xylE

Summary

Gene Symbol: xylE
Description: D-xylose transporter
Alias: ECK4023, JW3991
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Khankal R, Chin J, Cirino P. Role of xylose transporters in xylitol production from engineered Escherichia coli. J Biotechnol. 2008;134:246-52 pubmed publisher
    ..coli xylose transporters (the d-xylose/proton symporter XylE and the d-xylose ABC transporter XylFGH) and CRP* to xylitol production in the presence of glucose and xylose...
  2. Lam V, Daruwalla K, Henderson P, Jones Mortimer M. Proton-linked D-xylose transport in Escherichia coli. J Bacteriol. 1980;143:396-402 pubmed
    ..Its specificity for xylose as inducer and substrate and the genetic location of a xylose-H+ transport-negative mutation near mtl showed that the xylose-H+ system is distinct from other proton-linked sugar transport systems of E. coli. ..
  3. Sumiya M, Davis E, Packman L, McDonald T, Henderson P. Molecular genetics of a receptor protein for D-xylose, encoded by the gene xylF, in Escherichia coli. Receptors Channels. 1995;3:117-28 pubmed
    Mutants of Escherichia coli K12 have been isolated containing either the 'XylF' or the 'XylE' transport systems for D-xylose...
  4. Quistgaard E, Löw C, Moberg P, Trésaugues L, Nordlund P. Structural basis for substrate transport in the GLUT-homology family of monosaccharide transporters. Nat Struct Mol Biol. 2013;20:766-8 pubmed publisher
    Here we present two structures of the major facilitator (MFS) xylose transporter XylE from Escherichia coli in inward open and partially occluded inward open conformations...
  5. Maiden M, Davis E, Baldwin S, Moore D, Henderson P. Mammalian and bacterial sugar transport proteins are homologous. Nature. 1987;325:641-3 pubmed
    ..There is little apparent homology with the lactose-H+ (LacY) or melibiose-Na+ (MelB) transport proteins of E. coli. ..
  6. Sun L, Zeng X, Yan C, Sun X, Gong X, Rao Y, et al. Crystal structure of a bacterial homologue of glucose transporters GLUT1-4. Nature. 2012;490:361-6 pubmed publisher
    ..Here we report three related crystal structures of XylE, an Escherichia coli homologue of GLUT1-4, in complex with d-xylose, d-glucose and 6-bromo-6-deoxy-D-glucose, at ..
  7. Wambo T, CHEN L, Phelix C, Perry G. Affinity and path of binding xylopyranose unto E. coli xylose permease. Biochem Biophys Res Commun. 2017;494:202-206 pubmed publisher
    ..The E. coli xylose permease (XylE), a homologue of human GLUTs, has been investigated more thoroughly than other major facilitator proteins in the ..
  8. Ke M, Yuan Y, Jiang X, Yan N, Gong H. Molecular determinants for the thermodynamic and functional divergence of uniporter GLUT1 and proton symporter XylE. PLoS Comput Biol. 2017;13:e1005603 pubmed publisher
    GLUT1 facilitates the down-gradient translocation of D-glucose across cell membrane in mammals. XylE, an Escherichia coli homolog of GLUT1, utilizes proton gradient as an energy source to drive uphill D-xylose transport...
  9. Cunningham P, Naftalin R. Reptation-induced coalescence of tunnels and cavities in Escherichia Coli XylE transporter conformers accounts for facilitated diffusion. J Membr Biol. 2014;247:1161-79 pubmed publisher
    Structural changes and xylose docking to eight conformers of Escherichia Coli XylE, a xylose transporter similar to mammalian passive glucose transporters GLUTs, have been examined...

More Information

Publications19

  1. Wisedchaisri G, Park M, Iadanza M, Zheng H, Gonen T. Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE. Nat Commun. 2014;5:4521 pubmed publisher
    ..The proton-coupled sugar transporter XylE is the first member of the MFS that has been structurally characterized in multiple transporting conformations, ..
  2. Jojima T, Omumasaba C, Inui M, Yukawa H. Sugar transporters in efficient utilization of mixed sugar substrates: current knowledge and outlook. Appl Microbiol Biotechnol. 2010;85:471-80 pubmed publisher
    ..Subsequently, we review and discuss recent studies on engineering of sugar transport as a driving force for efficient bioconversion of sugar mixtures derived from lignocellulose. ..
  3. Patrick W, Quandt E, Swartzlander D, Matsumura I. Multicopy suppression underpins metabolic evolvability. Mol Biol Evol. 2007;24:2716-22 pubmed
    ..This genome-wide survey demonstrates that multifunctional genes are common and illustrates the mechanistic diversity by which their products enhance metabolic robustness and evolvability. ..
  4. Saidijam M, Bettaney K, Szakonyi G, Psakis G, Shibayama K, Suzuki S, et al. Active membrane transport and receptor proteins from bacteria. Biochem Soc Trans. 2005;33:867-72 pubmed
  5. Davis E, Jones Mortimer M, Henderson P. Location of a structural gene for xylose-H+ symport at 91 min on the linkage map of Escherichia coli K12. J Biol Chem. 1984;259:1520-5 pubmed
    ..The site of prophage insertion was mapped to 91.4 min on the E. coli genome between pgi and malB. It is proposed that the gene for the xylose-H+ symport system be called xylE.
  6. Francoz E, Dassa E. 3' end of the malEFG operon in E.coli: localization of the transcription termination site. Nucleic Acids Res. 1988;16:4097-109 pubmed
    ..Consequently malG is the last gene of the malEFG operon. orf2 corresponds exactly to the 5' part of the xylE gene reported independently (Davis & Henderson, 1987) as the gene coding for the XylE protein, the xylose-..
  7. Bazzone A, Madej M, Kaback H, Fendler K. pH Regulation of Electrogenic Sugar/H+ Symport in MFS Sugar Permeases. PLoS ONE. 2016;11:e0156392 pubmed publisher
    ..electrophysiological study of the lactose permease (LacY), the fucose permease (FucP) and the xylose permease (XylE), which reveals common mechanistic principles and differences...
  8. Griffith J, Baker M, Rouch D, Page M, Skurray R, Paulsen I, et al. Membrane transport proteins: implications of sequence comparisons. Curr Opin Cell Biol. 1992;4:684-95 pubmed
    ..Their sequence similarities imply a common mechanism of action. This hypothesis provides an objective basis for examining their mechanisms of action and relationships to other transporters. ..
  9. Västermark Ã, Driker A, Li J, Saier M. Conserved movement of TMS11 between occluded conformations of LacY and XylE of the major facilitator superfamily suggests a similar hinge-like mechanism. Proteins. 2015;83:735-45 pubmed publisher
    ..Transmembrane segments (TMSs) 11 in both LacY and XylE of the major facilitator superfamily uniquely contribute the greatest amount of mobile surface area in the outward-..
  10. Davis E, Henderson P. The cloning and DNA sequence of the gene xylE for xylose-proton symport in Escherichia coli K12. J Biol Chem. 1987;262:13928-32 pubmed
    The gene xylE, coding for xylose-proton symport in Escherichia coli, was cloned and its DNA sequence determined. The cloning strategy utilized lambda placMu insertions and exploited the proximity of xylE to malB. A 2...