Gene Symbol: uvrD
Description: DNA-dependent ATPase I and helicase II
Alias: ECK3808, JW3786, dar-2, dda, mutU, pdeB, rad, recL, srjC, uvr502, uvrE
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Washburn B, Kushner S. Construction and analysis of deletions in the structural gene (uvrD) for DNA helicase II of Escherichia coli. J Bacteriol. 1991;173:2569-75 pubmed
    DNA helicase II, the product of the uvrD gene, has been implicated in DNA repair, replication, and recombination...
  2. Siegel E. Complementation studies with the repair-deficient uvrD3, uvrE156, and recL152 mutations in Escherichia coli. Mol Gen Genet. 1981;184:526-30 pubmed
    ..For the most part, the different mutations were able to complement each other in respect to traits in which one was deficient and the other had wild type activity. ..
  3. Brosh R, Matson S. Mutations in motif II of Escherichia coli DNA helicase II render the enzyme nonfunctional in both mismatch repair and excision repair with differential effects on the unwinding reaction. J Bacteriol. 1995;177:5612-21 pubmed
    ..The mutant uvrD alleles were also characterized by examining their abilities to complement the mutator and UV light-sensitive ..
  4. Mechanic L, Frankel B, Matson S. Escherichia coli MutL loads DNA helicase II onto DNA. J Biol Chem. 2000;275:38337-46 pubmed
    Previous studies have shown that MutL physically interacts with UvrD (DNA helicase II) (Hall, M. C., Jordan, J. R., and Matson, S. W. (1998) EMBO J...
  5. Matson S. Escherichia coli helicase II (urvD gene product) translocates unidirectionally in a 3' to 5' direction. J Biol Chem. 1986;261:10169-75 pubmed
    Escherichia coli helicase II, product of the uvrD gene, is a single-stranded DNA-dependent nucleoside 5'-triphosphatase with helicase activity. As a DNA-dependent ATPase, helicase II translocates processively along single-stranded DNA (S...
  6. Atkinson J, Guy C, Cadman C, Moolenaar G, Goosen N, McGlynn P. Stimulation of UvrD helicase by UvrAB. J Biol Chem. 2009;284:9612-23 pubmed publisher
    ..b>UvrD is an abundant helicase in Escherichia coli with well characterized functions in mismatch and nucleotide excision ..
  7. Burdett V, Baitinger C, Viswanathan M, Lovett S, Modrich P. In vivo requirement for RecJ, ExoVII, ExoI, and ExoX in methyl-directed mismatch repair. Proc Natl Acad Sci U S A. 2001;98:6765-70 pubmed
    ..associated with 2-aminopurine exposure are effectively suppressed by null mutations in mutH, mutL, mutS, or uvrD/mutU, which encode activities that act upstream of the four exonucleases in the mechanism for the methyl-directed ..
  8. Lestini R, Michel B. UvrD and UvrD252 counteract RecQ, RecJ, and RecFOR in a rep mutant of Escherichia coli. J Bacteriol. 2008;190:5995-6001 pubmed publisher
    Rep and UvrD are two related Escherichia coli helicases, and inactivating both is lethal...
  9. Yamaguchi M, Dao V, Modrich P. MutS and MutL activate DNA helicase II in a mismatch-dependent manner. J Biol Chem. 1998;273:9197-201 pubmed
    ..Although DNA helicase II and Rep helicase share substantial sequence homology and features of mechanism, Rep helicase is inactive in this reaction. ..

More Information


  1. Magner D, Blankschien M, Lee J, Pennington J, Lupski J, Rosenberg S. RecQ promotes toxic recombination in cells lacking recombination intermediate-removal proteins. Mol Cell. 2007;26:273-86 pubmed
    ..coli RecQ can promote the opposite: the net accumulation of BRIs. We report that cells lacking Ruv and UvrD BRI-resolution and -prevention proteins die and display failed chromosome segregation attributable to accumulation ..
  2. SaiSree L, Reddy M, Gowrishankar J. lon incompatibility associated with mutations causing SOS induction: null uvrD alleles induce an SOS response in Escherichia coli. J Bacteriol. 2000;182:3151-7 pubmed
    The uvrD gene in Escherichia coli encodes a 720-amino-acid 3'-5' DNA helicase which, although nonessential for viability, is required for methyl-directed mismatch repair and nucleotide excision repair and furthermore is believed to ..
