Gene Symbol: uvrA
Description: ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC
Alias: ECK4050, JW4019, dar
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Gordienko I, Rupp W. UvrAB activity at a damaged DNA site: is unpaired DNA present?. EMBO J. 1997;16:880-8 pubmed
    To study the activity of the Escherichia coli UvrA and UvrB nucleotide excision repair proteins during the formation of the pre-incision complex at a damaged DNA site, we used substrates with modifications around a single 2-(acetylamino)..
  2. Yang Z, Colis L, Basu A, Zou Y. Recognition and incision of gamma-radiation-induced cross-linked guanine-thymine tandem lesion G[8,5-Me]T by UvrABC nuclease. Chem Res Toxicol. 2005;18:1339-46 pubmed
    ..In Escherichia coli, the excision repair proteins UvrA, UvrB, and UvrC recognize and incise the bulky DNA damages induced by UV light and chemical carcinogens...
  3. Wang H, Lu M, Tang M, Van Houten B, Ross J, Weinfeld M, et al. DNA wrapping is required for DNA damage recognition in the Escherichia coli DNA nucleotide excision repair pathway. Proc Natl Acad Sci U S A. 2009;106:12849-54 pubmed publisher
    ..a mobility shift assay with conformational analysis, we demonstrate that DNA wrapping around UvrB, mediated by UvrA, is an early event in the damage-recognition process during E. coli NER...
  4. Oh E, Claassen L, Thiagalingam S, Mazur S, Grossman L. ATPase activity of the UvrA and UvrAB protein complexes of the Escherichia coli UvrABC endonuclease. Nucleic Acids Res. 1989;17:4145-59 pubmed
    We have analyzed the ATPase activity exhibited by the UvrABC DNA repair complex. The UvrA protein is an ATPase whose lack of DNA dependence may be related to the ATP induced monomer-dimer transitions...
  5. Mellon I. Transcription-coupled repair: a complex affair. Mutat Res. 2005;577:155-61 pubmed
    ..Whether TCR is involved in base excision repair pathways or the repair of common forms of oxidative damage is less clear. This review is focused on the description of possible mechanisms of TCR in E. coli and mammalian cells. ..
  6. Verhoeven E, Wyman C, Moolenaar G, Hoeijmakers J, Goosen N. Architecture of nucleotide excision repair complexes: DNA is wrapped by UvrB before and after damage recognition. EMBO J. 2001;20:601-11 pubmed
    ..In Escherichia coli, damage recognition in NER is accomplished by the UvrA and UvrB proteins...
  7. Verhoeven E, Wyman C, Moolenaar G, Goosen N. The presence of two UvrB subunits in the UvrAB complex ensures damage detection in both DNA strands. EMBO J. 2002;21:4196-205 pubmed
    ..accepted that the damage recognition complex of nucleotide excision repair in Escherichia coli consists of two UvrA and one UvrB molecule, and that in the preincision complex UvrB binds to the damage as a monomer...
  8. Malta E, Moolenaar G, Goosen N. Dynamics of the UvrABC nucleotide excision repair proteins analyzed by fluorescence resonance energy transfer. Biochemistry. 2007;46:9080-8 pubmed
    ..It is the ultimate damage-binding protein that interacts with both UvrA and UvrC. The oligomeric state of UvrB and the UvrAB complex have been subject of debate for a long time...
  9. Orren D, Sancar A. The (A)BC excinuclease of Escherichia coli has only the UvrB and UvrC subunits in the incision complex. Proc Natl Acad Sci U S A. 1989;86:5237-41 pubmed
    The uvrA, uvrB, and uvrC genes control excision repair in Escherichia coli. Cells with mutations in any of these three genes cannot repair DNA by nucleotide excision...

