waaB

Summary

Gene Symbol: waaB
Description: lipopolysaccharide 1,6-galactosyltransferase; UDP-D-galactose:(glucosyl)lipopolysaccharide-1,6-D-galactosyltransferase
Alias: ECK3618, JW3603, lps, phx*, rfaB, syn*
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Pradel E, Parker C, Schnaitman C. Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core. J Bacteriol. 1992;174:4736-45 pubmed
    ..It has been designated rfaS. The second gene was identified as rfaB on the basis of its ability to complement a Salmonella typhimurium rfaB mutant and encodes a 42,060-Da protein...
  2. Austin E, Graves J, Hite L, Parker C, Schnaitman C. Genetic analysis of lipopolysaccharide core biosynthesis by Escherichia coli K-12: insertion mutagenesis of the rfa locus. J Bacteriol. 1990;172:5312-25 pubmed
    Tn10 insertions were selected on the basis of resistance to the lipopolysaccharide (LPS)-specific bacteriophage U3...
  3. Schnaitman C, Klena J. Genetics of lipopolysaccharide biosynthesis in enteric bacteria. Microbiol Rev. 1993;57:655-82 pubmed
    From a historical perspective, the study of both the biochemistry and the genetics of lipopolysaccharide (LPS) synthesis began with the enteric bacteria...
  4. Heinrichs D, Yethon J, Whitfield C. Molecular basis for structural diversity in the core regions of the lipopolysaccharides of Escherichia coli and Salmonella enterica. Mol Microbiol. 1998;30:221-32 pubmed
    Bacterial lipopolysaccharides (LPS) are unique and complex glycolipids that provide characteristic components of the outer membranes of Gram-negative bacteria...
  5. Roncero C, Casadaban M. Genetic analysis of the genes involved in synthesis of the lipopolysaccharide core in Escherichia coli K-12: three operons in the rfa locus. J Bacteriol. 1992;174:3250-60 pubmed
    ..encoding the enzymes responsible for the synthesis of responsible for the synthesis of the lipopolysaccharide (LPS) core has been cloned in vivo by using a mini-Mu vector...
  6. Schnaitman C, Parker C, Klena J, Pradel E, Pearson N, Sanderson K, et al. Physical maps of the rfa loci of Escherichia coli K-12 and Salmonella typhimurium. J Bacteriol. 1991;173:7410-1 pubmed
  7. Traurig M, Misra R. Identification of bacteriophage K20 binding regions of OmpF and lipopolysaccharide in Escherichia coli K-12. FEMS Microbiol Lett. 1999;181:101-8 pubmed
    ..Genetic complementation analysis revealed that the rfaB gene, whose product catalyzes the addition of a galactose residue to the first glucose molecule of the LPS core, is ..
  8. Klena J, Ashford R, Schnaitman C. Role of Escherichia coli K-12 rfa genes and the rfp gene of Shigella dysenteriae 1 in generation of lipopolysaccharide core heterogeneity and attachment of O antigen. J Bacteriol. 1992;174:7297-307 pubmed
    ..coli K-12 and Salmonella typhimurium LT2 have been studied to determine their relationship to lipopolysaccharide (LPS) core heterogeneity and their role in the attachment of O antigen to LPS...
  9. Klena J, Pradel E, Schnaitman C. Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium. J Bacteriol. 1992;174:4746-52 pubmed
    ..We speculate that protein RfaL may interact in a strain- or species-specific way with one or more Rfb proteins in the expression of surface O antigen. ..

More Information

Publications15

  1. Walker S, Redman J, Elimelech M. Role of Cell Surface Lipopolysaccharides in Escherichia coli K12 adhesion and transport. Langmuir. 2004;20:7736-46 pubmed
    The influence of bacterial surface lipopolysaccharides (LPS) on cell transport and adhesion has been examined by use of three mutants of Escherichia coli K12 with well-characterized LPS of different lengths and molecular composition...
  2. Qian J, Garrett T, Raetz C. In vitro assembly of the outer core of the lipopolysaccharide from Escherichia coli K-12 and Salmonella typhimurium. Biochemistry. 2014;53:1250-62 pubmed publisher
    ..Six outer core glycosyltransferases, E. coli K-12 WaaG, WaaB, and WaaO and Salmonella typhimurium WaaI, WaaJ, and WaaK, were cloned, overexpressed, and purified...
  3. Pradel E, Schnaitman C. Effect of rfaH (sfrB) and temperature on expression of rfa genes of Escherichia coli K-12. J Bacteriol. 1991;173:6428-31 pubmed
    ..In addition, expression of the lacZ fusion to rfaJ was reduced by growth at 42 degrees C, and this correlated with a temperature-induced change in the electrophoretic profile of the core lipopolysaccharide. ..
  4. Creeger E, Schulte T, Rothfield L. Regulation of membrane glycosyltransferases by the sfrB and rfaH genes of Escherichia coli and Salmonella typhimurium. J Biol Chem. 1984;259:3064-9 pubmed
    ..typhimurium galactosyltransferase. Taken together, the results indicate that the sfrB(rfaH) gene acts as a positive regulatory element in expression of multiple glycosyltransferases in E. coli and S. typhimurium. ..
  5. Parker C, Kloser A, Schnaitman C, Stein M, Gottesman S, Gibson B. Role of the rfaG and rfaP genes in determining the lipopolysaccharide core structure and cell surface properties of Escherichia coli K-12. J Bacteriol. 1992;174:2525-38 pubmed
    Deletions which removed rfa genes involved in lipopolysaccharide (LPS) core synthesis were constructed in vitro and inserted into the chromosome by linear transformation...
  6. Reeves P, Hobbs M, Valvano M, Skurnik M, Whitfield C, Coplin D, et al. Bacterial polysaccharide synthesis and gene nomenclature. Trends Microbiol. 1996;4:495-503 pubmed
    ..We propose a scheme applicable to all species that distinguishes different classes of genes, provides a single name for all genes of a given function and greatly facilitates comparative studies. ..