  3. Ali J, Lohman T. Kinetic measurement of the step size of DNA unwinding by Escherichia coli UvrD helicase. Science. 1997;275:377-80 pubmed
    The kinetic mechanism by which the DNA repair helicase UvrD of Escherichia coli unwinds duplex DNA was examined with the use of a series of oligodeoxynucleotides with duplex regions ranging from 10 to 40 base pairs...
  4. Hall M, Jordan J, Matson S. Evidence for a physical interaction between the Escherichia coli methyl-directed mismatch repair proteins MutL and UvrD. EMBO J. 1998;17:1535-41 pubmed
    b>UvrD (DNA helicase II) is an essential component of two major DNA repair pathways in Escherichia coli: methyl-directed mismatch repair and UvrABC-mediated nucleotide excision repair...
  5. Bidnenko V, Lestini R, Michel B. The Escherichia coli UvrD helicase is essential for Tus removal during recombination-dependent replication restart from Ter sites. Mol Microbiol. 2006;62:382-96 pubmed
    ..In this work, we show that the E. coli helicase II (UvrD) is also essential for the growth of cells where replication forks are arrested at ectopic Ter sites...
  6. Orren D, Sancar A. The (A)BC excinuclease of Escherichia coli has only the UvrB and UvrC subunits in the incision complex. Proc Natl Acad Sci U S A. 1989;86:5237-41 pubmed
    ..Addition of UvrC protein to purified UvrB-DNA complexes results in rapid incision of the DNA, presumably catalyzed by an excision nuclease containing only UvrB and UvrC subunits. ..
  7. Ali J, Maluf N, Lohman T. An oligomeric form of E. coli UvrD is required for optimal helicase activity. J Mol Biol. 1999;293:815-34 pubmed
    Pre-steady-state chemical quenched-flow techniques were used to study DNA unwinding catalyzed by Escherichia coli UvrD helicase (helicase II), a member of the SF1 helicase superfamily...
  8. Flores M, Sanchez N, Michel B. A fork-clearing role for UvrD. Mol Microbiol. 2005;57:1664-75 pubmed
    ..The repair helicase UvrD is essential for replication fork reversal upon inactivation of the polymerase (DnaE) or the beta-clamp (DnaN) ..
  9. Guarné A, Ramon Maiques S, Wolff E, Ghirlando R, Hu X, Miller J, et al. Structure of the MutL C-terminal domain: a model of intact MutL and its roles in mismatch repair. EMBO J. 2004;23:4134-45 pubmed
    ..Each domain of MutL interacts with UvrD helicase and is required for activating the helicase activity...
  10. Ahn B. A physical interaction of UvrD with nucleotide excision repair protein UvrB. Mol Cells. 2000;10:592-7 pubmed
    ..The addition of the UvrD protein to the incised DNA-UvrBC complex results in excision of the incised damaged strand and in the turnover of ..
  11. Heller R, Marians K. Non-replicative helicases at the replication fork. DNA Repair (Amst). 2007;6:945-52 pubmed
    ..Here we examine the DNA-binding specificity of three of the latter group, PriA, Rep, and UvrD. Only PriA and Rep display structure-specific fork binding...
  12. Dao V, Modrich P. Mismatch-, MutS-, MutL-, and helicase II-dependent unwinding from the single-strand break of an incised heteroduplex. J Biol Chem. 1998;273:9202-7 pubmed
    ..MutS and MutL are thus sufficient to coordinate mismatch recognition to the orientation-dependent activation of helicase II unwinding at a single-strand break located a kilobase from the mispair. ..
  13. Lestini R, Michel B. UvrD controls the access of recombination proteins to blocked replication forks. EMBO J. 2007;26:3804-14 pubmed
    ..In Escherichia coli, the UvrD repair helicase was recently shown to act at inactivated replication forks, where it counteracts a deleterious ..
  14. Maluf N, Ali J, Lohman T. Kinetic mechanism for formation of the active, dimeric UvrD helicase-DNA complex. J Biol Chem. 2003;278:31930-40 pubmed
    Escherichia coli UvrD protein is a 3' to 5' SF1 helicase required for DNA repair as well as DNA replication of certain plasmids...