More Information


  1. Gordienko I, Rupp W. The limited strand-separating activity of the UvrAB protein complex and its role in the recognition of DNA damage. EMBO J. 1997;16:889-95 pubmed
  2. Sancar A, Sancar G. DNA repair enzymes. Annu Rev Biochem. 1988;57:29-67 pubmed
  3. Pruteanu M, Baker T. Proteolysis in the SOS response and metal homeostasis in Escherichia coli. Res Microbiol. 2009;160:677-83 pubmed publisher
  4. Grzesiuk E, Gozdek A, Tudek B. Contribution of E. coli AlkA, TagA glycosylases and UvrABC-excinuclease in MMS mutagenesis. Mutat Res. 2001;480-481:77-84 pubmed
    ..Lack of the uvrA gene function did not affect the MMS-induced mutation rate and MFD in AB1157alkA(+)tagA(+)...
  5. Gawel D, Maliszewska Tkaczyk M, Jonczyk P, Schaaper R, Fijalkowska I. Lack of strand bias in UV-induced mutagenesis in Escherichia coli. J Bacteriol. 2002;184:4449-54 pubmed
    ..errors or mutations resulting from the spontaneous SOS mutator effect, measurements of UV-induced mutagenesis in uvrA strains fail to show significant differences between the two target orientations...
  6. Sancar A, Rupp W. A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region. Cell. 1983;33:249-60 pubmed
    The uvrA, uvrB, and uvrC proteins of Escherichia coli were purified from strains that greatly overproduce these proteins. Using the purified proteins, the UVRABC nuclease was reconstituted in vitro...
  7. Orren D, Selby C, Hearst J, Sancar A. Post-incision steps of nucleotide excision repair in Escherichia coli. Disassembly of the UvrBC-DNA complex by helicase II and DNA polymerase I. J Biol Chem. 1992;267:780-8 pubmed
    b>UvrA, UvrB, and UvrC initiate nucleotide excision repair by incising a damaged DNA strand on each side of the damaged nucleotide...
  8. Moolenaar G, van Rossum Fikkert S, van Kesteren M, Goosen N. Cho, a second endonuclease involved in Escherichia coli nucleotide excision repair. Proc Natl Acad Sci U S A. 2002;99:1467-72 pubmed
    ..Remaining damages, that for structural reasons obstruct the 3' incision by UvrC, will be repaired by the combined action of Cho (for 3' incision) and UvrC (for 5' incision). ..
  9. Van Dyk T, DeRose E, Gonye G. LuxArray, a high-density, genomewide transcription analysis of Escherichia coli using bioluminescent reporter strains. J Bacteriol. 2001;183:5496-505 pubmed publisher
    ..As expected, fusions to promoters of LexA-controlled SOS-responsive genes dinG, dinB, uvrA, and ydjM were found to be upregulated in the presence of nalidixic acid...
  10. Rieger R, Zaika E, Xie W, Johnson F, Grollman A, Iden C, et al. Proteomic approach to identification of proteins reactive for abasic sites in DNA. Mol Cell Proteomics. 2006;5:858-67 pubmed
    ..We report identification of seven proteins from Escherichia coli (AroF, DnaK, MutM, PolA, TnaA, TufA, and UvrA) and two proteins from bakers' yeast (ARC1 and Ygl245wp) reactive for AP sites in this system.
  11. Zou Y, Van Houten B. Strand opening by the UvrA(2)B complex allows dynamic recognition of DNA damage. EMBO J. 1999;18:4889-901 pubmed
    ..bases were examined for incision by the Escherichia coli UvrBC endonuclease in the presence or absence of UvrA. UvrBC formed a pre-incision intermediate with a DNA substrate containing a 6-base bubble structure with 2 unpaired ..
  12. Flores M, Bidnenko V, Michel B. The DNA repair helicase UvrD is essential for replication fork reversal in replication mutants. EMBO Rep. 2004;5:983-8 pubmed
    ..coli polymerase III mutants, whereas its partners in DNA repair (UvrA/B and MutL/S) are not. We conclude that UvrD participates in replication fork reversal in E. coli.
  13. Selby C, Witkin E, Sancar A. Escherichia coli mfd mutant deficient in "mutation frequency decline" lacks strand-specific repair: in vitro complementation with purified coupling factor. Proc Natl Acad Sci U S A. 1991;88:11574-8 pubmed
    ..MFD is abolished by mutations in the uvrA, -B, or -C genes, which prevent excision repair, or by a mfd mutation, which reduces the rate of excision but does ..
