Gene Symbol: waaA
Description: 3-deoxy-D-manno-octulosonic-acid transferase (KDO transferase)
Alias: ECK3623, JW3608, kdtA
Species: Escherichia coli str. K-12 substr. MG1655
Products:     waaA

Top Publications

  1. Clementz T, Raetz C. A gene coding for 3-deoxy-D-manno-octulosonic-acid transferase in Escherichia coli. Identification, mapping, cloning, and sequencing. J Biol Chem. 1991;266:9687-96 pubmed
    ..The KDO transferase gene, designated kdtA, was subcloned from pLC17-24 into a multicopy vector...
  2. Brabetz W, M ller Loennies S, Brade H. 3-Deoxy-D-manno-oct-2-ulosonic acid (Kdo) transferase (WaaA) and kdo kinase (KdkA) of Haemophilus influenzae are both required to complement a waaA knockout mutation of Escherichia coli. J Biol Chem. 2000;275:34954-62 pubmed publisher
    ..Altmann, K., Lindberg, A. A., Rietschel, E. T., and Zähringer, U. (1988) Eur. J. Biochem. 177, 483-492). The waaA gene encoding the essential LPS-specific Kdo transferase was cloned from this strain, and its nucleotide sequence ..
  3. Raetz C, Reynolds C, Trent M, Bishop R. Lipid A modification systems in gram-negative bacteria. Annu Rev Biochem. 2007;76:295-329 pubmed
    ..Although not required for growth, the modification enzymes modulate virulence of some gram-negative pathogens. Heterologous expression of lipid A modification enzymes may enable the development of new vaccines. ..
  4. Reynolds C, Raetz C. Replacement of lipopolysaccharide with free lipid A molecules in Escherichia coli mutants lacking all core sugars. Biochemistry. 2009;48:9627-40 pubmed publisher
    ..C., et al. (2006) ACS Chem. Biol. 1, 33-42]. Deletion of kdtA, which encodes the Kdo transferase, is possible under these conditions...
  5. Klena J, Ashford R, Schnaitman C. Role of Escherichia coli K-12 rfa genes and the rfp gene of Shigella dysenteriae 1 in generation of lipopolysaccharide core heterogeneity and attachment of O antigen. J Bacteriol. 1992;174:7297-307 pubmed
    ..All of these studies indicate that the apparent heterogeneity of E. coli K-12 LPS observed on gels is not an artifact but instead a reflection of functional differences among LPS species. ..
  6. Clementz T. The gene coding for 3-deoxy-manno-octulosonic acid transferase and the rfaQ gene are transcribed from divergently arranged promoters in Escherichia coli. J Bacteriol. 1992;174:7750-6 pubmed
    The gene kdtA in Escherichia coli codes for 3-deoxy-D-manno-octulosonic acid transferase, the enzyme responsible for attachment of the two 3-deoxy-D-manno-octulosonic acid residues that constitute the link between lipid A and the core ..
  7. Belunis C, Raetz C. Biosynthesis of endotoxins. Purification and catalytic properties of 3-deoxy-D-manno-octulosonic acid transferase from Escherichia coli. J Biol Chem. 1992;267:9988-97 pubmed
    The enzyme 3-deoxy-D-manno-octulosonic acid (Kdo) transferase is encoded by the kdtA gene of Escherichia coli and plays a key role in lipopolysaccharide biosynthesis...
  8. Brozek K, Hosaka K, Robertson A, Raetz C. Biosynthesis of lipopolysaccharide in Escherichia coli. Cytoplasmic enzymes that attach 3-deoxy-D-manno-octulosonic acid to lipid A. J Biol Chem. 1989;264:6956-66 pubmed
    ..The KDO-transferase(s) described in the present study should be very useful for the semisynthetic preparation of complex lipopolysaccharide substructures and analogs. ..
  9. Schnaitman C, Klena J. Genetics of lipopolysaccharide biosynthesis in enteric bacteria. Microbiol Rev. 1993;57:655-82 pubmed

More Information


  1. Belunis C, Clementz T, Carty S, Raetz C. Inhibition of lipopolysaccharide biosynthesis and cell growth following inactivation of the kdtA gene in Escherichia coli. J Biol Chem. 1995;270:27646-52 pubmed
    The enzyme 3-deoxy-D-manno-octulosonic acid (Kdo) transferase is encoded by the kdtA gene in Escherichia coli...
  2. Gronow S, Brabetz W, Brade H. Comparative functional characterization in vitro of heptosyltransferase I (WaaC) and II (WaaF) from Escherichia coli. Eur J Biochem. 2000;267:6602-11 pubmed
    ..transfer of 3-deoxy-Dmanno-oct-2-ulosonic acid (Kdo) and heptose by successive action of Kdo transferase (WaaA), heptosyltransferase I (WaaC) and heptosyltransferase II (WaaF)...
  3. Katz C, Ron E. Dual role of FtsH in regulating lipopolysaccharide biosynthesis in Escherichia coli. J Bacteriol. 2008;190:7117-22 pubmed publisher
    ..Thus, FtsH regulates the concentration of the lipid moiety of LPS (lipid A) as well as the sugar moiety (KDO-based core oligosaccharides), ensuring a balanced synthesis of LPS. ..
  4. Emiola A, Andrews S, Heller C, George J. Crosstalk between the lipopolysaccharide and phospholipid pathways during outer membrane biogenesis in Escherichia coli. Proc Natl Acad Sci U S A. 2016;113:3108-13 pubmed publisher
    ..5.1.108) and WaaA (EC enzymes by the protease FtsH (EC 3.4.24.-)...
  5. Heinrichs D, Yethon J, Whitfield C. Molecular basis for structural diversity in the core regions of the lipopolysaccharides of Escherichia coli and Salmonella enterica. Mol Microbiol. 1998;30:221-32 pubmed