  15. Oh E, Claassen L, Thiagalingam S, Mazur S, Grossman L. ATPase activity of the UvrA and UvrAB protein complexes of the Escherichia coli UvrABC endonuclease. Nucleic Acids Res. 1989;17:4145-59 pubmed
    ..These kinetic changes correlate with changes in footprinting patterns, the stabilization of protein complexes on DNA damage and with the expression of helicase activity. ..
  16. Matson S, Robertson A. The UvrD helicase and its modulation by the mismatch repair protein MutL. Nucleic Acids Res. 2006;34:4089-97 pubmed
    b>UvrD is a superfamily I DNA helicase with well documented roles in excision repair and methyl-directed mismatch repair (MMR) in addition to poorly understood roles in replication and recombination...
  17. Orren D, Selby C, Hearst J, Sancar A. Post-incision steps of nucleotide excision repair in Escherichia coli. Disassembly of the UvrBC-DNA complex by helicase II and DNA polymerase I. J Biol Chem. 1992;267:780-8 pubmed
    ..The discovery of psoralen-UvrB photocross-linking offers the potential of active-site labeling. ..
  18. Veaute X, Delmas S, Selva M, Jeusset J, Le Cam E, Matic I, et al. UvrD helicase, unlike Rep helicase, dismantles RecA nucleoprotein filaments in Escherichia coli. EMBO J. 2005;24:180-9 pubmed
    The roles of UvrD and Rep DNA helicases of Escherichia coli are not yet fully understood. In particular, the reason for rep uvrD double mutant lethality remains obscure...
  19. Fischer C, Maluf N, Lohman T. Mechanism of ATP-dependent translocation of E.coli UvrD monomers along single-stranded DNA. J Mol Biol. 2004;344:1287-309 pubmed
    Escherichia coli UvrD protein is a 3' to 5' SF1 DNA helicase involved in methyl-directed mismatch repair and nucleotide excision repair of DNA...
  20. Lohman T. Escherichia coli DNA helicases: mechanisms of DNA unwinding. Mol Microbiol. 1992;6:5-14 pubmed
    ..coli Rep, RecBCD and phage T7 gene 4 helicases. ..
  21. Oeda K, Horiuchi T, Sekiguchi M. The uvrD gene of E. coli encodes a DNA-dependent ATPase. Nature. 1982;298:98-100 pubmed
  22. Yokota H, Chujo Y, Harada Y. Single-molecule imaging of the oligomer formation of the nonhexameric Escherichia coli UvrD helicase. Biophys J. 2013;104:924-33 pubmed publisher
    ..this study, we addressed this question using direct single-molecule fluorescence visualization of Escherichia coli UvrD, a superfamily I DNA helicase...
  23. Tomko E, Jia H, Park J, Maluf N, Ha T, Lohman T. 5'-Single-stranded/duplex DNA junctions are loading sites for E. coli UvrD translocase. EMBO J. 2010;29:3826-39 pubmed publisher
    Escherichia coli UvrD is a 3'-5' superfamily 1A helicase/translocase involved in a variety of DNA metabolic processes. UvrD can function either as a helicase or only as an single-stranded DNA (ssDNA) translocase...
  24. Maples V, Kushner S. DNA repair in Escherichia coli: identification of the uvrD gene product. Proc Natl Acad Sci U S A. 1982;79:5616-20 pubmed
    ..The low-copy uvrD plasmid (pVMK49) complements a variety of uvrD, uvrE, and recL mutations. In contrast, the same strains carrying the 2...
  25. Brosh R, Matson S. A partially functional DNA helicase II mutant defective in forming stable binary complexes with ATP and DNA. A role for helicase motif III. J Biol Chem. 1996;271:25360-8 pubmed
    ..The DNA-stimulated ATPase activity of UvrDD248N was reduced relative to that of wild-type UvrD with no significant change in the apparent Km for ATP...
  26. Tomko E, Fischer C, Lohman T. Single-stranded DNA translocation of E. coli UvrD monomer is tightly coupled to ATP hydrolysis. J Mol Biol. 2012;418:32-46 pubmed publisher
    Escherichia coli UvrD is an SF1A (superfamily 1 type A) helicase/translocase that functions in several DNA repair pathways...