  14. Aiub C, Mazzei J, Pinto L, Felzenszwalb I. Participation of BER and NER pathways in the repair of DNA lesions induced at low N-nitrosodiethylamine concentrations. Toxicol Lett. 2004;154:133-42 pubmed
    ..5 ng/mL-36.5 microg/mL, through cell survival, in different single and double Escherichia coli DNA repair mutants (uvrA, uvrB, uvrC, fpg, nth, xthA, fpg/nth, uvrA/fpg, fpg/xthA, mutY, and fpg/mutY), using pre-incubation periods of 90 ..
  15. Moolenaar G, Monaco V, van der Marel G, van Boom J, Visse R, Goosen N. The effect of the DNA flanking the lesion on formation of the UvrB-DNA preincision complex. Mechanism for the UvrA-mediated loading of UvrB onto a DNA damaged site. J Biol Chem. 2000;275:8038-43 pubmed
    ..The introduction of a single strand nick at the 5' incision site completely abolished the UvrA-mediated formation of the UvrB-DNA complex...
  16. Sancar A, Sancar G, Rupp W, Little J, Mount D. LexA protein inhibits transcription of the E. coli uvrA gene in vitro. Nature. 1982;298:96-8 pubmed
  17. Sancar A, Wharton R, Seltzer S, Kacinski B, Clarke N, Rupp W. Identification of the uvrA gene product. J Mol Biol. 1981;148:45-62 pubmed
  18. Croteau D, DellaVecchia M, Wang H, Bienstock R, Melton M, Van Houten B. The C-terminal zinc finger of UvrA does not bind DNA directly but regulates damage-specific DNA binding. J Biol Chem. 2006;281:26370-81 pubmed
    In prokaryotic nucleotide excision repair, UvrA recognizes DNA perturbations and recruits UvrB for the recognition and processing steps in the reaction...
  19. Mazon G, Philippin G, Cadet J, Gasparutto D, Fuchs R. The alkyltransferase-like ybaZ gene product enhances nucleotide excision repair of O(6)-alkylguanine adducts in E. coli. DNA Repair (Amst). 2009;8:697-703 pubmed publisher
    ..The YbaZ protein is shown to interact with UvrA. This factor may thus enhance the efficiency of nucleotide excision repair in a way similar to the Transcription-..
  20. Deaconescu A, Sevostyanova A, Artsimovitch I, Grigorieff N. Nucleotide excision repair (NER) machinery recruitment by the transcription-repair coupling factor involves unmasking of a conserved intramolecular interface. Proc Natl Acad Sci U S A. 2012;109:3353-8 pubmed publisher
    ..of DNA damage, removes the enzyme from the DNA, and recruits the Uvr(A)BC nucleotide excision repair machinery via UvrA binding...
  21. Janowska B, Komisarski M, Prorok P, Sokołowska B, Kuśmierek J, Janion C, et al. Nucleotide excision repair and recombination are engaged in repair of trans-4-hydroxy-2-nonenal adducts to DNA bases in Escherichia coli. Int J Biol Sci. 2009;5:611-20 pubmed
    ..coli wild-type or uvrA, recA, and mutL mutants...
  22. Kosa J, Zdraveski Z, Currier S, Marinus M, Essigmann J. RecN and RecG are required for Escherichia coli survival of Bleomycin-induced damage. Mutat Res. 2004;554:149-57 pubmed
    ..Escherichia coli recA cells were far more sensitive than were uvrA, dam-3, and mutM mutY strains, underscoring the importance of RecA to survival...
  23. Majchrzak M, Bowater R, Staczek P, Parniewski P. SOS repair and DNA supercoiling influence the genetic stability of DNA triplet repeats in Escherichia coli. J Mol Biol. 2006;364:612-24 pubmed
    ..These observations suggest a link between the induction of bacterial SOS repair, changes in DNA topology and the mechanisms leading to genetic instability of repetitive DNA sequences. ..
