Gene Symbol: ygbF
Description: CRISPR adaptation ssRNA endonuclease
Alias: ECK2749, JW5438, cas2
Species: Escherichia coli str. K-12 substr. MG1655
Beloglazova N, Brown G, Zimmerman M, Proudfoot M, Makarova K, Kudritska M, et al
. A novel family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeats. J Biol Chem. 2008;283:20361-71 pubmed publisher
..All CRISPR systems contain proteins of the CAS2 family, suggesting that these uncharacterized proteins play a central role in this process...
Brouns S, Jore M, Lundgren M, Westra E, Slijkhuis R, Snijders A, et al
. Small CRISPR RNAs guide antiviral defense in prokaryotes. Science. 2008;321:960-4 pubmed publisher
..Our results demonstrate that the formation of mature guide RNAs by the CRISPR RNA endonuclease subunit of Cascade is a mechanistic requirement for antiviral defense...
Nakata A, Amemura M, Makino K. Unusual nucleotide arrangement with repeated sequences in the Escherichia coli K-12 chromosome. J Bacteriol. 1989;171:3553-6 pubmed
..Nucleotide sequences hybridizing with the 29-base-pair fragment were also detected in Shigella dysenteriae and Salmonella typhimurium but not in Klebsiella pneumoniae or Pseudomonas aeruginosa. ..
Han K, Park J, Seo H, Ahn K, Lee J. Multiple stressor-induced proteome responses of Escherichia coli BL21(DE3). J Proteome Res. 2008;7:1891-903 pubmed publisher
..The quantitative and systematic proteome analyses that we have performed provide more detailed information on E. coli BL21(DE3), a widely used host strain for recombinant protein overexpression. ..
YOUNG R. Molecular biology. Secret weapon. Science. 2008;321:922-3 pubmed publisher
Nu ez J, Kranzusch P, Noeske J, Wright A, Davies C, Doudna J. Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity. Nat Struct Mol Biol. 2014;21:528-34 pubmed publisher
..The nucleases Cas1 and Cas2 are the only proteins conserved among all CRISPR-Cas systems, yet the molecular functions of these proteins during ..
Wang J, Li J, Zhao H, Sheng G, Wang M, Yin M, et al
. Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems. Cell. 2015;163:840-53 pubmed publisher
..We report on the structures of Cas1-Cas2-dual-forked DNA complexes in an effort toward understanding how the protospacer is sampled prior to insertion into ..
Redding S, Sternberg S, Marshall M, Gibb B, Bhat P, Guegler C, et al
. Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System. Cell. 2015;163:854-65 pubmed publisher
..an alternative pathway that recruits a nuclease-inactive Cas3 through a mechanism that is dependent on the Cas1 and Cas2 proteins...
Wright A, Liu J, Knott G, Doxzen K, Nogales E, Doudna J. Structures of the CRISPR genome integration complex. Science. 2017;357:1113-1118 pubmed publisher
CRISPR-Cas systems depend on the Cas1-Cas2 integrase to capture and integrate short foreign DNA fragments into the CRISPR locus, enabling adaptation to new viruses...