Gene Symbol: waaY
Description: lipopolysaccharide core biosynthesis protein
Alias: ECK3615, JW3600, rfaY
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Parker C, Kloser A, Schnaitman C, Stein M, Gottesman S, Gibson B. Role of the rfaG and rfaP genes in determining the lipopolysaccharide core structure and cell surface properties of Escherichia coli K-12. J Bacteriol. 1992;174:2525-38 pubmed
    ..A model is presented which relates the deep rough phenotype to the loss of heptose-linked P and PPEA. ..
  2. Klena J, Pradel E, Schnaitman C. Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium. J Bacteriol. 1992;174:4746-52 pubmed
    ..The first two of these, which encode proteins of 27,441 and 32,890 Da, were identified as rfaY and rfaZ by homology of the derived protein sequences of their products to the products of similar genes of ..
  3. Heinrichs D, Yethon J, Whitfield C. Molecular basis for structural diversity in the core regions of the lipopolysaccharides of Escherichia coli and Salmonella enterica. Mol Microbiol. 1998;30:221-32 pubmed
  4. Lee J, Lee K, Yeo W, Park S, Roe J. SoxRS-mediated lipopolysaccharide modification enhances resistance against multiple drugs in Escherichia coli. J Bacteriol. 2009;191:4441-50 pubmed publisher
    ..This induction was dependent on SoxRS, a superoxide and nitric oxide sensing system, through a soxbox in the waaY promoter that binds SoxS...
  5. Schnaitman C, Parker C, Klena J, Pradel E, Pearson N, Sanderson K, et al. Physical maps of the rfa loci of Escherichia coli K-12 and Salmonella typhimurium. J Bacteriol. 1991;173:7410-1 pubmed
  6. Schnaitman C, Klena J. Genetics of lipopolysaccharide biosynthesis in enteric bacteria. Microbiol Rev. 1993;57:655-82 pubmed
  7. Reeves P, Hobbs M, Valvano M, Skurnik M, Whitfield C, Coplin D, et al. Bacterial polysaccharide synthesis and gene nomenclature. Trends Microbiol. 1996;4:495-503 pubmed
    ..We propose a scheme applicable to all species that distinguishes different classes of genes, provides a single name for all genes of a given function and greatly facilitates comparative studies. ..
  8. Yethon J, Heinrichs D, Monteiro M, Perry M, Whitfield C. Involvement of waaY, waaQ, and waaP in the modification of Escherichia coli lipopolysaccharide and their role in the formation of a stable outer membrane. J Biol Chem. 1998;273:26310-6 pubmed
    The waaY, waaQ, and waaP genes are located in the central operon of the waa (formerly rfa) locus on the chromosome of Escherichia coli...
  9. Bociek K, Ferluga S, Mardirossian M, Benincasa M, Tossi A, Gennaro R, et al. Lipopolysaccharide Phosphorylation by the WaaY Kinase Affects the Susceptibility of Escherichia coli to the Human Antimicrobial Peptide LL-37. J Biol Chem. 2015;290:19933-41 pubmed publisher
    ..Among these, inactivation of the waaY gene, encoding a kinase responsible for heptose II phosphorylation in the LPS inner core, leads to a phenotype with ..

More Information


  1. Klena J, Ashford R, Schnaitman C. Role of Escherichia coli K-12 rfa genes and the rfp gene of Shigella dysenteriae 1 in generation of lipopolysaccharide core heterogeneity and attachment of O antigen. J Bacteriol. 1992;174:7297-307 pubmed
    ..All of these studies indicate that the apparent heterogeneity of E. coli K-12 LPS observed on gels is not an artifact but instead a reflection of functional differences among LPS species. ..
  2. Yethon J, Vinogradov E, Perry M, Whitfield C. Mutation of the lipopolysaccharide core glycosyltransferase encoded by waaG destabilizes the outer membrane of Escherichia coli by interfering with core phosphorylation. J Bacteriol. 2000;182:5620-3 pubmed
    ..Structural analyses of waaG mutant lipopolysaccharide showed that the cause for this phenotype was a decrease in core phosphorylation, an unexpected side effect of the waaG mutation. ..