  24. Arakawa H, Tang M. Recognition and incision of Cr(III) ligand-conjugated DNA adducts by the nucleotide excision repair proteins UvrABC: importance of the Cr(III)-purine moiety in the enzymatic reaction. Chem Res Toxicol. 2008;21:1284-9 pubmed publisher
    ..Together, these results suggest that NER proteins from E. coli recognize the purine-Cr(III) adduct but not the Cr(III)-backbone phosphate complex. ..
  25. Courcelle J, Hanawalt P. Participation of recombination proteins in rescue of arrested replication forks in UV-irradiated Escherichia coli need not involve recombination. Proc Natl Acad Sci U S A. 2001;98:8196-202 pubmed
    ..Following UV irradiation, the recovery of DNA replication requires uvrA and uvrC, as well as recA, recF, and recR...
  26. Poole R, Gibson F, Wu G. The cydD gene product, component of a heterodimeric ABC transporter, is required for assembly of periplasmic cytochrome c and of cytochrome bd in Escherichia coli. FEMS Microbiol Lett. 1994;117:217-23 pubmed
    ..A mutant defective in trxB (adjacent to the cydDC operon, and encoding thioredoxin reductase) was unaffected in cytochrome c or bd assembly. ..
  27. Myles G, Sancar A. Isolation and characterization of functional domains of UvrA. Biochemistry. 1991;30:3834-40 pubmed
    The sequence of Escherichia coli UvrA protein suggests that it may fold into two functional domains each possessing DNA binding and ATPase activities...
  28. Ferrezuelo F, Prieto Alamo M, Jurado J, Pueyo C. Role of DNA repair by (A)BC excinuclease and Ogt alkyltransferase in the final distribution of LacI-d mutations induced by N-butyl-N-nitrosourea in Escherichia coli. Mutagenesis. 1998;13:507-14 pubmed
  29. Kenyon C, Walker G. Expression of the E. coli uvrA gene is inducible. Nature. 1981;289:808-10 pubmed
    b>UvrA+-dependent excision repair is one of the most important systems in Escherichia coli for repairing UV-induced pyrimidine dimers and a variety of other forms of DNA damage...
  30. Husain I, Van Houten B, Thomas D, Sancar A. Sequences of Escherichia coli uvrA gene and protein reveal two potential ATP binding sites. J Biol Chem. 1986;261:4895-901 pubmed
    We have determined the nucleotide sequence of the uvrA gene of Escherichia coli. The coding region of the gene is 2820 base pairs which specifies a protein of 940 amino acids and Mr = 103,874...
  31. Hori M, Ishiguro C, Suzuki T, Nakagawa N, Nunoshiba T, Kuramitsu S, et al. UvrA and UvrB enhance mutations induced by oxidized deoxyribonucleotides. DNA Repair (Amst). 2007;6:1786-93 pubmed
    ..8-hydroxy-2'-deoxyguanosine 5'-triphosphate and 2-hydroxy-2'-deoxyadenosine 5'-triphosphate, were introduced into uvrA, uvrB, and uvrC E. coli strains, and mutations in the chromosomal rpoB gene were analyzed...
  32. Szwarocka S, Staczek P, Parniewski P. Chromosomal model for analysis of a long CTG/CAG tract stability in wild-type Escherichia coli and its nucleotide excision repair mutants. Can J Microbiol. 2007;53:860-8 pubmed
    ..coli. The absence of the uvrC or uvrD gene products greatly enhances the instability of the TRS in the chromosome, whereas the lack of the functional UvrA or UvrB proteins causes substantial stabilization of (CTG/CAG) tracts.
  33. Pruteanu M, Baker T. Controlled degradation by ClpXP protease tunes the levels of the excision repair protein UvrA to the extent of DNA damage. Mol Microbiol. 2009;71:912-24 pubmed publisher
    ..levels of DNA-repair proteins during recovery by studying control of the nucleotide excision repair (NER) protein UvrA. We show that UvrA is induced after UV irradiation and reaches maximum levels between approximately 20 and 120 min ..
  34. Ahn B. A physical interaction of UvrD with nucleotide excision repair protein UvrB. Mol Cells. 2000;10:592-7 pubmed
    ..In this communication, it is shown that UvrA and UvrB are precipitated with UvrD in solution but the UvrAB complex is not...
