White M. Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition. elife. 2015;4: pubmed publisher
    ..DNA for incorporation into the genome at the CRISPR locus, a process known as Adaptation, which is dependent on the Cas1 and Cas2 proteins...
  8. Musharova O, Klimuk E, Datsenko K, Metlitskaya A, Logacheva M, Semenova E, et al. Spacer-length DNA intermediates are associated with Cas1 in cells undergoing primed CRISPR adaptation. Nucleic Acids Res. 2017;45:3297-3307 pubmed publisher
    ..A complex of Cas1 and Cas2 proteins is capable of inserting new spacers in the CRISPR array...
  9. Wang J, Li J, Zhao H, Sheng G, Wang M, Yin M, et al. Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems. Cell. 2015;163:840-53 pubmed publisher
    ..We report on the structures of Cas1-Cas2-dual-forked DNA complexes in an effort toward understanding how the protospacer is sampled prior to insertion ..

More Information


  1. Redding S, Sternberg S, Marshall M, Gibb B, Bhat P, Guegler C, et al. Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System. Cell. 2015;163:854-65 pubmed publisher
    ..elicit an alternative pathway that recruits a nuclease-inactive Cas3 through a mechanism that is dependent on the Cas1 and Cas2 proteins...
  2. Wright A, Liu J, Knott G, Doxzen K, Nogales E, Doudna J. Structures of the CRISPR genome integration complex. Science. 2017;357:1113-1118 pubmed publisher
    CRISPR-Cas systems depend on the Cas1-Cas2 integrase to capture and integrate short foreign DNA fragments into the CRISPR locus, enabling adaptation to new viruses...
  3. Westra E, van Houte S, Oyesiku Blakemore S, Makin B, Broniewski J, Best A, et al. Parasite Exposure Drives Selective Evolution of Constitutive versus Inducible Defense. Curr Biol. 2015;25:1043-9 pubmed publisher
    ..Using a general theoretical model and experimental evolution, we tease apart the mechanism that drives their evolution and show that infection risk determines the relative investment in the two arms of defense. ..
  4. Yoganand K, Sivathanu R, Nimkar S, Anand B. Asymmetric positioning of Cas1-2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system. Nucleic Acids Res. 2017;45:367-381 pubmed publisher
    ..The universally conserved Cas1 and Cas2 form an integration complex that is known to mediate the protospacer invasion into the CRISPR array...
  5. Moch C, Fromant M, Blanquet S, Plateau P. DNA binding specificities of Escherichia coli Cas1-Cas2 integrase drive its recruitment at the CRISPR locus. Nucleic Acids Res. 2017;45:2714-2723 pubmed publisher
    ..This integrative mechanism, called adaptation, needs both Cas1 and Cas2 proteins...