  27. Lovett S, Sutera V. Suppression of recJ exonuclease mutants of Escherichia coli by alterations in DNA helicases II (uvrD) and IV (helD). Genetics. 1995;140:27-45 pubmed
    ..The uvrD517am mutation does not eliminate uvrD function because it affects UV survival only weakly; moreover, a uvrD insertion mutation could not replace ..
  28. Tomko E, Lohman T. Modulation of Escherichia coli UvrD Single-Stranded DNA Translocation by DNA Base Composition. Biophys J. 2017;113:1405-1415 pubmed publisher
    Escherichia coli UvrD is an SF1A DNA helicase/translocase that functions in chromosomal DNA repair and replication of some plasmids. UvrD can also displace proteins such as RecA from DNA in its capacity as an anti-recombinase...
  29. Washburn B, Kushner S. Characterization of DNA helicase II from a uvrD252 mutant of Escherichia coli. J Bacteriol. 1993;175:341-50 pubmed
    The loss of DNA helicase II (UvrD) in Escherichia coli results in sensitivity to UV light and increased levels of spontaneous mutagenesis...
  30. Tomko E, Fischer C, Niedziela Majka A, Lohman T. A nonuniform stepping mechanism for E. coli UvrD monomer translocation along single-stranded DNA. Mol Cell. 2007;26:335-47 pubmed
    E. coli UvrD is an SF1 helicase involved in several DNA metabolic processes...
  31. Oh E, Grossman L. The effect of Escherichia coli Uvr protein binding on the topology of supercoiled DNA. Nucleic Acids Res. 1986;14:8557-71 pubmed
    ..The extent of this unwinding is of the same order of magnitude as the nucleotide distances of the double incision sites generated by the UvrABC endonucleolytic reaction. ..
  32. Tannenbaum E, Deeds E, Shakhnovich E. Equilibrium distribution of mutators in the single fitness peak model. Phys Rev Lett. 2003;91:138105 pubmed
    ..The repair catastrophe therefore occurs when the repair error probability exceeds the fraction of deleterious mutations. Our model also gives a quantitative estimate for the equilibrium fraction of mutators in Escherichia coli. ..
  33. Wang J, Grossman L. Mutations in the helix-turn-helix motif of the Escherichia coli UvrA protein eliminate its specificity for UV-damaged DNA. J Biol Chem. 1993;268:5323-31 pubmed
    ..Given the highly conserved nature of repair proteins in general, this observation raises the possibility that both procaryotes and eucaryotes might use similar mechanisms to recognize damaged sites in their genomes. ..
  34. Mendonca V, Matson S. Genetic analysis of delta helD and delta uvrD mutations in combination with other genes in the RecF recombination pathway in Escherichia coli: suppression of a ruvB mutation by a uvrD deletion. Genetics. 1995;141:443-52 pubmed
    Helicase II (uvrD gene product) and helicase IV (helD gene product) have been shown previously to be involved in the RecF pathway of recombination...
  35. Mendonca V, Klepin H, Matson S. DNA helicases in recombination and repair: construction of a delta uvrD delta helD delta recQ mutant deficient in recombination and repair. J Bacteriol. 1995;177:1326-35 pubmed
    ..Escherichia coli helicases II (uvrD), IV (helD), and RecQ (recQ) have all been implicated in the presynaptic stage of recombination in the RecF pathway...
  36. Epshtein V, Kamarthapu V, McGary K, Svetlov V, Ueberheide B, Proshkin S, et al. UvrD facilitates DNA repair by pulling RNA polymerase backwards. Nature. 2014;505:372-7 pubmed publisher
    b>UvrD helicase is required for nucleotide excision repair, although its role in this process is not well defined...
  37. An L, Tang W, Ranalli T, Kim H, Wytiaz J, Kong H. Characterization of a thermostable UvrD helicase and its participation in helicase-dependent amplification. J Biol Chem. 2005;280:28952-8 pubmed
    ..Previously, a mesophilic form of HDA (mHDA) utilizing the Escherichia coli UvrD helicase, DNA polymerase I Klenow fragment, two accessory proteins, MutL and single-stranded DNA-binding protein (..