  35. Wagner K, Moolenaar G, van Noort J, Goosen N. Single-molecule analysis reveals two separate DNA-binding domains in the Escherichia coli UvrA dimer. Nucleic Acids Res. 2009;37:1962-72 pubmed publisher
    The UvrA protein is the initial damage-recognizing factor in bacterial nucleotide excision repair. Each monomer of the UvrA dimer contains two ATPase sites...
  36. Kad N, Wang H, Kennedy G, Warshaw D, Van Houten B. Collaborative dynamic DNA scanning by nucleotide excision repair proteins investigated by single- molecule imaging of quantum-dot-labeled proteins. Mol Cell. 2010;37:702-13 pubmed publisher
    ..To address this problem, we uniquely labeled bacterial UvrA and UvrB with differently colored quantum dots and visualized how they interacted with DNA individually or together ..
  37. Pitsikas P, Polosina Y, Cupples C. Interaction between the mismatch repair and nucleotide excision repair pathways in the prevention of 5-azacytidine-induced CG-to-GC mutations in Escherichia coli. DNA Repair (Amst). 2009;8:354-9 pubmed publisher
    ..Strains deficient in mutL, mutS, uvrA, uvrB or uvrC all showed an increase in mutation in response to 5-azacytidine...
  38. Reardon J, Sancar A. Purification and characterization of Escherichia coli and human nucleotide excision repair enzyme systems. Methods Enzymol. 2006;408:189-213 pubmed
    ..This chapter outlines methods for expression and purification of these essential repair factors and provides protocols for performing each of the in vitro repair assays with either the E. coli or the human excision nuclease. ..
  39. Kraithong T, Channgam K, Itsathitphaisarn O, Tiensuwan M, Jeruzalmi D, Pakotiprapha D. Movement of the ?-hairpin in the third zinc-binding module of UvrA is required for DNA damage recognition. DNA Repair (Amst). 2017;51:60-69 pubmed publisher
    ..In bacterial NER, UvrA is the key protein that detects damage and initiates the downstream NER cascade...
  40. Ferguson G, Battista J, Lee A, Booth I. Protection of the DNA during the exposure of Escherichia coli cells to a toxic metabolite: the role of the KefB and KefC potassium channels. Mol Microbiol. 2000;35:113-22 pubmed
    ..coli DNA against methylglyoxal. By the analysis of cells lacking UvrA, we demonstrate that this repair protein is required for the degradation of the DNA upon methylglyoxal exposure...
  41. Fabisiewicz A, Janion C. DNA mutagenesis and repair in UV-irradiated E. coli K-12 under condition of mutation frequency decline. Mutat Res. 1998;402:59-66 pubmed
    ..effect involves preferential repair of pre-mutagenic lesions situated on the transcribed strand, and is mfd-, and uvrA-dependent...
  42. Allan J, Routledge M, Garner R. The Escherichia coli DNA repair protein UvrA can re-associate with the UvrB: aflatoxin B1-DNA complex in vitro. Mutat Res. 1996;362:261-8 pubmed
    The UvrA and UvrB proteins form part of the UvrABc endonuclease, which is responsible for nucleotide excision repair in Escherichia coli...
  43. Lage C, de Padula M, de Alencar T, da Fonseca Gonçalves S, da Silva Vidal L, Cabral Neto J, et al. New insights on how nucleotide excision repair could remove DNA adducts induced by chemotherapeutic agents and psoralens plus UV-A (PUVA) in Escherichia coli cells. Mutat Res. 2003;544:143-57 pubmed
    ..Herein, we bring new insights to the role of Escherichia coli nucleotide excision repair genes uvrA, uvrB and uvrC in the repair of DNA damage induced by some chemotherapeutic agents and psoralen derivatives plus UV-..
  44. Lin J, Sancar A. A new mechanism for repairing oxidative damage to DNA: (A)BC excinuclease removes AP sites and thymine glycols from DNA. Biochemistry. 1989;28:7979-84 pubmed
    ..We conclude that nucleotide excision repair is an important cellular defense mechanism against oxidizing agents. ..
  45. Ivancić Bacće I, Vlasic I, Cogelja Cajo G, Brcic Kostic K, Salaj Smic E. Roles of PriA protein and double-strand DNA break repair functions in UV-induced restriction alleviation in Escherichia coli. Genetics. 2006;174:2137-49 pubmed
    ..Our genetic analysis showed that UV-induced RA is dependent on the excision repair protein UvrA, the RecA-loading activity of the RecBCD enzyme, and the primosome assembly activity of the PriA helicase and is ..
  46. Gu C, Zhang Q, Yang Z, Wang Y, Zou Y, Wang Y. Recognition and incision of oxidative intrastrand cross-link lesions by UvrABC nuclease. Biochemistry. 2006;45:10739-46 pubmed
    ..Here we employed Escherichia coli NER enzymes, i.e., UvrA, UvrB, and UvrC, to examine the incision efficiency of duplex DNA carrying three different oxidative intrastrand ..
  47. Kovalsky O, Lin C, Grossman L. Selection of monoclonal antibodies for probing of functional intermediates in incision of UV-irradiated DNA by Uvr(A)BC endonuclease from Escherichia coli. Biochim Biophys Acta. 1998;1397:91-101 pubmed
    Monoclonal antibodies (mAbs) were generated that recognize UvrA and UvrB proteins. These proteins are components of the Uvr(A)BC endonuclease, which initiates nucleotide excision repair in Escherichia coli...
  48. Backendorf C, Brandsma J, Kartasova T, van de Putte P. In vivo regulation of the uvrA gene: role of the "-10" and "-35" promoter regions. Nucleic Acids Res. 1983;11:5795-810 pubmed
    The effect of increasing deletions in the uvrA promoter region on the transcriptional efficiency was quantitatively analysed by fusion to the galK structural gene...
  49. Kuipers G, Slotman B, Poldervaart H, van Vilsteren I, Reitsma Wijker C, Lafleur M. The role of nucleotide excision repair of Escherichia coli in repair of spontaneous and gamma-radiation-induced DNA damage in the lacZalpha gene. Mutat Res. 2000;460:117-25 pubmed
    ..Subsequently the DNA was transfected into a fpg(-)uvrA(-) Escherichia coli strain (BH420) and the mutational spectra were compared with the spectra of a fpg(-) E...
  50. Kow Y, Wallace S, Van Houten B. UvrABC nuclease complex repairs thymine glycol, an oxidative DNA base damage. Mutat Res. 1990;235:147-56 pubmed
    ..Thus, UvrABC-mediated nucleotide excision repair appears to play a role in the repair of thymine glycol, an oxidative DNA-base lesion that is produced by ionizing radiation or formed during oxidative respiration. ..
  51. Ozgenc A, Szekeres E, Lawrence C. In vivo evidence for a recA-independent recombination process in Escherichia coli that permits completion of replication of DNA containing UV damage in both strands. J Bacteriol. 2005;187:1974-84 pubmed
    ..The nature of the recA-independent recombination mechanism is not known but could perhaps result from a template-strand-switching, or copy choice, process. ..
  52. Hanada K, Iwasaki M, Ihashi S, Ikeda H. UvrA and UvrB suppress illegitimate recombination: synergistic action with RecQ helicase. Proc Natl Acad Sci U S A. 2000;97:5989-94 pubmed
    ..we show that UV-induced illegitimate recombination is enhanced by mutations of nucleotide excision repair genes, uvrA or uvrB, and partially by uvrC mutation, but not by uvrD mutation...
  53. Zou Y, Bassett H, Walker R, Bishop A, Amin S, Geacintov N, et al. Hydrophobic forces dominate the thermodynamic characteristics of UvrA-DNA damage interactions. J Mol Biol. 1998;281:107-19 pubmed
    The Escherichia coli DNA repair proteins UvrA, UvrB and UvrC work together to recognize and incise DNA damage during the process of nucleotide excision repair (NER)...