  38. Sukhodolets V. [Formation of the heterozygous tandem duplication in the process of conjugation recombination in Escherichia coli: study of the effect of mutations for the recQ, uvrD, and recJ genes]. Genetika. 1999;35:450-8 pubmed
    ..The effect of mutations for the recQ, uvrD, and recJ genes on the frequency of duplications was studied...
  39. Arthur H, Eastlake P. Transcriptional control of the uvrD gene of Escherichia coli. Gene. 1983;25:309-16 pubmed
    Transcription of the uvrD gene of Escherichia coli was studied using the Mud(Aprlac) gene fusion technique of Casadaban and Cohen [Proc. Natl. Acad. Sci. USA 76 (1979) 4530-4533]...
  40. Finch P, Emmerson P. Nucleotide sequence of the regulatory region of the uvrD gene of Escherichia coli. Gene. 1983;25:317-23 pubmed
    We have sequenced the control region of the Escherichia coli uvrD gene and demonstrated the presence of a nucleotide sequence which is a perfect match for the consensus LexA protein binding site [Little and Mount, Cell 29 (1982) 11-22]...
  41. Maluf N, Fischer C, Lohman T. A Dimer of Escherichia coli UvrD is the active form of the helicase in vitro. J Mol Biol. 2003;325:913-35 pubmed
    The Escherichia coli UvrD protein is a 3' to 5' SF1 DNA helicase involved in methyl-directed mismatch repair and nucleotide excision repair of DNA...
  42. Hwang J, Lee K, Phadtare S, Inouye M. Identification of two DNA helicases UvrD and DinG as suppressors for lethality caused by mutant cspA mRNAs. J Mol Microbiol Biotechnol. 2012;22:135-46 pubmed publisher
    ..We isolated suppressors that contained the genomic fragments encoding uvrD and dinG, respectively, whose gene products are ATP-dependent DNA helicases...
  43. Szwarocka S, Staczek P, Parniewski P. Chromosomal model for analysis of a long CTG/CAG tract stability in wild-type Escherichia coli and its nucleotide excision repair mutants. Can J Microbiol. 2007;53:860-8 pubmed
    ..coli. The absence of the uvrC or uvrD gene products greatly enhances the instability of the TRS in the chromosome, whereas the lack of the functional ..
  44. Wagner K, Moolenaar G, van Noort J, Goosen N. Single-molecule analysis reveals two separate DNA-binding domains in the Escherichia coli UvrA dimer. Nucleic Acids Res. 2009;37:1962-72 pubmed publisher
    ..With ATPgammaS no discrimination between a DNA end and a DNA damage could be observed. We present a model where damage recognition of UvrA depends on the ability of both UvrA monomers to interact with the DNA flanking the lesion. ..
  45. Zhang G, Deng E, Baugh L, Hamilton C, Maples V, Kushner S. Conserved motifs II to VI of DNA helicase II from Escherichia coli are all required for biological activity. J Bacteriol. 1997;179:7544-50 pubmed
    ..Furthermore, a mutation in domain IV (uvrD307, R284A) significantly reduced the viability of some E. coli K-12 strains at 30 degrees C but not at 37 degrees C. The implications of these observations are discussed. ..
  46. Long J, Renzette N, Sandler S. Suppression of constitutive SOS expression by recA4162 (I298V) and recA4164 (L126V) requires UvrD and RecX in Escherichia coli K-12. Mol Microbiol. 2009;73:226-39 pubmed publisher
    ..RecA filament stability is negatively modulated by RecX and UvrD. recA730 (E38K) and recA4142 (F217Y) constitutively express the SOS response...
  47. Boubakri H, de Septenville A, Viguera E, Michel B. The helicases DinG, Rep and UvrD cooperate to promote replication across transcription units in vivo. EMBO J. 2010;29:145-57 pubmed publisher
    ..We report here that in vivo, the DinG, Rep and UvrD helicases are essential for efficient replication across highly transcribed regions...
  48. Majchrzak M, Bowater R, Staczek P, Parniewski P. SOS repair and DNA supercoiling influence the genetic stability of DNA triplet repeats in Escherichia coli. J Mol Biol. 2006;364:612-24 pubmed
    ..These observations suggest a link between the induction of bacterial SOS repair, changes in DNA topology and the mechanisms leading to genetic instability of repetitive DNA sequences. ..