  54. Backendorf C, Olsthoorn R, van de Putte P. Superhelical stress restrained in plasmid DNA during repair synthesis initiated by the UvrA, B and C proteins in vitro. Nucleic Acids Res. 1989;17:10337-51 pubmed
    Purified UvrA, UvrB, UvrC, UvrD, PolA and Lig proteins from Escherichia coli have been used to assess the effect of nucleotide excision repair on the conformation of native negatively supercoiled plasmid DNA in an in vitro test system...
  55. Nakano T, Morishita S, Terato H, Pack S, Makino K, Ide H. Repair mechanism of DNA-protein cross-link damage in Escherichia coli. Nucleic Acids Symp Ser (Oxf). 2007;:213-4 pubmed
    ..In the present study, we prepared DNA substrates containing defined DPCs and tested them for repair enzymes. We also examined the sensitivity of Escherichia coli to DPC-inducing agents. ..
  56. Ona K, Courcelle C, Courcelle J. Nucleotide excision repair is a predominant mechanism for processing nitrofurazone-induced DNA damage in Escherichia coli. J Bacteriol. 2009;191:4959-65 pubmed publisher
    ..We demonstrate here that these conditions inhibit replication but are insufficient in duration to induce significant levels of DNA damage. ..
  57. Navaratnam S, Myles G, Strange R, Sancar A. Evidence from extended X-ray absorption fine structure and site-specific mutagenesis for zinc fingers in UvrA protein of Escherichia coli. J Biol Chem. 1989;264:16067-71 pubmed
    The UvrA protein is the damage recognition subunit of the Escherichia coli repair enzyme ABC excision nuclease...
  58. DellaVecchia M, Croteau D, Skorvaga M, Dezhurov S, Lavrik O, Van Houten B. Analyzing the handoff of DNA from UvrA to UvrB utilizing DNA-protein photoaffinity labeling. J Biol Chem. 2004;279:45245-56 pubmed
    ..architecture of nucleotide excision repair intermediates it is necessary to identify the specific domains of UvrA, UvrB, and UvrC that are in close proximity to DNA damage during the repair process...
  59. Shah D, Kelly J, Zhang Y, Dande P, Martinez J, Ortiz G, et al. Evidence in Escherichia coli that N3-methyladenine lesions induced by a minor groove binding methyl sulfonate ester can be processed by both base and nucleotide excision repair. Biochemistry. 2001;40:1796-803 pubmed
    ..defective in base excision repair (tag and/or alkA glycosylases or apn endonuclease), nucleotide excision repair (uvrA), and both base and nucleotide excision repair (tag/alkA/uvrA)...
  60. Morganroth P, Hanawalt P. Role of DNA replication and repair in thymineless death in Escherichia coli. J Bacteriol. 2006;188:5286-8 pubmed
    ..Hydroxyurea experiments and thymine starvation of lexA3 and uvrA DNA repair mutants rule out unbalanced growth, the SOS response, and nucleotide excision repair as explanations for ..
  61. Zou Y, Shell S, Utzat C, Luo C, Yang Z, Geacintov N, et al. Effects of DNA adduct structure and sequence context on strand opening of repair intermediates and incision by UvrABC nuclease. Biochemistry. 2003;42:12654-61 pubmed
    ..was affected by the neighboring DNA sequence context, which, in turn, was the result of differential binding of UvrA to the substrates...
  62. Aertsen A, Van Houdt R, Vanoirbeek K, Michiels C. An SOS response induced by high pressure in Escherichia coli. J Bacteriol. 2004;186:6133-41 pubmed
    ..coli, characterized by the RecA and LexA-dependent expression of uvrA, recA, and sulA. Moreover, it was shown that pressure is capable of triggering lambda prophage induction in E...
  63. Goosen N, Moolenaar G. Role of ATP hydrolysis by UvrA and UvrB during nucleotide excision repair. Res Microbiol. 2001;152:401-9 pubmed
    ..Recognition and cleavage of the damaged DNA is a multistep ATP-dependent reaction that requires the UvrA, UvrB and UvrC proteins...
  64. Lohman T. Escherichia coli DNA helicases: mechanisms of DNA unwinding. Mol Microbiol. 1992;6:5-14 pubmed
    ..coli Rep, RecBCD and phage T7 gene 4 helicases. ..