  49. Tham K, Hermans N, Winterwerp H, Cox M, Wyman C, Kanaar R, et al. Mismatch repair inhibits homeologous recombination via coordinated directional unwinding of trapped DNA structures. Mol Cell. 2013;51:326-37 pubmed publisher
    ..Furthermore, we find that the helicase UvrD is recruited to directionally resolve these trapped intermediates toward DNA substrates...
  50. McCool J, Long E, Petrosino J, Sandler H, Rosenberg S, Sandler S. Measurement of SOS expression in individual Escherichia coli K-12 cells using fluorescence microscopy. Mol Microbiol. 2004;53:1343-57 pubmed
    ..The patterns displayed by priA, dnaT, recG, uvrD, dam, ftsK, rnhA, polA and xerC mutants were explained best by the TPM while only lexA (def), lexA3 (ind-) and recA ..
  51. Crowley D, Hanawalt P. The SOS-dependent upregulation of uvrD is not required for efficient nucleotide excision repair of ultraviolet light induced DNA photoproducts in Escherichia coli. Mutat Res. 2001;485:319-29 pubmed
    ..The expression of three NER genes, uvrA, uvrB, and uvrD, is upregulated as part of the SOS response...
  52. Reddy M, Gowrishankar J. Characterization of the uup locus and its role in transposon excisions and tandem repeat deletions in Escherichia coli. J Bacteriol. 2000;182:1978-86 pubmed
    ..into two categories, depending upon whether they did (uup, ssb, polA, and topA) or did not (mutHLS, dam, and uvrD) also increase precise excision frequency of the mini-Tn10 derivatives...
  53. Poteete A. Expansion of a chromosomal repeat in Escherichia coli: roles of replication, repair, and recombination functions. BMC Mol Biol. 2009;10:14 pubmed publisher
    ..Expansion occurred at an increased rate in cells lacking dam, polA, rnhA, or uvrD functions...
  54. Moolenaar G, van Rossum Fikkert S, van Kesteren M, Goosen N. Cho, a second endonuclease involved in Escherichia coli nucleotide excision repair. Proc Natl Acad Sci U S A. 2002;99:1467-72 pubmed
    ..Remaining damages, that for structural reasons obstruct the 3' incision by UvrC, will be repaired by the combined action of Cho (for 3' incision) and UvrC (for 5' incision). ..
  55. Colloms S, Sykora P, Szatmari G, Sherratt D. Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases. J Bacteriol. 1990;172:6973-80 pubmed
    ..The xerC gene has been localized to the 85-min region of the E. coli chromosome, between cya and uvrD. The nucleotide sequences of the xerC gene and flanking regions have been determined...
  56. Feschenko V, Rajman L, Lovett S. Stabilization of perfect and imperfect tandem repeats by single-strand DNA exonucleases. Proc Natl Acad Sci U S A. 2003;100:1134-9 pubmed
    ..we show that the inhibitory effect of ssDNA exonucleases on deletion formation is independent of the MutS and UvrD proteins. Exonuclease degradation of DNA displaced during the deletion process may abort slipped misalignment...
  57. Zou Y, Van Houten B. Strand opening by the UvrA(2)B complex allows dynamic recognition of DNA damage. EMBO J. 1999;18:4889-901 pubmed
    ..These data support the hypothesis that the E.coli and human NER systems recognize and process DNA damage in a highly conserved manner. ..
  58. Flores M, Bidnenko V, Michel B. The DNA repair helicase UvrD is essential for replication fork reversal in replication mutants. EMBO Rep. 2004;5:983-8 pubmed
    ..We report here that the UvrD helicase is essential for RuvABC-dependent chromosome linearization in E...
  59. Selby C, Sancar A. Transcription-repair coupling and mutation frequency decline. J Bacteriol. 1993;175:7509-14 pubmed
  60. Lu A, Clark S, Modrich P. Methyl-directed repair of DNA base-pair mismatches in vitro. Proc Natl Acad Sci U S A. 1983;80:4639-43 pubmed
    ..dependent on ATP, the state of dam methylation of mismatch heteroduplexes, and products of mutH, mutL, mutS, and uvrE loci...