  65. Zou Y, Crowley D, Van Houten B. Involvement of molecular chaperonins in nucleotide excision repair. Dnak leads to increased thermal stability of UvrA, catalytic UvrB loading, enhanced repair, and increased UV resistance. J Biol Chem. 1998;273:12887-92 pubmed
    b>UvrA is one of the key Escherichia coli proteins involved in removing DNA damage during the process of nucleotide excision repair...
  66. Atkinson J, Guy C, Cadman C, Moolenaar G, Goosen N, McGlynn P. Stimulation of UvrD helicase by UvrAB. J Biol Chem. 2009;284:9612-23 pubmed publisher
    ..Here we show that the nucleotide excision repair proteins UvrA and UvrB can together stimulate UvrD-catalyzed unwinding of a range of DNA substrates containing strand ..
  67. Moolenaar G, Moorman C, Goosen N. Role of the Escherichia coli nucleotide excision repair proteins in DNA replication. J Bacteriol. 2000;182:5706-14 pubmed
    ..Here we show that viability is fully dependent on the presence of functional UvrA, UvrB, and UvrD (helicase II) proteins but does not require UvrC...
  68. Oh E, Grossman L. The effect of Escherichia coli Uvr protein binding on the topology of supercoiled DNA. Nucleic Acids Res. 1986;14:8557-71 pubmed
    The effects of the binding of the E. coli UvrA and UvrB proteins on the linking number (delta L) of superhelical DNA has been measured...
  69. Tubbs J, Latypov V, Kanugula S, Butt A, Melikishvili M, Kraehenbuehl R, et al. Flipping of alkylated DNA damage bridges base and nucleotide excision repair. Nature. 2009;459:808-13 pubmed publisher
    ..pombe homologues Rad13 and Swi10 and biochemical interactions with Escherichia coli UvrA and UvrC combined with structural results reveal that ATLs sculpt alkylated DNA to create a genetic and structural ..
  70. Brandsma J, Stoorvogel J, Van Sluis C, van de Putte P. Effect of lexA and ssb genes, present on a uvrA recombinant plasmid, on the UV survival of Escherichia coli K-12. Gene. 1982;18:77-85 pubmed
    The recombinant plasmid pJA01 contains, besides the uvrA gene, the genes lexA, ubiA and ssb. This plasmid does not fully complement a uvrA mutation in a Rec+ background...
  71. Croteau D, DellaVecchia M, Perera L, Van Houten B. Cooperative damage recognition by UvrA and UvrB: identification of UvrA residues that mediate DNA binding. DNA Repair (Amst). 2008;7:392-404 pubmed publisher
    ..In prokaryotes, the proteins UvrA, UvrB and UvrC orchestrate the recognition and excision of aberrant lesions from DNA...
  72. Wang J, Grossman L. Mutations in the helix-turn-helix motif of the Escherichia coli UvrA protein eliminate its specificity for UV-damaged DNA. J Biol Chem. 1993;268:5323-31 pubmed
    The Escherichia coli UvrA protein possesses a stretch of amino acids, 494 to 513, that matches the consensus sequence of the helix-turn-helix motif of many sequence-specific DNA binding proteins...
  73. Czeczot H, Tudek B, Lambert B, Laval J, Boiteux S. Escherichia coli Fpg protein and UvrABC endonuclease repair DNA damage induced by methylene blue plus visible light in vivo and in vitro. J Bacteriol. 1991;173:3419-24 pubmed
    ..survival of pBR322 DNA treated with MB-light was not significantly reduced when transformed into either fpg-1 or uvrA single mutants compared with that in the wild-type strain...
  74. Mekhovich O, Tang M, Romano L. Rate of incision of N-acetyl-2-aminofluorene and N-2-aminofluorene adducts by UvrABC nuclease is adduct- and sequence-specific: comparison of the rates of UvrABC nuclease incision and protein-DNA complex formation. Biochemistry. 1998;37:571-9 pubmed
    ..Most siginificantly, we find that the rate of UvrB and UvrBC-substrate complex formation correlates with the rate of UvrABC nuclease incision. ..