  61. Petranovic M, Zahradka K, Zahradka D, Nagy B, Salaj Smic E, Petranovic D. Genetic evidence that the elevated levels of Escherichia coli helicase II antagonize recombinational DNA repair. Biochimie. 2001;83:1041-7 pubmed
    ..Since helicase II is encoded by the SOS-inducible uvrD gene, SOS-inducing treatments such as irradiating recA(+) or heating recA441 cells were used...
  62. Massoni S, Sandler S. Specificity in suppression of SOS expression by recA4162 and uvrD303. DNA Repair (Amst). 2013;12:1072-80 pubmed publisher
    ..Previous work has shown that one type of recA mutation (e.g., recA4162 (I298V)) and one type of uvrD mutation (e.g...
  63. Yamamoto Y, Ogawa T, Shinagawa H, Nakayama T, Matsuo H, Ogawa H. Determination of the initiation sites of transcription and translation of the uvrD gene of Escherichia coli. J Biochem. 1986;99:1579-90 pubmed
    Prior to the analysis of transcription and translation, the nucleotide sequence of the uvrD gene and its neighboring regions was determined by the method of Maxam and Gilbert (Maxam & Gilbert (1980) Methods Enzymol. 65, 499-560)...
  64. Jia H, Korolev S, Niedziela Majka A, Maluf N, Gauss G, Myong S, et al. Rotations of the 2B sub-domain of E. coli UvrD helicase/translocase coupled to nucleotide and DNA binding. J Mol Biol. 2011;411:633-48 pubmed publisher
    Escherichia coli UvrD is a superfamily 1 DNA helicase and single-stranded DNA (ssDNA) translocase that functions in DNA repair and plasmid replication and as an anti-recombinase by removing RecA protein from ssDNA...
  65. Finch P, Emmerson P. The nucleotide sequence of the uvrD gene of E. coli. Nucleic Acids Res. 1984;12:5789-99 pubmed
    The nucleotide sequence of a cloned section of the E. coli chromosome containing the uvrD gene has been determined...
  66. Selby C, Witkin E, Sancar A. Escherichia coli mfd mutant deficient in "mutation frequency decline" lacks strand-specific repair: in vitro complementation with purified coupling factor. Proc Natl Acad Sci U S A. 1991;88:11574-8 pubmed
    ..We conclude that mfd is, most likely, the gene encoding the transcription-repair coupling factor. ..
  67. Sancar A, Sancar G. DNA repair enzymes. Annu Rev Biochem. 1988;57:29-67 pubmed
  68. Salem A, Nakano T, Takuwa M, Matoba N, Tsuboi T, Terato H, et al. Genetic analysis of repair and damage tolerance mechanisms for DNA-protein cross-links in Escherichia coli. J Bacteriol. 2009;191:5657-68 pubmed publisher
    ..b>UvrD may have an additional role outside NER, since the uvrD mutation conferred a slight azaC sensitivity on cells...
  69. Zhang G, Deng E, Baugh L, Kushner S. Identification and characterization of Escherichia coli DNA helicase II mutants that exhibit increased unwinding efficiency. J Bacteriol. 1998;180:377-87 pubmed
    ..of both ethyl methanesulfonate and site-directed mutagenesis, we have identified a region in DNA helicase II (UvrD) from Escherichia coli that is required for biological function but lies outside of any of the seven conserved ..
  70. Meiners M, Tahmaseb K, Matson S. The UvrD303 hyper-helicase exhibits increased processivity. J Biol Chem. 2014;289:17100-10 pubmed publisher
    ..Escherichia coli helicase II (UvrD) functions in methyl-directed mismatch repair, nucleotide excision repair, and homologous recombination...
  71. Shiraishi K, Imai Y, Yoshizaki S, Tadaki T, Ogata Y, Ikeda H. The role of UvrD in RecET-mediated illegitimate recombination in Escherichia coli. Genes Genet Syst. 2006;81:291-7 pubmed
    ..frequencies of both spontaneous and UV-induced RecET-mediated illegitimate recombination events are reduced by a uvrD mutation...
  72. Ossanna N, Mount D. Mutations in uvrD induce the SOS response in Escherichia coli. J Bacteriol. 1989;171:303-7 pubmed
    We have isolated three new mutations in uvrD that increase expression of the Escherichia coli SOS response in the absence of DNA damage...